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| 1 | A novel integrated step-feed biofilm process for the treatment of decentralized domestic wastewater in rural areas of China显示文摘For wastewater treatment in rural areas, a novel three-stage step-feed wastewater treatment system, combined with a drop-aeration biofilm process, was tested in the laboratory to investigate its performance in removing suspended solids (SS), chemical oxygen demand (COD), NH4+-N, total nitrogen (TN), and total phosphorus (TP). The removal rates of SS, COD and NH4+-N were 90%, 80%, and 90% in efluent concentrations less than 10 mg/L, 50 mg/L and 8 mg/L, respectively. The TP removal rate was less satisfactory. The C/N ratio in the raw wastewater was often less than 3.5, and the removal efficiency of TN was therefore limited. A carbon-release batch experiment was carried out to measure the feasibility of enhancing denitrification at low influent C/N ratios. The result showed that the C/N could be over 9.0 in the supernatant. Polymerase chain reaction denaturing gradient gel electrophoresis technology was used to reveal the changes in the bacterial community during different stages of the integrated step-feed biofilm process. The results showed that banding patterns and the distribution of dominant bands for the same experimental period in different aerobic zones were similar. Phylogenetic analysis indicated that lanes 10, 11 and 12, which presented three aerobic zones at the same operation period, had the closest phylogenetic relationship among the lanes. | Hanwen Liang, Min Gao, Junxin Liu, Yuansong Wei, Xuesong Guo Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. | 2010 | Journal of Environmental Sciences2010，22，3： | 28 |
| 2 | Photodynamic therapy guidelines for the management of oral leucoplakia显示文摘With recent developments in photosensitizers and light delivery systems,topical 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has become the fourth alternative therapeutic approach in the management of oral leucoplakia (OLK) due to its minimally invasive nature,efficacy,and low risk of systemic side effects and disfigurement.This report presents step-by-step guidelines for applying topical ALA-PDT in the management of OLK based on both the clinical experience of the authors and a systematic review of the current literature.Studies using protocols with standardized parameters and randomized clinical trials at multiple centres with adequate sample sizes and both interim and long-term follow-ups are needed before universally applicable guidelines can be produced in this field. | Qianming Chen Hongxia Dan Fan Tang Jiongke Wang Xiaoying Li Junxin Cheng Hang Zhao Xin Zeng | 2019 | International Journal of Oral Science2019，11，2： | 18 |
| 3 | Characteristics of greenhouse gas emission in three full-scale wastewater treatment processes显示文摘Three full-scale wastewater treatment processes, Orbal oxidation ditch, anoxic/anaerobic/aerobic(reversed A2O) and anaerobic/anoxic/aerobic(A2O), were selected to investigate the emission characteristics of greenhouse gases(GHG), including carbon dioxide(CO2), methane(CH4) and nitrous oxide(N2O). Results showed that although the processes were different, the units presenting high GHG emission fluxes were remarkably similar, namely the highest CO2 and N2O emission fluxes occurred in the aerobic areas, and the highest CH4 emission fluxes occurred in the grit tanks. The GHG emission amount of each unit can be calculated from its area and GHG emission flux. The calculation results revealed that the maximum emission amounts of CO2, CH4 and N2O in the three wastewater treatment processes appeared in the aerobic areas in all cases. Theoretically, CH4 should be produced in anaerobic conditions, rather than aerobic conditions. However, results in this study showed that the CH4 emission fluxes in the forepart of the aerobic area were distinctly higher than in the anaerobic area. The situation for N2O was similar to that of CH4: the N2O emission flux in the aerobic area was also higher than that in the anoxic area. Through analysis of the GHG mass balance, it was found that the flow of dissolved GHG in the wastewater treatment processes and aerators may be the main reason for this phenomenon. Based on the monitoring and calculation results, GHG emission factors for the three wastewater treatment processes were determined. The A2O process had the highest CO2 emission factor of 319.3 g CO2/kg CODremoved, and the highest CH4 and N2O emission factors of 3.3 g CH4/kg CODremovedand 3.6 g N2O/kg TNremovedwere observed in the Orbal oxidation ditch process. | Xu Yan Lin Li Junxin Liu | 2014 | Journal of Environmental Sciences2014，26，2： | 17 |
