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| 1 | 趋化因子CCL5在食管癌组织中的表达及意义显示文摘目的 探讨趋化因子CCL5在食管癌组织中的表达及意义.方法 采用逆转录聚合酶链反应(RT-PCR)检测食管癌组织和癌旁组织中CCL5、CD8和CD8^+T细胞杀伤功能相关细胞因子穿孔素(perforin)和颗粒酶B(granzyme B)的表达水平.采用流式细胞术检测食管癌患者外周血单核细胞(PBMC)和肿瘤浸润性淋巴细胞(TIL)中CD8^+T细胞和CCR5^+CD8^+T细胞比例.采用Transwell实验检测CCL5对T细胞运动的影响.结果 食管癌组织中CCL5和perforin mRNA的表达水平分别为0.348 2±0.300 1和0.181 9±0.118 6,癌旁组织中CCL5和perforin mRNA的表达水平分别为0.279 6±0.138 0和0.118 0±0.1098,但差异均无统计学意义(均P>0.05).食管癌组织中CD8和granzyme B mRNA的表达水平分别为0.4649±0.3008和0.6487±0.5160,癌旁组织中CD8和granzyme B mRNA的表达水平分别为0.279 0±0.173 4和0.469 7±0.259 1,差异有统计学意义(均P <0.05).食管癌患者中CCL5与CD8、perforin和granzyme B表达呈正相关(rCD8=0.272,P=0.034;rerforin=0.305,P=0.026;rgranzymeB=0.108,P=0.012).流式细胞术结果显示,TIL和PBMC中CD8^+T细胞比例分别为(45.86±16.09)%和(34.05±15.07)%,差异有统计学意义(P =0.022);TIL和PBMC中CCR5^+ CD8^+T细胞比例分别为(48.12±26.75)%和(19.53±13.67)%,差异有统计学意义(P<0.001).Transwell实验结果显示,CCL5显著增强T细胞趋化运动.CCL5表达与患者性别、年龄和淋巴结转移无关,但CCL5在早期食管癌组织中的相对表达水平为0.319 9±0.161 7,在晚期食管癌组织中的相对表达水平为0.232 8±0.121 0,差异有统计学意义(P=0.008).早期食管癌患者TIL中CD8^+T细胞和CCR5^+ CD8^+T细胞比例分别为(48.86±15.87)%和(56.23±26.23)%,而在晚期食管癌患者TIL中CD8^+T细胞和CCR5^+ CD8^+T细胞比例分别为(33.25±16.49)%和(33.53±21.03)%,差异均有统计学意义(PCD8 =0.007,PccR5=0.010).结论 CD8^+T细胞能够在CCL5的诱导下向肿瘤部位运动,并影响患者疾病进展,CCL5可作为食管癌治疗的新靶点。 | 刘金燕 李峰 陈新峰 王丽萍 岳冬丽 赵松 胡伟 Pawel Kalinski Stephen Thorne Hou Jingzhou 张毅 | 2014 | 中华肿瘤杂志2014，36，11： | 5 |
| 2 | Sister Mary Joseph's nodule as the first sign of pregnancy-associated gastric cancer:A case report显示文摘Sister Mary Joseph's nodule is an inconspicuous and uncommon clinical sign of advanced malignant disease, especially gastric cancer. Pregnancy-associated gastric cancer is an extremely rare condition and can be difficult to diagnose, due to the absence or misinterpretation of symptoms as pregnancy-related. Diagnostic aids, such as a basic chemistry panel and imaging techniques, may not show any abnormalities. We present a case of a 37-year-old pregnant patient whose umbilical nodule was the first presenting physical sign of gastric cancer, which had metastasized throughout the abdominal and pelvic regions. | Sara Fill Andrei Taran Hans-Ulrich Schulz Stefan Kahl Thomas Kalinski Bobbie Smith Serban-Dan Costa | 2008 | World Journal of Gastroenterology2008，14，6： | 3 |
| 3 | PIK3CA mutation asso- ciates with improved outcome in breast cancer 显示文摘 | Kalinsky K Jacks LM Heguy A | 2009 | Clin Cancer Res2009，15，16： | 1 |
| 4 | Induction of CD8 + T-cell responses against novel glioma associated antigen peptides and clinical activity by vaccinations with { alpha }-type 1 polarized dendritic cells and polyinosinic-polycytidylic acid stabilized by lysine and carboxymethylcellulose 显示文摘 | Okada H Kalinski P Ueda R | | 0，，03： | 1 |
