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| 1 | 鸭子α-干扰素基因表达及多样性分析显示文摘目的 :采用分子杂交及PCR方法 ,分析鸭α 干扰素基因的表达及多态性。方法 :从鸭外周血分离出的单核细胞在体外经PHA(5 μg/ml)刺激不同时间后 ,提取总RNA。以RT PCR方法检测鸭α 干扰素 (DuIFN α)mRNA表达状况。引物根据最近公布的DuIFN α基因序列设计。从鸭外周血单核细胞中提取的基因组DNA经限制性内切酶BamHI,HindIII,PstI,XbaI消化后 ,以公布的DuIFN α序列为探针 ,采用Southern杂交分析DuIFN α基因的多样性。结果 :在未经PHA刺激的鸭外周血单核细胞 (PBMCs)中 ,未检测到DuIFN α表达 ;PHA刺激 4h后 ,即可检测到DuIFN α表达 ,一直持续到 2 4h。基因组DNA限制性内切酶多态性分析表明 ,PstI酶切后 ,出现片段大小各异的杂交信号 ,提示DuIFN α存在多样性。结论 :鸟类α 干扰素基因与哺乳动物类似 。 | 黄爱龙 AllisonJ ilbert Ieva Kotlarski | 2000 | 中国免疫学杂志2000，16，12： | 4 |
| 2 | 鸭γ-干扰素活性体外检测方法的建立显示文摘目的:建立鸭γ-干扰素体外活性检测方法。方法:体外刺激巨噬细胞产生一氧化氮(NO)是γ-干扰素的生物学效应之一。为检测鸭 γ-干扰素活性,采用 Sephadex G-100诱导产生的腹腔巨噬细胞作为实验体系,观察一氧化氮浓度在LPS,PHA,L-精氨酸刺激的上述细胞中的变化情况。结果:结果表明上述方法制备的腹腔巨噬细胞经PHA或LPS活化后,细胞密度较高组一氧化氮产量明显升高,细胞密度较低组则没有显著差异。结论:高浓度的腹腔巨噬细胞对LPS等刺激物敏感,可作为检测鸭γ-干扰素活性的重要手段。 | 单幼兰 黄爱龙 Allison Jilbert Ieva Kotlarski | 2001 | 重庆医科大学学报2001，26，4： | 3 |
| 3 | Liver cirrhosis and left ventricle diastolic dysfunction: Systematic review显示文摘BACKGROUND Liver cirrhosis is a chronic hepatic disease which is associated with cardiovascular abnormalities.Hyperdynamic circulation in liver cirrhosis causes functional and structural cardiac alterations.The prevalence of left ventricle diastolic dysfunction(LVDD)in cirrhotic patients ranges from 25.7%to as high as 81.4%as reported in different studies.In several studies the severity of diastolic dysfunction(DD)correlated with a degree of liver failure and the rate of dysfunction was higher in patients with decompensated cirrhosis compared with compensated.Future directions of comprehensive assessment of cardiac function in cirrhotic patients might provide a better prognosis for these patients.AIM To clarify the correlation between the severity of liver cirrhosis and left ventricle diastolic dysfunction in the existing literature.METHODS Through January and February of 2019 at Vilnius University we conducted a systematic review of the global existing literature on the prevalence of left ventricle diastolic dysfunction in patients with liver cirrhosis.We searched for articles in PubMed,Medline and Web of science databases.Articles were selected by using adequate inclusion and exclusion criteria.Our interest was the outcome of likely correlation between the severity of cirrhosis[evaluated by Child-Pugh classes,Model For End-Stage Liver Disease(MELD)scores]and left ventricle diastolic dysfunction[classified according to American Society of Echocardiography(ASE)guidelines(2009,2016)],as well as relative risk of dysfunction in cirrhotic patients.Subgroup analyses were performed to evaluate the ratio and grades of left ventricle diastolic dysfunction with respect to cirrhosis severity.RESULTS A total of 1149 articles and abstracts met the initial search criteria.Sixteen articles which met the predefined eligibility criteria were included in the final analysis.Overall,1067 patients(out of them 723 men)with liver cirrhosis were evaluated for left ventricle diastolic dysfunction.In our systemic analysis we have found that 51.2%of cirrhotic patients had left ventricle diastolic dysfunction diagnosed and the grade 1 was the most prevalent(59.2%,P<0.001)among them,the grade 3 had been rarely diagnosed-only 5.1%.The data about the prevalence of diastolic dysfunction in cirrhotic patients depending on Child-Pugh Classes was available from 5 studies(365 patients overall)and only in 1 research diastolic dysfunction was found being associated with severity of liver cirrhosis(P<0.005).We established that diastolic dysfunction was diagnosed in 44.6%of Child-Pugh A class patients,in 62%of Child B class and in 63.3%of Child C patients(P=0.028).The proportion of patients with higher diastolic dysfunction grades increases in more severe cirrhosis presentation(P<0.001).There was no difference between mean MELD scores in patients with and without diastolic dysfunction and in different