| 4 | Biodegradation of malathion by Acinetobacter johnsonii MA19 and optimization of cometabolism substrates显示文摘To enhance the removal effciency of malathion in the wastewater from organophosphate pesticide mill,a bacterium,Acinetobacter johnsonii MA19,that could degrade malathion with cometabolism was isolated from malathion-polluted soil samples using enrichment culture techniques. Four kinds of additional compounds,sodium succinate,sodium acetate,glucose,and fructose were tested to choose a favorite carbon source for the cometabolism of strain MA19. The results showed that sodium succinate and sodium acetate could promote malathion biodegradation and cell growth. The investigation results of the effects of sodium succinate concentrations on the malathion biodegradation indicated that the more sodium succinate supplied resulted in quick degradation of malathion and fast cells multiplied. Zero-order kinetic model was appropriate to describe the malathion biodegradation when the concentration of sodium succinate was more than 0.5144 g/L. The degradation rate constant(K) reached the maximum value of 3.5837 mg/(L·h) when the mass ratio of sodium succinate to malathion was 128.6 mg/mg. The aquatic toxicity of the malathion was evaluated using the test organism,Limnodrilus hoffmeisteri. The data obtained suggested that the toxicity of malathion could be ignored after 84 h biodegradation. Our result demonstrates the potential for using bacterium A. johnsonii MA19 for malathion biodegradation and environmental bioremediation when some suitable conventional carbon sources are supplied. | XIE Shan LIU Junxin LI Lin QIAO Chuanling | 2009 | Journal of Environmental Sciences2009，21，1： | 16 |
| 5 | Decentralized wastewater treatment technologies and management in Chinese villages显示文摘在中国在农村区域对待污水的问题在最近的年里吸引了增加的注意。然而,仅仅,有的 6% 农村区域在 2010 底喷洒排水和废水处理系统。估计当前使用的技术是必要的以便在农村区域的污水处理能适当地被开发,到提供可靠、买得起的废水处理,在这些区域。我们评估了在各一直在操作至少一年了的 62 台设备上进行案例研究当前在农村区域使用的污水处理技术。我们的学习在 2009 和 2011 之间被执行,并且目的是在那经期期间估计状况并且估计在村庄里涉及分散的污水治疗的任何问题。我们发现那个分散的污水处理是村庄里的最流行的废水处理方法,并且最普通的主要处理技术在农村区域使用了,这是化粪池。我们的调查加亮需要为估计自河质量建立标准,包括目标污染物质的一个范围。我们的结果也证明自河应该被再使用在不同区域满足本地环境要求,特别在生态地敏感的区域。 | Xuesong GUO Zehang LIU Meixue CHEN Junxin LIU Min YANG | 2014 | Frontiers of Environmental Science & Engineering2014，8，6： | 13 |
| 6 | An innovative integrated system utilizing solar energy as power for the treatment of decentralized wastewater显示文摘This article reports an innovative integrated system utilizing solar energy as power for decentralized wastewater treatment, which consists of an oxidation ditch with double channels and a photovoltaic (PV) system without a storage battery. Because the system operates without a storage battery, which can reduce the cost of the PV system, the solar radiation intensity affects the amount of power output from the PV system. To ensure that the power output is sufficient in all different weather conditions, the solar radiation intensity of 78 W/m 2 with 95% confidence interval was defined as a threshold of power output for the PV system according to the monitoring results in this study, and a step power output mode was used to utilize the solar energy as well as possible. The oxidation ditch driven by the PV system without storage battery ran during the day and stopped at night. Therefore, anaerobic, anoxic and aerobic conditions could periodically appear in the oxidation ditch, which was favorable to nitrogen and phosphate removal from the wastewater. The experimental results showed that the system was efficient, achieving average removal efficiencies of 88% COD, 98% NH 4 + -N, 70% TN and 83% TP, under the loading rates of 140 mg COD/(g MLSS·day), 32 mg NH 4 + -N/(g MLSS·day), 44 mg TN/(g MLSS·day) and 5 mg TP/(g MLSS·day). | Changfu Han Junxin Liu Hanwen Liang Xuesong Guo Lin Li | 2013 | Journal of Environmental Sciences2013，25，2： | 12 |
