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| 1 | Combination of Cyclamen persicum Mill. floral gene promoters and chimeric repressors for the modification of ornamental traits in Torenia fournieri Lind.显示文摘Although chimeric repressors such as the Arabidopsis TCP3 repressor are known to have significant effects on flower morphology and color,their cellular-level effects on flower petals are not understood.The promoter sequences of the genes expressed in the flowers of cyclamen,a representative potted flower grown during the winter season,are also unknown.Here,we isolated eight promoters from cyclamen genes that are reportedly expressed in the petals.These promoters were then fused to four chimeric repressors and introduced into the model flower torenia to screen for effective combinations of promoters and repressors for flower breeding.As expected,some of the constructs altered flower phenotypes upon transformation.We further analyzed the effects of chimeric repressors at the cellular level.We observed that complicated petal and leaf serrations were accompanied by excessive vascular branching.Dichromatism in purple anthocyanin was inferred to result in bluish flowers,and imbalanced cell proliferation appeared to result in epinastic flowers.Thus,the genetic constructs and phenotypic changes described in this report will benefit the future breeding and characterization of ornamental flowers. | Ichiro Kasajima Norihiro Ohtsubo Katsutomo Sasaki | 2017 | Horticulture Research2017，4，1： | 2 |
| 2 | Virus-induced gene silencing and virusinduced flowering in strawberry(Fragaria×ananassa)using apple latent spherical virus vectors显示文摘Apple latent spherical virus(ALSV)vector is a convenient alternative to genetic transformation in horticultural plants,especially in species recalcitrant to genetic transformation.ALSV,an RNA virus,can infect a wide variety of plant species including major horticultural plants without inducing symptoms.Here,methodologies were developed for infection of ALSV vectors to strawberry seedlings and plantlets cultured in vitro.A seed-propagated F1 hybrid strawberry cultivar'Yotsuboshi'was aseptically grown on half-strength Murashige–Skoog medium for 1 month and true leaves were inoculated with an ALSV RNA preparation by particle bombardment.ALSV vector infection rates varied from 58 to 100%according to the insertion sequences,in‘Yotsuboshi’seedlings.Plantlets(‘Dover’)propagated in vitro could also be infected with ALSV vector at a similar infection rate.For virus-induced gene silencing(VIGS),we prepared an ALSV vector carrying a 201 nucleotide segment of the strawberry phytoene desaturase gene.‘Yotsuboshi’and‘Dover’plants infected by this vector generated completely white leaves at fifth or sixth true leaves and above.For virus-induced flowering(VIF),we used an ALSV vector expressing the Arabidopsis thaliana flowering locus T gene.Strawberry seedlings infected by this vector started to flower from about 2 months post inoculation and bore fruits with viable seeds.The ALSV vector was no longer detected in any of the seedlings from early-flowered strawberries.Thus,the ALSV vector may be beneficial for examination of gene functions by VIGS in strawberry,and VIF using ALSV vector constitutes an effective new plant breeding technique for the promotion of cross-breeding in strawberry. | Chunjiang Li Noriko Yamagishi Ichiro Kasajima Nobuyuki Yoshikawa | 2019 | Horticulture Research2019，6，1： | 2 |
| 3 | Hypoxia Induces Apoptosis With Enhanced Expression of Fas Antigen Messenger RNA in Cultured Neonatal Rat Cardiomyocytes显示文摘 | Masato Tanaka Hiroshi Ito Susumu Adachi Hajime Akimoto Toshio Nishikawa Takeshi Kasajima Fumiaki Marumo Michiaki Hiroe | 1994 | Circulation Research1994，，3： | 1 |
| 4 | Differential expression of his- tone deacetylases HDAC1,2 and 3 in human breast cancer--overex- pression of HDAC2 and HDAC3 is associated with clinicopathological indicators of disease progression 显示文摘 | Mtlller BM Jana L Kasajima A | 2013 | BMC Cancer2013，13，： | 1 |
| 5 | Enhanced in situ expression of aldose reductase in peripheral nerve and renal glomeruli in diabetic patients显示文摘 | Kasajima H Yamagishi S Sugai S | 2001 | Virchows Arch2001，439，： | 1 |
| 6 | Increased in situ expression of nitric oxide synthase inhuman colorectal cancer 显示文摘 | Yagihashi N Kasajima H Sugai S | 2001 | Virchows Arch2001，436，2： | 1 |
| 7 | Role of eabbeling in water densieation in the Greenland Basin显示文摘 | KASAJIMA Y JOHANNESSEN T | 2009 | Ocean Sci2009，5，： | 1 |
| 8 | Assessment of potentialindigenous plant species for the phytoremediation of arsenic-con-taminated areas of bangladesh 显示文摘 | Mahmuc R Inone N Kasajimas | 2008 | International Journal of Phy-toremediation2008，10，2： | 1 |
| 9 | Increased in situ expression of nitric oxide synthase in human colorectal cancer显示文摘 | Yagihashi N Kasajima H Sugai S | 2000 | Virchows Arch2000，436，2： | 1 |
| 10 | Assessment of potential indigenous plant species for the phytoremediation of arsenic-contaminated areas of Bangladesh 显示文摘 | Mahmud R Inoue N Kasajima S Y | 2008 | Inter J Phytoremediation2008，10，2： | 1 |
| 11 | Assessment of potential indigenous plant species for the Phytoremediation of Arsenic-contaminated areas of Bangladesh显示文摘 | Mahmud R Inoue N Kasajima SY | 2008 | International Journal of Phytoremediation2008，10，2： | 1 |
| 12 | Increased in situ expression of nitric oxide synthase in human colorectal cancer 显示文摘 | Yagihashi N Kasajima H Sugai S | 2000 | Virchows Arch2000，436，2： | 1 |
| 13 | A protocol for rapid DNA extraction from Arabidopsis thaliana for PCR analysis显示文摘 | Kasajima I Ide Y Ohkama-Ohtsu N | 2004 | Plant Mol Biol Rep2004，，22： | 1 |
| 14 | Toxic hepatitis induced by show-wu-pian, a Chinese herbal preparation显示文摘 | FURUKAWA M KASAJIMA S NAKAMURA Y | 2010 | Intern Med2010，49，15： | 1 |
| 15 | Aplastic anema associated with systemic lupus erythematosus 显示文摘 | Sumimoto S Kawai M Kasajima Y | 1991 | Am J Hematol1991，38，4： | 1 |
| 16 | New trends of immunohistochemistry for making differential diagnosis of breast lesions显示文摘 | Moriya T Kasajima A Ishida K | 2006 | Med Mol Morphol2006，39，1： | 1 |
| 17 | Systematic e- valuation of the miRNA-ome and its downstream effects on mRNA expression identifies gastric cancer progression 显示文摘 | Tchernitsa O Kasajima A Schafer R | 2010 | J Pathol2010，222，3： | 1 |
| 18 | Case reportsof primary pulmonary adenocarcinoma with pleuralspread : So-called pseudomesotheliomatous adenocarcino-ma 显示文摘 | Saito R Kasajima A Taniuchi S | 2012 | Pathology International2012，62，10： | 1 |
| 19 | Increased in situ expression of nitric oxide synthase in human colorectal cancer显示文摘 | Yagihashi N Kasajima H Sugai S | 2000 | Virehows Arch2000，436，2： | 1 |
| 20 | Urinary excretion of anthocyanins in humans after cranberry juice ingestion显示文摘 | Ohnishi R Ito H Kasajima N | 2006 | Biosci Biotechnol Biochem2006，70，7： | 1 |
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