| 5 | Lethal candida sepsis associated with myeloperoxidase deficiency and preeclampsia显示文摘 | Kalinski T Jentsch-Ullrich K Fill S | 2007 | APMIS2007，115，7： | 1 |
| 6 | Natural killer-dendritic cell cross-talk in cancer immunotherapy显示文摘 | Kalinski P Mailliard RB Giermasz A | 2005 | Expert Opin Biol Ther2005，5，10： | 1 |
| 7 | Molecular Cloning of a Protein Associated with Soybean Seed OilBodies That Is Similar to Thiol Proteases of the Papain Family 显示文摘 | Kalinski Jane M Weisemann | 1990 | The Journal of Biochemisty1990，265，13： | 1 |
| 8 | Estimating water content of soils from electrical resistivity 显示文摘 | Kalinski R J Kelly W E | 1993 | Geotechnical Testing Journal1993，16，3： | 1 |
| 9 | Helicobacter pylori encoding the pathogenicity island activates matrix metalloproteinase 1 in gastric epithelial cells via JNK and ERK显示文摘 | Krueger S Hundertmark T Kalinski T | 2006 | J Biol Chem2006，281，5： | 1 |
| 10 | IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells显示文摘 | Kalinski P Hilkens CMU Snijders A | 1997 | Immunology1997，159，1： | 1 |
| 11 | Electrical-resistivity measurements for evaluating compacted soil liners显示文摘 | KALINSKI R KELLY W | 1994 | Journal Geotechnology & Engineering ASCE1994，120，2： | 1 |
| 12 | Regulation of immune responses by prostaglandin E2 显示文摘 | Kalinski P | 2012 | J Immunol2012，188，1： | 1 |
| 13 | Dendritic cells incancer immunotherapy: vaccines or autologous transplants 显示文摘 | Kalinski P Edington H Zeh H J | 2011 | Immunol Res2011，50，23： | 1 |
| 14 | IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2,promote type 2 cytokine production in maturing human naive T helper cells显示文摘 | Hilkens CM Snijders A | 1997 | J Immunol1997，159，1： | 1 |
| 15 | 显示文摘 | Kalinski PK Hilkens CMU Wierenga EA | 1999 | Immunol Today1999，20，12： | 1 |
| 16 | Th1/Th2 balance in cancer,transplantation and pregnancy显示文摘 | Shurin M R Lu L Kalinski P | 1999 | Springer Semin Immunopathol1999，21，3： | 1 |
| 17 | Nonalcoholic fatty liver disease: biochemical and therapeutic considerations显示文摘 | Patrick-Melin AJ Kalinski MI Kelly KR | 2009 | Ukr Biokhim Zh2009，81，5： | 1 |
| 18 | PIK3CA mutation associates with improved outcome in breast cancer 显示文摘 | Kalinsky K Jacks LM Heguy A | 2009 | Clin Cancer Res2009，15，16： | 1 |
| 19 | T-cell priming by type-1 and type-2 polarized dentritic cells:the concept of a third signal显示文摘 | Kalinski P Hilkens CMU Wierenga EA | 1999 | Immunol Today1999，20，： | 1 |
| 20 | PIK3CA mutation associates with improved outcome in breast cance显示文摘 | Kalinsky K Jacks LM Heguy A | | 0，，16： | 1 |
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