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| 1 | Toward point-of-care management of chronic respiratory conditions:Electrochemical sensing of nitrite content in exhaled breath condensate using reduced graphene oxide显示文摘We present a portable non-invasive approach for measuring indicators of inflammation and oxidative stress in the respiratory tract by quantifying a biomarker in exhaled breath condensate(EBC).We discuss the fabrication and characterization of a miniaturized electrochemical sensor for detecting nitrite content in EBC using reduced graphene oxide.The nitrite content in EBC has been demonstrated to be a promising biomarker of inflammation in the respiratory tract,particularly in asthma.We utilized the unique properties of reduced graphene oxide(rGO);specifically,the material is resilient to corrosion while exhibiting rapid electron transfer with electrolytes,thus allowing for highly sensitive electrochemical detection with minimal fouling.Our rGO sensor was housed in an electrochemical cell fabricated from polydimethyl siloxane(PDMS),which was necessary to analyze small EBC sample volumes.The sensor is capable of detecting nitrite at a low over-potential of 0.7 V with respect to an Ag/AgCl reference electrode.We characterized the performance of the sensors using standard nitrite/buffer solutions,nitrite spiked into EBC,and clinical EBC samples.The sensor demonstrated a sensitivity of 0.21μAμM^(−1) cm^(−2) in the range of 20–100μM and of 0.1μAμM^(−1) cm^(−2) in the range of 100–1000μM nitrite concentration and exhibited a low detection limit of 830 nM in the EBC matrix.To benchmark our platform,we tested our sensors using seven pre-characterized clinical EBC samples with concentrations ranging between 0.14 and 6.5μM.This enzyme-free and label-free method of detecting biomarkers in EBC can pave the way for the development of portable breath analyzers for diagnosing and managing changes in respiratory inflammation and disease. | Azam Gholizadeh Damien Voiry Clifford Weisel Andrew Gow Robert Laumbach Howard Kipen Manish Chhowalla Mehdi Javanmard | 2017 | Microsystems & Nanoengineering2017，3，1： | 5 |
| 2 | Toward point-of-care assessment of patient response:a portable tool for rapidly assessing cancer drug efficacy using multifrequency impedance cytometry and supervised machine learning显示文摘We present a novel method to rapidly assess drug efficacy in targeted cancer therapy,where antineoplastic agents are conjugated to antibodies targeting surface markers on tumor cells.We have fabricated and characterized a device capable of rapidly assessing tumor cell sensitivity to drugs using multifrequency impedance spectroscopy in combination with supervised machine learning for enhanced classification accuracy.Currently commercially available devices for the automated analysis of cell viability are based on staining,which fundamentally limits the subsequent characterization of these cells as well as downstream molecular analysis.Our approach requires as little as 20μL of volume and avoids staining allowing for further downstream molecular analysis.To the best of our knowledge,this manuscript presents the first comprehensive attempt to using high-dimensional data and supervised machine learning,particularly phase change spectra obtained from multi-frequency impedance cytometry as features for the support vector machine classifier,to assess viability of cells without staining or labelling. | Karan Ahuja Gulam M.Rather Zhongtian Lin Jianye Sui Pengfei Xie Tuan Le Joseph R.Bertino Mehdi Javanmard | 2019 | Microsystems & Nanoengineering2019，5，1： | 4 |
| 3 | Fully integrated wearable impedance cytometry platform on flexible circuit board with online smartphone readout显示文摘We present a wearable microfluidic impedance cytometer implemented on a flexible circuit wristband with on-line smartphone readout for portable biomarker counting and analysis.The platform contains a standard polydimethylsiloxane(PDMS)microfluidic channel integrated on a wristband,and the circuitry on the wristband is composed of a custom analog lock-in amplification system,a microcontroller with an 8-bit analog-to-digital converter(ADC),and a Bluetooth module wirelessly paired with a smartphone.The lock-in amplification(LIA)system is implemented with a novel