|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Vascular endothelial growth factor 165b expression in stromal cells and colorectal cancer显示文摘AIM:To characterize the implications of vascular endothelial growth factor(VEGF)-A in stromal cells and colorectal cancer and the expression of VEGF-A splice variants.METHODS:VEGF-A expression in tumor and stromal cells from 165 consecutive patients with colorectal cancer was examined by immunohistochemistry.The association between VEGF-A expression status and clinicopathological factors was investigated.Twenty freshfrozen samples were obtained for laser capture microdissection to analyze the splice variants of VEGF-A.RESULTS:VEGF-A was expressed in 53.9% and 42.4% of tumor and stromal cells,respectively.VEGF-A expression in tumor cells(t-VEGF-A) was associated with advanced clinical stage(stage 0,1/9;stage 1,2/16;stage 2,32/55;stage 3,38/66;stage 4,16/19,P < 0.0001).VEGF-A expression in stromal cells(s-VEGF-A) increased in the earlier clinical stage(stage 0,7/9;stage 1,6/16;stage 2,33/55;stage 3,22/66;stage 4,5/19;P = 0.004).Multivariate analyses for risk factors of recurrence showed that only s-VEGF-A expression was an independent risk factor for recurrence(relative risk 0.309,95% confidence interval 0.141-0.676,P = 0.0033).The five-year disease-free survival(DFS) rates of t-VEGF-A-positive and-negative cases were 51.4% and 62.9%,respectively.There was no significant difference in t-VEGF-A expression status.The five-year DFS rates of s-VEGF-A-positive and-negative cases were 73.8% and 39.9%,respectively.s-VEGFA-positive cases had significantly better survival than s-VEGF-A-negative cases(P = 0.0005).Splice variant analysis revealed that t-VEGF-A was mainly composed of VEGF165 and that s-VEGF-A included both VEGF165 and VEGF165b.In cases with no venous invasion(v0),the level of VEGF165b mRNA was significantly higher(v0 204.5 ± 122.7,v1 32.5 ± 36.7,v2 2.1 ± 1.7,P = 0.03).The microvessel density tended to be lower in cases with higher VEGF165b mRNA levels.CONCLUSION:s-VEGF-A appears be a good prognostic factor for colorectal cancer and includes VEGF165 and VEGF165b. | Makoto Tayama Tomohisa Furuhata Yoshiko Inafuku Kenji Okita Toshihiko Nishidate Toru Mizuguchi Yasutoshi Kimura Koichi Hirata | 2011 | World Journal of Gastroenterology2011，17，44： | 10 |
| 2 | Phosphorylation of alphaB-crystallin in epiretinal membrane of human proliferative diabetic retinopathy显示文摘AIM: To examine phosphorylation of alpha B-crystallin(p-αBC), a vascular endothelial growth factor(VEGF)chaperone, and immunohistochemically investigate relationship between p-αBC, VEGF and phosphorylated p38-mitogen-activated protein kinase(p-p38 MAPK) in the epiretinal membrane of human proliferative diabetic retinopathy(PDR).METHODS: Eleven epiretinal membranes of PDR surgically excised were included in this study. Two normal retinas were also collected from enucleation tissues due to choroidal melanoma. Paraformaldehyde-fixed, paraffin-embedded tissue sections were processed for immunohistochemistry with anti-p-αBC, VEGF, CD31,and p-p38 MAPK antibodies.RESULTS: Immunoreactivity for p-αBC was observed in all of the epiretinal membranes examined, where phosphorylation on serine(Ser) 59 showed strongest immunoreactivity in over 70% of the membranes. The immunolocalization of p-αBC was detected in the CD31-positive endothelial cells, and co-localized with VEGF and p-p38 MAPK in PDR membranes. Immunoreactivity for p-αBC, however, was undetectable in endothelial cells of the normal retinas, where p-p38 MAPK immunoreactivity was less marked than PDR membranes.CONCLUSION: Phosphorylation of αBC, in particular,phosphorylation on Ser59 by p-p38 MAPK may play a potential role as a molecular chaperon for VEGF in the pathogenesis of epiretinal membranes in PDR. | Yoko Dong Zhenyu Dong Satoru Kase Ryo Ando Junichi Fukuhara Satoshi Kinoshita Saori Inafuku Yoshiaki Tagawa Erdal Tan Ishizuka Wataru Saito Miyuki Murata Atsuhiro Kanda Kousuke Noda Susumu Ishida | 2016 | International Journal of Ophthalmology(English edition)2016，9，8： | 4 |
