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您的检索式：作者名="Jake Engel"
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| 1 | Responses of an old-field plant community to interacting factors of elevated[CO_(2)],warming,and soil moisture显示文摘Aims The direct effects of atmospheric and climatic change factors—atmospheric[CO_(2)],air temperature and changes in precipitation—can shape plant community composition and alter ecosystem function.It is essential to understand how these factors interact to make better predictions about how ecosystems may respond to change.We investigated the direct and interactive effects of[CO_(2)],warming and altered soil moisture in open-top chambers(OTCs)enclosing a constructed old-field community to test how these factors shape plant communities.Materials and methods The experimental facility in Oak Ridge,TN,USA,made use of 4-m diameter OTCs and rain shelters to manipulate[CO_(2)](ambient,ambient+300 ppm),air temperature(ambient,ambient+3.5C)and soil moisture(wet,dry).The plant communities within the chambers comprised seven common old-field species,including grasses,forbs and legumes.We tracked foliar cover for each species and calculated community richness,evenness and diversity from 2003 to 2005.Important findings This work resulted in three main findings:(1)warming had speciesspecific effects on foliar cover that varied through time and were altered by soil moisture treatments;(2)[CO_(2)]had little effect on individual species or the community;(3)diversity,evenness and richness were influenced most by soil moisture,primarily reflecting the response of one dominant species.We conclude that individualistic species responses to atmospheric and climatic change can alter community composition and that plant populations and communities should be considered as part of analyses of terrestrial ecosystem response to climate change.However,prediction of plant community responses may be difficult given interactions between factors and changes in response through time. | E.Cayenne Engel Jake F.Weltzin Richard J.Norby Aimee T.Classen | 2009 | Journal of Plant Ecology2009，2，1： | 8 |
| 2 | Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression显示文摘Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway engineering.Along the way,combinatorial gene expression control through modification of regulatory element offered tremendous opportunity for fine-tuning gene expression and generating digital-like genetic circuits.In this report,we present an efficient evolutionary approach to build a range of regulatory control elements.The reported method allows for rapid construction of promoter,5'UTR,terminator and trans-activating RNA libraries.Synthetic overlapping oligos with high portion of degenerate nucleotides flanking the regulatory element could be efficiently assembled to a vector expressing fluorescence reporter.This approach combines high mutation rate of the synthetic DNA with the high assembly efficiency of Gibson Mix.Our constructed library demonstrates broad range of transcriptional or translational gene expression dynamics.Specifically,both the promoter library and 50UTR library exhibits gene expression dynamics spanning across three order of magnitude.The terminator library and trans-activating RNA library displays relatively narrowed gene expression pattern.The reported study provides a versatile toolbox for rapidly constructing a large family of prokaryotic regulatory elements.These libraries also facilitate the implementation of combinatorial pathway engineering principles and the engineering of more efficient microbial cell factory for various biomanufacturing applications. | Erqing Jin Lynn Wong Yun Jiao Jake Engel Benjamin Holdridge Peng Xu | 2017 | Synthetic and Systems Biotechnology2017，2，4： | 2 |
| 3 | Corrigendum to“Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression”[Synth Syst Biotechnol 2(4)(2017)295–301]显示文摘The corresponding author apologizes for listing Lynn Wong and Yun Jiao as co-authors of this manuscript.Triplicate experimental data,including fluorescence,cell density(OD)for the T7 promoter,T7 terminator,ribosome binding sites(RBS)and trans-activating RNA libraries have been uploaded to Mendeley with the DOI number(http://gffzzd3cc09b8251d45dfscnwuoxwc6wkw6k0x.ffgz.tsg.suse.edu.cn/10.17632/skmnwht6dk.1). | Erqing Jin Lynn Wong Yun Jiao Jake Engel Benjamin Holdridge Peng Xu | 2019 | Synthetic and Systems Biotechnology2019，4，4： | 0 |
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