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| 1 | A novel animal model for in vivo study of liver cancer metastasis显示文摘AIM:To establish an animal model with human hepatocyte-repopulated liver for the study of liver cancer metastasis.METHODS:Cell transplantation into mouse livers was conducted using alpha-fetoprotein(AFP)-producing hu-man gastric cancer cells(h-GCCs) and h-hepatocytes as donor cells in a transgenic mouse line expressing urokinase-type plasminogen activator(uPA) driven by the albumin enhancer/promoter crossed with a severe combined immunodeficient(SCID) mouse line(uPA/SCID mice).Host mice were divided into two groups(A and B).Group A mice were transplanted with h-GCCs alone,and group B mice were transplanted with h-GCCs and h-hepatocytes together.The replacement index(RI),which is the ratio of transplanted h-GCCs and h-hepatocytes that occupy the examined area of a histological section,was estimated by measuring h-AFP and h-albumin concentrations in sera,respectively,as well as by immunohistochemical analyses of h-AFP and human cytokeratin 18 in histological sections.RESULTS:The h-GCCs successfully engrafted,repopulated,and colonized the livers of mice in group A(RI = 22.0% ± 2.6%).These mice had moderately differentiated adenocarcinomatous lesions with disrupted glandular structures,which is a characteristics feature of gastric cancers.The serum h-AFP level reached 211.0 ± 142.2 g/mL(range,7.1-324.2 g/mL).In group B mice,the h-GCCs and h-hepatocytes independently engrafted,repopulated the host liver,and developed colonies(RI = 12.0% ± 6.8% and 66.0% ± 12.3%,respectively).h-GCC colonies also showed typical adenocarcinomatous glandular structures around the h-hepatocyte-colonies.These mice survived for the full 56 day-study and did not exhibit any metastasis of h-GCCs in the extrahepatic regions during the observational period.The mice with an h-hepatocyte-repopulated liver possessed metastasized h-GCCs and therefore could be a useful humanized liver animal model for studying liver cancer metastasis in vivo.CONCLUSION:A novel animal model of human liver cancer metastasis was established using the uPA/SCID mouse line.This model could be useful for in vivo testing of anti-cancer drugs and for studying the mechanisms of human liver cancer metastasis. | Shinsuke Fujiwara Hikaru Fujioka Chise Tateno Ken Taniguchi Masahiro Ito Hiroshi Ohishi Rie Utoh Hiromi Ishibashi Takashi Kanematsu Katsutoshi Yoshizato | 2012 | World Journal of Gastroenterology2012，18，29： | 6 |
| 2 | Optimal Restoration Scheduling of Damaged Networks Under Uncertain Environment by Using Improved Genetic Algorithm显示文摘The purpose of this research is to propose an early restoration for lifeline systems after earthquake disasters. The previous researches show that the optimization of the restoration schedule by using genetic algorithm (GA) is powerful. However, those are not considering the uncertain environment after earthquake disasters. The circumstances of the damage at devastated areas are very changeable due to the aftershock, fire disaster and bad weather. In addition, the restoring works may delay by unexpected accidents. Therefore, it is necessary to obtain the restoration schedule which has robustness, because the actual restoring works could not progress smoothly under the uncertain environment. GA considering uncertainty (GACU) can treat various uncertainties involved, but it is difficult to obtain the robust schedule. In this study, an attempt is made to develop a decision support system of the optimal restoration scheduling by using the improved GACU. | Hitoshi FURUTA Ken ISHIBASHI Koichiro NAKATSU Shun HOTTA | 2008 | Tsinghua Science and Technology2008，13，S1： | 3 |