| 3 | Poten preventive action of curcuminonradiation-induced initiation of mammary tumorigrnesis in rats 显示文摘 | Inaro H Onada M Inafuku N | 2000 | Carcinogenesis2000，21，： | 1 |
| 4 | Effect of aging on the expression,NOS and cell death in the mouse cochlear spiral ganglion显示文摘 | Wu MD Kimura M Inafuku S | 1997 | Okajimas Folia Anat Jpn1997，74，5： | 1 |
| 5 | Potent preventive action of curcumin on radiation -induced initiation of mammary tumouigenesis in rats 显示文摘 | inano H Onoda M Inafuku N | 2000 | Carcinogenesis2000，21，10： | 1 |
| 6 | Potent preventive action of curcumin on radiation-induced initiation of mammary tumorigenesis in rats显示文摘 | Inaro H Onada M Inafuku N | 2000 | Carcinogenesis2000，21，： | 1 |
| 7 | Hysteroscopic assessment of midsecretory-phase endometrium, with special relerence to the luteal-phase defect 显示文摘 | Sakumoto T Inafuku K Miyara M | 1992 | Horm Res1992，37，1： | 1 |
| 8 | Ultras onographic evaluation of lower uterine segment to predict the integrity and quality of ce- sarean scar during pregnancy: aprosepec tive study显示文摘 | Qureshi Inafuku K Qshima | 1997 | Tohoku J Exp Med1997，183，： | 1 |
| 9 | Effect of aging onthe expression of iNOS and cell death in the mouse co- chlear spiral ganglion显示文摘 | Wu MD Kimura M Inafuku S | 1997 | Okajimas Folia Anat Jpn1997，74，5： | 1 |
| 10 | Mukitake mushroom (Panellus serotinus)alleviates nonalcoholic fatty liver disease through the suppression of monocyte chemoattractant protein 1 production in db/db mice显示文摘 | Nagao K Inoue N Inafuku M | | 0，，05： | 1 |
| 11 | lzotent preventive action of curcumin on radiation-induced initiation of mammary tumorigenesis in rats显示文摘 | Inano H Onoda M Inafuku N | 2000 | Carcinogenesis2000，21，10： | 1 |
| 12 | Aging and superoxide dismutase activity in cerebrospinal fluid显示文摘 | Okabe T Hamaguchi K Inafuku T | 1996 | J Neuro Sci1996，141，12： | 1 |
| 13 | A case of postmenopausal breast cancer becoming HER2 positive after neoadjuvant hormone therapy显示文摘 | Inafuku K Inari H Goto N | 2014 | Gan To Kagaku Ryoho2014，41，4： | 1 |
| 14 | Determination of oxidative stress and cardiac dysfunction after ischemia/reperfusion injury in isolated rat hearts 显示文摘 | Inafuku H Kuniyoshi Y Yamashiro S | 2013 | Ann Thorac Cardiovasc Surg2013，19，3： | 1 |
| 15 | Ultrasonographic evaluation of lower uterine segment to predict the integrity and quality of cesarean scar during pregnancy:a prosepective study显示文摘 | Quresh I Inafuku K Qshim A | 1997 | Tohoku J Exp Med1997，183，1： | 1 |
| 16 | Effect of aging on the expression ,NOS and cell death in the mouse cochlear spiral agnglion显示文摘 | Wu MD Kimura M Inafuku S | 1997 | Okajimas Folia Anat Jpn1997，74，5： | 1 |
| 17 | Potent preventive action of curcumin on radiation-induced initiation of mammary tumorigenesis in rats显示文摘 | INANO H ONODA M INAFUKU N | 2000 | Carcinogenesis2000，21，10： | 1 |
| 18 | Ultrasonographic evaluation of lower uter-ine segment to predict the integrity and quality of cesarean'scar during preg-nancy:a prosepective study显示文摘 | Qureshi Inafuku K Qshima | 1997 | Tohoku J Exp Med1997，183，78： | 1 |
| 19 | Chitobiase activity as an indi- cator of altered survival, growth and reproduction in Daphniapulex and Daphnia magna( Crustacea : Cladocera )exposed to spinosad and di- flubenzuron显示文摘 | Duchet C Inafuku M M Caquet T | 2011 | Ecotoxicology and Environmental Safety2011，74，4： | 1 |
| 20 | Potent preventive action of curcumin on radiation-induced initiation of mammary tumorigenesis in rata显示文摘 | Inano H Onada M Inafuku N et at | 2000 | Carcinogenesis2000，21，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员