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| 1 | Blockade of high mobility group box-1 protein attenuates experimental severe acute pancreatitis显示文摘AIM: To examine the effects of anti-high mobility group box 1 (HMGB1) neutralizing antibody in experimental se-vere acute pancreatitis (SAP).METHODS: SAP was induced by creating closed duode-nal loop in C3H/HeN mice. SAP was induced immediately after intraperitoneal injection of anti-HMGB1 neutralizing antibody (200 μg). Severity of pancreatitis, organ injury (liver, kidney and lung), and bacterial translocation to pancreas was examined 12 h after induction of SAP.RESULTS: Anti-HMGB1 neutralizing antibody significant-ly improved the elevation of the serum amylase level and the histological alterations of pancreas and lung in SAP. Anti-HMGB1 antibody also significantly ameliorated the elevations of serum alanine aminotransferase and cre-atinine in SAP. However, anti-HMGB1 antibody worsened the bacterial translocation to pancreas.CONCLUSION: Blockade of HMGB1 attenuated the development of SAP and associated organ dysfunction, suggesting that HMGB1 may act as a key mediator for inflammatory response and organ injury in SAP. | Hidehiro Sawa Takashi Ueda Yoshifumi Takeyama Takeo Yasuda Makoto Shinzeki Takahiro Nakajima Yoshikazu Kuroda | 2006 | World Journal of Gastroenterology2006，12，47： | 45 |
| 2 | Apoptosis and non-alcoholic fatty liver diseases显示文摘The number of patients with nonalcoholic fatty liver diseases(NAFLD) including nonalcoholic steatohepatitis(NASH), has been increasing. NASH causes cirrhosis and hepatocellular carcinoma(HCC) and is one of the most serious health problems in the world. The mechanism through which NASH progresses is still largely unknown. Activation of caspases, Bcl-2 family proteins, and c-Jun N-terminal kinase-induced hepatocyte apoptosis plays a role in the activation of NAFLD/NASH. Apoptotic hepatocytes stimulate immune cells and hepatic stellate cells toward the progression of fibrosis in the liver through the production of inflammasomes and cytokines. Abnormalities in glucose and lipid metabolism as well as microbiota accelerate these processes. The production of reactive oxygen species, oxidative stress, and endoplasmic reticulum stress is also involved. Cell death, including apoptosis, seems very important in the progression of NAFLD and NASH. Recently, inhibitors of apoptosis have been developed as drugs for the treatment of NASH and may prevent cirrhosis and HCC. Increased hepatocyte apoptosis may distinguish NASH from NAFLD, and the improvement of apoptosis could play a role in controlling the development of NASH. In this review, the association between apoptosis and NAFLD/NASH are discussed. This review could provide their knowledge, which plays a role in seeing the patients with NAFLD/NASH in daily clinical practice. | Tatsuo Kanda Shunichi Matsuoka Motomi Yamazaki Toshikatsu Shibata Kazushige Nirei Hiroshi Takahashi Tomohiro Kaneko Mariko Fujisawa Teruhisa Higuchi Hitomi Nakamura Naoki Matsumoto Hiroaki Yamagami Masahiro Ogawa Hiroo Imazu Kazumichi Kuroda Mitsuhiko Moriyama | 2018 | World Journal of Gastroenterology2018，24，25： | 32 |
