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| 1 | Clinically relevant human temporal bone measurements using novel high-resolution cone-beam CT显示文摘Objective: To test the feasibility of measuring fine temporal bone structures using a newly established cone-beam computed tomography(CBCT)system.Materials and methods: Six formalin-fixed human cadaver temporal bones were imaged using a high-resolution CBCT system that has 900 frames and copper t aluminum filtration. Fine temporal bone structures, including those of the facial nerve canal and vestibular structures, were identified and measured.Results: The fine structures of the middle ear, including the tympanic membrane, tendon of the tensor tympani, cochleariform process of the semicanal of the tensor tympani, pyramidal eminence, footplate of the stapes, full path of the facial nerve within the temporal bone, supralabyrinthine space, semicircular canals, pathway of the subarcuate canal, and full path of the vestibular aqueduct, were clearly demonstrated. The vestibular aqueduct has a midpoint width of 0.4 ± 0.0 mm and opercular width of 0.5 ± 0.1 mm(mean ± SD). The length of the internal acoustic meatus was 10.6 ± 1.2 mm(mean ± SD), and the diameter of the internal acoustic meatus was 3.7 ± 0.3 mm(mean ± SD).Conclusion: This novel high-resolution CBCT system has potentially broad applications in the diagnosis of inner ear disease and in monitoring associated pathological changes, surgical planning, navigation for the ear surgery, and temporal bone training. | Jing Zou Jaakko Laahelma Antti Arnisalo Ilmari Pyykko | 2017 | Journal of Otology2017，12，1： | 10 |
| 2 | Label-free visualization of cholesteatoma in the mastoid and tympanic membrane using CARS microscopy显示文摘Objective:The present study aimed to evaluate the possibility of using coherent anti-Stokes Raman spectroscopy(CARS) microscopy to determine the specific molecular morphology of cholesteatoma by detecting the natural vibrational contrast of the chemical bonds without any staining.Materials and methods:Specimens from the mastoid and tympanic membrane with and without cholesteatoma were analyzed using CARS microscopy,two-photon excited fluorescence(TPEF) microscopy,and the second harmonic generation(SHG) microscopy.Results:In cholesteatoma tissues from the mastoid,a strong resonant signal at 2845 cm^(-1) was observed by CARS,which indicated the detection of the CH_2 hydro-carbon lipid bonds that do not generate visible signals at 2940 cm^(-1) suggestive of CH_3 bonds in amino acids.A strong resonant signal at 2940 cm^(-1) appeared in an area of the same specimen,which also generated abundant signals by TPEF and SHG microscopy at 817 nm,which was suggestive of collagen.In the tympanic membrane specimen with cholesteatoma,a strong resonant signal with corrugated morphology was detected,which indicated the presence of lipids.A strong signal was detected in the tympanic membrane with chronic otitis media using TPEF/SHG at 817 nm,which indicated collagen enrichment.The CARS and TPEF/SHG images were in accordance with the histology results.Conclusion:These results suggest the need to develop a novel CARS microendoscope that can be used in combination with TPEF/SHG to distinguish cholesteatoma from inflammatory tissues. | Jing Zou Antti Isomaki Timo Hirvonen Antti Aarnisalo Jussi Jero Ilmari Pyykko | 2016 | Journal of Otology2016，11，3： | 4 |
| 3 | 线粒体毒素诱导伴有眩晕的突发性耳聋模型的建立显示文摘目的建立一个突发性感音神经性耳聋(SSNHL)的动物模型,以研究其发病机制和治疗方法。方法49只雄性豚鼠根据给药浓度和听性脑干反应(ABR)测试时间分为7组,用浸有0.3、0.5 mol/L3-硝基丙酸(3-NP)或磷酸盐缓冲液(PBS)的明胶海绵分别放置于豚鼠左耳圆窗龛,作用30 min后取出。4%多聚甲醛加1%戊二醛行活体心脏灌注固定,耳蜗JB4包埋、切片后做光镜观察。结果所有暴露于3-NP的动物均表现中、重度听力减退至ABR反应消失。高剂量(0.5 mol/L 3-NP)组动物均出现快相指向健侧的自发性眼震和不同程度的姿势失衡等单侧前庭功能损伤的典型症状;低剂量(0.3 mol/L3-NP)组仅1只动物出现自发性眼震,2只动物出现姿势失衡。前庭症状在暴露后2 d内消失。所有实验组动物柯蒂氏器和支持细胞出现一过性水肿。暴露后12 h,螺旋神经节细胞胞浆和胞核内有空泡形成,第7天螺旋神经节细胞变性。结论经圆窗膜投放3-NP至内耳可以复制出SSNHL的临床症状,螺旋神经节细胞损伤是其最明显的病理改变。 | 张亚 邹静 Ilmari Pyykko 吴皓 | 2007 | 上海交通大学学报(医学版)2007，27，1： | 4 |
| 4 | 豚鼠外源性血管内皮细胞生长因子增强内耳屏障转运作用的磁共振成像研究显示文摘目的观察血管内皮细胞生长因子(vascularendothelialgrowthfactor, VEGF)能否改变血迷路屏障和血外淋巴屏障的物质转运作用。方法11只300 ~900g雌雄兼并的杂色豚鼠在甲苯噻嗪(16mg/kg)和氯氨酮(60mg/kg)基础麻醉下接受手术,通过圆窗膜(明胶海绵吸附)将VEGF(6耳)或磷酸盐缓冲液(PBS, 5耳)投放至内耳。用T1对比剂二乙烯三胺五乙酸双甲酰胺钆(gadolinium diethylenetriamine pentaacetate bismethylamide, Gd DTPA BMA)作为内耳屏障转运示踪剂,用4 7T场强, 40cm孔径的BrukerBiospecAvance47 /40试验磁共振系统进行豚鼠耳蜗二维磁共振成像观测,用Paravision软件进行图像密度分析,用AdobePhotoshop6 0软件进行图像呈示。结果圆窗膜投放PBS未影响血外淋巴屏障渗透性变化。圆窗膜投放VEGF可显著增强处理侧血外淋巴屏障渗透性,VEGF处理的耳蜗鼓阶内Gd DTPA BMA转运显著增加(P<0 01 ), VEGF处理的耳蜗前庭阶内Gd DTPA BMA转运也显著增加(P<0 01)。VEGF处理的耳蜗中阶内Gd DTPA BMA转运无明显增加(P>0 05)。结论VEGF显著增强血外淋巴屏障的物质转运作用并可能有利于内耳在各种有害环境下的代偿及修复。 | 邹静 Pyykko Ilmari Bjelke Borje Counter S Allen | 2005 | 中华耳鼻咽喉头颈外科杂志2005，40，4： | 3 |
