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| 1 | Pm21 from Haynaldia villosa Encodes a CC-NBS- LRR Protein Conferring Powdery Mildew Resistance in Wheat显示文摘 | Liping Xing Ping Hu Jiaqian Liu Kamil Witek Shuang Zhou Jiefei Xu Weihao Zhou Li Gao Zhenpu Huang Ruiqi Zhang Xiue Wang Peidu Chen Haiyan Wang Jonathan D.G. Jones Miroslava Karafiatova Jan Vrana Jan Baros Jaroslav Dolezel Yuanchun Tian Yufeng Wu Aizhong Cao | 2018 | Molecular Plant2018，11，6： | 16 |
| 2 | Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa,a wild relative of common wheat显示文摘Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the karyotype of H.villosa can be characterized is poor,hampering accurate characterization of small segmental alien introgressions.We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H.villosa chromosome 6 V(6 VS).FISH with seven of them resulted in clear signals on H.villosa chromosomes.Using these,we constructed FISH karyotypes for H.villosa using oligo-6 VS-1 and oligo-6 VS-35 oligonucleotides and characterized the distribution of the two probes in five different H.villosa accessions.The new FISH probes can efficiently characterize H.villosa introgressions into wheat. | Jia Lei Jiawen Zhou Haojie Sun Wentao Wan Jin Xiao Chunxia Yuan Miroslava Karafiátová Jaroslav Dolezel Haiyan Wang Xiue Wang | 2020 | The Crop Journal2020，8，4： | 3 |
| 3 | 基于纤毛鹅观草特异的卫星重复序列开发寡核苷酸探针显示文摘[目的]本文旨在开发纤毛鹅观草特异的寡核苷酸(oligonucleotide,oligo)探针,进一步完善纤毛鹅观草染色体鉴定技术。[方法]利用前期选育的普通小麦-纤毛鹅观草异附加系DA2S^(c)L和DA6S^(c),通过流式分拣和基因组二代测序获得纤毛鹅观草染色体6S^(c)和染色体臂2S^(c)长臂的基因组序列,利用Repeatexplorer2/TAREAN软件从中鉴定出卫星重复序列并设计成oligo探针,通过荧光原位杂交(fluorescence in situ hybridization,FISH)分析所开发oligo探针的应用价值。[结果]从6S^(c)和2S^(c)L基因组序列中共鉴定出11个卫星重复序列并开发成11个oligo探针,oligo-FISH结果表明,其中6个探针可以在纤毛鹅观草染色体上产生明显杂交信号,而在小麦染色体上未产生明显杂交信号,可用于特异鉴定小麦背景中的纤毛鹅观草染色体或片段。对1套普通小麦-纤毛鹅观草异附加系oligo-FISH分析发现,oligo-2S^(c)L^(-1)63和oligo-6S^(c)-111仅在S^(c)基因组染色体上产生明显信号,可作为纤毛鹅观草S^(c)组特异探针;将oligo-2S^(c)L-161和oligo-2S^(c)L-163组合,对1整套二体异附加系进行双色oligo-FISH,构建了纤毛鹅观草染色体的oligo-FISH核型。[结论]本研究提供了纤毛鹅观草重复序列组成的初步信息,开发的探针能特异鉴定纤毛鹅观草染色体,阐明纤毛鹅观草2个基因组的来源和分化,对推动纤毛鹅观草优异基因的转移和利用有重要意义。 | 程梦豪 Miroslava Karafiatova 孙昊杰 Kate Arina Holušva Jaroslav Dolezel 宋新颖 王海燕 王秀娥 | 2022 | 南京农业大学学报2022，45，3： | 2 |
| 4 | Two new nuclear isolation buffers for plant DNA flow cytometry: A Test with 37 Species 显示文摘 | JOAO LOUREIRO ELEAZAR RODRIGUEZ JAROSLAV DOLEZEL | 2007 | Annals of Botany2007，100，4： | 1 |
| 5 | Comparison of four nuclear isolation buffers for plant DNA flow cytometry 显示文摘 | JOAO LOUREIROI ELEAZAR RODRIGUEZI JAROSLAV DOLEZEL | 2006 | Annals of Botany2006，98，3： | 1 |
| 6 | Estimation of nuclear DNA content in plants using flow cytometry显示文摘 | Jaroslav Dolezel Johann Greilhuber | 2007 | Nature Protocols2007，2，9： | 1 |
| 7 | Sequence analysis of Erianthus arundinaceus chromosome 1 isolated by flow sorting after genomic in situ hybridization in suspension显示文摘Erianthus arundinaceus is a wild relative of sugarcane(Saccharum officinarum L.)with many desirable agronomic traits for sugarcane genetic improvement.However,limited knowledge of the complex genome of hexaploid E.arundinaceus has impeded the development of required molecular tools.Dissecting complex genomes into single chromosomes can simplify analyses.The flow-cytometric sorting of a single chromosome of E.arundinaceus in a Saccharum-Erianthus introgression line is reported.A novel approach called genomic in situ hybridization in suspension was used to discriminate the alien chromosome from sugarcane chromosomes at the same size.A total of 218,000 E.arundinaceus chromosome 1(EaC1)were sorted