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| 1 | Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients显示文摘AIM: To develop a multiplex reverse transcription polymerase chain reaction (RT-PCR) method detecting cir-culating tumor cells in the peripheral blood of colorectal cancer (CRC) patients. METHODS: Peripheral blood samples were collected from 88 CRC patients and 40 healthy individuals from the blood donors' clinic and subsequently analyzed by multiplex RT-RCR for the expression of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) mRNA. The analysis involved determining the detection rates of CEA, CK20 and EGFR transcripts vs disease stage and overall survival. Median follow-up period was 19 mo (range 8-28 mo). RESULTS: Rates of CEA, CK20 and EGFR detection in CRC patients were 95.5%, 78.4% and 19.3%, respectively. CEA transcripts were detected in 3 healthy volunteer samples (7.5%), whereas all control samples were tested negative for CK20 and EGFR transcripts. The increasing number of positive detections for CEA, CK20 and EGFR transcripts in each blood sample was positively correlated with Astler-Coller disease stage (P< 0.001) and preoperative serum levels of CEA (P=0.029) in CRC patients. Data analysis using Kaplan-Meier estimator documented signif icant differences in the overall survival of the different CRC patient groups as formed according to the increasing number of positivity for CEA, CK20 and EGFR transcripts. CONCLUSION: These data suggest that multiplex RTPCR assay can provide useful information concerning disease stage and overall survival of CRC patients. | Aikaterini Tsouma Chrysanthi Aggeli Panagiotis Lembessis George N Zografos Dimitris P Korkolis Dimitrios Pectasides Maria Skondra Nikolaos Pissimissis Anastasia Tzonou Michael Koutsilieris | 2010 | World Journal of Gastroenterology2010，16，47： | 19 |
| 2 | Detection of circulating tumor cells in bladder cancer patients显示文摘 | NEZOS A PISSIMISIS N LEMBESSIS P | 2009 | Cancer Treat Rev2009，35，27： | 1 |
| 3 | Increased insulin-like growth factor 1 activity can rescue KLE endometrial-like cells from apoptosis显示文摘 | Koutsilieris M Mastrogamvrakis G Lembessis P | 2001 | Mol Med2001，7，1： | 1 |
| 4 | Regulation of the mGluR5, EAAT1 and GS expression by glucocorticoids in MG-63 osteoblast-like osteosarcoma cells 显示文摘 | Kalariti N Lembessis P Papageorgiou E | 2007 | J Musculoskelet Neuronal Interact2007，7，2： | 1 |
| 5 | Detection of circulat- ing tumor cells in bladder cancer patients 显示文摘 | Nezos A Pissimisis N Lembessis P | 2009 | Cancer Treat Rev2009，35，： | 1 |
| 6 | The kisspeptin(Ki SS-1)/GPR54 system in cancer biology显示文摘 | Makri A Pissimissis N Lembessis P | 2008 | Cancer Treat Rev2008，34，8： | 1 |
| 7 | Rosiglitazone attenuates insulin-like growth factor 1 receptor survival signaling in PC-3 cells 显示文摘 | Papageorgiou E Pitulis N Manoussakis M Lembessis P Koutsilieris M | 2008 | Mol Med2008，14，78： | 1 |
| 8 | Detection of circulating tumor cells in prostate cancer patients : methodological pitfalls and clinical relevance显示文摘 | Panteleakou Z Lembessis P Sourla A | 2009 | Mol Med2009，15，34： | 1 |
| 9 | The kisspeptin (KiSS-1)/ GPR54 system in cancer biology显示文摘 | Makri A Pissimissis N Lembessis P | 2008 | Cancer Treat Rev2008，34，8： | 1 |
| 10 | The kisspeptin (KISS- 1)/GPR54 system in cancer biology 显示文摘 | Makri A Pissimissis N Lembessis P | 2008 | Cancer Treat Rev2008，34，8： | 1 |
| 11 | Conversion of nested reverse-transcriptase polymerase chain reaction from positive to negative status at peripheral blood during androgen ablation therapy is associated with long progression-free survival in stage D2 prostate cancer patients 显示文摘 | Sourla A Lembessis P Mitsiades C Dimopoulos T Skouteris M Metsinis M Ntounis A Ioannidis A Katsoulis A Kyragiannis V Lambou T Tsintavis A Koutsilieris M | 2001 | Anticancer Res2001，21，5： | 1 |
| 12 | The kisspeptin (KISS- 1) /GPR54 system in cancer biology显示文摘 | Makri A Pissimissis N Lembessis P | 2008 | Cancer Treat Rev2008，34，8： | 1 |
| 13 | Regulation of the mGluR5, EAAT1 and GS expression by glucocorticoids in MG-63 osteoblast-like osteosarooma cells 显示文摘 | Kalariti N Lembessis P Papageorgiou E | 2007 | J Musculoskelet Neuronal Interact2007，7，2： | 1 |
| 14 | Skeletal Growth Factor Involvement in the Regulation of Fracture Healing Process显示文摘 | GS Themistocleous SE Kontou P Lembessis | 2003 | In vivo2003，17，： | 1 |
| 15 | Increased insulin-like growth factor 1 activity can rescue KLE endometrial-like cells from apoptosis显示文摘 | Koutsilieris M Mastrogamvrakis G Lembessis P | 2001 | Mol Med2001，7，1： | 1 |
| 16 | Detection of circulating tumor cells in bladder cancer patients 显示文摘 | NEZOS A PISSIMISIS N LEMBESSIS P | 2009 | Cancer Treat Rev2009，35，3： | 1 |
| 17 | Molecular staging by RT-PCR analysis for PSA and PSMA in peripheral blood and bone marrow samples is an independent predictor of time to biochemical failure following radical prostatectomy for clinically localized prostate cancer显示文摘 | Mitsiades CS Lembessis P Sourla A | 2004 | Clin Exp Metastasis2004，21，6： | 1 |
| 18 | Increased insulin-like growth factor 1 activity can rescue KLE endometrial-like ceils from apoptosls显示文摘 | Koutsilieris M Mastrogamvrakis G Lembessis P | 2001 | Mol Meal2001，7，1： | 1 |
| 19 | Detection of circulatingtumor cells in prostate cancer patients: methodological pitfalls and clinical relevance 显示文摘 | Panteleakou Z Lembessis P Sourla A | 2009 | Mol Med2009，15，34： | 1 |
| 20 | Molecular staging by RT-PCR analysis for PSA and PSMA in peripheral blood and bone marrow samples is an independent predictor of time to biochemical failure following radical prostatectomy for clinically localized prostate caneer显示文摘 | Mitsiades CS Lembessis P Sourla A | 2004 | Clin Exp Metastasis2004，21，6： | 1 |
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