diastolic dysfunction groups.In all studies diastolic dysfunction was more frequent in patients with ascites.CONCLUSION This systemic analysis suggests that left ventricle diastolic dysfunction is an attribute of liver cirrhosis which has not received sufficient attention from clinicians so far.Future suggestions of a comprehensive assessment of cardiac function in cirrhotic patients might provide a better prognosis for these patients and give hint for better understanding of the left ventricle diastolic dysfunction pathogenesis in liver cirrhosis. | Ieva Stundiene Julija Sarnelyte Ausma Norkute Sigita Aidietiene Valentina Liakina Laura Masalaite Jonas Valantinas | 2019 | World Journal of Gastroenterology2019，25，32： | 3 |
| 4 | Connective tissue growth factor reacts as an IL-6/STAT3-regulated hepatic negative acute phase protein显示文摘AIM:To investigate the mechanisms involved in a possible modulator role of interleukin(IL) -6 signalling on CYR61-CTGF-NOV(CCN) 2/connective tissue growth factor(CTGF) expression in hepatocytes(PC) and to look for a relation between serum concentrations of these two parameters in patients with acute inflammation. METHODS:Expression of CCN2/CTGF,p-STAT3,p-Smad 3/1 and p-Smad2 was examined in primary freshly isolated rat or cryo-preserved human PC exposed to various stimuli by Western blotting,electrophoretic mobility shift assay(EMSA) ,reporter-gene-assays and reversetranscriptase polymerase chain reaction. RESULTS:IL-6 strongly down-regulated CCN2/CTGF protein and mRNA expression in PC,enhanceable by extracellular presence of the soluble IL-6 receptor gp80,and supported by an inverse relation between IL-6 and CCN2/CTGF concentrations in patients'sera.The inhi-bition of TGFβ1 driven CCN2/CTGF expression by IL-6 did not involve a modulation of Smad2(and Smad1/3) signalling.However,the STAT3 SH2 domain binding peptide,a selective inhibitor of STAT3 DNA binding activity,counteracted the inhibitory effect of IL-6 on CCN2/CTGF expression much more pronounced than pyrrolidine-dithiocarbamate,an inhibitor primarily of STAT3 phosphorylation.An EMSA confirmed STAT3 binding to the proposed proximal STAT binding site in the CCN2/CTGF promoter. CONCLUSION:CCN2/CTGF is identified as a hepatocellular negative acute phase protein which is downregulated by IL-6 via the STAT3 pathway through interaction on the DNA binding level. | Olav A Gressner Ieva Peredniene Axel M Gressner | 2011 | World Journal of Gastroenterology2011，17，2： | 3 |
| 5 | Gut microbiota contribution to hepatocellular carcinoma manifestation in non-alcoholic steatohepatitis显示文摘Recently,the gut microbiota has been recognized as an obvious active player in addition to liver steatosis/steatohepatitis in the pathophysiological mechanisms of the development of hepatocellular carcinoma(HCC),even in the absence of cirrhosis.Evidence from clinical and experimental studies shows the association of specific changes in the gut microbiome and the direct contribution to maintaining liver inflammation and/or cancerogenesis in nonalcoholic fatty liver disease-induced HCC.The composition of the gut microbiota differs significantly in obese and lean individuals,especially in the abundance of pro-inflammatory lipopolysaccharide-producing phyla,and,after establishing steatohepatitis,it undergoes minor changes during the progression of the disease toward advanced fibrosis.Experimental studies proved that the microbiota of obese subjects can induce steatohepatitis in normally fed mice.On the contrary,the transplantation of healthy microbiota to obese mice relieves steatosis.However,further studies are needed to confirm these findings and the mechanisms involved.In this review,we have evaluated well-documented clinical and experimental research on the role of the gut microbiota in the manifestation and promotion of HCC in nonalcoholic steatohepatitis(NASH).Furthermore,a literature review of microbiota alterations and consequences of dysbiosis for the promotion of NASH-induced HCC was performed,and the advantages and limitations of the microbiota as an early marker of the diagnosis of HCC were discussed. | Valentina Liakina Sandra Strainiene Ieva Stundiene Vaidota Maksimaityte Edita Kazenaite | 2022 | World Journal of Hepatology2022，14，7： | 2 |
