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| 1 | Keratinocyte 生长 factor-2 和自体同源的浆液加强处于在老鼠的角膜损坏的间充质的干细胞的再生效果显示文摘AIM:To investigate the healing process after severe corneal epithelial damage in rats treated with mesenchymal stem cells(MSCs)cultured with or without keratinocyte growth factor(KGF-2)and autologous serum(AS)on amniotic membrane(AM).Many patients are blind and devastated by severe ocular surface diseases due to limbal stem cell deficiency.Bone marrow-derived MSCs are potential sources for cellbased tissue engineering to repair or replace the corneal tissue,having the potential to differentiate to epithelial cells.METHODS:The study included 5 groups each including 10 female'Sprague Dawley'rats in addition to20 male rats used as bone marrow donors.Group I rats received AM+MSCs,Group II rats AM+MSCs cultured with KGF-2,Group III rats AM+MSCs cultured with KGF-2+AS,Group IV rats only AM and Group V rats,none.AS was derived from blood drawn from male rats and bone marrow was obtained from the femur and tibia bones of the same animals.Therapeutic effect was evaluated with clinical,histopathological and immunohistochemical assessment.MSC engraftment was demonstrated via detection of donor genotype(Y+)in the recipient tissue(X)with polymerase chain reaction.RESULTS:Corneal healing was significantly better in Groups I-III rats treated with MSC transplantation compared to Group IV and Group V rats with supportive treatment only.The best results were obtained in Group III rats with 90%transparency,70%lack of neovascularization,and 100%epithelium damage limited to less than 1/4 of cornea.CONCLUSION:We suggest that culture of MSCs with KGF-2 and AS on AM is effective in corneal repair in case of irreversible damage to limbal stem cells. | Ferda Alpaslan Pinarli Gülsen Okten Umit Beden Tunc Fisgin Mehmet Kefeli Nurten Kara Feride Duru Leman Tomak | 2014 | International Journal of Ophthalmology(English edition)2014，7，2： | 4 |
| 2 | Mahogenic amylase has a non- typical impact on the molecular and rheological properties of starch 显示文摘 | Leman P Goesaert H Vandeputte G E | 2005 | Carbohydrate Polymers2005，62，3： | 1 |
| 3 | Toward Communicating Expressiveness and Affect in Multimodal Interactive Systems for Performing Arts and Cultural Applications显示文摘 | A Camurri G De Poli M Leman | 2005 | IEEE Multimedia2005，12，1： | 1 |
| 4 | Anti-oxidized low-density lipoprotein antibodies in patients with coronary heart disease and normal healthy volunteers显示文摘 | Virella G Virella I Leman RB | 1993 | Int J Clin Lab Res1993，23，2： | 1 |
| 5 | Telesurgical laparo- scopic cholecystectomy 显示文摘 | HIMPENS J LEMAN G CADIERE G B | 1988 | Surg Endose1988，12，8： | 1 |
| 6 | Induction, recovery, and safcfy characteristics of scvoflurane in children underoing ambulatory surgery 显示文摘 | LEMAN J DAVIS P J WELBRON L G | 1996 | Anesthesiology1996，84，： | 1 |
| 7 | Toward communicating expressiveness and affect in multimodal interactive systems for performing arts and cultural applications显示文摘 | Camurri A Poli G D Leman M | 2005 | IEEE Multimedi- a2005，12，1： | 1 |
| 8 | Mutations in a CCHC zinc-binding motif of the reovirus sigma 3 protein decrease its intracellular stability显示文摘 | MABROUK T LEMAN G | 1994 | J Virol1994，68，8： | 1 |
| 9 | Telesurgical laparoscopic cholecystectomy 显示文摘 | HIMPENS J LEMAN G CARDIERE GB | 1998 | Surg Endosc1998，12，8： | 1 |
| 10 | Anti-oxidized low- density lipoprotein antibodies in patients with coronary heart disease and normal healthy volunteers 显示文摘 | Virella G Virella I Leman RB | 1993 | Int J Clin Lab Res1993，23，2： | 1 |
| 11 | Telesurgical laparoseopie choleeystectomy显示文摘 | Himpens J Leman G Cadiere GB | 1998 | Surg Endosc1998，12，： | 1 |
| 12 | Telesurgical laparoscopic eholecystectomy显示文摘 | Himpens J Leman G Cadiere GB | 1998 | Surg Endosc1998，12，： | 1 |
| 13 | Endothelin in human congestive heart failure显示文摘 | WEI G M LEMAN A RODEHEFFER R J | 1994 | Girculation1994，89，4： | 1 |
| 14 | EPCA-2: a highly specific serum marker for prostate cancer显示文摘 | Leman E S Cannon G W TrockB J | 2007 | Urology2007，169，4： | 1 |
| 15 | Telesurgical laparoscopic cholecystectomy 显示文摘 | Himpens J Leman G Cadiere GB | 1998 | Surg Endosc1998，12，8： | 1 |
| 16 | Applica tion of in vitro anthemintic sensitivity assays to canine parasi tology: Detecting resistance to pyrantel in Ancylostoma cani hum 显示文摘 | KOPP S R C()LEMAN G T MCCARTHY J S et a l | 2008 | Vet Parasitol2008，152，34： | 1 |
| 17 | Anti - oxidized low density lipoprotein antibodies in patients with coronary heart disease and normal healthy volunteers显示文摘 | Virella G Virella I Leman RB | 1993 | Int J Clin Lab Res1993，23，： | 1 |
| 18 | EPCA 2: a highly specific serum marker for prostate cancer显示文摘 | Leman E S Cannon G W Trock B J | 2007 | Urology2007，69，： | 1 |
| 19 | Anti-oxidized low- densityllpoprotein antibodies in patients with coronary heart diease andnormal healthy volunteers显示文摘 | VireHa G Virdla I Leman RB | 1993 | Int J Clin Lab Res1993，23，2： | 1 |
| 20 | The use of a colon cancer associated nuclear antigen CCSA-2 for the blood based detection of colon cancer显示文摘 | Walgenbach-Brunagel G Burger B Leman ES | 2008 | J Cell Biochem2008，104，1： | 1 |
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