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| 1 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 2 | A Strategy for Searching Antigenic Regions in the SARS-CoV Spike Protein显示文摘In the face of the worldwide threat of severe acute respiratory syndrome (SARS)to human life, some of the most urgent challenges are to develop fast and accurate analytical methods for early diagnosis of this disease as well as to create a safe anti-viral vaccine for prevention. To these ends, we investigated the antigenicity of the spike protein (S protein), a major structural protein in the SARS-coronavirus (SARS-CoV). Based upon the theoretical analysis for hydrophobicity of the S protein, 18 peptides were synthesized. Using Enzyme-Linked Immunosorbent Assay (ELISA), these peptides were screened in the sera from SARS patients. According to these results, two fragments of the S gene were amplified by PCR and cloned into pET-32a. Both S fragments were expressed in the BL-21 strain and further purified with an affinity chromatography. These recombinant S fragments were confirmed to have positive cross-reactions with SARS sera, either by Western blot or by ELISA. Our results demonstrated that the potential epitope regions were located at Codons 469-882 in the S protein, and one epitope site was located at Codons 599-620. Identification of antigenic regions in the SARS-CoV S protein may be important for the functional studies of this virus or the development of clinical diagnosis. | Yan Ren, Zhengfeng Zhou, Jinxiu Liu, Liang Lin, Shuting Li, Hao Wang, Ji Xia, Zhe Zhao, Jie Wen, Cuiqi Zhou, Jingqiang Wang, Jianning Yin, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China. | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 1 |
| 3 | Estimating EC and ionic EC contribution percentage of nutrient solution based on ionic activity显示文摘The dynamic monitoring technology of inorganic ions using ion selective electrodes has some problems such as low precision,vulnerability to other ions,short service life,and high price.Due to the difficulty of dynamic control based on ionic concentration of nutrient solution,EC and pH values of nutrient solution are often used as feedback control indexes in hydroponic system.In this study,estimation algorisms of EC and ionic EC contribution percentage based on ionic activity were proposed to understand the quantitative relationship between ionic concentration and EC.With a view to predicting the EC accurately by mean ionic activities of specific salts in nutrient solution based on a specific formula,ionic concentration could also be calculated by the actual measurement of EC combined with ionic EC contribution percentage.With Japanese horticultural experimental nutrient formula and Yamasaki tomato nutrient formula,significant linear correlations between estimated EC and measured EC were found with determination coefficients over 0.99.Ionic EC contribution percentage was not affected by different relative concentrations of nutrient solutions.However,ionic EC contribution percentage changed significantly when adding specific salts with different concentrations,and different changes were found in each anions and cations of specific salt added.When the same K+concentration was added in different forms of KNO_(3),K_(2_SO_(4),KCl,and KH_(2)PO_(4),the changes of ionic EC contribution percentage of K^(+)were similar,but those of other anions in potassium salts varied greatly.The relative errors of estimated EC of nutrient solutions based on ionic activities were only 1.3%in horticultural experimental nutrient solution and 1.8%in Yamasaki tomato nutrient solution with different relative concentrations compared to measured EC.The relative errors of estimated EC of nutrient solutions with specific salt added were only 0.1%-0.5%compared to measured EC in two nutrient solution.Therefore,the dynamic feedback control of ionic concentration of nutrient solution could be realized by using EC measurement combined with ionic EC contribution percentage to improve the ionic quantitative control in nutrient solution.The EC control of nutrient solution in automatic irrigation system might be upgraded to ionic concentration control by using algorisms above of ionic EC contribution percentage and EC estimation to meet dynamic demands of hydroponic crops for ionic concentration in different growth stages. | Jinxiu Song Lin Xu Dongxian He Satoru Tuskagoshi Toyoki Kozai Yutaka Shinohara | 2019 | International Journal of Agricultural and Biological Engineering2019，12，2： | 1 |
| 4 | DESIGN CONCEPT FOR SNAP-ACTING TWO-WAY SHAPE MEMORY DEVICES显示文摘Snap-acting two way shape memory device can be obtained by using the design proposed byauthors.Some essential parameters which characterize the properties of device have been pro-posed also in this paper.And the principle and method according to which a practicalsnap-acting device can be designed have been described. | LIN Guangming CHENXingyuan ZHANG Jinxiu Material Science Research Institute,Zhongshan University,Guangzhou,China WANG Yurui Metallurgy Research Institute of Beijing Iron and Steel Company,Beijing,China LIN Guangming Associate Professor,Department of Physics,Zhongshan University,Guangzhou 510275,China | 1990 | Acta Metallurgica Sinica(English Letters)1990，3，2： | 0 |
| 5 | Research on Dynamic Discovery Model of User Interest Based on Time and Space Vector显示文摘 | Jinxiu Lin Zhaoxin Zhang Lejun Chi Yang Wang | 2018 | 国际计算机前沿大会会议论文集2018，，2： | 0 |
