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| 1 | Integration of hepatitis B virus DNA into chromosomal DNA during acute hepatitis B显示文摘AIM: To examine the serum from black African patients with acute hepatitis B to ascertain if integrants of viral DNA can be detected in fragments of cellular DNA leaking from damaged hepatocytes into the circulation.METHODS: DNA was extracted from the sera of five patients with uncomplicated acute hepatitis B and one with fulminant disease. Two subgenomic PCRs designed to amplify the complete genome of HBV were used and the resulting amplicons were cloned and sequenced.RESULTS: HBV and chromosomal DNA were amplified from the sera of all the patients. In one patient with uncomplicated disease, HBV DNA was integrated into host chromosome 7 q11.23 in the WBSCR1 gene. The viral DNA comprised 200 nucleotides covering the S and X genes in opposite orientation, with a 1 169 nudeotide deletion. The right virus/host junction was situated at nucleotide 1774 in the cohesive overlap region of the viral genome, at a preferred topoisomerase I cleavage motif. The chromosomal DNA was not rearranged.The patient made a full recovery and seroconverted to anti-HBs- and anti-HBe-positivity. Neither HBV nor chromosomal DNA could be amplified from his serum at that time.CONCLUSION: Integration of viral DNA into chromosomal DNA may occur rarely during acute hepatitis B and, with clonal propagation of the integrant, might play a role in hepatocarcinogenesis. | Gerald C Kimbi Anna Kramvis Michael C Kew | 2005 | World Journal of Gastroenterology2005，11，41： | 11 |
| 2 | HBV/HIV共感染与单纯感染HBV 1762T/1764A突变及前S基因缺失的比较研究显示文摘目的比较分析HBV/HIV共感染者与HBV单纯感染者肝癌相关HBV 1762T/1764A突变及前S基因缺失突变率。方法根据病例对照研究原理,收集未接受HBV及HIV抗病毒治疗的HBV/HIV共感染者和HBV单纯感染者血清标本,用分子生物学技术对血清HBV核心基因启动子(BCP)和前S基因进行分析。结果病例-对照共61对,平均年龄为(44.9±2.1)岁,共发现3个HBV基因型:基因型B、C和I。共感染者和单纯感染者的主要基因型均为B基因型,分别占63.4%(39/61)和50.8%(31/61)。共感染者肝癌相关HBV基因突变总率(52.5%)显著高于HBV单纯感染者(23.0%),差异有统计学意义(χ~2=11.307,P<0.05)。共感染者BCP的1762T/1764A双突变率(44.3%)显著高于HBV单纯感染者(χ~2=7.290,P<0.05)。同样,共感染者前S基因缺失率也显著高于HBV单纯感染者(χ~2=8.270,P<0.05)。随机从两组中各抽20例进行对比分析,结果显示共感染与高病毒载量有关,但是高病毒载量与肝癌相关HBV基因突变无关。肝癌相关HBV基因突变率不随CD_4^+细胞计数变化而变化。多因素分析结果表明,合并HIV感染与肝癌相关HBV基因突变(包括BCP双突变与前S缺失突变)有关。结论 HBV/HIV共感染可增加肝癌相关HBV基因突变率。 | 李开文 梁淑家 陈钦艳 王学燕 杨庆利 何翔 胡丽萍 贾蕙华 Anna Kramvis 方钟燎 | 2017 | 应用预防医学2017，23，2： | 4 |
| 3 | Follow up of infection of chacma baboons with inoculum containing a and non-a genotypes of hepatitis B virus显示文摘AIM: To determine whether one genotype (A or non-A genotypes of HBV) predominated over the other during the course of HBV infection.METHODS: Four baboons were inoculated with HBV. DNA was extracted from serum obtained at monthly intervals postinoculation for 52 weeks and HBV DNA was amplified using primers specific for the core region containing an insert characteristic of genotype A (nt 2 354-2 359, numbering from the EcoRI site). The amplicons were cloned into PCRScriptTM and a minimum of 15 clones per time point were sequenced in both directions.RESULTS: Both genotypes persisted for the entire followup period of 52 weeks. Genotype non-A predominated in two baboons and genotype A in one baboon. Neither genotype predominated in the fourth baboon, as shown at a 5 % level of testing.CONCLUSION: No conclusions concerning the dominance of either genotype or the natural progression or replication rates of HBV could be drawn because the pattern of the genotypes found may have been caused by sampling fluctuations at the time of DNA extraction and cloning as a result of the very low viral loads in the baboon sera. | Marina Baptista Anna Kramvis Saffie Jammeh Jocelyn Naicker Jacqueline S. Galpin Michael C. Kew | 2003 | World Journal of Gastroenterology2003，9，4： | 4 |
