|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Development and optimization of thermal contrast amplification lateral flow immunoassays for ultrasensitive HIV p24 protein detection显示文摘Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity approaching that of laboratory-based assays.However,the limit of detection(LOD)of lateral flow immunoassays(LFAs),the most prominent POC diagnostic platform,falls short of that of laboratory protein detection methods such as enzyme-linked immunosorbent assay(ELISA).Here,we report the development and optimization of a thermal contrast amplification(TCA)LFA that will allow ultrasensitive detection of 8 pg/ml p24 protein spiked into human serum at POC,approaching the LOD of a laboratory test.To achieve this aim,we pursued several innovations as follows:(a)defining a new quantitative figure of merit for LFA design based on the specific to nonspecific binding ratio(BR);(b)using different sizes and shapes of gold nanoparticles(GNPs)in the systematic optimization of TCA LFA designs;and(c)exploring new laser wavelengths and power regimes for TCA LFA designs.First,we optimized the blocking buffer for the membrane and running buffer by quantitatively measuring the BR using a TCA reader.The TCA reader interprets the thermal signal(i.e.,temperature)of GNPs within the membrane when irradiated by a laser at the plasmon resonance wavelength of the particle.This process results in higher detection and quantitation of GNPs than in traditional visual detection(i.e.,color intensity).Further,we investigated the effect of laser power(30,100,200 mW),GNP size and shape(30 and 100 nm gold spheres,150 nm gold-silica shells),and laser wavelength(532,800 nm).Applying these innovations to a new TCA LFA design,we demonstrated that 100nm spheres with a 100 mW 532 nm laser provided the best performance(i.e.,LOD=8pg/ml).This LOD is significantly better than that of the current colorimetric LFA and is in the range of the laboratory-based p24 ELISA.In summary,this TCA LFA for p24 protein shows promise for detecting acute HIV infection in POC settings. | Li Zhan Timothy Granade Yilin Liu Xierong Wei Ae Youngpairoj Vickie Sullivan Jeff Johnson John Bischof | 2020 | Microsystems & Nanoengineering2020，6，1： | 3 |
| 2 | The use of sex hormones in women with rheumatological diseases显示文摘 | Li RH Gebbie AE Wong RW | | 0，，06： | 2 |
| 3 | DNA and low titer,helper-free,recombinant AAV prime-boost vaccination for cytomegalovirusinduces an immune response to CMV-pp65 and CMV-IEl in trans-genic HLA A*0201 mice显示文摘 | Gallez-Hawkins G Li X1Franck AE | 2004 | Vaccine2004，23，6： | 1 |
| 4 | Elastin point mutations cause an obstructive vascular disease,supravalvular aortic stenosis显示文摘 | Li DY Toland AE Boak BB | | 0，，07： | 1 |
| 5 | Thalamic shape and connectivity abnormalities in children w ith attention-deficit/hyperactivity disorder显示文摘 | Xia S Li X Kimball AE | 2012 | Psychiatry Res2012，204，2： | 1 |
| 6 | Ceramide is involved in triggering of cardiomyocyte apoptosis induced by ischemia and reperfusion显示文摘 | Bielawaska AE Shapiro JP Jing LI | 1997 | Am J Pathol1997，151，6： | 1 |
| 7 | Quantifyingpattern recognition-based myoelectric control ofmultifunctional transcradial prostheses 显示文摘 | Li GL Schultz AE Kuiken TA | 2010 | IEEETransactions on Neural Systems and RehabilitationEngineering: a Publication of the IEEE Engineeringin Medicine and Biology Society2010，18，2： | 1 |
| 8 | Free fatty acids induce hepatocyte mitochondrial dysfunction in a lysesomal-dependeut fashion:A link between steatesis and liver injury显示文摘 | Li Z Melntyre TM Feldstein AE | 2006 | Gastroenterology2006，130，： | 1 |
| 9 | Potential health impact of nanoparticles显示文摘 | Xia T Li N Ae N | 2009 | Annual Review of Public Health2009，30，： | 1 |
| 10 | Normalization of mineral ion homeostasis by dietary means prevents hyperparathy-roidism, rickets, and osteomalacia, but not alopecia in vitamin D receptor-ablated mice显示文摘 | Li YC Amling M Pirro AE | 1998 | Endocrinology1998，139，10： | 1 |
| 11 | GLUT4 heterozygous knockout mice develop muscle insulin resistance and diabetes显示文摘 | Stenbit AE Tsao TS Li J | 1997 | Nature Med1997，3，： | 1 |
| 12 | Targeted ablation of the vita- min D receptor: an animal model of vitamin D-dependent rickets type II with alopecia显示文摘 | Li YC Pirro AE Amling M | 1997 | Proc Natl Acad Sci USA1997，94，8： | 1 |
| 13 | Anti-cancer, anti-diabetic and other pharmacologic and biological activities of pentagalloylglucose显示文摘 | Zhang J Li L Kim SH Hagerman AE Lü J | 2009 | Pharm Res2009，26，9： | 1 |
| 14 | Sclerosing angiomatoid nodular transformation (SANT) of the spleen : addition of a case with focal CD68 staining and distinctive CT features 显示文摘 | Li L Fisher DA Stanek AE | 2005 | Am J Surg Pathol2005，29，6： | 1 |
| 15 | MRSA epidemic linked to a quicklyspreading colonization and virulence determinant 显示文摘 | Li M Du X Villaruz AE | 2012 | Nat Med2012，18，5： | 1 |
| 16 | Dexrazoxane exposure and risk of secondary acute myeloid leukemia in pediatric oncology patients显示文摘 | Seif AE Walker DM Li Y | 2015 | Pediatr Blood Cancer2015，62，4： | 1 |
| 17 | Sclerosing angiomatoid nodular transformation (SANT) of the spleen: addition of a case with focal CD68 staining and distinctive CT features 显示文摘 | Li L Fisher DA Stanek AE | 2005 | Am J Surg Pathol2005，29，6： | 1 |
| 18 | MRSA epidemic linked to a quickly spreading colonization and virulence determinant显示文摘 | Li M Du X Villaruz AE | 2012 | Nature Medicine2012，18，5： | 1 |
| 19 | Management of adult andpaediatric acute lymphoblastic leukaemia in Asia: resource-stratified guidelines from the Asian Oncology Summit 2013显示文摘 | Yeoh AE Tan D Li CK | 2013 | Lancet Oncol2013，14，12： | 1 |
| 20 | AI-2-dependent gene regulation in Staphylococcus epidermidis 显示文摘 | Li M Villaruz AE Vadyvaloo V | 2008 | BMC Microbiol2008，8，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员