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您的检索式：作者名="Jonathan M.Cooper"
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| 1 | Synchronous nanoscale topographic and chemical mapping by differential-confocal controlled Raman microscopy显示文摘Confocal Raman microscopy is currently used for label-free optical sensing and imaging within the biological,engineering,and physical sciences as well as in industry.However,currently these methods have limitations,including their low spatial resolution and poor focus stability,that restrict the breadth of new applications.This paper now introduces differential-confocal controlled Raman microscopy as a technique that fuses differential confocal microscopy and Raman spectroscopy,enabling the point-to-point collection of three-dimensional nanoscale topographic information with the simultaneous reconstruction of corresponding chemical information.The microscope collects the scattered Raman light together with the Rayleigh light,both as Rayleigh scattered and reflected light(these are normally filtered out in conventional confocal Raman systems).Inherent in the design of the instrument is a significant improvement in the axial focusing resolution of topographical features in the image(to^1 nm),which,when coupled with super-resolution image restoration,gives a lateral resolution of 220 nm.By using differential confocal imaging for controlling the Raman imaging,the system presents a significant enhancement of the focusing and measurement accuracy,precision,and stability(with an antidrift capability),mitigating against both thermal and vibrational artefacts.We also demonstrate an improved scan speed,arising as a consequence of the nonaxial scanning mode. | HAN CUI YUN WANG LIRONG QIU SHUCHENG LI JONATHAN M.COOPER AND WEIQIAN ZHAO | 2020 | Photonics Research2020，8，9： | 1 |
| 2 | Branched hybridization chain reaction-using highly dimensional DNA nanostructures for label-free,reagent-less,multiplexed molecular diagnostics显示文摘The specific and multiplexed detection of DNA underpins many analytical methods,including the detection of microorganisms that are important in the medical,veterinary,and environmental sciences.To achieve such measurements generally requires enzyme-mediated amplification of the low concentrations of the target nucleic acid sequences present,together with the precise control of temperature,as well as the use of enzyme-compatible reagents.This inevitably leads to compromises between analytical performance and the complexity of the assay.The hybridization chain reaction(HCR)provides an attractive alternative,as a route to enzyme-free DNA amplification.To date,the linear nucleic acid products,produced during amplification,have not enabled the development of efficient multiplexing strategies,nor the use of label-free analysis.Here,we show that by designing new DNA nanoconstructs,we are able,for the first time,to increase the molecular dimensionality of HCR products,creating highly branched amplification products,which can be readily detected on label-free sensors.To show that this new,branching HCR system offers a route for enzyme-free,label-free DNA detection,we demonstrate the multiplexed detection of a target sequence(as the initiator)in whole blood.In the future,this technology will enable rapid point-of-care multiplexed clinical analysis or in-the-field environmental monitoring. | Gaolian Xu Mingliang Lai Rab Wilson Andrew Glidle Julien Reboud Jonathan M.Cooper | 2019 | Microsystems & Nanoengineering2019，5，1： | 0 |
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