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| 1 | Acute hepatitis C in a chronically HIV-infected patient:Evolution of different viral genomic regions显示文摘AIM: To analyze the molecular evolution of different viral genomic regions of HCV in an acute HCV infected patient chronically infected with HIV through a 42-month follow-up.METHODS: Serum samples of a chronically HIV infected patient that seroconverted to anti HCV antibodies were sequenced, from the event of superinfection through a period of 17 months and in a late sample (42nd month). Hypervariable genomic regions of HIV (V3 loop of the gp120) and HCV (HVR-1 on the E2 glycoprotein gene) were studied. In order to analyze genomic regions involved in different biological functions and with the cellular immune response, HCV core and NS5A were also chosen to be sequenced. Amplification of the different regions was done by RT-PCR and directly sequenced. Confirmation of sequences was done on reamplified material. Nucleotide sequences of the different time points were aligned with CLUSTAL W 1.5, and the corresponding amino acid ones were deduced.RESULTS: Hypervariable genomic regions of both viruses (HVR1 and gp120 V3 loop) presented several nonsynonymous changes but, while in the gp120 V3 loop mutations were detected in the sample obtained right after HCV superinfection and maintained throughout, they occurred following a sequential and cumulative pattern in the HVR1. In the NS5A region of HCV, two amino acid changes were detected during the follow-up period, whereas the core region presented several amino acid replacements, once the HCV chronic infection had been established.CONCLUSION: During the HIV-HCV superinfection, each genomic region analyzed shows a different evolutionary pattem.Most of the nucleotide substitutions observed are nonsynonymous and clustered in previously described epitopes,thus suggesting an immune-driven evolutionary process. | Diego Flichman Veronica Kott Silvia Sookoian Rodolfo Campos | 2003 | World Journal of Gastroenterology2003，9，7： | 2 |
| 2 | A new allele specific polymerase chain reaction method (AS-PCR) for detection of the goat CSN1S1 01 allele 显示文摘 | Sztank'oov'a Z Kott T Czemekov'a V | 2006 | Small Ruminant Res2006，66，1： | 1 |
| 3 | Increase in left ventricular torsion-to-shortening ratio in children with valvular aortic stenosis显示文摘 | Delhaas T Kotte J van der Toorn A | 2004 | MagnResonMed2004，51，13： | 1 |
| 4 | Developmental localization of GAP-43 and olfactory marker protein in rat olfactory bulb transplants 显示文摘 | Kott JN Westrum LE | 1996 | Int J Dev Neurosci1996，14，： | 1 |
| 5 | Characterization of clinical isolates of Escherichia coli showing high levels of fluoroquinolone resistance显示文摘 | Lehn N Stower-Hoffmann J Kott T | 1996 | Journal of Clinical Microbiology1996，34，3： | 1 |
| 6 | Comparative effect of TGF-betal, -beta2,and-beta3 on extracellular matrix production, proliferation, migration, and collagen contraction of human Tenon's capsule fibroblasts in pseudoex foliation and primary open angle glaucoma显示文摘 | Kott|er RU Junemann AG Aigner Y | 2005 | Exp Eye Res2005，80，2： | 1 |
| 7 | Hypertensive putaminal hemorrhage presenting with hemichorea显示文摘 | Jones HR Jr Baker RA Kott HS | | 0，，2： | 1 |
| 8 | Development of a humanized disulfide-stabilized anti-p185HER2 Fv-beta-lactamase fusion protein for activation of a cephalosporin dexorubicin prodrug显示文摘 | Rodriguea ML Presta LG Kotts CE | 1995 | Cancer Res1995，55，1： | 1 |
| 9 | Effect of secondhand cigarette smoke,RSV bronchiolitis and parental asthma on urinary cysteinyl LTE4显示文摘 | Kott KS Salt BH McDonald RJ | 2008 | Pediatr Pulmonol2008，43，76： | 1 |
| 10 | Hypertensive putaminal hemorrhage presenting with hemichorea显示文摘 | Jones HR Baker RA Kott S | | 0，，1： | 1 |
| 11 | Strintium modification gives critical melt control 显示文摘 | Kotte B | 1985 | Modern Casting1985，，5： | 1 |
| 12 | Autotoxicity in isolated microspore culture of Brassica napus显示文摘 | Kott LS Polsoni L | 1998 | Can J Bot1998，66，： | 1 |
| 13 | Effect of Halogens on Algae显示文摘 | Betzer N Kott Y | 1969 | Wat Res1969，，3： | 1 |
| 14 | Modelling tissue heating with ferromagnetic seeds 显示文摘 | Kotte AN van Wieringen N Lagendijk JJ | 1998 | Phys Med Biol1998，43，1： | 1 |
| 15 | Hypertensive putaminal hemor-rhage presenting with hemichorea 显示文摘 | Jones HR Baker RA Kott Hs | 1985 | Stroke1985，16，： | 1 |
| 16 | Diet quality of older Americans in 1994-1996 and 2001-2002 as measured by the Healthy Eating Index-2005 显示文摘 | Juan W Y Guenther P M Kott P S | 2008 | Nutrition insights2008，，： | 1 |
| 17 | Rate and duration of grain filling in durum wheat cultivars显示文摘 | Gebeyehou G Kott D R Baker R J | 1982 | Crop Science1982，22，： | 1 |
| 18 | Rate and duration of grain filling in durum wheat cultivars显示文摘 | Gebeyehou G Kott D R Baker R J | 1982 | Crop Sci1982，22，： | 1 |
| 19 | Effects of Cross-Linkage in Wrinkle-Resistant Cotton Fabrics 显示文摘 | Frick J G Kottes A B A Reid J D | 1960 | Textile Research Journal1960，30，7： | 1 |
| 20 | Characterization of clinical isolates of Escherichia coli showing high levels of fluoroquinolone resistance显示文摘 | Lehn N Stower-Hoffmann J Kott T | 1996 | J Clin Microbiol1996，34，3： | 1 |
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