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| 1 | Direct laser writing of volumetric gradient index lenses and waveguides显示文摘Direct laser writing(DLW)has been shown to render 3D polymeric optical components,including lenses,beam expanders,and mirrors,with submicrometer precision.However,these printed structures are limited to the refractive index and dispersive properties of the photopolymer.Here,we present the subsurface controllable refractive index via beam exposure(SCRIBE)method,a lithographic approach that enables the tuning of the refractive index over a range of greater than 0.3 by performing DLW inside photoresist-filled nanoporous silicon and silica scaffolds.Adjusting the laser exposure during printing enables 3D submicron control of the polymer infilling and thus the refractive index and chromatic dispersion.Combining SCRIBE’s unprecedented index range and 3D writing accuracy has realized the world’s smallest(15μm diameter)spherical Luneburg lens operating at visible wavelengths.SCRIBE’s ability to tune the chromatic dispersion alongside the refractive index was leveraged to render achromatic doublets in a single printing step,eliminating the need for multiple photoresins and writing sequences.SCRIBE also has the potential to form multicomponent optics by cascading optical elements within a scaffold.As a demonstration,stacked focusing structures that generate photonic nanojets were fabricated inside porous silicon.Finally,an all-pass ring resonator was coupled to a subsurface 3D waveguide.The measured quality factor of 4600 at 1550 nm suggests the possibility of compact photonic systems with optical interconnects that traverse multiple planes.SCRIBE is uniquely suited for constructing such photonic integrated circuits due to its ability to integrate multiple optical components,including lenses and waveguides,without additional printed supports. | Christian R.Ocier Corey A.Richards Daniel A.Bacon-Brown Qing Ding Raman Kumar Tanner J.Garcia Jorik van de Groep Jung-Hwan Song Austin J.Cyphersmith Andrew Rhode Andrea N.Perry Alexander J.Littlefield Jinlong Zhu Dajie Xie Haibo Gao Jonah F.Messinger Mark L.Brongersma Kimani C.Toussaint Jr Lynford L.Goddard Paul V.Braun | 2020 | Light(Science & Applications)2020，9，1： | 6 |
| 2 | Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)显示文摘In higher plants, vacuolar invertases play essential roles in sugar metabolism, organ development, and sink strength. In sorghum(Sorghum bicolor), two vacuolar invertase genes,Sb VIN1(Sobic. 004 G004800) and Sb VIN2(Sobic. 006 G160700) have been reported, but their enzymatic properties and functional differences are largely unknown. We combined molecular, biochemical and genomic approaches to investigate their roles in sorghum stem and grain traits. Sb VIN1 and Sb VIN2 showed different expression levels in internodes,leaves, and panicles, indicating that their importance in each organ was different. In an in vitro sucrose hydrolysis assay, proteins of both genes heterologously expressed in Pichia pastoris displayed similar enzyme properties including the same optimum reaction p H(5)and similar Kmfor sucroe(49 mmol L-1 and 45 mmol L-1 for Sb VIN1 and Sb VIN2,respectively). The optimum reaction temperatures of Sb VIN1 and Sb VIN2 were 45 °C and65 °C, respectively. Sb VIN2 showed higher tolerance to high temperature than Sb VIN1. We characterized the sequence variation of these two vacuolar invertase genes in a panel of 216 diverse inbred lines of sweet and grain sorghum and performed gene-based association analysis. Sb VIN1 showed significant associations with stem traits including stem length,stem diameter, internode number, stem fresh weight, and Brix, as well as grain traits including hundred-grain weight and grain width. Significantly associated variation sites were mainly in 5′ upstream and intron regions. Sb VIN2 only associated with grain width and stem water-soluble carbohydrates(WSCs) content. We conclude that the vacuolar invertase genes Sb VIN1 and Sb VIN2 are differently associated with stem and grain traits in sorghum. | Yunhua Chi Kimani Wilson Zhiquan Liu Xiaoyuan Wu Li Shang Limin Zhang Haichun Jing Huaiqing Hao | 2020 | The Crop Journal2020，8，2： | 2 |
