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| 1 | 利用铅同位素比值判断丹参不同产地来源显示文摘建立了用微柱流动注射与电感耦合等离子体质谱联用的铅同位素比值测量方法。利用铅与 5 磺基 8 羟基喹啉的螯合反应在线分离测定丹参样品中的铅同位素比值 ,并利用铅同位素比值的分布进行中药丹参产地来源的研究。实验结果表明 ,相同地区来源的丹参样品其铅同位素比值分布相对集中 ,而不同地区来源的丹参 ,铅的同位素比值则有明显的区别。因此 。 | 黄志勇 杨妙峰 庄峙厦 王小如 Frank S C Lee | 2003 | 分析化学2003，31，9： | 30 |
| 2 | 加速溶剂萃取-高效液相色谱-电喷雾飞行时间质谱联用分析莲子心中生物碱显示文摘建立了加速溶剂萃取-高效液相色谱-电喷雾飞行时间质谱(ASE-HPLC-DAD-ESI-TOF/MS)联用技术分析莲子心中生物碱类化合物的方法。采用ASE法对药材进行提取,萃取温度为100℃,压力为9646kPa,时间为10min。采用HPLC-DAD-ESI-TOF/MS联用技术对其中的化学成分进行了分离鉴定,HPLC采用AgilentEclipseXDB-C18色谱柱;流动相为0.1%三乙胺溶液,用氨水调pH=8.0(A),色谱纯乙腈(B),梯度洗脱;进样量为20μL,流速:1.0mL/min;检测波长:282nm。ESI-TOF/MS采用正离子电离模式,毛细管电压:4500V;喷雾气压:310.05kPa;干燥气(N2)流速:10.0L/min;干燥气温度:350℃,破碎电压:150V。鉴定了其中的6种生物碱分别为:前荷叶碱、莲心季铵碱、莲心碱、异莲心碱、荷叶碱和甲基莲心碱。 | 李文龙 陈军辉 殷月芬 王小如 Frank S C Lee | 2008 | 分析化学2008，36，1： | 24 |
| 3 | Ten-year survival of hepatocellular carcinoma patients undergoing radiofrequency ablation as a first-line treatment显示文摘AIM: To investigate the long-term survival and prognostic factors in hepatocellular carcinoma(HCC) patients undergoing radiofrequency ablation(RFA) as a first-line treatment.METHODS: From 2000 to 2013, 316 consecutivepatients with 404 HCC(1.0-5.0 cm; mean: 3.2 ± 1.1 cm) underwent ultrasonography-guided percutaneous RFA as a first-line treatment. There were 250 males and 66 females with an average age of 60.1 ± 10.8 years(24-87 years). Patients were followed for 1 year to > 10 years after RFA(234, 181, 136, and 71 for 3, 5, 7, and 10 years, respectively). Overall local response rates and long-term survival rates were assessed. Survival results were generated using Kaplan-Meier estimates, and multivariate analysis was performed using the Cox regression model. RESULTS: In total, 548 RFA sessions were performed and major complications occurred in 10 sessions(1.8%). Local tumor progression and/or new tumor development were observed in 43.3%(132/305) of the patients during the follow-up period. Overall 5-and 10-year survival rates were 49.7% and 28.4%, respectively. Based on multivariate analysis, three factors were identified as independent prognostic factors for overall survival: Child-Pugh classification(HR = 4.054, P < 0.001), portal vein hypertension(HR = 2.743, P = 0.002), and tumor number(HR = 2.693, P = 0.003). The local progression-free 5- and 10-year survival rates were 42.7% and 19.5%. In addition to the Child-Pugh classification and the number of tumors, the number of RFA sessions(HR = 1.550, P = 0.002) was associated with local progression-free survival.CONCLUSION: RFA can achieve acceptable outcomes for HCC patients as a first-line treatment, especially for patients with Child-Pugh class A, patients with a single tumor and patients without portal vein hypertension. | Wei Yang Kun Yan S Nahum Goldberg Muneeb Ahmed Jung-Chieh Lee Wei Wu Zhong-Yi Zhang Song Wang Min-Hua Chen | 2016 | World Journal of Gastroenterology2016，22，10： | 20 |
| 4 | 气相色谱法在中草药及其制剂中痕量有机氯类农药残留分析上的应用进展显示文摘综述了中草药及其制剂中有机氯类农药残留的气相色谱分析技术,包括样品前处理方法,气相色谱分离分析及确证鉴定技术,对近几年来该领域研究进展进行了总结和讨论。 | 徐晓琴 胡广林 王小如 LEE F S C | 2004 | 分析测试学报2004，23，4： | 15 |
