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| 1 | Development of loop-mediated isothermal amplification for rapid detection of Entamoeba histolytica显示文摘Objective:To develop a loop-mediated isothermal amplification(LAMP) assay for the detection of Entamoeba histolytica(E.histolytica),the causative agent of amebiasis.Methods:The LAMP primer set was designed from E.histolytica hemolysin gene HLY6.Genomic DNA of E.histolytica trophozoites strain HK9 was used to optimize the LAMP mixture and conditions.Amplification of DNA in the LAMP mixture was monitored through visual inspection for turbidity of the LAMP mix as well as addition of fluorescent dye.Results:Positive LAMP reactions turned turbid while negative ones remained clear.Upon addition of a fluorescent dye,all positive reactions turned green while the negative control remained orange under ambient light After elecrophoresis in 1.5% agarose gels,a ladder of multiple bands of different sizes can be oliserved in positive samples while no bands were detected in the negative control.The sensitivity of the assay was found to be S parasites per reaction which corresponds to approximately 1S.8 ng/μL DNA.The specificity of the assay was verified by the absence of amplified products when DNA from other gastrointestinal parasites such as the morphologically similar but non-pathogenic species,Entamoeba dispar. and other diarrhea-causing organisms such as Blastocystis hominis and Escherichia coli were used.Conclusions:The I.AMP assay we have developed enables the detection of E.histolytica with rapidity and ease,therefore rendering it is suitable for laboratory and field diagnosis of amebiasis. | Windell L.Rivera Vanissa A.Ong | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，6： | 2 |
| 2 | 2009年萨摩亚—汤加特大地震触发的两次大震显示文摘特大地震(≥8.0级的地震)一般都含有构造板块之间边界岩体的突然滑动。这种板间的破裂通常出现在俯冲带特大逆冲事件的海沟斜坡区,会产生动态及静态应力变化,从而激活周边的板内余震(Christensen and Ruff,1988;Dmowskaetal,1988;Layetal,1989;Ammonetal,2008)。本文研究的地震序列展示一少见的例子———特大海沟斜坡的一次板内地震触发了广泛的板间断层活动,颠倒了典型的活动模式,从而广泛地扩大了地震和海啸灾害。2009年9月29日,在汤加俯冲带北端的外海沟斜坡发生矩震级8.1的正断层地震事件,该震开始破裂后的2分钟内,发生了总地震矩等于矩震级8.0级的第二个特大地震,它由两次(矩震级均为7.8级)板间下插逆冲大地震组成,导致了周边俯冲带巨型逆断层的破裂。联合的断层作用引发了海啸,局部地区抬升约12m,导致萨摩亚、美属萨摩亚和汤加192人死亡。地震信号的重叠掩盖了这样的事实:相隔约50km的性质不同的断层发生了不同几何形状的破裂,这些被触发的逆冲断层滑动只有通过详细的地震波分析才能揭示出来。在汤加北部俯冲带的大部分区域内,激活了广泛的板间和板内余震活动。 | T.Lay C.J.Ammon H.Kanamori L.Rivera K.D.Koper A.R.Hutko 李晶 | 2011 | 世界地震译丛2011，42，3： | 1 |
| 3 | 使用地球低频的自由振荡约束2004年苏门答腊和2005年尼亚斯地震总体的运动学显示文摘巨大的2004年亚齐?苏门答腊地震是自1960年智利特大地震和1964年阿拉斯加特大地震以来记录的最大地震。地球自由振荡得到很强的激发,并被大量台站记录到,其信噪比极好,即便低频的振型也如此。这些特殊振型非常有意义,因为这些分隔良好的分裂自由振荡的相位携带了有关大地震(Mw>8)震源的运动学信息,尤其是有关长度、持续时间和平均破裂速度的信息。使用单峰剥离技术,我们研究了几个宽带永久台站[地球透镜、地震学联合研究协会(IRIS)和全球地球动力学计划(GGP)]记录的一些地球最低频自由振荡(0S2、0S3、0S4、1S2、0S0和1S0),以恢复各个单峰参数:相位、频率和品质因子。我们使用这些参数约束了2004年12月26日苏门答腊地震和2005年3月28日尼亚斯地震的震源时空展布。我们主要使用地震仪和超导重力仪的垂直分量数据,因为这些数据的噪声比水平地震数据低,但我们还需指出,对2004年地震也可使用水平分量(1S2)。根据本文给出的初始相位测量结果,我们得到2004年地震断层长度约为1250km,持续时间约为550s。对于2005年地震,我们测定所倾向的模型是,南段约40s之后破裂,但破裂的双向性质及其空间尺度使我们不能适当地约束其空间范围。 | S.Lambotte L.Rivera J.Hinderer 吕春来 | 2008 | 世界地震译丛2008，，4： | 0 |
| 4 | 由地球极慢自由振荡相位得到的2004年亚齐-苏门答腊地震的破裂长度和持续时间显示文摘亚齐-苏门答腊2004年地震很强地激发了地球自由振荡。完全分裂的振幅提供了地震震源的有用信息。特别是分裂的单峰相位约束了持续时间、破裂长度和平均破裂速度。为约束震源的时空有限性,我们分析了地球的某些极慢自由振荡(0S2,0S3,0S0和1S0)的初始相位。我们使用了几个世界范围的地球物理台网的宽频带地震仪垂直记录和超导重力仪数据。估计的该地震的破裂长度约为1220km,震源持续时间约为500s,平均破裂速度为2·4km/s。 | S.Lambotte L.Rivera J.Hinderer 万永革 | 2007 | 世界地震译丛2007，38，4： | 0 |
| 5 | Antibacterial activities of ethanol extracts of Philippine medicinal plants against multidrug-resistant bacteria显示文摘Objective: To investigate the antibacterial activities of crude ethanol extracts of 12 Philippine medicinal plants.Methods: Crude ethanol extracts from 12 Philippine medicinal plants were evaluated for their antibacterial activity against methicillin-resistant Staphylococcus aureus, vancomycinresistant Enterococcus, extended spectrum β-lactamase-producing, carbapenem-resistant Enterobacteriaceae and metallo-β-lactamase-producing Pseudomonas aeruginosa and Acinetobacter baumannii. Results: The leaf extracts of Psidium guajava, Phyllanthus niruri, Ehretia microphylla and Piper betle(P. betle) showed antibacterial activity against the Gram-positive methicillinresistant Staphylococcus aureus and vancomycin-resistant Enterococcus. P. betle showed the highest antibacterial activity for these bacteria in the disk diffusion(16-33 mm inhibition diameter), minimum inhibitory concentration(19-156 μg/m L) and minimum bactericidal concentration(312 μg/m L) assays. P. betle leaf extracts only showed remarkable antibacterial activity for all the Gram-negative multidrug-resistant bacteria(extended spectrum β-lactamaseproducing, carbapenem-resistant Enterobacteriaceae and metallo-β-lactamase-producing) in the disk diffusion(17-21 mm inhibition diameter), minimum inhibitory concentration(312-625 μg/m L) and minimum bactericidal concentration(312-625 μg/m L) assays. Conclusions: P. betle had the greatest potential value against both Gram-negative and Grampositive multidrug-resistant bacteria. Favorable antagonistic activities were also exhibited by the ethanol extracts of Psidium guajava, Phyllanthus niruri and Ehretia microphylla. | Demetrio L.Valle Jr. Jeannie I.Andrade Juliana Janet M.Puzon Esperanza C.Cabrera Windell L.Rivera | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，7： | 0 |
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