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| 1 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 2 | A literature review of cost-effectiveness of intravenous recombinant tissue plasminogen activator for treating acute ischaemic stroke显示文摘Background Intravenous recombinant tissue plasminogen activator(IV rtPA)is recommended treatment for patients with acute ischaemic stroke,but the cost-effectiveness of IV rtPA within different time windows after the onset of acute ischaemic stroke is not well reviewed.Aims To conduct a literature review of the cost-effectiveness studies about IV rtPA by treatment times.Summary of review A literature search was conducted using MEDLINE,EMBASE,CINAHL and Cochrane Library,with the keywords acute ischemic stroke,tissue plasminogen activator,cost,economic benefit,saving and incremental cost-effectiveness analysis.The review is limited to original research articles published during 1995-2016 in English-language peer-reviewed journals.We found 16 studies meeting our criteria for this review.Nine of them were cost-effectiveness studies of IV rtPA treatment within 0-3 hours after stroke onset,2 studies within 3-4.5 hours,3 studies within 0-4.5 hours and 2 studies within 0-6 hours.IV rtPA is a cost-saving or a cost-effectiveness strategy from most of the study results.Only one study showed incremental cost-effectiveness ratio of IV rtPA within 1 year was marginally above US$50000 per quality-adjusted life year threshold.IV rtPA within 0-3 hours after stroke led to cost savings for lifetime or 30 years and IV rtPA within 3-4.5 hours after stroke increased costs but still was cost-effective.Conclusions The literature generally showed that IV rtPA was a dominant or a cost-effective strategy compared with traditional treatment for patients with acute ischaemic stroke without IV rtPA.The findings from the literature lacked generalisability because of limited data and various assumptions. | Heesoo Joo Guijing Wang Mary G George | 2017 | Stroke & Vascular Neurology2017，2，2： | 2 |
| 3 | 胎盘生长因子抑制胎盘缺血引起的高血压显示文摘先兆子痫最有效的治疗是提前分娩出胎儿和胎盘。胎盘缺血的发生对先兆子痫发病有至关重要的影响,因为在动物或人类,子宫灌注压减少(reduced uterine perfusion pressure,RUPP)可诱发胎盘缺血/缺氧,刺激机体释放高血压胎盘因子进入母体循环。抗血管生成因子可溶性fms样酪氨酸激酶1(soluble fms-like tyrosine kinase-1,sFlt-1)可拮抗和减少有生物活性的血管内皮生长因子( vascular endothelial growth factor, VEGF)和胎盘生长因子( placental growth factor, PIGF),研究发现在RUPP大鼠和先兆子痫患者中,sFlt-1水平升高。 | 刘莉 叶鹏 Spradley FT Tan AY Joo WS Daniels G Kussie P Karumanchi SA Granger JP | 2016 | 中华高血压杂志2016，24，2： | 2 |
| 4 | The relationship of sarcoplasmic and myofibrillar protein solubility to colour and water- holding capacity in porcine longissimus muscle显示文摘 | JOO S T KAUFFMAN R G KIM B C | 1999 | Meat Science1999，52，3： | 1 |
| 5 | Development of high-driving potential and high-efficiency Mg-based sacrificial anodes for cathodic protection显示文摘 | KIM J G JOO J H KOO S J | 2000 | Journal of Materials Science Letters2000，19，6： | 1 |
| 6 | Recent advances in the homogeneous hydrogenation of carbon dioxide显示文摘 | Jessop P G Joo F Tai C C | 2004 | Coord Chem Rev2004，248，2124： | 1 |
| 7 | Prevalence of regulatory T cells is increased in peripheral blood and tumor microenvironment of patients with pancreas or breast adenocarcinoma显示文摘 | Liyanage U K Moore T T Joo H G | 2002 | J Immunol2002，169，： | 1 |
| 8 | Elevated MMP - 12 protein levels in induced sputum from patients with COPD 显示文摘 | Demedts I K Morel - Montero A Lebecque S Pacheco Y Cataldo D Joos G F Pauwels R A Brusselle G G | 2006 | Thorax2006，61，6： | 1 |
| 9 | The Phytoplankton Succession in the Lower Part of Hypertrophic Nakdong River (Mul- gum), South Korea显示文摘 | HA K KIM H JOO G | 1998 | Hydrobiologia1998，569，： | 1 |
| 10 | Allergen induced airway inflamma- tion and bronchial responsiveness in wildtype and interleukin - 4 - de- ficient mice 显示文摘 | Brusselle G Kips J Joos G | 1995 | Am J Respir Cell Mol Biol1995，12，4： | 1 |
| 11 | Performance investigation of a current-con- trolled voltage-regulated PMW rectifier in rotating in rotating and stationary frames 显示文摘 | ZARGARI N R JOOS G | 1995 | IEEE transactions on Industrial Electronics1995，42，4： | 1 |
| 12 | Robust Adaptive Control of Robot Manipulators Using Generalized Fuzzy Neural Networks显示文摘 | Joo E M Yang G | 2003 | IEEE Transactions on Industrial Electronics2003，50，6： | 1 |
| 13 | Serum neuron specific enolase as a tumor marker in the diagnosis and follow up of small cell lung cancer显示文摘 | Pinson P Joos G Watripont P | 2007 | Respiration2007，64，1： | 1 |
| 14 | A zero voltage transition Boost converter employing a soft switching auxiliary circuit with reduced conduction losses 显示文摘 | JAIN N JAIN K P JOOS G | 2004 | IEEE Transactions on Power Electronics2004，19，1： | 1 |
| 15 | DC-sice Shunt-active Power Filte for Phase-controlled Magnet-load Power Suplies显示文摘 | Wang Y Joos G Jin H | 1997 | IEEE Trans on Power Electronics1997，12，5： | 1 |
| 16 | Enhancement of electromagnetic interference shielding efficiency of polyaniline through mixture and chemical doping 显示文摘 | Joo J Lee C Y Song H G | | Molecular Crystals and Liquid Crystals Science and Technology0，，： | 1 |
| 17 | Effect of SO2 on mercury binding on carbonaceous surfaces显示文摘 | LIU J QU W JOO S W ZHENG C G | 2012 | Chem Eng J2012，184，： | 1 |
| 18 | Multi-object identification for mobile robot using ultrasonic sensors显示文摘 | Zhong SLim Soon T Kwon Moon G Joo | 2012 | International Journal of Control Automation and Systems2012，10，3： | 1 |
| 19 | Some peculiarities in the specific adsorption of phosphate ions on hematite and γ-Al2 O3 as reflected by ra- diotracer studies 显示文摘 | Horanyi G Joo P | 2002 | J Colloid Interface Sci2002，247，： | 1 |
| 20 | A hybrid structure using phase-controlled rectifiers and high-frequency converters for magnet-load power supplies显示文摘 | Jin H Wang Y Joos G | 1996 | IEEE Transactions on Industrial Electron1996，43，1： | 1 |
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