| 3 | Influ- ence of HLA supertypes on susceptibility and resistance to human immanodeficiency virus type 1 infection显示文摘 | MACDONALD K S FOWKE K R KIMANI J | 2000 | J infect Dis2000，181，5： | 1 |
| 4 | Probing the antioxidant action of selenium and sulfur using Cu (I) -chalcogenone tris(pyrazolyl) meth- ane and -borate complexes显示文摘 | Kimani M M Brumaghim J L VanDerveer D | 2010 | Inorg Chem2010，49，20： | 1 |
| 5 | Influence of HLA supertypes on susceptibility and resistance to human immunodeficiency virus type 1 infection显示文摘 | MacDonald KS Fowke KR Kimani J | 2000 | J Infect Dis2000，181，： | 1 |
| 6 | Resistance to HIV-1 infection among persistently seronegative prostitutes in Nairobi,Kenya 显示文摘 | Fowke KR Nagelkerke NJ Kimani J | 1996 | lancet1996，348，： | 1 |
| 7 | Tumor M2-pyiuvate ki- nase: a gastrointestinal cancer marker 显示文摘 | Kumar Y Tapuria N Kimani N | 2007 | Eur J Gastroenter- ol Hepatol2007，19，3： | 1 |
| 8 | Translating the immunogenicity of prime-boost immunization with ChAd63 and MVA ME-TRAP from malaria naive to malaria- endemic populations 显示文摘 | Kimani D Jagne YJ Cox M | 2014 | Mol Ther2014，22，11： | 1 |
| 9 | Retinoblastoma 显示文摘 | Dimaras H Kimani K Dimba EA | 2012 | Lancet2012，379，9824： | 1 |
| 10 | 一种碱性γ-谷氨酰转肽酶在Bacillus subtilis 168中的分泌表达与酶学性质分析显示文摘将γ-谷氨酰转肽酶(GGT)在Bacillus subtilis 168同源过量表达,发现该酶能够自动分泌到细胞外,且重组菌酶活比原始菌提高了3 568倍。通过SDS-PAGE分析发酵液上清液发现,该γ-谷氨酰转肽酶是由一个相对分子质量为42 000的大亚基和一个22 000的小亚基组成。通过酶学性质分析发现,该γ-谷氨酰转肽酶是一个耐碱性的酶,最适反应pH为10;同时该酶具有良好的耐热性,最适反应温度达到50℃;另外,加入5.0 mmol/L的Mg^(2+)、Ca^(2+)、K^+、La^(3+)、Li^+和NH_4^+对GGT转肽活力具有明显的促进作用,其中添加的NH_4^+对GGT活力促进作用最为显著,GGT活力提高45%以上,而Cu^(2+)和Zn^(2+)则对其具有抑制作用。 | Bernard Gitura Kimani 杨套伟 饶志明 张显 徐美娟 和斐 | 2017 | 食品与生物技术学报2017，36，2： | 1 |
| 11 | Retinoblastoma 显示文摘 | Dimaras H Kimani K Dimba EA | 2012 | Lancet2012，379，： | 1 |
| 12 | Retinoblastoma显示文摘 | Dimaras H Kimani K Dimba EA | | 0，，9824： | 1 |
| 13 | Graduates perception of university pmgranunes and their relevance to employment:University of Naimbi (1991-1998) 显示文摘 | Gerald Ngugi Kimani | 2005 | Africa Development2005，30，12： | 1 |
| 14 | IL-7- Ralpha expression on CD4+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation 显示文摘 | Koesters SA Alimonti JB Wachihi C Matu L Anzala O Kimani J Embree JE Plummer FA Fowke KR | 2006 | Eur J Immunol2006，36，2： | 1 |
| 15 | Retinoblastoma显示文摘 | Dimaras H Kimani K Dimba EA | 2012 | Lan cet2012，379，9824： | 1 |
| 16 | PDGF - Ralpha gene ex- pression predicts proliferation,but PDGF - A suppresses transdifferentiation of neonatal mouse lung myofibroblasts 显示文摘 | Kimani PW Holmes A J Grossmann RE | 2009 | Respir Res2009，10，119： | 1 |
| 17 | Biosystematics of the Cotesia flavipes complex (Hymenoptera: Braconidae):interspec- ific hybridization, sex pheromone and mating behaviour studies 显示文摘 | Kimani S W Overholt W A | 1995 | Bull Ent Res1995，85，： | 1 |
| 18 | Assessment of energy performance and air pollutant emis- sions in a diesel engine generator fueled with water-contai- ning ethanol-biodiesel-diesel blend of fuels 显示文摘 | Wen-Jhy Lee Yi-Cheng Liu Francis Kimani Mwangi | 2011 | Energy2011，36，9： | 1 |
| 19 | Influence of HLA Su- pertypes on Susceptibility and Resistance to Human Immunodefi- ciency Virus Typel Infection 显示文摘 | MacDonald KS Fowke KR Kimani J | 2000 | J Infect Dis2000，181，5： | 1 |
| 20 | Is pre-hypertensioo a risk factor for cardiovascular diseases? 显示文摘 | Qureshi AI SuriMFK Kimani JF | 2005 | Stroke2005，36，9： | 1 |
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