| 7 | Characterization and source analysis of indoor/outdoor culturable airborne bacteria in a municipal wastewater treatment plant显示文摘The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoor bacteria. In this work, the characterization of airborne bacteria was studied in a municipal wastewater treatment plant in Beijing, China. Two indoor(i.e., fine screen room and sludge dewatering house) and two outdoor(i.e., aeration tank and control site) sampling sites were selected. An Andersen six-stage impactor was used for collecting culturable airborne bacteria in the air, and Illumina MiSeq sequencing was conducted to track the emission source of the culturable airborne bacteria. The results indicate that, compared with the outdoor aeration tank site, the concentrations of culturable airborne bacteria in the indoor fine screen room with poor ventilation were more than ten times higher and the particle size was about twice as large. The community structures of indoor and outdoor culturable airborne bacteria were obviously different. Enterobacteriaceae and opportunistic pathogens were detected in indoor culturable airborne bacteria, with wastewater and sludge dewatering machine identified as the primary sources. Conversely,Enterobacteriaceae and opportunistic pathogens were not detected in outdoor culturable airborne bacteria. Outdoor high wind speed might have resulted in rapid dilution and mixing of culturable airborne bacteria generated from the aeration tank with the ambient air. The results of the present research suggest that covering pollution sources, increasing ventilation rates, and using protective measures for personnel should be implemented to decrease the exposure risk to indoor culturable airborne bacteria. | Guangsu Xu Yunping Han Lin Li Junxin Liu | 2018 | Journal of Environmental Sciences2018，30，12： | 12 |
| 8 | Rapid detection of multiple class pharmaceuticals in both municipal wastewater and sludge with ultra high performance liquid chromatography tandem mass spectrometry显示文摘This work described the development,optimization and validation of an analytical method for rapid detection of multiple-class pharmaceuticals in both municipal wastewater and sludge samples based on ultrasonic solvent extraction,solid-phase extraction,and ultra high performance liquid chromatography–tandem mass spectrometry quantification.The results indicated that the developed method could effectively extract all the target pharmaceuticals(25)in a single process and analyze them within 24 min.The recoveries of the target pharmaceuticals were in the range of 69%–131%for wastewater and 54%–130%for sludge at different spiked concentration levels.The method quantification limits in wastewater and sludge ranged from 0.02 to 0.73 ng/L and from 0.02 to 1.00μg/kg,respectively.Subsequently,this method was validated and applied for residual pharmaceutical analysis in a wastewater treatment plant located in Beijing,China.All the target pharmaceuticals were detected in the influent samples with concentrations varying from0.09 ng/L(tiamulin)to 15.24μg/L(caffeine);meanwhile,up to 23 pharmaceuticals were detected in sludge samples with concentrations varying from 60 ng/kg(sulfamethizole)to8.55 mg/kg(ofloxacin).The developed method demonstrated its selectivity,sensitivity,and reliability for detecting multiple-class pharmaceuticals in complex matrices such as municipal wastewater and sludge. | Xiangjuan Yuan Zhimin Qiang Weiwei Ben Bing Zhu Junxin Liu | 2014 | Journal of Environmental Sciences2014，26，9： | 12 |
| 9 | Characterization of the airborne bacteria community at different distances from the rotating brushes in a wastewater treatment plant by 16S rRNA gene clone libraries显示文摘Biological risks of bioaerosols emitted from wastewater treatment processes have attracted wide attention in the recent years. However, the culture-based analysis method has been mostly adopted for detecting the bacterial community in bioaerosols, which may result in the underestimation of total microorganism concentration as not all microorganisms are cultivable. In this study, oligonucleotide fingerprinting of 16S rRNA genes was applied to reveal the composition and structure of the bacterial community in bioaerosols from an Orbal oxidation ditch in a Beijing wastewater treatment plant (WWTP). Bioaerosols were collected at different distances from the aerosol source, rotating brushes, and the sampling height was 1.5 m which is the common respiratory height of a human being. The bacterial communities of bioaerosols were diverse, and the lowest bacterial diversity was found at the sampling site just after the rotating brush rotating brush. A large proportion of bacteria in bioaerosols were affiliated with Proteobacteria and Bacteroidetes. Numerous bacteria present in the bioaerosols also emerged in water, indicating that the bacterial community in the bioaerosols was related to that of the aerosols' sources. The forced aeration of rotating brushes brought about observably distinct bacterial communities between sampling sites situated before and after the rotating brush. Isolation sources of closest relatives in bioaerosols clone libraries were associated with the aqueous environment in the WWTP. Common potential pathogens in bioaerosols as well as those not reported in previous research were also analyzed in this study. Measures should be adopted to reduce the emission of bioaerosols and prevent their exposure to workers. | Yunping Han Lin Li Junxin Liu | 2013 | Journal of Environmental Sciences2013，25，1： | 12 |