| 3 | Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism显示文摘植物 metabolomics 作为一个强大的工具发展了检验基因功能并且获得更深的卓见进植物房间的生理学。在这研究,我们屏蔽了 Arabidopsis 线 overexpressing 米饭全身(FL ) 用煤气的 chromatographytime-of-flight 团 spectrometry (GCTOF/MS ) 的 cDNAs (米饭狐狸 Arabidopsis 线) 基于技术识别引起了新陈代谢的变化的米饭基因。这个屏蔽系统允许显示出改变的代谢物侧面的候选人线的快、可靠的鉴定。我们执行了怀有 Arabidopsis 的侧面的机关边界(高球) 领域(LBD )/Asymmetric 象 Leaves2 一样(ASL ) 基因的米饭 ortholog 的 FL cDNA 的一根米饭狐狸 Arabidopsis 线的 metabolomic 和 transcriptomic 分析, At-LBD37/ASL39。调查米饭狐狸 Arabidopsis 线在与氮新陈代谢有关的代谢物的层次显示出突出的变化。象从 Arabidopsis At-LBD37/ASL39-overexpressor 植物的代谢物分析的结果一样的 transcriptomic 数据与这些调查结果一致。而且, Os-LBD37/ASL39-overexpressing 米饭植物的 metabolomic 和 transcriptomic 分析显示 Os-LBD37/ASL39 在米饭与与氮新陈代谢有关的过程被联系。因此,一个基于 metabolomics 的屏蔽方法的联合和一条 gain-of-function 途径为在 Arabidopsis 和米饭的新奇基因的快速的描述是有用的。 | Doris Albinsky Miyako Kusano Mieko Higuchi Naomi Hayashi Makoto Kobayashi Atsushi Fukushima Masaki Mori Takanari Ichikawa Keiko Matsui Hirofumi Kuroda Yoko Horii Yuko Tsumoto Hitoshi Sakakibara Hirohiko Hirochika Minami Matsui Kazuki Saito | 2010 | Molecular Plant2010，3，1： | 15 |
| 4 | 城市基础设施水平如何影响居民工资收入:溢价还是折价显示文摘已有文献多从宏观视角上考察基础设施的经济增长效应,文章则从微观视角出发,探讨城市基础设施影响居民收入的微观机制并对其进行实证检验。文章将'中国家庭收入调查(CHIP)'数据与城市特征数据相结合,将城市土地出让收入变动作为城市基础设施的工具变量,通过2SLS方法估计了基础设施水平对居民工资收入的影响。结果表明:基础设施水平对城市居民工资收入具有溢价作用。城市居民名义工资在基础设施水平高的城市更高。基础设施水平对工资收入的溢价作用受居民教育异质性的影响,居民教育水平越高,基础设施水平的溢价作用越大。 | 郑丹 Tatsuaki Kuroda | 2017 | 南方经济2017，46，1： | 15 |
| 5 | Non-invasive determination of hepatic steatosis by acoustic structure quantification from ultrasound echo amplitude显示文摘AIM:To use leptin-deficient(ob/ob) mice with demonstrated differences in steatosis levels to test a new diagnostic method using the acoustical structure quantification(ASQ) mode and the associated analytical parameter,'focal disturbance ratio'(FD-ratio).METHODS:Nine ob/ob mice,at 5,8,and 12 wk of age(n = 3 in each age group),were used as models for hepatic steatosis.Echo signals obtained from ultrasonography in the mice were analyzed by ASQ,which uses a statistical analysis of echo amplitude to estimate inhomogeneity in the diagnostic region.FD-ratio,as calculated from this analysis,was the focus of the present study.FD-ratio and fat droplet areas and sizes were compared between age groups.RESULTS:No fibrosis or inflammation was observed in any of the groups.The fat droplet area significantly(P < 0.01) increased with age from 1.25% ± 0.28% at 5 wk to 31.07% ± 0.48% at 8 wk to 51.69% ± 3.19% at 12 wk.The median fat droplet size also significantly(P < 0.01) increased with age,from 1.33(0.55-10.52) m at 5 wk,2.82(0.61-44.13) m at 8 wk and 6.34(0.66-81.83) m at 12 wk.The mean FD-ratio was 0.42 ± 0.11 at 5 wk,0.11 ± 0.05 at 8 wk,and 0.03 ± 0.02 at 12 wk.The FD-ratio was significantly lower at 12 wk than at 5 wk and 8 wk(P < 0.01).A significant negative correlation was observed between the FD-ratio and either the fat droplet area(r =-0.7211,P = 0.0017) or fat droplet size(r =-0.9811,P = 0.0052).CONCLUSION:This tool for statistical analysis of signals from ultrasonography using the FD-ratio can be used to accurately quantify fat in vivo in an animal model of hepatic steatosis,and may serve as a quantitative biomarker of hepatic steatosis. | Hidekatsu Kuroda Keisuke Kakisaka Naohisa Kamiyama Takayoshi Oikawa Mio Onodera Kei Sawara Kanta Oikawa Ryujin Endo Yasuhiro Takikawa Kazuyuki Suzuki | 2012 | World Journal of Gastroenterology2012，18，29： | 15 |
