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| 1 | Comparative evaluation of microscopy,OptiMAL® and 18S rRNA gene based multiplex PCR for detection of Plasmodium falciparum & Plasmodium vivax from field isolates of Bikaner,India显示文摘Objective:To evaluate microscopy,OptiMAL? and multiplex PCR for the identification of Plasmodium falciparum(P.falciparum) and Plasmodium vivax(P.vivax) from the field isolates of Bikaner,Rajasthan(Northwest India).Methods:In this study,a multiplex PCR(P.falciparum and P.vivax) was further developed with the incorporation of Plasmodium malariae(P.malariae) specific primer and also a positive control.The performance of microscopy,plasmodium lactate dehydrogenase(pLDH) based malaria rapid diagnostic test OptiMAL? and 18S rRNA gene based multiplex PCR for the diagnosis of P.falciparum and P.vivax was compared.Results:The three species multiplex PCR if.falciparum,P.vivax and P.malariae) with an inbuilt positive control was developed and evaluated.In comparison with multiplex PCR,which showed the sensitivity and specificity of 99.36%(95%CI,98.11%-100.00%) and 100.00%(95%CI,100.00%-100.00%),the sensitivity and specificity of microscopy was 90.44%(95%CI,88.849-95.04%) and 99.22%(95% CI,97.71%-100.00%),and OptiMAL? was 93.58%(95%CI,89.75%-97.42%) and 97.69%(95%CI, 95.10%-100.00%).The efficiencies were 99.65%,95.10%and 95.45%for multiplex PCK.microscopy and OptiMAL?.respectively.Conclusions:Our results raise concerns over the overall sensitivities of microscopy and OptiMAL?,when compared to the multiplex PCR and thus stress the need for new molecular interventions in the accurate detection of the malarial parasites.This further highlights the fact that further developments are needed to improve the performance of rapid diagnostic tests at field level. | Deepak Pakalapati Shilpi Garg Sheetal Middha Abhishek Kochar Amit Kumar Subudhi Boopathi Pon Arunachalam Sanjay Kumar Kochar Vishal Saxena Pareek RP Jyoti Acharya Dhanpat Kumar Kochar Ashis Das | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，5： | 3 |
| 2 | Comtmrative evaluation of early versus deferred vitrectomy in Eales disease 显示文摘 | Kumar A Tiwari HK Singh RP | 2000 | Acta Ophthalmol Scand2000，78，： | 1 |
| 3 | Atraumatic convexal subarachnoid hemorrhage:clinical presentation,imaging patterns,and etiologies显示文摘 | Kumar S Goddeau RP Jr Selim MH | 2010 | Neurology2010，74，： | 1 |
| 4 | Atraumatic convex al subarachnoid hemorrhage:clinical presentation,imaging pat- terns , and etiologies显示文摘 | Kumar S Goddeau RP Jr Selim MH | 2010 | Neurology2010，74，11： | 1 |
| 5 | Atraumatic convexal subaraehnoid hemorrhage:clinical presentation, imaging patterns, and etiologies显示文摘 | Kumar S Goddeau RP Jr Selim MH | 2010 | Neurology2010，74，： | 1 |
| 6 | Comparative evaluation of midazolam and ketamine with midazolam alone as oral premedication显示文摘 | Ghai B Grandhe RP Kumar A | 2005 | Paediatr Anaesth2005，15，7： | 1 |
| 7 | Ultrasound-guided brachial plexus blocks 显示文摘 | Kumar RP David M | 2014 | Contin Educ Anaesth Crit Care Pain2014，14，4： | 1 |
| 8 | Ultrasound-induced enhancement of ACh ,ACHE and GABA in fetal brain tissue of mouse显示文摘 | Suneetha N Kumar RP | 1993 | Ultrasound Med Biol1993，19，5： | 1 |
| 9 | Oxidative stress andNrf2in the pathophysiology of diabetic neuropathy:oldperspective with a new angle显示文摘 | Negi G Kumar A Joshi RP | 2011 | Biochem Biophys ResCommun2011，408，1： | 1 |
| 10 | Atraumatic convexal sub- arachnoid hemorrhage: clinical presentation, imaging patterns, and eft- ologies显示文摘 | Kumar S Goddeau RP Jr Selim MH | 2010 | Neurology2010，74，11： | 1 |
| 11 | Selenium ameliorates arsenic induced oxidative stress through modulation of antioxidant enzymes and thiols in rice 显示文摘 | Kumar A Singh RP Singh PK | 2014 | Ecotoxicology2014，23，7： | 1 |
| 12 | Atraumatic convexal subarachnoid hemorrhage显示文摘 | Kumar S Goddeau RP Selim MH | 2010 | Neurology2010，74，11： | 1 |
| 13 | Oxidative stress and Nrf2 in the pathophysiology of diabetic neuropathy : old per- spective with a new angle 显示文摘 | Negi G Kumar A Joshi RP | 2011 | Biocbem Biophys Res Com- mun2011，408，： | 1 |
| 14 | Oxidative stress and diabetic,neu- ropathy : current status of antioxidants 显示文摘 | Negi G Kumar A Joshi RP | 2011 | lnstitue of Integrative Omics and Applied Biotechnology2011，2，6： | 1 |
| 15 | Oxidative stress and diabetic neuropathy:current status of antioxidants显示文摘 | Negi G Kumar A Joshi RP | 2011 | Institute of Integrative Omics and Applied Biotechnology2011，2，6： | 1 |
| 16 | Bankruptcy Prediction in Banks and Firms via Statistical and Intelligent Tech- niques-a Review 显示文摘 | ]Kumar RP Ravi V | 2007 | European Journal of Operational Research2007，180，： | 1 |
| 17 | Comparative evaluation of midazolam and ketamine with midazolam alone as oral premedication显示文摘 | Ghai B Grandhe RP Kumar A | 2005 | Paediatr Anaesth2005，15，7： | 1 |
| 18 | Oxidative stress and Nrf2 in the pathophysiology of diabetic neuropathy: old perspective with a new angle显示文摘 | Negi G Kumar A Joshi RP | 2011 | Biochem Biophys Res Commun2011，408，1： | 1 |
| 19 | Isolated malacoplakia of the bladder: a rare case report and review of literature显示文摘 | Mukha RP Kumar S Ramani MK | 2010 | Int Urol Nephrol2010，42，2： | 1 |
| 20 | Intraaortic balloon pump boon for off-pump coronary artery bypass grafting 显示文摘 | Saha KK Kaushal RP Kumar A | 2014 | Asian Car- diovasc Thorac Ann2014，，： | 1 |
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