to>97%purity and amplified DNA was sequenced using Illumina and Pac Bio technologies.The resulting assembly had a 70.93 Mb contig sequence with an N50 of 19.62 kb.A total of 56.69 Mb repeat sequences were predicted,accounting for 79.1%of the chromosome and 2646 genes having a total length of 1.84 Mb that represented 2.59%of the chromosome.Of these genes,1877(70.9%)genes were functionally annotated.The phylogenetic relationship of E.arundinaceus with other species using the chromosome1 sequence revealed that E.arundinaceus was distantly related to Oryza sativa and Zea mays,followed by Sorghum bicolor,and was closely related to S.spontaneum and Saccharum spp.hybrids.This study provides the first insights into the characteristics of EaC1,and the results will provide tools to support molecular improvement and alien introgression breeding of sugarcane. | Shan Yang Petr Cápal Jaroslav Dolezel Xueting Li Wang Qian Zhiqiang Wang Kai Zeng Peiting Li Hongkai Zhou Rui Xia Muqing Zhang Zuhu Deng | 2022 | The Crop Journal2022，10，6： | 0 |
| 8 | Corrigendum to“Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat”[Crop J.11(2023)1512–1520]显示文摘The authors regret to report some missing information in the authorship and associated changes of the paper.On page 1512,the author information reads:“Caiyun Liu a,Wei Guo a,Yang Wang b,Bisheng Fu a,Jaroslav Dolezel c,Ying Liu a,Wenling Zhai a,Mahmoud Said c,d,István Molnár c,d,e,Katerina Holušovác,Ruiqi Zhang b,*,Jizhong Wu a,f,g,*”.It should be changed to“Caiyun Liu a,1,Wei Guo a,1,Yang Wang b,Bisheng Fu a,Jaroslav Dolezel c,Ying Liu a,Wenling Zhai a,Mahmoud Said c,d,István Molnár c,d,e,Katerina Holušovác,Ruiqi Zhang b,*,Jizhong Wu a,f,g,*”.On page 1512 in the footnote,the following sentence should be added below the corresponding authors’information:1 These authors contributed equally to this work.The correction has been updated above.The authors would like to apologize for any inconvenience caused. | Caiyun Liu Wei Guo Yang Wang Bisheng Fu Jaroslav Dolezel Ying Liu Wenling Zhai Mahmoud Said István Molnár Katerina Holušová Ruiqi Zhang Jizhong Wu | 2023 | The Crop Journal2023，11，6： | 0 |
| 9 | Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing显示文摘The large genome size (~17000 Mb) and complicated DNA structures of common wheat (Triticum aestivum) hamper its genome sequencing.By means of flow cytometry,systematic investigations on individual chromosome sorting have been carried out to construct chromosome-specific bacterial artificial chromosome (BAC) libraries since the 1980s.Several wheat chromosome-specific BAC libraries,such as chromosome 3B,three D genome chromosomes (1D,4D and 6D),and the short arm of chromosome 1B,have been developed,and the physical map of chromosome 3B was established in 2008.The same chromosome-based strategy is being employed by the International Wheat Genome Sequencing Consortium (IWGSC) to establish the physical maps of the other 20 common wheat chromosomes (cv.Chinese Spring).Several projects on wheat genome sequencing are currently underway.The availability of new sequencing technologies provides new choices for sequencing of gene space of the wheat genome.The applications of flow-sorted chromosomes in wheat genome studies present some examples to analyze the complex genomes of cereals. | WU SuoWei XIAO Yang ZHENG Xu CAI YingFan DOLEZEL Jaroslav LIU Bing Hua YANG Li SONG MeiFang ZHOU Peng ZHOU Yang MENG FanHua WANG ShanHong LIU HongWei HAI HuQu YANG JianPing | 2010 | Chinese Science Bulletin2010，55，15： | 0 |
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