| 6 | Visualizing the hepatic vascular architecture using superb microvascular imaging in patients with hepatitis C virus: A novel technique显示文摘AIM: To identify the hepatic vascular architecture of patients with hepatitis C virus(HCV) using superb microvascular imaging(SMI) and investigate the use of SMI in the evaluation of liver fibrosis.METHODS: SMI was performed in 100 HCV patients. SMI images were classified into five types according to the vascular pattern, and these patterns were compared with the fibrosis stage. Moreover, the images were analyzed to examine vascularity by integrating the number of SMI signals in the region of interest ROI [number of vascular trees(VT)]. The number of VT, fibrosis stage, serum parameters of liver function, and CD34 expression were investigated.RESULTS: There was a significant difference between SMI distribution pattern and fibrosis stage(P < 0.001). The mean VT values in each of the fibrosis stages were as follows: 26.69 ± 7.08 in F0, 27.72 ± 9.32 in F1, 36.74 ± 9.23 in F2, 37.36 ± 5.32 in F3, and 58.14 ± 14.08 in F4. The VT showed excellent diagnostic ability for F4 [area under the receiver operator characteristic(AUROC): 0.911]. The VT was significantly correlated with the CD34 labeling index(r = 0.617, P < 0.0001).CONCLUSION: SMI permitted the detailed delineation of the vascular architecture in chronic liver disease. SMI appears to be a reliable tool for noninvasively detecting significant fibrosis or cirrhosis in HCV patients. | Hidekatsu Kuroda Tamami Abe Keisuke Kakisaka Yudai Fujiwara Yuichi Yoshida Akio Miyasaka Kazuyuki Ishida Hideaki Ishida Tamotsu Sugai Yasuhiro Takikawa | 2016 | World Journal of Gastroenterology2016，22，26： | 12 |
| 7 | Molecular mechanisms of triggering,amplifying and targeting RANK signaling in osteoclasts显示文摘Osteoclast differentiation depends on receptor activator of nuclear factor-κB(RANK) signaling,which can be divided into triggering,amplifying and targeting phases based on how active the master regulator nuclear factor of activated T-cells cytoplasmic 1(NFATc1) is. The triggering phase is characterized by immediateearly RANK signaling induced by RANK ligand(RANKL) stimulation mediated by three adaptor proteins,tumor necrosis factor receptor-associated factor 6,Grb-2-associated binder-2 and phospholipase C(PLC)γ2,leading to activation of IκB kinase,mitogen-activated protein kinases and the transcription factors nuclear factor(NF)-κB and activator protein-1(AP-1). Mice lacking NF-κB p50/p52 or the AP-1 subunit c-Fos(encoded by Fos) exhibit severe osteopetrosis due to a differentiation block in the osteoclast lineage. The amplification phase occurs about 24 h later in a RANKLinduced osteoclastogenic culture when Ca2+ oscillation starts and the transcription factor NFATc1 is abundantly produced. In addition to Ca2+ oscillation-dependent nuclear translocation and transcriptional auto-induction of NFATc1,a Ca2+ oscillation-independent,osteoblastdependent mechanism stabilizes NFATc1 protein in dif-ferentiating osteoclasts. Osteoclast precursors lacking PLCγ2,inositol-1,4,5-trisphosphate receptors,regulator of G-protein signaling 10,or NFATc1 show an impaired transition from the triggering to amplifying phases. The final targeting phase is mediated by activation of numerous NFATc1 target genes responsible for cell-cell fusion and regulation of bone-resorptive function. This review focuses on molecular mechanisms for each of the three phases of RANK signaling during osteoclast differentiation. | Yukiko Kuroda Koichi Matsuo | 2012 | World Journal of Orthopedics2012，3，11： | 10 |
