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| 1 | Potential role of human papilloma virus in the pathogenesis of gastric cancer显示文摘AIM:To demonstrate the presence and biological activity of human papilloma virus(HPV)in gastric cancer(GAC)tissues.METHODS:The study involved 84 surgically treated patients with gastric adenocarcinoma,regardless of the clinical stage of the disease.The presence of HPV DNA of high oncogenic risk types in formalin-fixed,paraffinembedded tumor samples was determined using quantitative polymerase chain reaction analysis.A stringentprotocol of prevention of cross-and environmental contamination was applied during DNA isolation,and amplification,as well as confirmation of the biological activity of the virus in tumor cells,was implemented.The study utilized the Real-time High Risk HPV test,which detects the DNA of 14 HPV subtypes that are considered to have high oncogenic potential.The overexpression of the p16INK4a protein assessed immunohistochemically was considered confirmation of the HPV infection.RESULTS:Among the 89 patients initially included in the study group,diagnostic results were obtained for84 individuals.In five cases,either the histopathological material was too scant to isolate the necessary amount of DNA,or the isolated DNA was significantly degraded,resulting in the failure of internal control amplification within the predefined number of 35 cycles.Those patients were excluded from further analysis.The amplification of HPV DNA was demonstrated in none of the84 tissue samples;thus,all cases were considered to have a negative DNA status of highly oncogenic HPV subtypes.Immunohistochemical staining provided diagnostic results for all of the examined tissue samples,and excluded the accumulation of the p16INK4a protein in tumor cells,thus confirming the lack of active HPV infection in all of the individuals.CONCLUSION:The study does not confirm the presence or biological activity of HPV in tumor tissues.Thus,the relationship between GAC and HPV infection,in the Central European population seems doubtful. | Miroslaw Snietura Dariusz Waniczek Wojciech Piglowski Agnieszka Kopec Ewa Nowakowska-Zajdel Zbigniew Lorenc Malgorzata Muc-Wierzgon | 2014 | World Journal of Gastroenterology2014，20，21： | 2 |
| 2 | Measuring functional outcomes in persons with back pain:a review of back-specific questionnaires显示文摘 | Kopec JA | 2000 | Spine2000，25，24： | 1 |
| 3 | Evapotranspiration of tall fescue turf显示文摘 | Kopec D M Shearman R C Riordan T P | 1988 | HortScience1988，47，： | 1 |
| 4 | Electrocardiogram for the diagnosis of right ventricular hypertrophy and dilation in idiopathic pulmonary arterial hypertension显示文摘 | Kopec G Tyrka A Miszalski-Jamka T | | 0，，07： | 1 |
| 5 | Evapotranspiration of tall fescue turf显示文摘 | Kopec D M Shearman R C Riordan T P | 1988 | HortScience1988，23，2： | 1 |
| 6 | Design and tolerance analysis of alldielectric non-polarizing beamsplitter coatings 显示文摘 | KOPEC G J | 2005 | SPIE2005，5954，： | 1 |
| 7 | Growth factor signaling and memory formation: temporal and spatial integration of a molecular network显示文摘 | Kopec AM Carew TJ | 2013 | Learn Mem2013，20，10： | 1 |
| 8 | Further observations of the urban heat island in a small city 显示文摘 | Richard J Kopec | 1970 | Bull Amer Meteor Soc1970，51，7： | 1 |
| 9 | Herpesviruses as possible co-factors in HPV-16-related oncogenesis显示文摘 | Szostek S Zawilinska B Kopec J | 2009 | ActaBiochimPol2009，56，： | 1 |
| 10 | Quality of llfe in operable colon cancer patients receiving oral compared with intravenous chemotherapy:results from National Surgical Adjuvant Breast and Bowel Project Trial C-06显示文摘 | Kopec JA Yothers G Ganz PA | 2007 | J Clin Oncol2007，25，4： | 1 |
| 11 | Roles of stargazin and phosphorylation in the control of AMPA receptor subcellular distribution显示文摘 | Kessels HW Kopec CD Klein ME | 2009 | Nat Neurosci2009，12，7： | 1 |
| 12 | Randomized, doubleblind, placebo-controlled glucosamine discontinuation trial in knee osteoarthritis 显示文摘 | Cibere J Kopec JA Thome A | 2004 | Arthritis Rheum2004，51，5： | 1 |
| 13 | ROC are a discrimination (ROCAD) curve:a new method of evaluating the discriminating ability of ordinal scales显示文摘 | Kopec JA Sayre EC | 2008 | J Clin Epidemiol2008，61，10： | 1 |
| 14 | Randomized, double-blind, placebo-controlled glucosamine discontinuation trial in knee os- teoarthritis显示文摘 | Cibere J Kopec JA Thorne A | 2004 | Arthritis Rheum2004，51，5： | 1 |
| 15 | GluR1 links structural and functional plasticity at excitatory synapses显示文摘 | Kopec CD Real E Kessels HW | 2007 | J Neurosci2007，27，13： | 1 |
| 16 | Lymphotropic herpesvirus DNA detection in patients with active CMV infection-a possible role in the course of CMV infection after hematopoietic stem cell transplantation显示文摘 | Zawilinska B Kopec J Szostek S | | 0，，08： | 1 |
| 17 | Herpesviruses as possible co- factors in HPV- 16- related oncogenesis显示文摘 | Szostek S Zawilinska B Kopec J | 2009 | Acta Biochim Pol2009，56，2： | 1 |
| 18 | Relative salinity tolerance of 21 turf-type desert saltgrasses compared to bermuda grass显示文摘 | Root and verdure Marcum K B Pessarakli M Kopec D M | 2005 | Hortscience2005，40，3： | 1 |
| 19 | Herpesviruses as possible co- factors in HPV-16-related oncogenesis显示文摘 | Szostek S Zawilinska B Kopec J | 2009 | Acta Biochim Pol2009，56，2： | 1 |
| 20 | The Ex- perimental Exploration of Embedding Phase Change Ma- terials With Graphite Nanofibers for the Thermal Man- agement of Electronics 显示文摘 | Weinstein R D Kopec T C Fleischer A S | 2008 | Journal of Heat Transfer2008，130，04： | 1 |
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