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| 1 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 2 | The synthesis, bioactivity and paramagnetic resonance of angiotensin Ⅱ and its spin labelled derivative显示文摘ANGIOTENSIN Ⅱ(Ang Ⅱ) is an important constituent in renin-angiotension system (RAS).The amino acid sequence of Ang Ⅱ is DRVYIHPF. Ang Ⅱ plays an important role both inmaintenance of normal blood pressure and in occurrence of hypertension. Ang Ⅱ binding re-ceptor can induce many kinds of physiological effects. Spin labeling is an effective method thatgreatly deepened the knowledge in structure, movement and interaction of biologicalmolecules. For example, it has been used in studying interaction of antigen and antibody suc- | WANG Rui, LI Xiaoxu, HU Xiaoyu, NI Jingman, LI Changling and LU Jingfen1. Department of Biology, State Key Laboratory of Applied Organic Chemistry, Lanzhou 730000, China 2. Department of Chemistry, Lanzhou University, Lanzhou 730000 3. State Key Laboratory of Natural and Biomimetic Drugs, Beijing Medical U-niversity, Beijing 100083, China. | 1997 | Chinese Science Bulletin1997，42，21： | 2 |
| 3 | synthesis and stability avaluation of 5 - in - actic Podophyllic ester as anti-turner agent 显示文摘 | Rong Xing Zhang Fumin Jingman N | 2007 | Chimese Pharmaceilical Sciences2007，，16： | 1 |
| 4 | Association of an Increased Risk of Pre-eclampsia and Fetal Growth Restriction in Singleton and Twin Pregnancies with Female Fetuses显示文摘Objective: To investigate whether the fetal gender affects the incidence of pre-eclampsia (PE) and fetal growth restriction (FGR) in singleton and twin pregnancies.Methods: This was a 10-year single-center, retrospective, cohort study from January 2009 to January 2019. A total of 57,129 singleton and 3699 twin pregnancies aged between 18-55 years old were recruited at the Third Affiliated Hospital of Guangzhou Medical University, China. We used multivariable logistic regression to analyze the effect of fetal gender on the incidence of PE and FGR.Results: In singleton pregnancies, the incidence rates of PE and FGR with a female fetus were higher than those with a male fetus (6.4% (1713/26,793)vs. 5.9% (1803/30,336),P < 0.05 and 3.5% (932/26,793)vs. 2.4% (745/30,336),P < 0.05, respectively). A female fetus was an independent risk factor for either PE or FGR (adjusted odds ratio: 1.169 or 1.563;95% confidence interval: 1.036-1.319 or 1.349-1.810, respectively). In twin pregnancies, the incidence of early-onset PE was greater in pregnancies with two females compared with two males or one male plus one female (4.6% (46/1003)vs. 4.1% (54/1305)vs. 2.4% (33/1391),P < 0.05). Female-female twins was an independent risk factor for PE (adjusted odds ratio: 1.367, 95% confidence interval: 1.011-1.849), especially early-onset PE.Conclusion: The female fetus was associated with PE in both singleton and twin pregnancies and was also a risk factor of FGR in singleton pregnancies. | Shilei Bi Lizi Zhang Zhijian Wang Jingman Tang Sushan Xie Jingjin Gong Lin Lin Luwen Ren Lijun Huang Shanshan Zeng Jingsi Chen Lili Du Dunjin Chen | 2021 | Maternal-Fetal Medicine2021，3，1： | 1 |
| 5 | Effect of total flavoues of buckwheat flowers and leaves on protein tyrosine phosphatase 1B expression in type 2 diabetic rats显示文摘 | GOU Xiangbo HAN Shuying XU Jingman | | 0，，07： | 1 |
| 6 | The improved blood–brain barrier permeability of endomorphin-1 using the cell-penetrating peptide synB3 with three different linkages显示文摘 | Hui Liu Wei Zhang Linnan Ma Linlan Fan Feiyun Gao Jingman Ni Rui Wang | 2014 | International Journal of Pharmaceutics . 2014 (1-2)2014，，： | 1 |
| 7 | iCatch:a new strategy for capturing large DNA fragments using homing endonucleases显示文摘Natural genetic materials contain many biosynthetic gene clusters encoding potentially valuable natural products,many of which can be used directly without codon optimization or other manipulations.With the development of synthetic biology,several DNA assembly standards have been proposed,conveniently facilitating the reuse of natural materials.Among these standards,the iBrick assembly standard was developed by our laboratory to manipulate large DNA fragments,employing two homing endonucleases.Considering the difficulty of cloning large iBrick parts using conventional endonuclease-mediated restriction and ligation methods,we herein present a new method,known as iCatch,which readily captures biosynthetic gene clusters.As the clusters cloned by iCatch have the prefix and suffix of the iBrick standard,they serve as new iBrick parts and are therefore conducive to further editing and assembly with the iBrick standard.iCatch employs the natural homologous recombination system to flank the region of interest with I-Scel and PI-Pspl recognition sites,after which the genome is digested with I-Scel or PI-Pspl and the fragments are then self-ligated to clone the target DNA fragments.We used this method to successfully capture the actinorhodin biosynthetic cluster from Streptomyces coelicolor and then heterologously expressed this cluster in a thermophilic Streptomyces strain.We propose that iCatch can be used for the cloning of DNA sequences that are dozens of kilobases in length,facilitating the heterologous expression of microbial natural products.Moreover,this cloning methodology can be a complementary tool for the iBrick standard,especially in applications requiring the manipulation of large DNA fragments. | Jingman Wang Anrui Lu Jiakun Liu Weiren Huang Jin Wang Zhiming Cai Guoping Zhao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，1： | 0 |
| 8 | Design, Synthesis, Anticancer Pharmacology of Actinomycin D Analogues: 5,5' -MeSer2-ActD and 5,5' -MeAla2-ActD显示文摘Introduction
Actinomycin D (ActD, Figure 1.), containing a planar phenoxazone ring and two cyclic pentapeptides, is one of the most intensely studied anticancer drugs and currently used to treat highly malignant tumors, such as Wilms' tumor and gestational choriocarcinoma. Although ActD possesses high antitumor activities, its clinical usefulness is limited by its extreme cytotoxicity. Thus, if the structure of ActD can be modified to reduce its cytotoxicity while retaining its activity, such an analogue would be a better antitumor drug. On the basis of X-ray crystal structures of the complexes between ActD and DNA[1], and our work [2], we designed and totally synthesized two ActD analogs 8a-b iii which both of the (L)-N-methyl-valine residues of ActD were replaced with LN-MeAla and L-N-MeSer, respectively. The anticancer pharmacology of the two analogs were examined. The result shows that the toxicity of the two analogs are higher than ActD and the antitumoe activity are lower than ActD, too. | NI JingMan | 2001 | Chemical Research in Chinese Universities2001，17，z1： | 0 |
| 9 | Grade and Tonnage Model of Contact Metasomatic Copper Deposit in China显示文摘Grade-tonnage model is one of the research frontiers of systematical exploration theory. Based on the “Reserve Database of Mineral Resources in China (1997)”, this paper establishes the geological model, grade model, tonnage model, grade-tonnage model and tonnage-sequence model of contact metasomatic copper deposits in China. The mathematical properties of these models are described in detail. | Wang Xueping Wei Min Faculty of Earth Resources, China University of Geosciences, Wuhan 430074 Yang Lipei Zhao Jingman China Mineral Information Research Institute, Beijing 100037 | 2000 | Journal of Earth Science2000，19，1： | 0 |
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