|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Modified technique of Wolbachia removal from Malaysian Aedes albopictus显示文摘Objective:To develop an artificial and modified Wolbaehia removal technique using tetracycline from naturally Wolbachia infected Aedes albopictus(Ae.albopictus)so as to be able to produce generations of Wolbaehia free offsprings.Methods:In this study,seven different tetracycline treatment methods were conducted to obtain the best removal method.Four methods focused on larvae tetracycline treatment,one method on both larvae and adult tetracycline treatment and the last two methods on adult mosquito sucrose treatment.Results:All larval tetracycline treatments resulted in either high larvae mortality,sterile F_o adult mosquitoes or unsuccessful Wolbaehia removal.Treatment of both larvae and adults resulted in reduced larvae mortality,successful Wolbachia removal but slow mosquito fecundity.As for the adult treatment,1.0 mg/mL as previously published was not aisle to completely remove Wolbaehia in F,generation whereas 1.25 mg/mL successfully removed Wolbachia from F,and F,mosquitoes in 2 weeks.Conclusions:This method is different from the previously published methods as it provides an improved Watbachia removal technique from Ae.albopictus with high egg hatchability.low larvae mortality,increased fecundity and better Wolbaehia removal rate. | Sylvia Joanne Indra Vythilingam Nava Yugavathy Jonathan Inbaraj Doss | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，7： | 0 |
| 2 | Comparison of microscopy and PCR for the detection of human Plasmodium species and Plasmodium knowlesi in southern Myanmar显示文摘Objectives: To determine the distribution of Plasmodium(P) species including Plasmodium knowlesi and to compare the specificity and sensitivity of microscopy with nested PCR in malaria diagnosis.Methods: The study was conducted in Kawthaung, southern Myanmar. Ninety clinically suspected malaria patients were screened for malaria by Giemsa stained microscopy and con firmed by nested PCR.Results: Among the participants, 57(63.3%) were positive and 33(36.7%)were negative by microscopy. Of positive samples, 39(68.4%) were Plasmodium fcalciparum,17(29.8%) Plasmodium vivax and 1(1.8%) Plasmodium malariae, whereas 59-amplified by PCR were 40(67.8%), 18(30.5%) and 1(1.7%) respectively. PCR amplified 2 microscopy negative samples. Two samples of P. falciparum detected by microscopy were amplified as P. vivax and vice versa. All samples were negative for Plasmodium ovale.P. knowlesi and mixed infections. Microscopy had a very good measure of agreement(κ=0.95) compared to nested PCR. Sensitivity and specificity of microscopy for diagnosis of P. falciparum were 92.5%(95% CI: 79.6-98.4) and 96.0%(95% CI: 86.3-99.5)respectively, whereas for P. vivax were 83.3%(95% CI: 58.6-96.4) and 97.2%(95% CI:90.3-99.7).Conclusions: P. knowlesi was not detected by both microscopy and PCR. Giemsa stained microscopy can still be applied as primary method for malaria diagnosis and is considered as gold standard. As to the lower sensitivity of microscopy for vivax malaria,those with previous history of malaria and relapse cases should be diagnosed by RDT or PCR combined with microscopy. Inaccuracy of species diagnosis highlighted the requirement of training and refresher courses for microscopists. | Thu Zar Han Kay Thwe Han Kyin Hla Aye Thaung Hlaing Kyaw Zin Thant Indra Vythilingam | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，8： | 0 |
| 3 | Gravid oviposition sticky trap and dengue non-structural 1 antigen test for early surveillance of dengue in multi-storey dwellings: study protocol of a cluster randomized controlled trial显示文摘Background:Dengue is a global disease,transmitted by the Aedes vectors.In 2018,there were 80615 dengue cases with 147 deaths in Malaysia.Currently,the nationwide surveillance programs are dependent on Aedes larval surveys and notifications of lab-confirmed human infections.The existing,reactive programs appear to lack sensitivity and proactivity.More efficient dengue vector surveillance/control methods are needed.Methods:A parallel,cluster,randomized controlled,interventional trial is being conducted for 18 months in Damansara Damai,Selangor,Malaysia,to determine the efficacy of using gravid oviposition sticky(GOS)trap and dengue non-structural 1(NS1)antigen test for early surveillance of dengue among Aedes mosquitoes to reduce dengue outbreaks.Eight residential apartments were randomly assigned into intervention and control arms.GOS traps are set at the apartments to collect Aedes weekly,following which dengue NS1 antigen is deteaed in these mosquitoes.When a dengue-positive mosquito is detected,the community will be advised to execute vector search-and-destroy and protective measures.The primary outcome concerns the the percentage change in the(i)number of dengue cases and(ii)durations of dengue outbreaks.Whereas other outcome measures include the change in density threshold of Aedes and changes in dengue-related knowledge,attitude and practice among cluster inhabitants.Discussion:This is a proactive and early dengue surveillance in the mosquito vector that does not rely on notification of dengue cases.Surveillance using the GOS traps should be able to efficiently provide sufficient coverage for multistorey dwellings where population per unit area is likely to be higher.Furthermore,trapping dengue-infected mosquitoes using the GOS trap,helps to halt the dengue transmission carried by the mosquito.It is envisaged that the results of this randomized controlled trial will provide a new proactive,cheap and targeted surveillance tool for the prevention and control of dengue outbreaks.Trial registration:This is a parallel-cluster,randomized controlled,interventional trial,registered at ClinicalTrials.gov(ID:NCT03799237),on 8th January 2019(retrospectively registered). | Jonathan Wee Kent Liew Sivaneswari Selvarajoo Wing Tan Rafdzah Ahmad Zaki Indra Vythilingam | 2019 | Infectious Diseases of Poverty2019，8，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员