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| 1 | Utility of tricalcium phosphate and osteogenic matrix cell sheet constructs for bone defect reconstruction显示文摘AIM: To determine the effects of transplanting osteogenic matrix cell sheets and beta-tricalcium phosphate(TCP) constructs on bone formation in bone defects.METHODS: Osteogenic matrix cell sheets were prepared from bone marrow stromal cells(BMSCs), and a porous TCP ceramic was used as a scaffold. Three experimental groups were prepared, comprised of TCP scaffolds(1) seeded with BMSCs;(2) wrapped with osteogenic matrix cell sheets; or(3) both. Constructs were implanted into a femoral defect model in rats and bone growth was evaluated by radiography, histology, biochemistry, and mechanical testing after 8 wk. RESULTS: In bone defects, constructs implanted with cell sheets showed callus formation with segmentalor continuous bone formation at 8 wk, in contrast to TCP seeded with BMSCs, which resulted in bone nonunion. Wrapping TCP constructs with osteogenic matrix cell sheets increased their osteogenic potential and resulting bone formation, compared with conventional bone tissue engineering TCP scaffolds seeded with BMSCs. The compressive stiffness(mean ± SD) values were 225.0 ± 95.7, 30.0 ± 11.5, and 26.3 ± 10.6 MPa for BMSC/TCP/Sheet constructs with continuous bone formation, BMSC/TCP/Sheet constructs with segmental bone formation, and BMSC/TCP constructs, respectively. The compressive stiffness of BMSC/TCP/Sheet constructs with continuous bone formation was significantly higher than those with segmental bone formation and BMSC/TCP constructs.CONCLUSION: This technique is an improvement over current methods, such as TCP substitution, and is useful for hard tissue reconstruction and inducing earlier bone union in defects. | Tomoyuki Ueha Manabu Akahane Takamasa Shimizu Yoshinobu Uchihara Yusuke Morita Naoya Nitta Akira Kido Yusuke Inagaki Kenji Kawate Yasuhito Tanaka | 2015 | World Journal of Stem Cells2015，7，5： | 7 |
| 2 | Bone regeneration with osteogenic matrix cell sheet and tricalcium phosphate:An experimental study in sheep显示文摘AIM To determine the effects of a cell sheet created from sheep bone marrow and tricalcium phosphate(TCP) on osteogenesis.METHODS Bone marrow cells were harvested from a sheep and cultured in a minimal essential medium(MEM) containing ascorbic acid phosphate(AscP) and dexamethasone(Dex). After 2 wk, the formed osteogenic matrix cell sheet was lifted from the culture dish using a scraper. Additionally, harvested bone marrow cells were cultured in MEM only as a negative control group, and in MEM with AscP, Dex, and β-glycerophosphate as a positive control group. For in vitro evaluation, we measured the alkaline phosphatase(ALP) activity and osteocalcin(OC) content in the media of the cultured cells from each group. For in vivo analysis, a porous TCP ceramic was used as a scaffold. We prepared an experimental group comprising TCP scaffolds wrapped with the osteogenic matrix cell sheets and a control group consisting of the TCP scaffold only. The constructs wereimplanted subcutaneously into athymic rats and the cell donor sheep, and bone formation was confirmed by histology after 4 wk.RESULTS In the in vitro part, the mean ALP activity was 0.39 ± 0.03 mg/well in the negative control group, 0.67 ± 0.04 mg/well in the sheet group, and 0.65 ± 0.07 mg/well in the positive control group. The mean OC levels were 1.46 ± 0.33 ng/well in the negative control group, 3.92 ± 0.16 ng/well in the sheet group, and 4.4 ± 0.47 ng/well in the positive control group, respectively. The ALP activity and OC levels were significantly higher in the cell sheet and positive control groups than in the negative control group(P < 0.05). There was no significant difference in ALP activity or OC levels between the cell sheet group and the positive control group(P > 0.05). TCP constructs wrapped with cell sheets prior to implantation showed bone formation, in contrast to TCP scaffolds alone, which exhibited poor bone formation when implanted, in the subcutaneous layer both in athymic rats and in the sheep. CONCLUSION This technique for preparing highly osteoinductive TCP may promote regeneration in large bone defects. | Tsutomu Kira Manabu Akahane Shohei Omokawa Takamasa Shimizu Kenji Kawate Tadanobu Onishi Yasuhito Tanaka | 2017 | World Journal of Orthopedics2017，8，10： | 2 |
