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| 1 | Ethyl acetate fraction in ethanol extract from root of 'Dai-Bai-Jie' (Marsdenia tenacissima):anti-tumor activity in A549cancer cells显示文摘OBJECTIVE: To evaluate the anti-tumor activity of ethyl acetate fraction(EFA), extracted with ethanol from the root of 'Dai-Bai-Jie' in A549 cancer cells and its underlying mechanism.METHODS: 'Dai-Bai-Jie' was extracted with 95%ethanol-aqueous(DBJ-1), 50% ethanol-aqueous(DBJ-2), and water(DBJ-3) by reflux method. 95%ethanol-aqueous extract was separated byethyl acetate(EFA) and n-butyl alcohol(DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related pro-teins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in vivo.RESULTS: DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC5025 and 3.5 μg/mL, respectively. EFA can exhibit the proliferation,arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.CONCLUSION: EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53,Caspase-3, and Caspase-8 proteins. | Li Xiaohua Li Haitao Jin Lingyu Chen Leixi Niu Yingfeng Guan Yanhong Ma Xiaojun Zhang Lixia | 2018 | Journal of Traditional Chinese Medicine2018，38，5： | 1 |
| 2 | Sequencingandanalysisofthecompletegenomeofranagryliovirus(rgv)显示文摘 | LEIXY OUT ZHURL etal | 2012 | ArchVirol2012，157，8： | 1 |
| 3 | 基于生物复合材料的双曲面分段式壳体展馆显示文摘生物复合材料由于成本低、可再生和对环境友好的特性,在建筑中获得了新颖又广泛的应用。通过一对一的双曲面、参数化设计形成的分段式壳体,来展示生物材料在承重结构中的应用。这种结构由轻质的单向弯曲木和生物复合材料组成,其中,木质纤维基核心由长木纤维以单板形式加固。进一步探讨了高3.6m,面积55m^2的展馆的建造技术以及生物复合材料应用的可能性。 | 汉娜·达希 简·彼得斯 皮奥特·巴辛斯基 米夏拉·梅 ZHANG Zhiqing(Translator) QIAN Leixi(Proofreader) | 2019 | 风景园林2019，26，8： | 0 |
| 4 | Local low-dose X-ray radiation promotes homing of mesenchymal stem cells to the injured mouse spinal cord显示文摘BACKGROUND: Bone marrow-derived mesenchymal stem cells (BMSCs) are a potentially useful source for cell replacement therapy following spinal cord injury.However,the homing characteristics of BMSCs in vivo remain unclear.Low-dose radiation has been shown to promote homing of BMSCs to exposed sites.OBJECTIVE: To investigate the effects of low-dose local radiation to non-injured areas on the ability of human BMSCs to home to the injured mouse spinal cord,as well as recovery of spinal cord injury.DESIGN,TIME AND SETTING: A randomized,controlled,animal experiment was performed at the Central Laboratory,Second Affiliated Hospital of Soochow University between October 2007 and October 2008.MATERIALS: BMSCs were isolated from four adult,human donors.METHODS: Fifty adult,female,Balb/c mice were subjected to adjusted weight-drop impact resulting in complete paraplegia.Three days later,mice were randomly assigned to a radiation + transplantation group (n = 23) and a transplantation group (n = 20).In total,2 × 106 carboxyfluorescein diacetate succinimidyl ester-labeled BMSCs were injected into each mouse via the caudal vein.Mice in the radiation + transplantation group received 2.5 Gy local X-ray irradiation 2 hours before BMSCs injection.MAIN OUTCOME MEASURES: The homing of BMSCs to injured cord and irradiated skin after transplantation was observed by fluorescence microscope;the structure recovery of injured cord was assessed by magnetic resonance imaging.RESULTS: Compared with the transplantation group,at 24 hours after transplantation,the number of BMSCs was significantly increased in the injured area and the exposed site (P < 0.05),and inflammation and edema were significantly alleviated in the injured cord in the radiation + transplantation group.CONCLUSION: Local low-dose radiation has the potential to promote homing of BMSCs and recovery of spinal cord injury,although the radiated region was not injured area. | Panjun Wang Xiaohui Zhang Leixi Xue Yu Sun Songguang Ju Liesong Chen Jiasheng Hu Hong Zhang Xueguang Zhang Jinxiang Fu | 2010 | Neural Regeneration Research2010，5，5： | 0 |
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