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| 1 | Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent显示文摘Objective A highly sensitive and rapid high‐performance liquid chromatography method with pre‐column derivatization with 4‐fluoro‐7‐nitrobenzofurazan was developed for determination of taurine in biological samples,including plasma,brain,and liver.Methods The optimum derivatization reaction temperature was 70℃,and at this temperature the reaction was complete within 3 min.The derivatized taurine was separated using phosphate buffer (0.02 mol/L,pH 6.0):acetonitrile (84:16,v/v) as the mobile phase at a flow rate of 1.0 mL/min,and a column temperature of 25℃.The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.Results The intra‐ and the inter‐day coefficients of variation for the method were 5.3% and 7.7%,respectively.The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.Conclusion This method can be used to determine the taurine contents in plasma,brain,and liver from normal rats and human plasma. | WANG XiFeng CHI DeFeng SU GuanMin LI Lin SHAO LiHua | 2011 | Biomedical and Environmental Sciences2011，24，5： | 13 |
| 2 | Atomic Fe-Zn dual-metal sites for high-efficiency pH-universal oxygen reduction catalysis显示文摘An effective electrocatalyst being highly active in all pH range for oxygen reduction reaction(ORR)is crucial for energy conversion and storage devices.However,most of the high-efficiency ORR catalysis was reported in alkaline conditions.Herein,we demonstrated the preparation of atomically dispersed Fe-Zn pairs anchored on porous N-doped carbon frameworks(Fe-Zn-SA/NC),which works efficiently as ORR catalyst in the whole pH range.It achieves high half-wave potentials of 0.78,0.85 and 0.72 V in 0.1 M HClO4,0.1 M KOH and 0.1 M phosphate buffer saline(PBS)solutions,respectively,as well as respectable stability.The performances are even comparable to Pt/C.Furthermore,when assembled into a Zn-air battery,the high power density of 167.2 mWcm−2 and 120 h durability reveal the feasibility of Fe-Zn-SA/NC in real energy-related devices.Theoretical calculations demonstrate that the superior catalytic activity of Fe-Zn-SA/NC can be contributed to the lower energy barriers of ORR at the Fe-Zn-N6 centers.This work demonstrates the potential of Fe-Zn pairs as alternatives to the Pt catalysts for efficient catalytic ORR and provides new insights of dual-atom catalysts for other energy conversion related catalytic reactions. | Jie Xu Shuhua Lai Defeng Qi Min Hu Xianyun Peng Yifan Liu Wei Liu Guangzhi Hu Heng Xu Fan Li Chao Li Jia He Longchao Zhuo Jiaqiang Sun Yuan Qiu Shusheng Zhang Jun Luo Xijun Liu | 2021 | Nano Research2021，14，5： | 6 |
| 3 | Monitoring of microbial community structure and succession in the biohydrogen production reactor by denaturing gradient gel electrophoresis(DGGE)显示文摘To study the structure of microbial communities in the biological hydrogen produc- tion reactor and determine the ecological function of hydrogen producing bacteria, anaerobic sludge was obtained from the continuous stirred tank reactor (CSTR) in different periods of time, and the diversity and dynamics of microbial communities were investigated by denaturing gra- dient gel electrophoresis (DGGE). The results of DGGE demonstrated that an obvious shift of microbial population happened from the beginning of star-up to the 28th day, and the ethanol type fermentation was established. After 28 days the structure of microbial community became stable, and the climax community was formed. Comparative analysis of 16S rDNA sequences from reamplifying and sequencing the prominent bands indicated that the dominant population belonged to low G+C Gram-positive bacteria (Clostridium sp. and Ethanologenbacterium sp.), β- proteobacteria (Acidovorax sp.), γ-proteobacteria (Kluyvera sp.), Bacteroides (uncultured bacte- rium SJA-168), and Spirochaetes (uncultured eubacterium E1-K13), respectively. The hydrogen production rate increased obviously with the increase of Ethanologenbacterium sp., Clostridium sp. and uncultured Spirochaetes after 21 days, meanwhile the succession of ethanol type fer- mentation was formed. Throughout the succession the microbial diversity increased however it decreased after 21 days. Some types of Clostridium sp. Acidovorax sp., Kluyvera sp., and Bac- teroides were dominant populations during all periods of time. These special populations were essential for the construction of climax community. Hydrogen production efficiency was de- pendent on both hydrogen producing bacteria and other populations. It implied that the co- metabolism of microbial community played a great role of biohydrogen production in the reactors. | XING Defeng REN Nanqi GONG Manli LI Jianzheng LI Qiubo | 2005 | Science China(Life Sciences)2005，48，2： | 5 |