| 8 | Consensus of primary care in acute pancreatitis in Japan显示文摘在日本的尖锐胰腺炎的发生正在增加并且每百万张人口从 187 ~ 347 个盒子。盒子命运是 0.2% 为对温和中等,并且 9.0% 为在在 2003 的日本的严重尖锐胰腺炎。在日本的胰腺炎的专家做了与尖锐胰腺炎在病人的早管理集中于实际方面的这个文件。尖锐胰腺炎和严厉层化的正确诊断应该为尖锐胰腺炎的诊断用标准在所有病人被做并且多,因素得分系统尽早由胰的难处理的疾病的研究委员会求婚了。与尖锐胰腺炎诊断的所有病人应该在医院里被管理。血压监视,脉搏和呼吸率,体温,时时尿的体积,和血氧饱和水平在如此的病人的管理是必要的。早精力旺盛的静脉内的水和具有最前的重要性稳定循环动力学。有鸦片剂的足够的疼痛地势也是重要的。在严重尖锐胰腺炎,在一个早阶段的抗菌素的预防静脉内的管理被推荐。一旦尖锐胰腺炎的诊断被证实,朊酶禁止者的管理应该被开始。如果没有肠塞痛并且胃肠的流血的清楚的症状,从早舞台用非肠道的营养喂的肠内的联合被推荐。有严重尖锐胰腺炎的病人应该尽早被转移到 ICU 执行象朊酶的连续地区性的动脉的注入那样的特殊措施禁止者和抗菌素,和连续牙齿过敏过滤。日本政府为难处理的疾病作为关于措施的研究的工程之一为严重尖锐胰腺炎盖住医疗保健开销。 | Makoto Otsuki Masahiko Hirota Shinju Arata Masaru Koizumi Shigeyuki Kawa Terumi Kamisawa Kazunori Takeda Toshihiko Mayumi Motoji Kitagawa Tetsuhide Ito Kazuo Inui Tooru Shimosegawa Shigeki Tanaka Keisho Kataoka Hiromitsu Saisho Kazuichi Okazaki Yosikazu Kuroda Norio Sawabu Yoshifumi Takeyama | 2006 | World Journal of Gastroenterology2006，12，21： | 9 |
| 9 | A minimally invasive multiple percutaneous drainage technique for acute necrotizing pancreatitis显示文摘BACKGROUND: In approximately 20% of patients, necrotizing pancreatitis is complicated with severe acute pancreatitis, with high morbidity and mortality rates. Minimally invasive step-up approach is both safe and effective, but sometimes requires multiple access sites.METHODS: A 62-year-old woman was admitted with diabetic ketoacidosis, and initial computed tomography(CT) revealed no evidence of acute pancreatitis. She was clinically improved with insulin therapy, fl uid administration, and electrolyte replacement. However, on the 14 th day of admission, she developed a high-grade fever, and CT demonstrated evidence of acute necrotizing pancreatitis with a large collection of peripancreatic fl uid. Percutaneous transgastric drainage was performed and a 14 French gauge(Fr) pigtail catheter was placed 1 week later, which drained copious pus. Because of persistent high-grade fever and poor clinical improvement, multiple 8 and 10 Fr pigtail catheters were placed via the initial drainage route, allowing the safe and effective drainage of the extensive necrotic tissue that was occupying the bilateral anterior pararenal space.RESULTS: After drainage, the patient recovered well and the last catheter was removed on day 123 of admission.CONCLUSIONS: Multiple percutaneous drainage requires both careful judgment and specialist skills. The perforation of the colon and small bowel as well as the injury of the kidney and major vessels can occur. The current technique appears to be safe and minimally invasive compared with other drainage methods in patients with extended, infected necrotic pancreatic pseudocysts. | Takero Terayama Toru Hifumi Nobuaki Kiriu Hiroshi Kato Yuichi Koido Yoshiaki Ichinose Kohei Morimoto Kuroda Yasuhiro | 2014 | World Journal of Emergency Medicine2014，5，4： | 7 |
| 10 | Chloroplast DNA variation and phylogeography of Ligularia tongolensis (Asteraceae), a species endemic to the Hengduan Mountains region of China显示文摘In this research, we aimed to study the genetic variation and phylogeographic pattern of Ligularia tongolensis, a perennial herb endemic to the Hengduan Mountains region of China. We sequenced two chloroplast DNA (cpDNA) intergenic spacers (trnQ-5 rps16, trnL-rpl32) in 140 individuals from 14 populations of three groups (Jinshajiang vs. Yalongjiang vs. Wumeng) within this species range. High levels of haplotype diversity (Hd = 0.814) and total genetic diversity (Ht = 0.862) were detected at the species level, based on a total of 12 haplotypes identified. Low levels of intrapopulation diversity (Hs = 0.349), high levels of genetic divergence (Gst = 0.595, Nst = 0.614, Fst = 0.597), and the absence of isolation by distance tests were also found in L. tongolensis. Furthermore, H2 and H5, the dominant haplotypes that located at internal nodes and deviated from extinct ancestral haplotype in the network, were found to be shared between Jinshajiang