| 3 | 在有 vasospastic 咽峡炎的病人的冠的 microvascular 功能的评估显示文摘 AIM: To investigate endothelium-dependent and independent coronary microvascular functions in patients with vasospastic angina (VSA). METHODS: Thirty-six patients with VSA (30 men and 6 women; mean age, 58 years) were enrolled in this study. VSA was defined as ≥ 90% narrowing of the epicardial coronary arteries on angiography performed during a spasm provocation test, presence of chest pain, and/or ST-segment deviation on an electrocardiogram (ECG). Patients (n = 36) with negative spasm provocation test results and those matched for age and sex were enrolled as a control group (nonVSA group). Low-dose acetylcholine (ACh; 3 μg/min) was infused into the left coronary ostium for 2 min during the spasm provocation test. Following the spasm provocation test, nitroglycerin (0.2 mg) was administered intracoronally. Coronary blood flow (was calculated from quantitativeangiography and Doppler flow velocity measurements, and the coronary flow reserve was calculated as the ratio of coronary flow velocity after injection of adenosine triphosphate (20 μg) to the baseline value. Changes in the coronary artery diameter in response to ACh and nitroglycerin infusion were expressed as percentage changes from baseline measurements. RESULTS: Body mass index was significantly lower in the VSA group than in the nonVSA group. The fre- quency of conventional coronary risk factors and the rate of statin use were similar between the 2 groups. The left ventricular ejection fraction as evaluated by echocardiography was similar between the 2 groups. The duration of angina was 9 ± 2 mo. The results of blood chemistry analysis were similar between the 2 groups. Low-dose ACh did not cause coronary spasms. The change in coronary artery diameter in response to ACh was lower in the VSA group (-1.4% ± 9.3%) than in the nonVSA group (3.1% ± 6.5%, P < 0.05), whereas nitroglycerin-induced coronary artery dilatation and coronary blood flow increase in response to ACh or coronary flow reserve did not differ significantly between the 2 groups. CONCLUSION: These findings suggest that microvascular coronary function may be preserved despite endothelial dysfunction of the epicardial coronary arteries in patients with VSA. | Hiroki Teragawa Naoya Mitsuba Ken Ishibashi Kenji Nishioka Satoshi Kurisu Yasuki Kihara | 2013 | World Journal of Cardiology2013，5，1： | 2 |
| 4 | Blocking of monocyte chemoattractant protein-1 (MCP-1) activity attenuates the severity of acute pancreatitis in rats显示文摘 | Toshiaki Ishibashi Haifeng Zhao Ken Kawabe Takamasa Oono Kensuke Egashira Koichi Suzuki Hajime Nawata Ryoichi Takayanagi Tetsuhide Ito | 2008 | Journal of Gastroenterology2008，，1： | 1 |
| 5 | RSV replication is attenuated by counteracting expression of the suppressor of cytokine signaling (SOCS) molecules显示文摘 | Koichi Hashimoto Kei Ishibashi Ken Ishioka Dongchi Zhao Masatoki Sato Shinichiro Ohara Yusaku Abe Yukihiko Kawasaki Yuka Sato Shin-ichi Yokota Nobuhiro Fujii Ray Stokes Peebles Mitsuaki Hosoya Tatsuo Suzutani | 2009 | Virology2009，，2： | 1 |
| 6 | Preliminary results of a Japanese nationwide survey of neuroendocrine gastrointestinal tumors显示文摘 | Tetsuhide Ito Masao Tanaka Hironobu Sasano Yoshiyuki R. Osamura Iwao Sasaki Wataru Kimura Koji Takano Takao Obara Miyuki Ishibashi Kazuwa Nakao Ryuichiro Doi Akira Shimatsu Toshirou Nishida Izumi Komoto Yukio Hirata Masayuki Imamura Ken Kawabe Kazuhiko Na | 2007 | Journal of Gastroenterology2007，，6： | 1 |
| 7 | Blocking of monocyte chemoattractant protein-1 (MCP-1) activity attenuates the severity of acute pancreatitis in rats显示文摘 | Toshiaki Ishibashi Haifeng Zhao Ken Kawabe Takamasa Oono Kensuke Egashira Koichi Suzuki Hajime Nawata Ryoichi Takayanagi Tetsuhide Ito | 2008 | Journal of Gastroenterology2008，，1： | 1 |
| 8 | Gene transfer of stromal cell - derive factor - 1 enhances ischemic vasculogenesis and angiogenesis via vascular endothelial growth factor/endothelial nitric oxide synthase - related pathway: next - generation chemokine therapy for therapeutic Nnovascularization 显示文摘 | Ken - ichi Hiasa Minako Ishibashi Kisho Ohtani | 2004 | Circulation2004，109，8： | 1 |