| 5 | Dexamethasone does not affect endolymphatic hydrops(EH)in patients with Meniere's disease within 24 h,and intratympanic administration of gadolinium plus dexamethasone simplifies high-quality imaging of EH using a novel protocol of 7 min显示文摘Background:Intratympanic administration of gadolinium chelate allows for a better visualization of endolymphatic hydrops(EH)using MRI than intravenous injection and was recently further improved to facilitate high-quality imaging of EH in 7 min.The aim of the present study was to simplify the intratympanic administration protocol by mixing gadolinium chelate with therapeutic dexamethasone and to evaluate the effects of this mixture on the visualization of EH in MRI.Materials and methods:In an in vitro study,the potential impact of gadolinium-diethylenetriamine pentaacetic acid(Gd-DTPA)on the stability of dexamethasone was evaluated by analyzing dynamic changes in dexamethasone with high-performance liquid chromatography(HPLC)after mixing with GdDTPA.Ten patients with definite Meniere's disease(MD)were recruited to study the potential interference of dexamethasone on MRI visualization of EH,and 49 patients with MD were recruited to evaluate the effect of intratympanic injection of Gd-DTPA mixed with dexamethasone on MRI of EH using a 3T MR machine and a novel heavily T2-weighted 3-dimensional fluid-attenuated inversion recovery reconstructed using a magnitude plus zero-filled interpolation(hT2FLAIR-MZFI)sequence.Results:The retention times and peak area of dexamethasone in HPLC were not modified by the addition of Gd-DTPA.EH grading in the cochlea and vestibule was not influenced in any ear by intratympanic injection of dexamethasone.Excellent inner ear images were obtained from all patients,and EHs with various grades were displayed.There were significant correlations between diagnosis and cochlear EH(p<0.01,Spearman's Rho),between diagnosis and vestibular EH(p<0.01,Spearman's Rho),and between cochlear and vestibular EH(p<0.01,Spearman's Rho).The distribution of Gd-DTPA plus dexamethasone negatively correlated with the grade of vestibular EH.Injury of the endolymph-perilymph barrier was detected in one cochlea and three vestibules of 59 inner ears with MD.Conclusions:Intratympanic administration of Gd-DTPA plus dexamethasone yielded high-quality MRI images of EH in patients with MD using a novel 7-min protocol and simplified the clinical application.Intratympanic administration of Gd-DTPA plus dexamethasone might be used to test its therapeutic effect in future work. | Jing Zou Zikai Zhao Hongbin Li Guoping Zhang Qing Zhang Jianping Lu Ilmari Pyykko | 2022 | Journal of Otology2022，17，3： | 2 |
| 6 | MEFV,IRF8,ADA,PEPD,and NBAS gene variants and elevated serum cytokines in a patient with unilateral sporadic Meniere's disease and vascular congestion over the endolymphatic sac显示文摘The etiology and underlying mechanism of Meniere's disease(MD)development are still unknown,although inflammation and autoimmunity have been implicated as underlying mechanisms.The human endolymphatic sac(ES)has been reported to have innate and adaptive immune capacity in local immune reactions.In vivo demonstration of inflammation of the ES in patients with MD is missing in the literature.We report the case of a 47-year-old female patient diagnosed with unilateral MD with genetic variants and cytokine markers indicating inflammation and vascular congestion of the ES.Endolymphatic hydrops in the right cochlea(grade 2)and vestibulum(grade 3)were detected using MRI.She carried heterozygous variants in MEFV(c.442G>C),IRF8(c.1157G>T),ADA(c.445C>T),PEPD(c.151G>A),NBAS(c.4049T>C),CSF2RB(c.2222C>T),HPS6(c.277G>T),IL2RB(c.1109C>T),IL12RB1(c.1384G>T),IL17RC(c.260_271del GCAAGAGC TGGG),LIG1(c.746G>A),RAG1(c.650C>A),and SLX4(c.1258G>C,c.5072A>G).In the serum,the levels of granulocyte colony-stimulating factor(G-CSF),macrophage inflammatory protein 1a,and IL7 were significantly elevated,and the level of IL2Ra was reduced.Intratympanic administration of dexamethasone temporarily alleviated her hearing loss.Her vertigo was significantly relieved but remained slight after ES administration of corticosteroids. | Jing Zou Zikai Zhao Guoping Zhang Qing Zhang Ilmari Pyykko | 2022 | Journal of Otology2022，17，3： | 0 |
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