and Yalongjiang groups. These results indicate that past fragmentation may be the important factor responsible for the present phylogeographical pattern of L. tongolensis. Meanwhile, the locations occupied by each group might have served as independent refugia for L. tongolensis during the Quaternary glaciation. Unimodal mismatch distribution and star-like genealogies indicated this species underwent past demographic expansion events, with expansion ages of 274 ka BP. | Jin-Feng WANG Yue-Zhi PAN Xun GONG Yu-Chung CHIANG Chiaki KURODA | 2011 | Journal of Systematics and Evolution2011，49，2： | 7 |
| 11 | Molecular pathogenesis of sporadiccolorectal cancers显示文摘Colorectal cancer(CRC)results from the progressive accumulation of genetic and epigenetic alterations that lead to the transformation of normal colonic mucosa to adenocarcinoma.Approximately 75%of CRCs are sporadic and occur in people without genetic predisposition or family history of CRC.During the past two decades,sporadic CRCs were classified into three major groups according to frequently altered/mutated genes.These genes have been identified by linkage analyses of cancer-prone families and by individual mutation analyses of candidate genes selected on the basis of functional data.In the first half of this review,we describe the genetic pathways of sporadic CRCs and their clinicopathologic features.Recently,large-scale genome analyses have detected many infrequently mutated genes as well as a small number of frequently mutated genes.These infrequently mutated genes are likely described in a limited number of pathways.Gene-oriented models of CRC progression are being replaced by pathway-oriented models.In the second half of this review,we summarize the present knowledge of this research field and discuss its prospects. | Hidetsugu Yamagishi Hajime Kuroda Yasuo Imai Hideyuki Hiraishi | 2016 | Chinese Journal of Cancer2016，35，1： | 7 |
| 12 | Transcatheter arterial chemoembolization with a fine-powder formulation of cisplatin for hepatocellular carcinoma显示文摘AIM:To evaluate the efficacy of transcatheter arterial chemoembolization(TACE) using a suspension of a fine-powder formulation of cisplatin(DDPH) for hepatocellular carcinoma(HCC).METHODS:The study population was comprised of 164 patients who were treated by TACE alone.Of these patients,76 underwent TACE using a suspension of DDPH in lipiodol(LPD)(DDPH group),and the remaining 88 underwent TACE with an emulsion of doxorubicin(ADM) with LPD(ADM group).We compared the DDPH group with the ADM group in terms of the objective early response rate,progression free survival(PFS) and overall survival(OS).RESULTS:The objective early response rate in the DDPH group was significantly higher than that in the ADM group(54% vs 24%,P < 0.001).The PFS rate in the DDPH group was also significantly higher than that in the ADM group(P < 0.001).Moreover,the OS in the DDPH group was significantly longer than that in the ADM group(P = 0.002).Although the incidence rate of nausea or vomiting in the DDPH group was higher than that in the ADM group,the ADM group showed a higher incidence rate of the adverse events of hepatic arterial damage and leucopenia.No other serious complications were observed in either group.CONCLUSION:We conclude that TACE using a suspension of DDPH in LPD could be a useful treatment for HCC. | Kazuhiro Kasai Akira Ushio Kei Sawara Yasuhiro Miyamoto Yukiho Kasai Kanta Oikawa Hidekatsu Kuroda Yasuhiro Takikawa Kazuyuki Suzuki | 2010 | World Journal of Gastroenterology2010，16，27： | 6 |