| 5 | Management of distal humeral coronal shear fractures显示文摘Coronal shear fractures of the distal humerus are rare,complex fractures that can be technically challenging to manage. They usually result from a low-energy fall and direct compression of the distal humerus by the radial head in a hyper-extended or semi-flexed elbow or from spontaneous reduction of a posterolateral subluxation or dislocation. Due to the small number of soft tissue attachments at this site, almost all of these fractures are displaced. The incidence of distal humeral coronal shear fractures is higher among women because of the higher rate of osteoporosis in women and the difference in carrying angle between men and women. Distal humeral coronal shear fractures may occur in isolation, may be part of a complex elbow injury, or may be associated with injuries proximal or distal to the elbow. An associated lateral collateral ligament injury is seen in up to 40% and an associated radial head fracture is seen in up to 30% of these fractures. Given the complex nature of distal humeral coronal shear fractures, there is preference for operative management. Operative fixation leads to stable anatomic reduction, restores articular congruity, and allows initiation of early range-of-motion movements in the majority of cases. Several surgical exposure and fixation techniques are available to reconstruct the articular surface fol owing distal humeral coronal shear fractures. The lateral extensile approach and fixation with countersunk headless compression screws placed in an anterior-to-posterior fashion are commonly used. We have found a two-incision approach(direct anterior and lateral) that results in less soft tissue dissection and better outcomes than the lateral extensile approach in our experience. Stiffness, pain, articular incongruity, arthritis, and ulnohumeral instability may result if reduction is non-anatomic or if fixation fails. | Shahram S Yari Nathan L Bowers Miguel A Craig Lee M Reichel | 2015 | World Journal of Clinical Cases2015，3，5： | 15 |
| 6 | The wonders of BMP9:From mesenchymal stem cell differentiation,angiogenesis,neurogenesis,tumorigenesis,and metabolism to regenerative medicine显示文摘Although bone morphogenetic proteins(BMPs)initially showed effective induction of ectopic bone growth in muscle,it has since been determined that these proteins,as members of the TGF-b superfamily,play a diverse and critical array of biological roles.These roles include regulating skeletal and bone formation,angiogenesis,and development and homeostasis of multiple organ systems.Disruptions of the members of the TGF-b/BMP superfamily result in severe skeletal and extra-skeletal irregularities,suggesting high therapeutic potential from understanding this family of BMP proteins.Although it was once one of the least characterized BMPs,BMP9 has revealed itself to have the highest osteogenic potential across numerous experiments both in vitro and in vivo,with recent studies suggesting that the exceptional potency of BMP9 may result from unique signaling pathways that differentiate it from other BMPs.The effectiveness of BMP9 in inducing bone formation was recently revealed in promising experiments that demonstrated efficacy in the repair of critical sized cranial defects as well as compatibility with bone-inducing bio-implants,revealing the great translational promise of BMP9.Furthermore,emerging evidence indicates that,besides its osteogenic activity,BMP9 exerts a broad range of biological functions,including stem cell differentiation,angiogenesis,neurogenesis,tumorigenesis,and metabolism.This review aims to summarize our current understanding of BMP9 across biology and the body. | Sami Mostafa Mikhail Pakvasa Elam Coalson Allen Zhu Alex Alverdy Hector Castillo Jiaming Fan Alex Li Yixiao Feng Di Wu Elliott Bishop Scott Du Mia Spezia Alissa Li Ofir Hagag Alison Deng Winny Liu Mingyang Li Sherwin S·Ho Aravind Athiviraham Michael J·Lee Jennifer Moriatis Wolf Guillermo A·Ameer Hue H·Luu Rex C·Haydon Jason Strelzow Kelly Hynes Tong-Chuan He Russell R·Reid | 2019 | Genes & Diseases2019，6，3： | 15 |