| 10 | Effect of sludge retention time on sludge characteristics and membrane fouling of membrane bioreactor显示文摘Three identical membrane bioreactors (MBRs) were operated over 2 years at different sludge retention time (SRT) of 10 d, 40 d and no sludge withdrawal (NS), to elucidate and quantify the effect of SRT on the sludge characteristics and membrane fouling. The hydraulic retention times of these MBRs were controlled at 12 h. With increasing SRT, the sludge concentrations in the MBRs increased, whereas the ratio of volatile suspended solid to the total solid decreased, and the size of sludge granule diminished in the meantime. A higher sludge concentration at long SRT could maintain a better organic removal effciency, and a longer SRT was propitious to the growth of nitrifiers. The performance of these MBRs for the removal of COD and NH4+-N did not change much with different SRTs. However, the bioactivity decreased as SRT increase. The measurement of specific oxygen uptake rates (SOUR) and fiuorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes testified that SOUR and the proportion of the bacteria-specific probe EUB338 in all DAPI-stainable bacteria decreased with increasing SRT. The concentrations of total organic carbon, protein, polysaccharides and soluble extracellular polymeric substance (EPS) in the mixed liquor supernatant also decreased with increasing SRT. The membrane fouling rate was higher at shorter SRT, and the highest fouling rate appeared at a SRT of 10 d. Both the sludge cake layer and gel layer had contribution to the fouling resistance, but the relative contribution of the gel layer decreased as SRT increase. | OUYANG Ke LIU Junxin | 2009 | Journal of Environmental Sciences2009，21，10： | 10 |
| 11 | Removal of airborne microorganisms emitted from a wastewater treatment oxidation ditch by adsorption on activated carbon显示文摘Bioaerosol emissions from wastewater and wastewater treatment processes are a significant subgroup of atmospheric aerosols. Most previous work has focused on the evaluation of their biological risks. In this study, however, the adsorption method was applied to reduce airborne microorganisms generated from a pilot scale wastewater treatment facility with oxidation ditch. Results showed adsorption on granule activated carbon (GAC) was an efficient method for the purification of airborne microorganisms. The GAC itself had a maximum adsorption capacity of 2217 CFU/g for airborne bacteria and 225 CFU/g for fungi with a flow rate of 1.50 m 3 /hr. Over 85% of airborne bacteria and fungi emitted from the oxidation ditch were adsorbed within 80 hr of continuous operation mode. Most of them had a particle size of 0.65-4.7 μm. Those airborne microorganisms with small particle size were apt to be adsorbed. The SEM/EDAX, BET and Boehm's titration methods were applied to analyse the physicochemical characteristics of the GAC. Relationships between GAC surface characteristics and its adsorption performance demonstrated that porous structure, large surface area, and hydrophobicity rendered GAC an effective absorber of airborne microorganisms. Two regenerate methods, ultraviolet irradiation and high pressure vapor, were compared for the regeneration of used activated carbon. High pressure vapor was an effective technique as it totally destroyed the microorganisms adhered to the activated carbon. Microscopic observation was also carried out to investigate original and used adsorbents. | Lin Li Min Gao Junxin Liu Xuesong Guo | 2011 | Journal of Environmental Sciences2011，23，5： | 10 |
