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1Stimulation of melanogenesis by scoparone in B16 melanoma cells显示文摘Aim:The effect of coumarin derivatives on melanogenesis was investigated inB16 murine melanoma cells.Methods:Melanin content and tyrosinase activitywere analyzed spectrophotometrically.The expression of tyrosinase,tyrosinase-related protein-1(TRP-1)and tyrosinase-related protein-2(TRP-2)were measuredeither by reverse transcription-polymerase chain reaction(RT-PCR)or Westernblot.Results:Among the coumarin derivatives studied,scoparone(6,7-dimethoxycoumarin)was the most potent;the 6-or 7-methoxy group was found tobe essential for the stimulation of melanogenesis.The melanin content was greatlyincreased by scoparone in a dose-dependent manner;there was no cytotoxicity atthe effective concentrations.Scoparone increased enzyme activity as well asprotein and mRNA expression of tyrosinase.In addition,mRNA of TRP-1 andTRP-2 were also increased after treatment with scoparone.H-89,an inhibitor ofprotein kinase A(PKA),completely inhibited the scoparone-induced increase ofmelanogenesis and the tyrosinase protein.Conclusion:These results suggestthat scoparone-induced stimulation of melanogenesis is likely to occur at thetranscriptional level of melanogenesis-related enzymes through PKA signaling.Jeong-yeh YANG Jeung-hyunKOO Young-gilSONG Kang-beom KWON Ju-hyung LEE Hee-sook SOHN Byung-hyun PARK Eun-chung JHEE Jin-woo PARK 2006Acta Pharmacologica Sinica2006,27,11:2
2Cystathionine beta-synthase deficiency in Georgia(USA) ; correlation of clinical and biochemical phenotype with genotype 显示文摘Kruger W D Wang L Jhee Kee 2003Hum Mutat2003,22,6:1
3Production of the neuromodulator H2 S by cystathionine beta-synthase via the condensation of cysteine and homocysteine 显示文摘Chert X Jhee KH Kruger WD 2004J Biol Chem2004,279,52:1
4Effect of surface treatments on the frosting defrostingbehavior of a fin-tube heat ex- changer 显示文摘Jhee S Lee KS Kim WS 2002International Journal of Refrigeration2002,25,8:1
5Direct determinationof verapamil in rat plasma by coupled column microbore-HPLC method 显示文摘JHEE O H HONG J W OM A S 2005J Pharm Biomed Anal2005,37,2:1
6Generative CAPP through projective feature recognition显示文摘LEE H C JHEE W C PARK H S 2007Computers and Industrial Engineering2007,53,2:1
7Effect of surface treatments on the frosting/defrosting behavior of a fin-tube heat exchanger 显示文摘Jhee Sung Lee Kwan-Soo Kim Woo-Seung 2002International Journal of Refrigeration2002,25,8:1
8Public Support for Regional Integration in Northeast Asia: An Empirical Test of Affective and Utilitarian Models显示文摘Byong - kuen JHEE 2009International Political Science Review2009,,1:1
9Production of the neuromodulator H2S by cystathionine β-Synthase via the condensation of cysteine and homocysteine显示文摘Chen XL Jhee KH Kruger WD 2004J Biol Chem2004,279,52:1
10Prediction of the frost formation on a cold flat surface显示文摘Lee KS Jhee S Yang DK 2003Int J Heat Mass Transfer2003,46,20:1
11Prediction of the frost formation on a cold flat surface显示文摘Lee K S Jhee S Yang D K 2003Int J Heat Mass Transfer2003,46,20:1
12A case-based expert system approach for quality design 显示文摘SUH M S JHEE W C KO Y K 1998Expert Systems With Applications1998,,15:1
13Effect of surface treatments on the frosting/defrosting behavior of a fin-tube heat exchanger显示文摘Sung Jhee Kwan-Soo Lee Woo-Seung Kim 2002International Journal of Refrigeration2002,,8:1
14Pharmacokinetic and bioequivalence evaluation of two formulations of 100 mg trimebutine maleate(Recutin?and Polybutin?)in healthy male volunteers using the LC-MS/MS method显示文摘Jhee OH Lee YS Shaw LM 2007Clinica Chimica Acta2007,375,1:1
15Modulation of cystathionine{beta } -syntase level regulates total serum homocysteine in mice显示文摘Wang L Jhee KH Hua X 2004Circ Res2004,94,10:1
16Comparison of aminoglycoside pharmacokinetics in Asian, Hispanic, and Caucasian patients by using population pharmacokinetic methods 显示文摘Jhee SS Burm JP Gill MA 1994Antimicrob Agents Chemother1994,38,:1
17The role of cystathionine beta-synthase in homocysteine metabolism显示文摘JHEE K H KRUGER W D 2005Antioxid Redox Signal2005,7,56:1
18The role of cystathionine beta-synthase in homocysteine metabolism显示文摘Jhee KH Kruger WD 2005Antioxid Redox Signal2005,7,56:1
19Effect of surface treatments on the frosting/defrosting behavior of a fin-tube heat exchanger 显示文摘JHEE S LEE K S Kim W S 2002Int J Refrig2002,25,:1
20Line width characteristics of fiber extended cavity distributed-feedback lasers 显示文摘Liou K Y Jhee Y K Eisenstein G 1986APL1986,48,16:1
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