| 7 | The Qitai radio telescope显示文摘This study presents a general outline of the Qitai radio telescope(QTT)project.Qitai,the site of the telescope,is a county of Xinjiang Uygur Autonomous Region of China,located in the east Tianshan Mountains at an elevation of about 1800 m.The QTT is a fully steerable,Gregorian-type telescope with a standard parabolic main reflector of 110 m diameter.The QTT has adopted an umbrella support,homology-symmetric lightweight design.The main reflector is active so that the deformation caused by gravity can be corrected.The structural design aims to ultimately allow high-sensitivity observations from 150 MHz up to115 GHz.To satisfy the requirements for early scientific goals,the QTTwill be equipped with ultra-wideband receivers and large field-of-view multi-beam receivers.A multi-function signal-processing system based on RFSo C and GPU processor chips will be developed.These will enable the QTT to operate in pulsar,spectral line,continuum and Very Long Baseline Interferometer(VLBI)observing modes.Electromagnetic compatibility(EMC)and radio frequency interference(RFI)control techniques are adopted throughout the system design.The QTT will form a world-class observational platform for the detection of lowfrequency(nano Hertz)gravitational waves through pulsar timing array(PTA)techniques,pulsar surveys,the discovery of binary black-hole systems,and exploring dark matter and the origin of life in the universe.The QTT will also play an important role in improving the Chinese and international VLBI networks,allowing high-sensitivity and high-resolution observations of the nuclei of distant galaxies and gravitational lensing systems.Deep astrometric observations will also contribute to improving the accuracy of the celestial reference frame.Potentially,the QTT will be able to support future space activities such as planetary exploration in the solar system and to contribute to the search for extraterrestrial intelligence. | Na Wang Qian Xu Jun Ma Zhiyong Liu Qi Liu Hailong Zhang Xin Pei Maozheng Chen Richard N.Manchester Kejia Lee Xingwu Zheng Hans J.Kärcher Wulin Zhao Hongwei Li Dongwei Li Martin Süss Matthias Reichert Zhongyi Zhu Congsi Wang Mingshuai Li Rui Li Ning Li Guljaina Kazezkhan Wenming Yan Gang Wu Lang Cui Ming Zhang Haitao Li | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，8： | 14 |
| 8 | Calcium signaling and T-type calcium channels in cancer cell cycling显示文摘Regulation of intracellular calcium is an important signaling mechanism for cell proliferation in both normal and cancerous cells. In normal epithelial cells, free calcium concentration is essential for cells to enter and accomplish the S phase and the M phase of the cell cycle. In contrast, cancerous cells can pass these phases of the cell cycle with much lower cytoplasmic free calcium concentrations, indicating an alternative mechanism has developed for fulfilling the intracellular calcium requirement for an increased rate of DNA synthesis and mitosis of fast replicating cancerous cells. The detailed mechanism underlying the altered calcium loading pathway remains unclear; however, there is a growing body of evidence that suggests the T-type Ca2+ channel is abnormally expressed in cancerous cells and that blockade of these channels may reduce cell proliferation in addition to inducing