| 12 | Advanced hydrogels for the repair of cartilage defects and regeneration显示文摘Cartilage defects are one of the most common symptoms of osteoarthritis(OA),a degenerative disease that affects millions of people world-wide and places a significant socio-economic burden on society.Hydrogels,which are a class of biomaterials that are elastic,and display smooth surfaces while exhibiting high water content,are promising candidates for cartilage regeneration.In recent years,various kinds of hydrogels have been developed and applied for the repair of cartilage defects in vitro or in vivo,some of which are hopeful to enter clinical trials.In this review,recent research findings and developments of hydrogels for cartilage defects repair are summarized.We discuss the principle of cartilage regeneration,and outline the requirements that have to be fulfilled for the deployment of hydrogels for medical applications.We also highlight the development of advanced hydrogels with tailored properties for different kinds of cartilage defects to meet the requirements of cartilage tissue engineering and precision medicine. | Wei Wei Yuanzhu Ma Xudong Yao Wenyan Zhou Xiaozhao Wang Chenglin Li Junxin Lin Qiulin He Sebastian Leptihn Hongwei Ouyang | 2021 | Bioactive Materials2021，6，4： | 8 |
| 13 | Sulfur dioxide and o-xylene co-treatment in biofilter:Performance, bacterial populations and bioaerosols emissions显示文摘Sulfur dioxide(SO_2) and benzene homologs are frequently present in the off-gas during the process of sewage sludge drying. A laboratory scale biofilter was set up to co-treat SO_2 and o-xylene in the present study. SO_2 and o-xylene could be removed simultaneously in a single biofilter. Their concentration ratio in the inlet stream influenced the removal efficiencies. It is worth noting that the removal of SO_2 could be enhanced when low concentrations of o-xylene were introduced into the biofilter. Pseudomonas sp., Paenibacillus sp., and Bacillus sp. were the main functional bacteria groups in the biofilter. Sulfur-oxidizing bacteria(SOB) and o-xylene-degrading bacteria(XB) thrived in the biofilter and their counts as well as their growth rate increased with the increase in amount of SO_2 and o-xylene supplied. The microbial populations differed in counts and species due to the properties and components of the compounds being treated in the biofilter. The presence of mixed substrates enhanced the diversity of the microbial population. During the treatment process, bioaerosols including potentially pathogenic bacteria, e.g., Acinetobacter lwoffii and Aeromonas sp., were emitted from the biofilter. Further investigation is needed to focus on the potential hazards caused by the bioaerosols emitted from waste gas treatment bioreactors. | Yongli Sun Song Xue Lin Li Wenjie Ding Junxin Liu Yunping Han | 2018 | Journal of Environmental Sciences2018，30，7： | 7 |
| 14 | Occurrence and removal of organic micropollutants in the treatment of landfill leachate by combined anaerobic-membrane bioreactor technology显示文摘Organic micropollutants,with high toxicity and environmental concern,are present in the landfill leachate at much lower levels than total organic constituents (chemical oxygen demand (COD),biochemical oxygen demand (BOD),or total organic carbon (TOC)),and few has been known for their behaviors in different treatment processes.In this study,occurrence and removal of 17 organochlorine pesticides (OCPs),16 polycyclic aromatic hydrocarbons (PAHs),and technical 4-nonylphenol (4-NP) in landfill leachate in a combined anaerobic-membrane bioreactor (MBR) were investigated.Chemical analyses were performed in leachates sampled from different treatment processes,using solid-phase extraction and gas chromatography with electron capture detector and mass spectrometry.Concentrations of OCPs,PAHs,and 4-NP in the raw leachate were detected within the range from ND (not detected) to 595.2 ng/L,which were as low as only 10-7-10-5 percentage of TOC (at the concentration of 2,962 mg/L).The removal of 4-NP was mainly established in the MBR process,in agreement with removals of COD,BOD,and TOC.However,the removals of OCPs and PAHs were different,mainly achieved in the anaerobic process.High removal effciencies of both total organic constituents and organic micropollutants could be achieved by the combined anaerobic-MBR technology.The removal effciencies of total organic constituents were in the order of BOD (99%) > COD (89%) > TOC (87%),whereas the removal effciencies of investigated organic micropollutants were as follows: OCPs (94%) > 4-NP (77%) > PAHs (59%). | Yiping XU Zijian WANG | 2008 | Journal of Environmental Sciences2008，20，11： | 7 |