architecture which consists of the lock-in amplifier followed by a high-pass filter stage with DC offset subtraction,and a post-subtraction high gain stage enabling detection of particles as small as 2.8μm using the 8-bit ADC.The Android smartphone application was used to initiate the system and for offline data-plotting and peak counting,and supports online data readout,analysis,and file management.The data is exportable to researchers and medical professionals for in-depth analysis and remote health monitoring.The system,including the microfluidic sensor,microcontroller,and Bluetooth module all fit on the wristband with a footprint of less than 80 cm2.We demonstrate the ability of the system to obtain generalized blood cell counts;however the system can be applied to a wide variety of biomarkers by interchanging the standard microfluidic channel with microfluidic channels designed for biomarker isolation. | Abbas Furniturewalla Matthew Chan Jianye Sui Karan Ahuja Mehdi Javanmard | 2018 | Microsystems & Nanoengineering2018，4，1： | 2 |
| 4 | Circulating endo- thelial cells (eees) and e-selectfn: predictors of preeclampsia显示文摘 | Mehrabian F Jazi SM Javanmard SH | 2012 | J Res Med Sei2012，17，1： | 1 |
| 5 | Diabetes mellitus and tuberculosis facts and controversies 显示文摘 | Baghaei P Marjani M Javanmard P | 2013 | J Diabetes Metab Disord2013，12，1： | 1 |
| 6 | Is it necessary to place a double j catheter after laparoscopie ureterolithotomy? A four-year experience 显示文摘 | Karami H Javanmard B Hasanzadeh-Hadah A | 2012 | J Endourol2012，26，9： | 1 |
| 7 | Electrical detection of protein bimarkers using bioactivated microfluidic channels 显示文摘 | Javanmard M Talasaz A H Nemat-Gorgani M | 2009 | Lab Chip2009，9，： | 1 |
| 8 | The effects of blocking Angiotensin receptors on early stages of diabetic nephropathy显示文摘 | GHEISSARI A JAVANMARD SH SHIRZADI R | 2012 | Int J Prey Med2012，3，7： | 1 |
| 9 | Targeted cell detection based on microchannel gating 显示文摘 | Javanmard M Talasaz A H | 2007 | Biomicrofluidics2007，1，4： | 1 |
| 10 | Lep- tin promotes melanoma tumor growth in mice related to in- creasing circulating endothelial progenitor cells numbers and plasma NO production 显示文摘 | Amjadi F Javanmard SH Zarkesh- Esfahani H | 2011 | Journal of Experimental & Clini- cal Cancer Research2011，30，1： | 1 |
| 11 | A review of functional neuroimaging in mood disorders: positron emission tomography and depression显示文摘 | Kennedy SH Javanmard M Vaecarino FJ | 1997 | Can J Psychiatry1997，42，： | 1 |
| 12 | Adiponectin gene polymorphisms and susceptibility to atherosclerosis: a meta- analysis显示文摘 | Mansourian M Javanmard SH | 2013 | J Med Sci2013，18，7： | 1 |
| 13 | Aqueous ~oncentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients显示文摘 | Javanmard SH Hasanpour ZH Abbaspoor Z | 2012 | J Res Med Sci2012，17，12： | 1 |
| 14 | Hyperinsulinemia may have a protective role in the early stages of atherosclerosis in rabbit model of hypercholesterolemia显示文摘 | Javanmard SH Nematbakhsh M Feghhi A | 2012 | J Diabetes Metab Disord2012，11，1： | 1 |
| 15 | Effects of gamma irradiation and frozen storage on microbial, chemical and sensory quality of chicken meat in Iran 显示文摘 | Javanmard M Rokni N | 2006 | Food Control2006，17，： | 1 |
| 16 | Is it necessary to place double J catheter after laparoscopic ureterolithotomy? A four-year experience显示文摘 | Karami H Javanmard B Hasanzadeh-Hadah A | 2012 | J Endourol2012，26，9： | 1 |
| 17 | Mutations in bone morphogenetic proteinl5 and growth differentiation factor9 genes are associated with increased litter size in fat - tailed sheep breeds 显示文摘 | ARASH JAVANMARD AZADZADEH N ESMAILIZADEH K | 2011 | Vet Res Commun2011，35，： | 1 |
| 18 | Effect of L-arginine on circulating endothelial progenitor cells in hypercholesterolemic rabbits显示文摘 | Javanmard S H Gheisari Y Soleimani M | 2010 | International Journal of Cardiology2010，，6： | 1 |
| 19 | Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds显示文摘 | JAVANMARD A AZADZADEH N ESMAILIZADEH A K | 2011 | Vet Res Commun2011，35，3： | 1 |
| 20 | Apolipoprotein A1 as a Novel anti-implantation biomarker in polycystic ovary syndrome :A case-control study显示文摘 | Amjadi F Aflatoonian R Javanmard SH Saifi B Ashrafi M Mehdizadeh M | 2015 | J Res Med Sci2015，20，： | 1 |
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