apoptosis. Recent studies also show that the expression of T-type Ca2+ channels in breast cancer cells is proliferation state dependent, i.e. the channels are expressed at higher levels during the fast-replication period, and once the cells are in a non-proliferation state, expression of this channel isminimal. Therefore, selectively blocking calcium entry into cancerous cells may be a valuable approach for preventing tumor growth. Since T-type Ca2+ channels are not expressed in epithelial cells, selective T-type Ca2+ channel blockers may be useful in the treatment of certain types of cancers. | James T Taylor Xiang-Bin Zeng Jonathan E Pottle Kevin Lee Alun R Wang Stephenie G Yi lennifer A S Scruggs Suresh S Sikka Ming Li | 2008 | World Journal of Gastroenterology2008，14，32： | 12 |
| 9 | Endosonography in the diagnosis and management of pancreatic cysts显示文摘Rapid advances in radiologic technology and increased cross-sectional imaging have led to a sharp rise in incidental discoveries of pancreatic cystic lesions. These cystic lesions include non-neoplastic cysts with no risk of malignancy, neoplastic non-mucinous serous cystadenomas with little or no risk of malignancy, as well as neoplastic mucinous cysts and solid pseudopapillary neoplasms both with varying riskof malignancy. Accurate diagnosis is imperative as management is guided by symptoms and risk of malignancy. Endoscopic ultrasound(EUS) allows high resolution evaluation of cyst morphology and precise guidance for fine needle aspiration(FNA) of cyst fluid for cytological, chemical and molecular analysis. Initially, clinical evaluation and radiologic imaging, preferably with magnetic resonance imaging of the pancreas and magnetic resonance cholangiopancreatography, are performed. In asymptomatic patients where diagnosis is unclear and malignant risk is indeterminate, EUSFNA should be used to confirm the presence or absence of high-risk features, differentiate mucinous from non-mucinous lesions, and diagnose malignancy. After analyzing the cyst fluid for viscosity, cyst fluid carcinoembryonic antigen, amylase, and cyst wall cytology should be obtained. DNA analysis may add useful information in diagnosing mucinous cysts when the previous studies are indeterminate. New molecular biomarkers are being investigated to improve diagnostic capabilities and management decisions in these challenging cystic lesions. Current guidelines recommend surgical pancreatic resection as the standard of care for symptomatic cysts and those with high-risk features associated with malignancy. EUSguided cyst ablation is a promising minimally invasive, relatively low-risk alternative to both surgery and surveillance. | Vivek Kadiyala Linda S Lee | 2015 | World Journal of Gastrointestinal Endoscopy2015，7，3： | 12 |
| 10 | 婴儿和儿童睾丸卵黄囊瘤33例并临床病理特征分析显示文摘作者收集1918~2014年诊断的发生于5~71个月(平均20.7个月)的男孩睾丸纯卵黄囊瘤33例。除1例外所有患者均接受睾丸切除,18例同时行淋巴结清扫术(全部阴性);21例接受化疗,12例接受放疗。 | Cornejo K M Frazier L Lee R S 魏建国 许春伟 | 2015 | 临床与实验病理学杂志2015，31，9： | 11 |