| 9 | Quantum yields of active oxidative species formed on TiO2photocatalyst显示文摘 | ISHIBASHI KEN ICHI FUJISHIMA A WATANABE T | 2000 | Photochem and Photobiology A: Chemistry2000，，134： | 1 |
| 10 | Blocking of monocyte chemoattractant protein-1 (MCP-1) activity attenuates the severity of acute panereatitis in rats显示文摘 | TOSHIAKI ISHIBASHI HAIFENG ZHAO KEN KAWABE | 2008 | J GastroenteroI2008，43，： | 1 |
| 11 | Positive influence of aspirin on coronary endothelial function: Importance of the dose显示文摘AIM: To investigate the effects of different doses of aspirin on coronary endothelial function. METHODS: The study included 139 Japanese subjects(mean age, 60 years; 53 women) with angiographically normal coronary arteries. Patients were distributed into GroupⅠ(n = 63), who was administered aspirin and Group Ⅱ(n = 76), the control, who were not administered aspirin. GroupⅠwas further divided into GroupⅠa(n = 50, low-dose aspirin, 100 mg) and GroupⅠb(n = 13, high-dose aspirin, 500 mg). After a routine coronary angiography, acetylcholine(ACh; 3 and 30 μg/min successively) and nitroglycerin(NTG) were infused into the left coronary ostium over 2 min. The change in the diameter of the coronary artery in response to each drug was expressed as the percentage change from baseline values.RESULTS: The patient characteristics did not differ between the two groups. The change in coronary diameter in response to ACh was greater in GroupⅠthan in Group Ⅱ(P = 0.0043), although the NTG-induced coronary vasodilation was similar between groups. ACh-induced dilation was greater in GroupⅠa than in GroupⅠb(P = 0.0231). Multivariate regression analysis showed that a low-dose of aspirin(P = 0.0004) was one of the factors associated with ACh-induced dilation at 30 μg/min.CONCLUSION: In subjects with angiographically normal coronary arteries, aspirin only had a positive influence on coronary endothelial function at the low dose of 100 mg. This improvement of coronary endothelial function may be involved in the preventive effect of aspirin against future coronary events. | Hiroki Teragawa Naoya Mitsuba Ken Ishibashi Satoshi Kurisu Yasuki Kihara | 2013 | World Journal of Cardiology2013，5，11： | 1 |
| 12 | Preliminary results of a Japanese nationwide survey of neuroendocrine gastrointestinal tumors显示文摘 | Tetsuhide Ito Masao Tanaka Hironobu Sasano Yoshiyuki R. Osamura Iwao Sasaki Wataru Kimura Koji Takano Takao Obara Miyuki Ishibashi Kazuwa Nakao Ryuichiro Doi Akira Shimatsu Toshirou Nishida Izumi Komoto Yukio Hirata Masayuki Imamura Ken Kawabe Kazuhiko Na | 2007 | Journal of Gastroenterology2007，，6： | 1 |
| 13 | Quantum yields of active oxidative species formed on TiO2 photocatalyst 显示文摘 | KEN -ICHI ISHIBASHI AKIRA FUJISHIMA TOSHIYA WATANABE | 2000 | Photochem and Photobiology A : Chemistry2000，134，： | 1 |
| 14 | Blocking of monocyte chemoattractant protein-1 (MCP-1) activity attenuates the severity of acute pancreatitis in rats显示文摘 | Toshiaki Ishibashi Haifeng Zhao Ken Kawabe Takamasa Oono Kensuke Egashira Koichi Suzuki Hajime Nawata Ryoichi Takayanagi Tetsuhide Ito | 2008 | Journal of Gastroenterology2008，，1： | 1 |
| 15 | Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes in rat primary white adipocytes显示文摘 | Junetsu Ogasawara Tetsuya Izawa Takuya Sakurai Ken Shirato Yoshinaga Ishibashi Yoshinobu Ohira Hitoshi Ishida Hideki Ohno Takako Kizaki | 2015 | Biochemical and Biophysical Research Communicatio2015，，1： | 1 |
| 16 | An ex- tensively valid and stable method for derivation of all pa- rameters of a solar cell from a single current voltage char- acteristic显示文摘 | Ken ichi Ishibashi Yasuo Kimura Michio Niwano | 2008 | Journal of Applied Physics2008，103，09： | 1 |
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