| 3 | Oxidation resistance of Crr-AlxN and Til-xAlxN films显示文摘 | KAWATE M SUZUKI T KAWATEA M HASHIMOTOB A K SUZUKI T | 2003 | Surface and Coatings Technology2003，165，： | 1 |
| 4 | Glucocorticoid suppresses the canonical Wnt signal in cultured human osteoblasts显示文摘 | Keizo Ohnaka Mizuho Tanabe Hisaya Kawate | 2005 | Biochem Biophys Res Commun2005，329，1： | 1 |
| 5 | Tissue - engineered approach for the treatment of steroid - induced osteonecrosis of the femoral head:transplantation of autologous mesenchy- mal stem cells cultured with beta - tricaleium phosphate ce- ramics and free vaseularized fibula显示文摘 | Kawate K Yajima H Ohgushi H | 2006 | Artif Organs2006，30，12： | 1 |
| 6 | Crystal structure of the ATP-gated P2X4 ion channel in the closed state显示文摘 | KAWATE T MICHEL JC BIRDSONG WT | 2009 | Nature2009，460，7255： | 1 |
| 7 | Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants 显示文摘 | Kawate N | 2004 | J Reprod Dev2004，50，： | 1 |
| 8 | Cyclic testing of slab-column connections with shear reinforcement 显示文摘 | Robertson I N Kawat T Lee J | 2002 | ACI Structural Journal2002，99，5： | 1 |
| 9 | Hydrodynamics of a Solid - suspended Bubble Column with a Draught Tube Containing Low - density Particles显示文摘 | KAWATE | 1993 | Chem Eng Sci1993，48，： | 1 |
| 10 | DJ-1 protein expression as a predictor of pathological complete remission after neoadjuvant chemotherapy in breast cancer patients显示文摘 | Kawate T Iwaya K Kikuchi R | 2013 | Breast Cancer Res Treat2013，139，1： | 1 |
| 11 | Rare multiple combined anomaly of the vertebral vessels and bronchial artery 显示文摘 | Kawate T Futamata H Kitami Y | 2008 | #mat Sci Int2008，83，4： | 1 |
| 12 | Perioperative reabtime monitoring of indocyanine green clearance by pulse spectrophotometry predicts remnant liver functional reserve in resection of hepatocellular carcinoma 显示文摘 | Ohwada S Kawate S Hamada K | 2006 | Br J Surg2006，93，3： | 1 |
| 13 | Detection of transforming growth factor-a and epidermal growth factor receptor mRNA and immunohistochemical localization of their proteins in the ovine uterus during the early implantation period显示文摘 | Tsubutani D Kawate N | 2002 | Histochem J2002，34，： | 1 |
| 14 | Indications for free vascu- larized fibular grafting for the treatment of osteonecrosis of the femoral head显示文摘 | Kawate K Yajima H Sugimoto K | 2007 | BMC Musculoskelet Disord2007，8，： | 1 |
| 15 | Oxidation resistance of Cr1-xAlx N and Ti1-xAlxN films显示文摘 | Kawate M Hashimoto A K Suzuki T | | 0，，02： | 1 |
| 16 | Perioperative real-time monitoring of in- docyanine green clearance by pulse spec- trophotometry predicts remnant liver functional reserve in resection of hepatoc- ellular carcinoma显示文摘 | Ohwada S Kawate S Hamada K | 2006 | Br J Surg2006，93，： | 1 |
| 17 | Left renal vein graft for vascular reconstruction in abdominal malignancy 显示文摘 | Ohwada S Hamada K Kawate S | 2007 | World J Surg2007，31，6： | 1 |
| 18 | Insulin-like peptide 3 stimulates testosterone secretion in mouse Leydig cells via cAMP pathway显示文摘 | Pathirana IN Kawate N Bullesbach EE | 2012 | Regul Pep t2012，178，123： | 1 |
| 19 | Glucocorticoid suppresses the canonical Wnt signal in cultured human osteoblasts显示文摘 | Ohnaka K Tanabe M Kawate H | | 0，，： | 1 |
| 20 | Perioperative real - timemonitoring of indocyanine green clearance by pulse spectrophotom-etry predicts remnant liver functional reserve in resection of hepato-cellular carcinoma显示文摘 | Ohwada S Kawate S Hamada K | 2006 | Br J Surg2006，93，3： | 1 |
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