| 11 | Endoscopic ultrasound-guided techniques for diagnosing pancreatic mass lesions: Can we do better?显示文摘The diagnostic approach to a possible pancreatic mass lesion relies first upon various non-invasive imaging modalities, including computed tomography, ultrasound, and magnetic resonance imaging techniques. Once a suspect lesion has been identified, tissue acquisition for characterization of the lesion is often paramount in developing an individualized therapeutic approach. Given the high prevalence and mortality associated with pancreatic cancer, an ideal approach to diagnosing pancreatic mass lesions would be safe, highly sensitive, and reproducible across various practice settings. Tools, in addition to radiologic imaging, currently employed in the initial evaluation of a patient with a pancreatic mass lesion include serum tumor markers, endoscopic retrograde cholangiopancreatography, and endoscopic ultrasound-guided fine needle aspiration(EUS-FNA). EUS-FNA has grown to become the gold standard in tissue diagnosis of pancreatic lesions. | Andrew C Storm Linda S Lee | 2016 | World Journal of Gastroenterology2016，22，39： | 10 |
| 12 | 柑桔黄龙病的鉴定和柯赫氏定理(英文)显示文摘黄龙(梢)病被认为是世界柑橘生产上的毁灭性病害已超过一个世纪,但该病害的病原学至今还没有清晰地建立起来。根据16S rRNA基因序列分析,一组被称为'Candidatus Liberibacter species'的难培养细菌被认为与黄龙病相关。然而,要确定'Ca.Liberibacter spp.'是黄龙病病原的柯赫氏定理并没有真正完成。令我们担忧的是,近年来有些文献频频指出'Ca.Liberibacter spp.'是黄龙病的病原,其实该细菌与柑橘寄主的直接病理反应还没有完全确定。我们建议,在黄龙病病原学清楚之前,文献报道在这方面需要有准确的阐述。 | Chen J Deng X Civerolo E L Lee R F Jones J B Zhou C Hartung J S Manjunath K L Brlansky R H | 2011 | 植物病理学报2011，41，2： | 10 |
| 13 | 铁限制条件下东海原甲藻分泌铁载体显示文摘在铁限制条件下,进行东海原甲藻分泌铁载体的动态研究。对藻类在富铁与缺铁条件下生长状况、生长过程中分泌铁载体的情况以及海藻接种量对铁载体分泌的影响进行了连续观测,结果表明:东海原甲藻在缺铁条件下生长状况远不如在富铁条件下;随着藻类的生长,分泌铁载体不断增多,达指数生长期时,其分泌量也达到了最大值,之后藻类的生长和铁载体分泌都呈现下降趋势;高接种量东海原甲藻能分泌较多的铁载体,并在较短时间到达峰值。 | 王颜萍 何鹰 郑晓玲 陈发荣 李瑞香 Lee Frank S C 王小如 | 2005 | 生态学报2005，25，10： | 10 |
| 14 | Adaptive and maladaptive expression of the mRNA regulatory protein HuR显示文摘The RNA-binding proteins involved in regulation of mRNA post-transcriptional processing and translation control the fates of thousands of mRNA transcripts and basic cellular processes. The best studied of these, HuR, is well characterized as a mediator of mRNA stability and translation, and more recently, as a factor in nuclear functions such as pre-mRNA splicing. Due to HuR's role in regulating thousands of mRNA transcripts, including those for other RNA-binding proteins, HuR can act as a master regulator of cell survival and proliferation. HuR itself is subject to multiple post-translationa modifications including regulation of its nucleocytoplasmic distribution. However, the mechanisms that govern HuR levels in the cell have only recently begun to be defined. These mechanisms are critical to cell health, as it has become clear in recent years that aberrant expression of HuR can lead alternately to decreased cell viability or to promotion of pathological proliferation and invasiveness. HuR is expressed as alternate mRNAs that vary in their untranslated regions, leading to differences in transcript stability and translatability. Multiple transcription factors and modulators of mRNA stability that regulate HuR mRNA expression have been identified. In addition, translation of HuR is regulated by numerous microRNAs, several of which have been demonstrated to have anti-tumor properties due to their suppression of HuR expression. This review summarizes the current state of knowledge of the factors that regulate HuR expression, along with the circumstances under which these factors contribute to cancer and inflammation. | Suman Govindaraju Beth S Lee | 2013 | World Journal of Biological Chemistry2013，4，4： | 7 |