| 15 | Investigation on mechanism of phosphate removal on carbonized sludge adsorbent显示文摘For the removal of phosphate(PO_4^(3-)) from water, an adsorbent was prepared via carbonization of sewage sludge from a wastewater treatment plant: carbonized sludge adsorbent(CSA). The mechanism of phosphate removal was determined after studying the structure and chemical properties of the CSA and its influence on phosphate removal. The results demonstrate that phosphate adsorption by the CSA can be fitted with the pseudo second-order kinetics and Langmuir isotherm models, indicating that the adsorption is single molecular layer adsorption dominated by chemical reaction. The active sites binding phosphate on the surface are composed of mineral particles containing Si/Ca/Al/Fe. The mineral containing Ca, calcite, is the main factor responsible for phosphate removal. The phosphate removal mechanism is a complex process including crystallization via the interaction between Ca^(2+) and PO_4^(3-); formation of precipitates of Ca^(2+), Al^(3+), and PO_4^(3-); and adsorption of PO_4^(3-)on some recalcitrant oxides composed of Si/Al/Fe. | Lei Zhang Junxin Liu Xuesong Guo | 2018 | Journal of Environmental Sciences2018，30，2： | 7 |
| 16 | Evaluation of phosphorus removal from wastewater by soils in rural areas in China显示文摘Low-cost, easy-maintenance and high-efficiency decentralized wastewater treatment technologies are urgently needed in rural areas of China. Processes with high potential for phosphorus removal are of great interest. However, commonly used treatment methods often do not meet the strict criteria for removing phosphorus from rural wastewater. In order to search an economic and simple technology for phosphorus removal from the common bio-technologies efluent, seven soil types collected from different rural areas in China were investigated for their ability to remove phosphorus. X-ray diffraction (XRD) was used to analyse the mineral structure, and inductively coupled plasma optical emission spectrometer (ICP-OES) was used to analyse the geochemical composition of the soil samples. Three primary minerals-quartz, albite and montmorillonite-were clearly detected. The samples were divided into two soil types, acidic soils and alkaline soils, based on their pH values. The geochemical composition study indicated that a higher percentage of Ca and Mg occurred in alkaline soils (pH>8) than in acidic soils (pH<6.5). Adsorption isotherms from batch experiments fitted the Langmuir and Freundlich models well, the maximum P adsorption capacities ranged from 0.256 to 1.598 mg P/g, indicating a high phosphorus removal potential for all of these soils. The P fractions extracted revealed that the sum of NaOH-extracted inorganic P (NaOH-Pi) was the major P component in the acidic soils, and CaCO3-bound phosphorus (Ca-P) in the alkaline soils. Dynamic adsorption simulation showed that these soils have the ability to remove phosphorus from wastewater. | Hanwen Liang, Junxin Liu, Yuansong Wei, Xuesong GuoResearch Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China | 2010 | Journal of Environmental Sciences2010，22，1： | 7 |
| 17 | Landfill leachate treatment by MBR: Performance and molecular weight distribution of organic contaminant显示文摘A membrane bioreactor (MBR) with an air-lift bioreactor and gravity flow is applied to treating landfill leachate. More than 99% of BOD5 (biochemical oxygen demand for five days) removal effi-ciency is achieved with less than 35 mg/L of BOD_5 in the effluent at less than 1.71 kg BOD5/m^3·d of BOD_5 loading rate. When DO (dissolved oxygen) is maintained at the range of 2.3―2.8 mg/L and the loading rate of NH_4^+-N (ammonium nitrogen) is kept at 0.16― 0.24 kg NH_4^+-N/m^3·d, the NH_4^+-N in the effluent is less than 15 mg/L. However, compared with high removal rates of BOD_5 and NH_4^+-N, the removal effi-ciency of soluble chemical oxygen demand (SCOD) varies between 70% and 96%. The investigation of molecular weight (MW) distribution has been carried out by the gel permeation chromatography (GPC) so as to understand the fate of organic matters in the MBR treating of landfill leachate. Results indicate that organic matters of the landfill leachate are composed of a high MW fraction (MW of the peak, MWp = 11480―13182 Da) and a low MW fraction (MWp = 158―275 Da). The high MW fraction is not biode-gradable, but can be decreased with microfiltration membrane. The most of the low MW fraction is bio-degradable, but the residue of the low MW fraction is able to permeate through the membrane, thus re-sulting in high SCOD in the effluent of the MBR. | CHEN Shaohua LIU Junxin | 2006 | Chinese Science Bulletin2006，51，23： | 7 |
