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| 1 | Synaptic localization of P2X<sub>7</sub> receptors in the rat retina显示文摘 | TheresaPuthussery Erica L.Fletcher | 2004 | J Comp Neurol2004，，1： | 1 |
| 2 | Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment Epithelium over Time显示文摘Human pluripotent stem cell(h PSC)-derived progenies are immature versions of cells,presenting a potential limitation to the accurate modelling of diseases associated with maturity or age.Hence,it is important to characterise how closely cells used in culture resemble their native counterparts.In order to select appropriate time points of retinal pigment epithelium(RPE)cultures that reflect native counterparts,we characterised the transcriptomic profiles of the h PSC-derived RPE cells from 1-and 12-month cultures.We differentiated the human embryonic stem cell line H9 into RPE cells,performed single-cell RNA-sequencing of a total of 16,576 cells to assess themolecular changes of the RPE cells across these two culture time points.Our results indicate the stability of the RPE transcriptomic signature,with no evidence of an epithelial–mesenchymal transition,and with the maturing populations of the RPE observed with time in culture.Assessment of Gene Ontology pathways revealed that as the cultures age,RPE cells upregulate expression of genes involved in metal binding and antioxidant functions.This might reflect an increased ability to handle oxidative stress as cells mature.Comparison with native human RPE data confirms a maturing transcriptional profile of RPE cells in culture.These results suggest that long-term in vitro culture of RPE cells allows the modelling of specific phenotypes observed in native mature tissues.Our work highlights the transcriptional landscape of h PSC-derived RPE cells as they age in culture,which provides a reference for native and patient samples to be benchmarked against. | Grace E.Lidgerwood Anne Senabouth Casey J.A.Smith-Anttila Vikkitharan Gnanasambandapillai Dominik C.Kaczorowski Daniela Amann-Zalcenstein Erica L.Fletcher Shalin H.Naik Alex W.Hewitt Joseph E.Powell Alice Pebay | 2021 | Genomics, Proteomics & Bioinformatics2021，19，2： | 1 |
| 3 | Retinitis pigmentosa: understanding the clinical presentation, mechanisms and treatment options显示文摘 | MichaelKalloniatis Erica L.Fletcher | 2009 | Clinical and Experimental Optometry2009，，2： | 1 |
| 4 | 抗冻肽对鱼耐冻力影响的研究显示文摘通常认为血浆抗冻蛋白对许多海水硬骨鱼在冰冻海水中的生存能力有直接的作用,然而没有直接的实验证据证明这一推断是否正确。本研究中,美洲拟鲽显示出耐冻力与血浆抗冻蛋白同样呈现季节性变化。当把美洲拟鲽的抗冻蛋白注射到虹鳟(这种鱼本身不含有抗冻蛋白)体内时,能增强虹鳟的耐冻力,并且与其血浆中的抗冻蛋白含量直接相关。这些研究证明抗冻蛋白与许多硬骨鱼在低于它们血液冰点温度的冰冻海水中生存能力有直接关系,而且鱼体中不需要特殊抗冻蛋白受体来完成这一功能。 | GARTH L.FLETCHER 简纪常 | 1994 | 国外水产1994，，2： | 0 |
| 5 | Revealing climatic impacts on the temporal and spatial variation in vegetation activity across China:Sensitivity and contribution显示文摘Relative weakness in sensitivity and contribution integration,especially lack of specific indicators at spatial and temporal scales,may hinder the formation of systematic knowledge for climate change impact on vegetation activity.Considering the interannual variability and periods difference,we investigated the ecosystem sensitivity to hydrothermal conditions and the contribution of individual climatic factors to vegetation activity on spatiotemporal scales during the period of 1980s—2013 in China.We ran simulations of the Common Land Model(CoLM)to obtain the photosynthetic rate,and used the satellite-derived Normalized Difference Vegetation Index(NDVI),to which we applied a temporal sensitivity analysis,the slope change ratio of accumulative quantity(SCRAQ),and geographical detectors.We show that the discrepancy between warming and precipitation variation contributed to vegetation activity was more notable than the ecosystem sensitivity to hydrothermal conditions,regardless of which temporal and spatial scale was considered.The sensitivity of vegetation activity to hydrothermal variation in most parts of northeastern and western China was higher than elsewhere,and the contribution of warming to vegetation dynamics was relatively high in the Inner Mongolian Plateau and Tibetan Plateau(>60%),where the contribution of warming to the vegetation distribution was also greater than elsewhere in China.Conversely,the contribution of precipitation variation to vegetation dynamics was comparatively greater in northwestern China and subtropical and tropical regions(>55%).The spatiotemporal contribution of precipitation variation to vegetation activity differed between regions,especially on the Tibetan Plateau and in subtropical and tropical regions.Moreover,both the temporal and spatial sensitivities to climate change were weak in the subtropical and tropical regions,where the contribution of climatic factors to vegetation distribution was also small.Overall,temperature sensitivity and its contribution to vegetation activity were positively associated in most regions.However,the regions where precipitation contributed substantially to vegetation activity were not necessarily also highly sensitive to changes in precipitation within the range of variation explored. | JIAO Ke-Wei GAO Jiang-Bo LIU Zhi-Hua WU Shao-Hong Tamara L.FLETCHER | 2021 | Advances in Climate Change Research2021，12，3： | 0 |
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