| 6 | Tailoring impedance match and enhancing microwave absorption of Fe_3O_4/Bi_(24)Fe_2O_(39)/Bi hollow porous microrods by controlling their composition显示文摘A self-assembly/precipitate conversion/decomposition process was developed for the controllable synthesis of Fe_3O_4/Bi_(24)Fe_2O_(39)/Bi hollow porous microrods(HPMRs).The results demonstrated that the crystal size,component,and performances of HPMRs could be effectively modulated via changing Fe^(2+)/Bi^(3+)molar ratio(γ).Fe_3O_4/Bi_(24)Fe_2O_(39)/Bi HPMRs exhibited ferromagnetic behavior at room temperature.As Bi and Bi_(24)Fe_2O_(39)contents increased withγ,the saturation magnetization M_sand attenuation constantly decreased,whereas coercivity H_cand impedance matching ratio increased.Compounding Fe_3O_4with small quantities of Bi and Bi_(24)Fe_2O_(39)into HPMRs can significantly enhance microwave absorption.Fe_3O_4/Bi_(24)Fe_2O_(39)/Bi HPMRs formed atγ=1:0.25 exhibited the optimum microwave absorption performance.The minimum R_Lwas-47.3 dB at8.72 GHz,corresponding to 2.4 mm sample thickness.The absorption band with the reflection loss below-20 dB was up to 14.0 GHz for the absorber with a thickness of 1.4-8.0 mm.The results demonstrate that the introduction of electromagnetic transparent materials(Bi_(24)Fe_2O_(39)or Bi)can improve the microwave absorption performances of Fe_3O_4 composites owing to enhanced impedance matching rather than attenuation constant. | Lin Liu Na He Jiacheng Sun Panbing Hu Rujia He Jinxiu Cheng Weifu Tian Guoxiu Tong | 2018 | Progress in Natural Science:Materials International2018，28，5： | 0 |
| 7 | SNAP-ACTION PHASE TRANSFORMATION IN TWO-WAY SHAPE MEMORY Ni-Ti ALLOY显示文摘The snap-action behavior of a Ni-Ti alloy disc which is controlled by combination of anonlinear stress field and temperature has been studied.After treatment for two-way shapememory,all shape memory strain of snap-action finishes abruptly at a certain temperaturewithin an interval of less than 1 ms.The results of resistance measurement and in-situ X-raydiffraction indicate that the snap-action strain is mainly resulted from the snap-action β R transformation. | LUO Laizhong ZHANG Jinxiu LIN Guangming Zhongshan University,Guangzhou,510275,China Lecturer,Dept.of Physics and Institute of Material Science,Zhongshan University,Guangzhou,510275,China | 1989 | Acta Metallurgica Sinica(English Letters)1989，2，5： | 0 |
| 8 | Study of Snap-acting Two Way Shape Memory Effect in NiTi Alloy显示文摘Snap-acting transformation and snap-acting two way shape memory(TWSM)effect have been obtained in NiTi alloy usingthe constrained effect of stress on the driving force of TWSM element.The TWSM strain of the snap-acting element made ofNi51Ti49 alloy finishes abruptly at an acertain temperature without any additional mechanism,within an interval less than 1 ms.Athermodynamic model is presented to account for these observations. | Lin, Guangming Zhang, Jinxiu Lou, Laizhong | 1989 | Rare Metals1989，9，3： | 0 |
| 9 | Research on community detection algorithm based on site topic similarity and topology显示文摘Considering the deviation in content of community detection resulting from the low accuracy of resource relevance,an algorithm based on the topology of sites and the similarity between their topics is proposed. With topic content factors fully considered,this algorithm can search for topically similar site clusters on the premise of inter-site topology. The experimental results show that the algorithm can generate a more accurate result of detection in the real network. | 胡艺 Li Zhengmin Chi Lejun Lin Jinxiu | 2018 | High Technology Letters2018，24，2： | 0 |
| 10 | Image Denoising with Adaptive Weighted Graph Filtering显示文摘Graph filtering,which is founded on the theory of graph signal processing,is proved as a useful tool for image denoising.Most graph filtering methods focus on learning an ideal lowpass filter to remove noise,where clean images are restored from noisy ones by retaining the image components in low graph frequency bands.However,this lowpass filter has limited ability to separate the low-frequency noise from clean images such that it makes the denoising procedure less effective.To address this issue,we propose an adaptive weighted graph filtering(AWGF)method to replace the design of traditional ideal lowpass filter.In detail,we reassess the existing low-rank denoising method with adaptive regularizer learning(ARLLR)from the view of graph filtering.A shrinkage approach subsequently is presented on the graph frequency domain,where the components of noisy image are adaptively decreased in each band by calculating their component significances.As a result,it makes the proposed graph filtering more explainable and suitable for denoising.Meanwhile,we demonstrate a graph filter under the constraint of subspace representation is employed in the ARLLR method.Therefore,ARLLR can be treated as a special form of graph filtering.It not only enriches the theory of graph filtering,but also builds a bridge from the low-rank methods to the graph filtering methods.In the experiments,we perform the AWGF method with a graph filter generated by the classical graph Laplacian matrix.The results show our method can achieve a comparable denoising performance with several state-of-the-art denoising methods. | Ying Chen Yibin Tang Lin Zhou Yan Zhou Jinxiu Zhu Li Zhao | 2020 | Computers, Materials & Continua2020，，8： | 0 |
| 11 | TRANSFORMATION CHARACTERISTIC OF TWO WAY SHAPE MEMORY MATERIAL CONSTRAINED BY STRESS显示文摘The snap-acting transition and two way shape memory (TWSM) effect have been obtainedin a TWSM spring made of Ni51Ti49 alloy using the constrained effect of stress on drivingforce of TWSM material.When the snap-acting transition has been realized:M_s= M_f andA_s=A_f;hysteresis is rectangular,the height (TWSM effect) and width (hysteresis) of therectangle can be readjusted by means of constrained stress.The results of resistancemeasurments show that,the snap-acting TWSM effect mentioned above was supplied mainlyfrom the transformation of P←→R.The thermodynamic model has also been proposed to ac-count for the snap-acting transformation. | LIN Guangming HUANG Yuanshi ZHANG Jinxiu Zhongshan University,Guangzhou,China LIN Guangming,Associate Professor,Dept.of Physics,Zhongshan University,Guangzhou 510275,China | 1990 | Acta Metallurgica Sinica(English Letters)1990，3，3： | 0 |
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