| 18 | Effects of various pretreatments on biohydrogen production from sewage sludge显示文摘The sewage sludge of wastewater treatment plant is a kind of biomass which contains many organics, mainly carbohydrates and proteins. Four pretreatments, acid pretreatment, alkaline pretreatment, thermal pretreatment and ultrasonic pretreatment, were used to enhance biohydrogen production from sewage sludge. The experimental results showed that the four pretreatments could all increase the soluble chemical oxygen demand (SCOD) of sludge and decrease the dry solid (DS) and volatile solid (VS) because the pretreatments could disrupt the floc structure and even the microbial cells of sludge. The results of batch anaerobic fermentation experiments demonstrated that all of the four pretreatments could select hydrogen-producing microorganisms from the microflora of sludge and enhance the hydrogen production. The hydrogen yield of the alkaline pretreated sludge at initial pH of 11.5 was the maximal (11.68 mL H2/g VS) and that of the thermal pretreated sludge was the next (8.62 mL H2/g VS). The result showed that the hydrogen yield of pretreated sludge was correlative with its SCOD. The hydrogen yields of acid pretreated sludge and alkaline pretreated sludge were also influenced by their initial pH. No methane could be detected in the anaerobic fermentation of alkaline pretreated sludge and thermal pretreated sludge, which suggested that these pretreatments could fully inhibit the activity of methanogens. The volatile fatty acids (VFA) production in anaerobic fermentation of alkaline pre- treated sludge was the maximum and the next is that of thermal pretreated sludge. | XIAO BenYi LIU JunXin | 2009 | Chinese Science Bulletin2009，54，12： | 6 |
| 19 | Recombinant adenovirus-p53(Gendicine) sensitizes a pancreatic carcinoma cell line to radiation显示文摘Objective:In this study,we examine the effects of recombinant adenovirus-p53(rAd-p53)on the pancreatic carcinoma cell line SW1990.Specifically,we determine if expression of rAd-p53 sensitizes these cells to radiation.Methods:Following transfection of SW1990 cells with rAd-p53,we measured expression of P53,P21 and Bax by immunocytochemistry.Both transfected and control cell lines were irradiated with a range of doses,and the survival fractions(SF)were calculated.Dose survival curves were constructed and modeled for comparison.Results:Transfection of SW1990 cells with rAd-p53 resulted in increased expression of P53,P21 and Bax in a time-dependent manner.At 96 h after transfection,89.92%of cells expressed P53,56.8%expressed P21,and 76.50%expressed Bax.The SF following radiation was lower in the rAd-p53 transfected cells compared to the control cells,suggesting that rAd-p53 sensitizes SW1990 cells to radiation(D0for the experimental and control groups was 2.199 and 2.462,respectively).Conclusions:Use of the adenoviral vector is an effective means of transfecting SW1990 cells with wild-type P53,and this sensitizes the cell line to irradiation.This work suggests that combining rAd-p53with radiation therapy in pancreatic cancer may be therapeutically beneficial. | Jinluan Li Jianji Pan Xianggao Zhu Ying Su Lingling Bao Sufang Qiu Changyan Zou Yong Cai Junxin Wu Ivan W.K.Tham | 2013 | Chinese Journal of Cancer Research2013，25，6： | 6 |
| 20 | A study on JA-and BTH-induced resistance of Rosa rugosa‘Plena’to powdery mildew(Sphaerotheca pannosa)显示文摘Different concentrations of jasmonic acid(JA)and benzothiadiazole(BTH) were sprayed on 2-year-old Rosa rugosa‘Plena’ seedlings. The induced resistance of JA and BTH to Sphaerotheca pannosa(Wallr.) and the changes of their related physiological indices were investigated. Results showed that JA and BTH treatments had inhibitory impacts on S. pannosa infection. The optimal concentration of JA and BTH was 0.5 mmol/L for the disease-resistance induction of the leaves, its inductive effect was up to 66.36% for BTH and 54.49% for JA. Our results confirmed that exogenous JA and BTH significantly improved R. rugose ‘Plena’ resistance to S. pannosa. When treated with JA and BTH, activities of the three defense enzymes(POD, PPO, and PAL) increased significantly.Contents of total phenolics, flavonoids, and lignin also increased significantly. It is inferred from these results that exogenous JA and BTH could improve the resistance of R.rugose ‘Plena’ to S. pannosa through enhancing activities of the defensive enzymes and accumulation of secondary metabolites in the leaves. | Junxin Yan Yanan Deng Jia Yu Yongqiang Zhang Defu Chi | 2018 | Journal of Forestry Research2018，29，3： | 6 |
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