| 3 | Characterization of cancer stem-like cells in a novel STI571-resistant chronic myeloid leukemia cell line显示文摘Objective:To characterize a novel chronic myeloid leukemia(CML)cell line and to further elucidate the mechanisms of resistance to STI571.Methods:A novel K562 cell line(K562/VP16)was achieved after exposure of the K562 cells to VP16.A small subpopulation(K562/VP16 SP)that was capable of excluding Hoechst 33342 in the K562/VP16 cell line was isolated by flow cytometry sorting.The rest of the K562/VP16 cells were classified as non-SP K562/VP16.The mechanisms involved in K562/VP16 SP cells which became resistant to STI571 were studied.Results:The levels of Bcr-Abl and Abl proteins were similar in the K562 cell line and in non-SP K562/VP16 and K562/VP16 SP cells.The multidrug-resistant gene 1(MDR1)expression of the 170 kDa P-glycoprotein(P-gp)was detected in K562/VP16 non-SP and K562/VP16 SP cells but not in K562 cells.The expression levels of P-gp in the two K562/VP16 cell lines were similar.Compared with non-SP K562/ VP16,the K562/VP16 SP cells were more resistant to STI571.This resistance could hardly be reversed by many multidrug resistance inhibitors.In addition,in vivo study showed that the K562/VP16 SP cells induced tumorigenesis in mice,while the K562/VP16 non-SP cells failed to do so.Conclusion:A novel K562 cell line,K562/VP16,was generated.A small side popula- tion K562/VP16 SP cells,had high resistance to STI571 treatment and more tumorigenic than the K562 cells.It may represent the cancer stem cells of the K562/VP16 cell line. | Baijun Fang Yongping Song Yanli Zhang Quande Lin Xudong Wei | 2007 | The Chinese-German Journal of Clinical Oncology2007，6，4： | 0 |
| 4 | Pluripotent stem cells exhibiting similar characteristics can be isolated from human fetal bone marrow, heart, liver, muscle, lung, derma, kidney, and fat显示文摘Previously,we reported that a cell population derived from human fetal bone marrow(BM),termed here Flk1+CD34−postembryonic pluripotent stem cells(PPSCs)that have the characteristics of mesenchymal stem cells(MSCs),could differentiate into ectodermal,endodermal and mesodermal cell types at the single cell level in vitro,and that these cells could also differentiate into the epithelium of liver,lung,gut,as well as the hematopoietic and endothelial lineages after transplantion into irradiated non-obese diabetic/severe combined immunodeficient(NOD/SCID)mice.In this study,we further isolated pluripotent stem cells from human fetal heart,liver,muscle,lung,derma,kidney,and fat and then analyzed the characteristics and function of these stem cells.It was found that the phenotype of the culture-expanded pluripotent stem cells from different fetal tissues was similar to BM-derived Flk1^(+)CD34^(−)PPSCs,i.e.Flk1 and CD44 positive,GlyA,CD34,CD45,class I-HLA and HLA-DR negative.Morphologically,these cells were fibroblast-like and the doubling time was about 30 h.More importantly,culture-expanded pluripotent stem cells from all these fetal tissues were able to differentiate into cells with morphologic and phenotypic characteristics of adipocytes,osteocytes,neurons,glial cells and hepatocytes.These pluripotent stem cells with characteristics similar to fetal BM-derived Flk1^(+)CD34^(−)PPSCs can be selected and cultured from tissues other than the BM.This phenomenon may help explain the“stem cell plasticity”found in multiple human tissues.In addition,as fetal BM-derived Flk1^(+)CD34^(−)PPSCs,these pluripotent stem cells from different fetal tissues had the capacity for self-renewal and multi-lineage differentiation even after being expanded for more than 40 population doublings in vitro.Thus,they may be an ideal source of stem cells for treatment of inherited or degenerative diseases. | FANG Baijun SONG Yongping ZHAO Chunhua SHI Mingxia LIN Quande | 2007 | Frontiers of Medicine2007，1，2： | 0 |
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