| 4 | Hepatitis B virus DNA in serum of healthy black African adults positive for hepatitis B surface antibody alone: possible association with recombination between genotype A and D显示文摘 | Owiredu WK Kramvis A Kew MC | 2001 | J Med Virol2001，64，： | 1 |
| 5 | Hepatitis B virus precore mutants in serum and liver of Southern African Blacks with hepatocellular carcinoma显示文摘 | Anna Kramvis Michael C Kew Stanley Bukofzer | 1998 | Journal of Hepatology1998，，1： | 1 |
| 6 | Molecular Characterization of Duck Hepatitis B Virus Isolates from South African Ducks 显示文摘 | MANGISA N P SMUTS H E KRAMVIS A | 2004 | Virus Genes2004，28，： | 1 |
| 7 | Molecular analysis of hepatitis B virus genomes isolated from black African patients with fulminant hepatitis B显示文摘 | Kramvis A Kew MC | 2001 | J Med Virol2001，65，3： | 1 |
| 8 | Nucleic acid sequence analysis of the precore region of hepatitis B virus from sera of southern African black adult carriers of the virus显示文摘 | A Kramvis S Bukofzer MC Kew E Song | 1997 | Hepatology1997，，1： | 1 |
| 9 | Hepatitis B virus genotypes显示文摘 | Kramvis A Kew M Francois G | 2005 | Vaccine2005，23，： | 1 |
| 10 | Distinctive sequence characteristics of subgenotype A isolates of hepatitis B virus from South Africa显示文摘 | Kimbri GC Kramvis A Kew MC | 2004 | J GEN Virol2004，85，5： | 1 |
| 11 | Hepatitis B virus genolypes 显示文摘 | Anna Kramvis Michael Kew Guido Francois | 2005 | Vaccine2005，23，24： | 1 |
| 12 | Hepatitis B virus DNA in serum of healthy black African adults positive for hepatitis B surface antibody alone:possible association with recombination between genotypes A and D显示文摘 | Owiredu WK Kramvis A Kew MC | | 0，，： | 1 |
| 13 | Comparison of hepatitis B virus DNA extractions from serum by the QIAamp blood kit,GeneReleaser,and the phenol-chloroform method显示文摘 | Kramvis A Bukofzer S Kew MC | 1996 | J Clin Microbiol1996，34，11： | 1 |
| 14 | High prevalence of 1762(T) 1764(A) mutations in the basic core promoter of hepatitis B virus isolated from black Africans with hepatocellular carcinoma compared with asymptomatic carriers显示文摘 | Baptista M Kramvis A Kew MC | 1999 | Hepatology1999，，： | 1 |
| 15 | Molecular characterization of duck hepatitis B virus isolates from South African ducks显示文摘 | Mangisa N P Smuts H E Kramvis A | 2004 | Virus Genes2004，28，2： | 1 |
| 16 | Treatment of hepatitis B virus-associated nephropathy in black children显示文摘 | Bhimma R coovadia HM Kramvis A | 2002 | Pedliatr Nephrol2002，17，： | 1 |
| 17 | Hepatitis B virus precore mutants in serum and liver of southern african blacks with hepatocellular carcinoma显示文摘 | Kramvis A Kew M C Bukofzer S | 1998 | J Hepatol1998，28，： | 1 |
| 18 | Treatment of hepatitis B virus- associated nephropathy in black children 显示文摘 | Bhimma R Coovadia H M Kramvis A | 2002 | Pediatr NephroI2002，17，： | 1 |
| 19 | The core promoter of hepatitis B virus显示文摘 | Kramvis A and Kew MC | 1999 | J Viral Hepat1999，6，： | 1 |
| 20 | High prevalence of 1762T 1764A mutation in the basic core promoter of hepatitis B virus isolated from black Africans with hepatocellular carcinoma compared with asymptomatic carriers显示文摘 | Marina B Kramvis A and Michael CK | 1999 | Hepatology1999，29，： | 1 |
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