| 13 | Invasive group B streptococcal infection in a patient with post splenectomy for hypersplenism secondary to liver cirrhosis and portal hypertension显示文摘BACKGROUND:Splenectomy in patients with liver cirrhosis(LC)is expected to become more common owing to its efficacy on portal hemodynamics.In this report we describe an alarming case of group B streptococcus(GBS)infection after splenectomy in a patient with LC.METHODS:A 72-year-old woman with a history of LC was admitted to our emergency department because of respiratory failure.The patient had received left lateral segmentectomy of the liver and splenectomy three months before admission.Pulmonary examination revealed significant wheezing during inspiration and expiration,but no crackles and stridor.Chest radiography and CT showed no infiltrates.A presumptive diagnosis of bronchial asthma caused by upper respiratory infection was made.Four days after admission,GBS infection was confirmed by blood culture and penicillin G was administered.Antibiotics were given intravenously for a total of 12 days.RESULTS:The patient was discharged on the 12th day after admission.CONCLUSIONS:Although efficacy of splenectomy in patients with LC has been reported,immune status should be evaluated for a longer period.Patients who have undergone splenectomy are highly susceptible to bacteria;moreover,LC itself is an independent risk factor for mortality in patients with sepsis.Since prophylaxis against GBS has not been established,immediate action should be taken.Emergency physicians should be aware of invasive GBS infection in the context of the critical risk factors related to splenectomy and LC,particularly the expected increase of splenectomy performed in LC patients. | Tomoya Okazaki Toru Hifumi Arisa Manabe Hikari Matsumura Satoshi Egawa Hideyuki Hamaya Nastuyo Shinohara Koshiro Takano Hajime Shishido Yuko Abe Kenya Kawakita Masanobu Hagiike Yasuhiro Kuroda | 2016 | World Journal of Emergency Medicine2016，7，1： | 6 |
| 14 | Colon perforation due to antigenemia-negative cytomegalovirus gastroenteritis after liver transplantation: A case report and review of literature显示文摘BACKGROUND Cytomegalovirus(CMV) remains a critical complication after solid-organ transplantation. The CMV antigenemia(AG) test is useful for monitoring CMV infection. Although the AG-positivity rate in CMV gastroenteritis is known to be low at onset, almost all cases become positive during the disease course. We treated a patient with transverse colon perforation due to AG-negative CMV gastroenteritis, following a living donor liver transplantation(LDLT).CASE SUMMARY The patient was a 52-year-old woman with decompensated liver cirrhosis as a result of autoimmune hepatitis who underwent a blood-type compatible LDLT with her second son as the donor. On day 20 after surgery, upper and lower gastrointestinal endoscopy(GE) revealed multiple gastric ulcers and transverse colon ulcers. The biopsy tissue immunostaining confirmed a diagnosis of CMV gastroenteritis. On day 28 after surgery, an abdominal computed tomography revealed transverse colon perforation, and simple lavage and drainage were performed along with an urgent ileostomy. Although the repeated remission and aggravation of CMV gastroenteritis and acute cellular rejection made the control of immunosuppression difficult, the upper GE eventually revealed an improvement in the gastric ulcers, and the biopsy samples were negative for CMV. The CMV-AG test remained negative, therefore, we had to evaluate the status of