| 6 | Coordinated AR and microRNA regulation in prostate cancer显示文摘The androgen receptor(AR)remains a key driver of prostate cancer(PCa)progression,even in the advanced castrate-resistant stage,where testicular androgens are absent.It is therefore of critical importance to understand the molecular mechanisms governing its activity and regulation during prostate tumourigenesis.MicroRNAs(miRs)are small w22 nt noncoding RNAs that regulate target gene,often through association with 30 untranslated regions(30UTRs)of transcripts.They display dysregulation during cancer progression,can function as oncogenes or tumour suppressors,and are increasingly recognised as targets or regulators of hormonal action.Thus,understanding factors which modulate miRs synthesis is essential.There is increasing evidence for complex and dynamic bi-directional cross-talk between the multi-step miR biogenesis cascade and the AR signalling axis in PCa.This review summarises the wealth of mechanisms by which miRs are regulated by AR,and conversely,how miRs impact AR’s transcriptional activity,including that of AR splice variants.In addition,we assess the implications of the convergence of these pathways on the clinical employment of miRs as PCa biomarkers and therapeutic targets. | Ieva Eringyte Joanna N.Zamarbide Losada Sue M.Powell Charlotte L.Bevan Claire E.Fletcher | 2020 | Asian Journal of Urology2020，7，3： | 2 |
| 7 | Intradural spinal clear cell carcinoma causing cauda equina syndrome 显示文摘 | Gaetani P Di Ieva metastasis of renal A Colombo P | 2004 | Acta Neurochir (Wien)2004，146，8： | 1 |
| 8 | Cultural and Creative Industries concept --a historical perspective 显示文摘 | Ieva Moore | 2014 | Procedia - Social and Behavioral Sciences Volume 110 24 January 20142014，，： | 1 |
| 9 | Magnetic resonance elastog- raphy a general overview显示文摘 | Di Ieva A Grizzi F Rognone E | 2010 | Neurosurg Rev2010，33，： | 1 |
| 10 | Fraetal analysis of microvaseular networks in malignant brain tumors 显示文摘 | Di Ieva A | 2012 | Clin Neuropathol2012，31，5： | 1 |
| 11 | Simultaneous determination of oleuropein and hydroxytyrosol in rat plasma using liquid chromatography with fluorescence detection显示文摘 | Hai-Wei Tan Kellie L Tuck Ieva Stupans Peter J Hayball | 2002 | Journal of Chromatography B2002，，1： | 1 |
| 12 | Autoregulation of Helicobacter pylori Fur revealed by functional analysis of the iron-binding site显示文摘 | Delany I Spohn G Pacheco A B Ieva R Alaimo C Rappuoli R Scarlato V | 2002 | Molecular Microbiology2002，46，4： | 1 |
| 13 | An ovarian cancer malignancy risk index composed of HE4, CA125, ultrasonographic score, and menopausal status: use in differentiation of ovarian cancers and benign lesions显示文摘 | Ronalds Macuks Ieva Baidekalna Simona Donina | 2012 | Tumor Biology2012，，： | 1 |
| 14 | An ovarian cancer malignancy risk index composed of HE4, CA125, ultrasonographic score, and menopausal status: use in differentiation of ovarian cancers and benign lesions显示文摘 | Ronalds Macuks Ieva Baidekalna Simona Donina | 2012 | Tumor Biology2012，，5： | 1 |