| 4 | A preliminary analysis of the surface chemistry of atmospheric aerosol particles in a typical urban area of Beijing显示文摘Atmospheric aerosol particle samples were collected using an Ambient Eight Stage(Non-Viable) Cascade Impactor Sampler in a typical urban area of Beijing from 27 th Sep.to 5th Oct.,2009.The surface chemistry of these aerosol particles was analyzed using Static Time of Flight-Secondary Ion Mass Spectrometry(Static TOF-SIMS).The factors influencing surface compositions were evaluated in conjunction with the air pollution levels,meteorological factors,and air mass transport for the sampling period.The results show that a variety of organic ion groups and inorganic ions/ion groups were accumulated on the surfaces of aerosol particles in urban areas of Beijing;and hydrophobic organic compounds with short-or middle-chain alkyl as well as hydrophilic secondary inorganic compounds were observed.All these compounds have the potential to affect the atmospheric behavior of urban aerosol particles.PM1.1–2.1and PM3.3–4.7had similar elements on their surfaces,but some molecules and ionic groups demonstrated differences in Time of Flight-Secondary Ion Mass Spectrometry spectra.This suggests that the quantities of elements varied between PM1.1–2.1and PM3.3–4.7.In particular,more intense research efforts into fluoride pollution are required,because the fluorides on aerosol surfaces have the potential to harm human health.The levels of air pollution had the most significant influence on the surface compositions of aerosol particles in our study.Hence,heavier air pollution was associated with more complex surface compositions on aerosol particles.In addition,wind,rainfall,and air masses from the south also greatly influenced the surface compositions of these urban aerosol particles. | Zhengzheng Zhang Hong Li Hongyan Liu Runxiang Ni Jinjuan Li Liqun Deng Defeng Lu Xueli Cheng Pengli Duan Wenjun Li | 2016 | Journal of Environmental Sciences2016，28，9： | 5 |
| 5 | Effect of puerarin on myocardial perfusion and ventricular wall motion in patients with acute coronary syndrome显示文摘Objective To investigate the effects of puerarin (Pur) on myocardial perfusion and ventricular wall motion in patients with acute coronary syndrome (ACS).Methods Thirty-seven patients with ACS were randomly divided into two groups:conventional treatment group (n= 17,11 males,range of age:32-80 years,average age:60.9±4.9 years) and Pur treatment group (n=20,12 males,range of age:40-76 years,average age:62.7±3.5 years).Patients in the conventional treatment group received standard treatment according to the current guidelines,while patients in the Pur treatment group received intravenous administration of Pur (500 mg/day) for 10 days plus conventional treatment.Real-time myocardial contrast echocardiography (RT-MCE) was performed to evaluate the change in myocardial perfusion index (MPI) and ventricular wall motion index (VWMI) at admission and 10 days after treatment.Results At 10 days after treatment,MPI was significantly higher (P<0.01) and VWMI significantly lower (P<0.01) in the Pur group comparing with those in the conventional group.Conclusions Puerarin might improve myocardial microcirculation perfusion and ventricular wall motion in patients with ACS. | Ling Niu Dongye Li Yong Xia Defeng Pan Xiaoping Wang Yan Yan Li Liang Tongda Xu Cardiovascular Institute of Xuzhou Medical College, Department of Cardiology, Affiliated Hospital, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China | 2008 | Journal of Geriatric Cardiology2008，5，3： | 3 |
| 6 | TOF–SIMS surface analysis of chemical components of size–fractioned urban aerosols in a typical heavy air pollution event in Beijing显示文摘Size–fractioned atmospheric aerosol particles were collected during a typical heavy air pollution event in Beijing. The organic and inorganic components on the surfaces of the samples were analyzed using time–of–flight secondary ion mass spectrometry(TOF–SIMS).The variation characteristics of the surface chemical composition and influencing factors were studied, and the possible sources of these chemical compositions were identified through principal component analysis. The results showed that inorganic components such as crustal elements and sulfate, and organic components such as aliphatic hydrocarbons and oxygen–containing organic groups were present. Some surface components, such as polycyclic aromatic hydrocarbons, heavy metals and fluorides may exert adverse effects on human health. The species and relative percentages of the chemical components varied with particle size, diurnal and pollution progress. During a heavy pollution event, the species and relative percentages of secondary components such as oxygen–containing organic groups and sulfurous compounds increased, indicating that particles aged during this event. The surface chemical composition of the aerosol particles was affected mainly by emissions from coal combustion and motor vehicles. In addition, air pollution, meteorological factors, and air mass transport also exerted a significant effect on the surface chemical composition of aerosol particles. | Wenjun Li Hong Li Jinjuan Li Xueli Cheng Zhengzheng Zhang Fahe Chai Hao Zhang Ting Yang Pengli Duan Defeng Lu Yizhen Chen | 2018 | Journal of Environmental Sciences2018，30，7： | 2 |