| 15 | Multiple Antibiotic Resistance and Heavy Metal Resistance Profile of Bacteria Isolated from Giant Freshwater Prawn (Macrobrachium rosenbergii) Hatchery显示文摘In this article, antibiogram and heavy metal resistance profile of bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) hatchery in Malaysia are described. Although giant freshwater prawn was introduced into Malaysia since the 1980s, there was no database information on antibiogram and heavy metal resistance profile of bacteria from giant freshwater prawn (M. rosenbergii) hatchery in Malaysia. Therefore, this study was carried out to determine the effectiveness of antibiotic and heavy metal resistance profile to control bacterial diseases in M. rosenbergii hatchery. The results can provide valuable information for local M. rosenbergii post-larval producer. Antibiotic sensitivity test was carried out by disk-diffusion method against 15 types of antibiotics as follows: oxolinic acid (2 μg), ampicillin (10 μg), erythromycin (15 μg), furazolidone (15 μg), lincomycin (15 μg), amoxicillin (25 μg), colistin sulphate (25 μg), doxycycline (30 μg), florfenicol (30 μg), flumequine (30 μg), nalidixic acid (30 μg), tetracycline (30 μg), oleandomycin (15 μg), fosfomycin (50 μg), and spiramycin (100 μg), whereas heavy metal resistance profile of the present bacterial isolates was determined by 2-fold agar dilution technique. In this study, 5 types of bacteria were successfully isolated; they were Aeromonas spp. (n = 77), Escherichia coli (n = 73), Edwardsiella spp. (n = 62), Salmonella spp. (n = 75), and Vibrio spp. (n = 43). The result showed that furazolidone was the most effective antibiotic to control the bacteria isolated in this study, approximately 89.7% of the bacterial isolates were sensitive to this antibiotic. Multiple antibiotic resistance (MAR) index indicated that the hatchery water source and M. rosenbergii post-larval and sediment tanks were at high-risk exposure to the tested antibiotic. Furthermore, all the tested heavy metals (Cd2+, Cr6+, Hg2+, and Cu2+) failed to inhibit the growth of the bacterial isolates. Therefore, it indicated that the water source of the hatchery is contaminated with both antibiotic residues and heavy metal. | S W Lee M Najiah W Wendy A Zahrol M Nadirah | 2009 | Agricultural Sciences in China2009，8，6： | 7 |
| 16 | C_2H_4-O_2混合气体直接起爆的临界能量显示文摘主要基于化学动力学和Ng模型,对C2H4-O2混合气体的爆轰胞格尺寸进行预测;结合Lee表面积能量模型,预测物质在不同初始压力和化学当量比的条件下,直接起爆引起球面爆轰的临界起爆能量。直接起爆实验主要采用高压电点火提供起爆能量,起爆能量通过放电过程中电流的输出信号确定。结果表明,理论预测与实验值较吻合。首先,通过化学动力学计算得出ZND模型的爆轰参数,利用Ng模型得出爆轰胞格尺寸与ZND诱导区长度之间的比例因数A在不同初始压力与当量比的条件下分别为:A=43.815(1+p1/p0)-0.123 71和A=8.531exp(φ/3.135)+28.644,在此基础上对爆轰胞格尺寸进行定量预测。胞格尺寸的理论预测与实验结果吻合。其次,把爆轰胞格尺寸作为中间特征参数并结合Lee的表面积能量模型,提出可以预测临界起爆能量的定量模型,并得出C2H4-O2混合气体直接起爆的临界起爆能量与初始压力和化学当量比的参量拟合关系分别为Ec=0.332(p1/p0)-2.017和Ec=exp[3.951(φ-1.401)2-1.9]。 | 张博 Lee J H S 白春华 | 2012 | 爆炸与冲击2012，32，2： | 7 |
| 17 | 运动对新发脂肪肝和已存在脂肪肝的影响显示文摘非酒精性脂肪性肝病(NAFLD)是指非饮酒所致的肝脏过多脂肪堆积,往往常见于超重、肥胖或罹患糖尿病、高胆固醇血症或高甘油三酯血症的个体。NAFLD是目前最常见的慢性肝脏疾病之一,影响了30%以上的一般人群,已成为一个严重的公共卫生负担。在全世界范围内,随着肥胖的流行,该综合征的患病率不断增加。当前,尚没有一种药物或手术获批用于治疗NAFLD。一些研究总结称,减重是唯一经证实的治疗NAFLD的有效方案。 | Sung KC Ryu S Lee JY | 2016 | 胃肠病学和肝病学杂志2016，25，12： | 7 |
| 18 | Comparison of non-schistosomal rectosigmoid cancer and schistosomal rectosigmoid cancer显示文摘AIM: To compare the clinicopathological features of patients with non-schistosomal rectosigmoid cancer and schistosomal rectosigmoid cancer.METHODS: All the patients with rectosigmoid carcinoma who underwent laparoscopic radical surgical resection in the Shanghai Minimally Invasive Surgical Center at Ruijin Hospital affiliated to Shanghai JiaoTong University between October 2009 and October2013 were included in this study. Twenty-six cases of colonic schistosomiasis diagnosed through colonoscopy and pathological examinations were collected. Symptoms,endoscopic findings and clinicopathological