the CMV infection on the basis of the clinical symptoms and GE.CONCLUSION This case report suggests a monitoring method that could be useful for AGnegative CMV gastroenteritis after a solid-organ transplantation. | Takahiro Yokose Hideaki Obara Masahiro Shinoda Yutaka Nakano Minoru Kitago Hiroshi Yagi Yuta Abe Yohei Yamada Kentaro Matsubara Go Oshima Shutaro Hori Sho Ibuki Hisanobu Higashi Yuki Masuda Masanori Hayashi Miho Kawaida Takehiko Mori Takumi Fujimura Ken Hoshino Kaori Kameyama Tatsuo Kuroda Yuko Kitagawa | 2019 | World Journal of Gastroenterology2019，25，15： | 5 |
| 15 | A hybrid multi-objective optimization method considering optimization problems in power distribution systems显示文摘Various kinds of new engineering technologies have been studied to realize the low-carbon and sustainable power supply systems all over the world.In actual implementation of these technologies,mostly,there are multiple objectives with trade off relationships among each other,and also various constraints in the achievement of these objectives.Therefore,it should be essential to solve multiobjective optimization problems effectively in the applications of these new technologies in power systems.This paper proposes an improved method to realize multiobjective optimization for critical challenges in advanced power systems.To realize that,in an optimal dispersed generation installation problem,that is,one of effective measures for low-carbon power systems,various optimization methods and their combination methods are evaluated and a hybrid method for evolutionary algorithms was developed.The method can provide improved results compared with other state-of-the-art multi-objective optimization methods. | Ken KURODA Hideki MAGORI Tomiyasu ICHIMURA Ryuichi YOKOYAMA | 2015 | Journal of Modern Power Systems and Clean Energy2015，3，1： | 5 |
| 16 | 东俄洛橐吾遗传变异与分化的ISSR分析显示文摘应用ISSR标记对东俄洛橐吾(Ligularia tongolensis)的遗传多样性进行了研究。从100个引物中筛选出8个用于正式扩增。在所研究的8个居群共150个个体中检测到148个多态位点。在居群水平上,多态位点百分率(PPB)为50.45%,Nei′s基因多样性指数(H)和Shannon信息指数(I)分别为0.1595和0.2440。在物种水平上,多态位点百分率(PPB)为88.10%,Nei′s基因多样性指数(H)和Shannon信息指数(I)分别为0.2811和0.4279。居群间的遗传分化系数(Gst)达0.4355。研究结果表明东俄洛橐吾的遗传多样性水平很高,居群间遗传分化较大。这与其多样化的生态环境是有必然联系的。因适应其多样化的生态环境而形成了遗传多样性;且因其生态环境的不连续性阻碍了居群间的基因交流而产生了遗传分化,即东俄洛橐吾高水平的遗传多样性和遗传分化是适应其分布区多样化生态环境的结果。 | 王金凤 Chiaki kuroda 龚洵 | 2007 | 云南植物研究2007，29，5： | 5 |
| 17 | Natural hybridization and introgression in sympatric Ligularia species(Asteraceae,Senecioneae)显示文摘The difficulty in clarifying species of genus Ligularia Cass.has been attributed to rapid and continuous allopatric speciation in small and isolated populations,combined with interspecific diploid hybridization in the Qinghai-Tibetan Plateau and adjacent areas.However,no concrete example has been reported to prove this hypothesis.We studied a natural mixed population of six species of Ligularia in which some individuals were morphologically intermediate between L.subspicata and L.nelumbifolia.Based on DNA sequences(trnC-F,trnL-rpL32,trnQ5 rps16,trnK-rps16,and internal transcribed spacer) and inter-simple sequence repeat data,we concluded that putative hybrids are primarily products of hybridization between L.nelumbifolia and L.subspicata.The other four species or additional,unknown species may also be involved in hybridization.This hybridization is bidirectional but asymmetrical.Hybrid individuals were mostly the first generation,but F2 and later-generation hybrids were also present.Moreover,the backcrossed individuals detected indicate that natural gene flow occurs among at least three Ligularia species.Hybrids may become stabilized to form new species or may function as intermediates in evolutionary diversification. | Jiao-Jun YU Chiaki KURODA Xun GONG | 2011 | Journal of Systematics and Evolution2011，49，5： | 5 |