| 15 | Effects of COVID-19 on the liver:The experience of a single center显示文摘BACKGROUND The coronavirus disease 2019(COVID-19)was perhaps the most severe global health crisis in living memory.Alongside respiratory symptoms,elevated liver enzymes,abnormal liver function,and even acute liver failure were reported in patients suffering from severe acute respiratory disease coronavirus 2 pneumonia.However,the precise triggers of these forms of liver damage and how they affect the course and outcomes of COVID-19 itself remain unclear.AIM To analyze the impact of liver enzyme abnormalities on the severity and outcomes of COVID-19 in hospitalized patients.METHODS In this study,684 depersonalized medical records from patients hospitalized with COVID-19 during the 2020-2021 period were analyzed.COVID-19 was diagnosed according to the guidelines of the National Institutes of Health(2021).Patients were assigned to two groups:those with elevated liver enzymes(Group 1:603 patients),where at least one out of four liver enzymes were elevated(following the norm of hospital laboratory tests:alanine aminotransferase(ALT)≥40,aspartate aminotransferase(AST)≥40,gamma-glutamyl transferase≥36,or alkaline phosphatase≥150)at any point of hospitalization,from admission to discharge;and the control group(Group 2:81 patients),with normal liver enzymes during hospitalization.COVID-19 severity was assessed according to the interim World Health Organization guidance(2022).Data on viral pneumonia complications,laboratory tests,and underlying diseases were also collected and analyzed.RESULTS In total,603(88.2%)patients produced abnormal liver test results.ALT and AST levels were elevated by a factor of less than 3 in 54.9%and 74.8%of cases with increased enzyme levels,respectively.Patients in Group 1 had almost double the chance of bacterial viral pneumonia complications[odds ratio(OR)=1.73,P=0.0217],required oxygen supply more often,and displayed higher biochemical inflammation indices than those in Group 2.No differences in other COVID-19 complications or underlying diseases were observed between groups.Preexisting hepatitis of a different etiology was rarely documented(in only 3.5%of patients),and had no impact on the severity of COVID-19.Only 5(0.73%)patients experienced acute liver failure,4 of whom died.Overall,the majority of the deceased patients(17 out of 20)had elevated liver enzymes,and most were male.All deceased patients had at least one underlying disease or combination thereof,and the deceased suffered significantly more often from heart diseases,hypertension,and urinary tract infections than those who made recoveries.Alongside male gender(OR=1.72,P=0.0161)and older age(OR=1.02,P=0.0234),diabetes(OR=3.22,P=0.0016)and hyperlipidemia(OR=2.67,P=0.0238),but not obesity,were confirmed as independent factors associated with more a severe COVID-19 infection in our cohort.CONCLUSION In our study,the presence of liver impairment allows us to predict a more severe inflammation with a higher risk of bacterial complication and worse outcomes of COVID-19.Therefore,patients with severe disease forms should have their liver tests monitored regularly and their results should be considered when selecting treatment to avoid further liver damage or even insufficiency. | Valentina Liakina Ieva Stundiene Gabriele Milaknyte Ramune Bytautiene Rosita Reivytyte Roma Puronaite Gintare Urbanoviciute Edita Kazenaite | 2022 | World Journal of Gastroenterology2022，28，39： | 1 |
| 16 | Disorders of neurocognitive function after coronary artery bypass grafting 显示文摘 | Ieva N | 2004 | Medicina (Kaunas)2004，40，1： | 1 |
| 17 | The microvascular network of the pituitary gland : a model for the application of fractal geometry to the analysis of angioarchiteeture and angiogenesis of brain tumors显示文摘 | Di Ieva A Grizzi E Ceva-Grimaldi G | 2010 | J Neurosurg Sci2010，54，2： | 1 |
| 18 | Fractal analysis of microvascular networks in malignant brain tumors显示文摘 | Di Ieva A | 2012 | Clin Neuropathol2012，31，5： | 1 |
| 19 | Gonadotropin-releasing hormone receptor expression in the human prostate显示文摘 | IEVA A STATHN P WIKSTROM P | 2001 | Prostate2001，47，： | 1 |
| 20 | Microvascular mor- phometrics of the hypophysis and pituitary tumors : From bench to operating theatre显示文摘 | Di Ieva A Weckman A Di Michele J | 2013 | Microvasc Res2013，89，： | 1 |
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