| 7 | Heterogeneous reaction of NO_2 on the surface of montmorillonite particles显示文摘The studies on heterogeneous reactions over montmorillonite,which is a typical 2:1 layered aluminosilicate,will benefit to the understanding of heterogeneous reactions on clay minerals.Montmorillonite can be classified as sodium montmorillonite or calcium montmorillonite depending on the cation presented between the different layers.Using diffuse reflectance infrared Fourier transform spectroscopy(DRIFTS),the heterogeneous reaction mechanism of NO2 on the surface of montmorillonite was firstly investigated.Results showed that the reaction of NO2 on the surface of sodium and calcium montmorillonite fit a first-order kinetics,and the reaction duration of calcium montmorillonite was longer than that of sodium montmorillonite under the dry condition.For either sodium or calcium montmorillonite,the uptake coefficient decreased as humidity increased. | Zefeng Zhang Jing Shang Tong Zhu Hongjun Li Defeng Zhao Yingju Liu Chunxiang Ye | 2012 | Journal of Environmental Sciences2012，24，10： | 2 |
| 8 | Views of administrators of translation services显示文摘 | Li Defeng Translation curriculum and pedagogy | 2007 | Target2007，19，1： | 1 |
| 9 | 'It's Always More Difficult than You Plan and Imagine':Teachers' Perceived Difficulties in Introducing the Communicative Approach in South Korea显示文摘 | Li Defeng | | 0，，04： | 1 |
| 10 | ' It's always more difficult than you plan and imagine': teacher' s perceived difficulties in introducing the communicative approach in South Korea显示文摘 | Defeng Li | 1998 | TESOL1998，，32： | 1 |
| 11 | Origin, dissemination and entry of the pandemic Zika viruses显示文摘Zika 病毒(ZIKV ) 是打扫了大多数西方的半球的以前忽视,却最新新兴的 zoonotic 病原体。意外注意在新生儿在在 2015 ZIKV 散布到巴西和美国的炸药和它的稀罕关联引起了世界范围的谎言到头小畸型,提起对公共健康的严肃的挑战。我们证明起源和这些流行 ZIKA 的快传播孤立。种系发生的分析表明最新新兴的 ZIKV 孤立的所有属于亚洲美国人的系。ZIKV E 表面蛋白质的结构的分析建议它可能采用类似的入口机制到另外的 Flavi 病毒相似登革热病毒和韦斯特尼罗河病毒的。 | Huimin Zhang Swaminath Srinivas Defeng Li Youjun Feng | 2016 | Science Bulletin2016，61，10： | 1 |
| 12 | Secondary user selection scheme using adaptive genetic algorithms for cooperative spectrum sensing under correlated shadowing 显示文摘 | Ren Defeng Ge Jianhua Li Jing | 2013 | Wireless Personal Communications2013，71，1： | 1 |
| 13 | Ethanoligenens harbinense gennov, spnov, isolated from molasses wastewater显示文摘 | XING Defeng REN Nanqi LI Qiubo | 2006 | Int J Syst Evol Microbiol2006，56，： | 1 |
| 14 | Continuous hydrogen production of auto-aggregative Ethanoligenens harbinense YUAN-3 under non-sterile condition显示文摘 | Defeng Xing Nanqi Ren Aijie Wang Qiubo Li Yujie Feng Fang Ma | 2007 | International Journal of Hydrogen Energy2007，，5： | 1 |
| 15 | Translator training: What translation students have to say显示文摘 | Li Defeng | 2002 | Meta2002，，4： | 1 |
| 16 | Translator training : What translation students have to say 显示文摘 | Li Defeng | 2002 | Meta2002，47，4： | 1 |
| 17 | Translation curriculum and pedagogy: Views of administrators of translation services 显示文摘 | Li Defeng | 2007 | Target2007，19，1： | 1 |
| 18 | New lower bounds on eigenvalue of the Hadamard product of an M-matrix and its inverse显示文摘 | LI Yaotang CHEN Fubin WANG Defeng | 2009 | Linear Algebra Appl2009，430，： | 1 |
| 19 | Translation curriculum and pedagogy:Views of administrators of translation services 显示文摘 | Li Defeng | 2007 | Target2007，19，1： | 1 |
| 20 | Provenance System Analysis of the Nantun Formation of the Huhehu Sag in Hailar Basin显示文摘Huhehu Sag is a new battlefield for the future oil and gas exploration in the Hailar Basin.At present,exploration of this area stays at a low level and the understanding of the provenance system of the Nantun Formation is comparatively weak.So,to carry out the detailed depiction of the provenance system in the study area is significant for the sedimentary system reconstruction,for the prediction of reservoir distribution and favorable areas.Based on comprehensive analysis of paleogeomorphy,light-heavy mineral and seismic reflection features,sandstone thickness and sandstone percentage,this paper analyzes in detail the provenance system of the Nantun Formation in Huhehu Sag from various angles and levels.It shows that provenances of the Huhehu Sag mainly originated from the eastern Xilinbeier Salient and western Bayan Mountain Uplift.The provenances surrounding the lake basin converged to the center of the basin,among which,the steep slope belt and the gentle slope belt are the main provenances,and the southern uplift is a secondary one.The distribution framework of the provenance fundamentally determines the distribution rules of the depositional system and its sand body developing during the Nantun period. | LI Junhui MENG Qi'an LU Shuangfang ZHU Defeng WU Haibo LIU He ZHANG Dazhi | 2012 | Acta Geologica Sinica(English Edition)2012，86，1： | 1 |
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