| 18 | Conservation Design for Traditional Agricultural Villages: A Case Study of Shirakawa-go and Gokayama in Japan显示文摘Agricultural villages in Japan are declining and disappearing rapidly.When an agricultural village goes extinct,its tradition and culture die as well.Conserving agricultural villages as cultural heritage sites is one way of preserving them for the future.Agricultural villages can be influenced by the economic and social situation of a country.To safeguard both tangible and intangible cultural traditions,the way that we interpret them is important.Re-designing elements of the landscape may be necessary for conservation,but it may change the natural environment of a village.This paper aims to discuss conservation design in agricultural villages through a case study of the historic villages of Shirakawa-go and Gokayama,which are World Heritage Sites.In Shirakawa mura,there are regulations for the design of landscape elements.Tangible elements are controlled by regulations and guidelines.Gassho-style houses are crucial elements of the design,and each element has a relationship with everyday activities such as agriculture and sericulture.In modern times,relationships with nature have become tenuous,and activity in forest areas has declined.To pass on the traditions and culture of these villages to the next generation,it is important to create new links between each element.An agricultural village cannot continue to be lively without residents.The self-motivation of residents is important for the sustainable development of agricultural villages. | Nobu Kuroda | 2019 | Built Heritage2019，3，2： | 4 |
| 19 | 吸烟状况与急性缺血性脑卒中后功能结局的关系显示文摘吸烟是脑卒中的既定危险因素;然而,脑卒中前吸烟状态是否会影响急性缺血性脑卒中的临床预后尚不确定。该研究旨在阐明吸烟状态与急性缺血性脑卒中后功能预后的关系。研究者获取一项基于日本多中心医院的脑卒中登记资料,调查了2007年7-12月期间住院的10825例急性缺血性脑卒中患者,这些患者在脑卒中发作之前生活能够自理。吸烟状况分为从未吸烟者(不吸烟者),曾经吸烟者和现在吸烟者。临床结局包括3个月时功能转归不良(改良的Rankin量表评分≥2)和功能性依赖(改良的Rankin量表评分2~5)。 | 刘青(译) 刘莉(摘、审校) Matsuo R Ago T Kiyuna F Sato N Nakamura K Kuroda J Wakisaka Y Kitazono T | 2020 | 中华高血压杂志2020，28，2： | 4 |
| 20 | Diagnostic problems in two-dimensional shear wave elastography of the liver显示文摘Two-dimensional shear wave elastography(2D-SWE)is used in the clinical setting for observation of the liver.Unfortunately,a wide spectrum of artifactual images are frequently encountered in 2D-SWE,the precise mechanisms of which remain incompletely understood.This review was designed to present many of the artifactual images seen in 2D-SWE of the liver and to analyze them by computer simulation models that support clinical observations.Our computer simulations yielded the following suggestions:(1)When performing 2D-SWE in patients with chronic hepatic disease,especially liver cirrhosis,it is recommended to measure shear wave values through the least irregular hepatic surface;(2)The most useful 2D-SWE in patients with focal lesion will detect lesions that are poorly visible on B-mode ultrasound and will differentiate true tumors from pseudo-tumors(e.g.,irregular fatty change);and(3)Measurement of shear wave values in the area posterior to a focal lesion must be avoided. | Hiroko Naganuma Hideaki Ishida Atsushi Uno Hiroshi Nagai Hidekatsu Kuroda Masahiro Ogawa | 2020 | World Journal of Radiology2020，12，5： | 3 |
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