| 2 | 用改装的Super-Spinner搅拌瓶连续灌流培养产凝血酶原CHO工程细胞显示文摘A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfused cultivation of a recombinant CHO cell line,CHO2DS producing prothrombin was performed with the perfusion of a protein\|free medium DF6S.The cell retention rate was more than 90% during the 24 days continuously perfused cultivation.The viable cell density of CHO2DS and prothrombin concentration reached 4.62×10 6(cells.m/L) and 11.3(mg/L)respectively after 9 days culture. | 陈昭烈 Kai Iding Dirk Lütkemeyer JürgenLehmann | 2001 | 生物工程学报2001，17，1： | 0 |
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