characteristics were evaluated retrospectively.RESULTS: There were no significant differences between patients with and without schistosomiasis in gender, age, CEA, CA19-9, preoperative biopsy findings or postoperative pathology. Patients with rectosigmoid schistosomiasis had a significantly higher CA-125 level and a larger proportion of these patients were at an early tumor stage(P = 0.003). Various morphological characteristics of schistosomiasis combined with rectosigmoid cancer could be found by colonoscopic examination: 46% were fungating mass polyps, 23%were congestive and ulcerative polyps, 23% were cauliflower-like masses, 8% were annular masses.Only 27% of the patients were diagnosed with rectal carcinoma preoperatively after the biopsy. Computed tomography(CT) scans showed thickened intestinal walls combined with linear and tram-track calcifications in 26 patients.CONCLUSION: Rectosigmoid carcinoma combined with schistosomiasis is associated with higher CA-125 values and early tumor stages. CA-125 and CT scans have a reasonable sensitivity for the accurate diagnosis. | Hao Feng Ai-Guo Lu Xue-Wei Zhao Ding-Pei Han Jing-Kun Zhao Lei Shi Tobias S Schiergens Serene ML Lee Wen-Peng Zhang Wolfgang E Thasler | 2015 | World Journal of Gastroenterology2015，21，23： | 6 |
| 19 | 4-1BB signaling activates glucose and fatty acid metabolism to enhance CD8^(+) T cell proliferation显示文摘4-1BB(CD137)is a strong enhancer of the proliferation of CD8^(+)T cells.Since these cells require increased production of energy and biomass to support their proliferation,we hypothesized that 4-1BB signaling activated glucose and fatty acid metabolism.We found that treatment with agonistic anti-4-1BB mAb promoted the proliferation of CD8^(+)T cells in vitro,increasing their size and granularity.Studies with a glycolysis inhibitor and a fatty acid oxidation inhibitor revealed that CD8^(+)T cell proliferation required both glucose and fatty acid metabolism.Anti-4-1BB treatment increased glucose transporter 1 expression and activated the liver kinase B1(LKB1)-AMP-activated protein kinase(AMPK)-acetyl-CoA carboxylase(ACC)signaling pathway,which may be responsible for activating the metabolism of glucose and fatty acids.We also examined whether blocking glucose or fatty acid metabolism affected cell cycle progression and the anti-apoptotic effect of 4-1BB signaling.The increase of anti-apoptotic factors and cyclins in response to anti-4-1BB treatment was completely prevented by treating CD8^(+)T cells with the fatty acid oxidation inhibitor,etomoxir,but not with the glycolysis inhibitor,2-deoxy-D-glucose.We conclude that anti-4-1BB treatment activates glucose and fatty acid metabolism thus supporting the increased demand for energy and biomass,and that fatty acid metabolism plays a crucial role in enhancing the cell cycle progression of anti-CD3-activated CD8^(+)T cells in vitro and the anti-apoptotic effects of 4-1BB signaling on these cells. | Beom K Choi Do Y Lee Don G Lee Young H Kim Seon-Hee Kim Ho S Oh Chungyong Han Byoung S Kwon | 2017 | Cellular & Molecular Immunology2017，14，9： | 6 |
| 20 | 实施新生儿复苏项目减少中国新生儿出生窒息死亡显示文摘中国作为增速领先的世界经济力量不断向前发展,妇幼健康状况取得了显著的成效。5岁以下儿童死亡率的降低提前实现了千年发展目标,但是新生儿死亡所占比例仍较高。在相对较高的新生儿死亡率背后,存在农村地区的地理环境不利于提供医疗服务、医疗机构的医疗条件存在差异、基层医务人员技术能力较低等因素。为了解决新生儿死亡问题,尤其是减少新生儿出生窒息的发生率和死亡率,在过去十年间,主要的相关各方已经着手在中国实施新生儿复苏项目。这些努力在临床保健方面已经显现成效,而且在未来会进一步发挥作用,从而改善儿童的长期健康结局。 | 薛艳萍 Xu T Niermeyer S Lee H | 2017 | 中国妇幼卫生杂志2017，8，3： | 5 |
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