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| 1 | Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver显示文摘AIM To improve the Cultivation efficiency and yield of human liver cell line CL--l.METHODS High--density cultivation of CL--l onmicrocarriers was carried out with periodicobservation of their growth and proliferation.The specific functions of human liver cell werealso determined.RESULTS Cells of CL-1 cell line grew well onmicrocarrier Cytodex--3 and on the 7th day thepeak was reached. The amount of CLI cells was2.13 x 10’ and the total amount of albuminsynthesis reached 71 .23 ig, urea synthesis23 .32 mg and diazepam transformation 619 .7 ugrespectively. The yield of CL-l on microcarrierswas 49.3 times that of conventional cultivation.The amounts of albumin synthesis, ureasynthesis and diazepam transformation were39.8 times, 41 .6 times and 33.3 times those ofconventional Cultivation, respectively.CONCLUSION The human liver cell line CL--1 canbe Cultivated to a high density with Cytodex--3and has better biological functions. High-densityCultivation of CLI on microcarriers can act asthe biological material of bioartificial liver. | Gao Y Xu XP Hu HZ Yang JZ | 1999 | World Journal of Gastroenterology1999，5，3： | 13 |
| 2 | Kupffer cell and apoptosis in experimental HCC显示文摘INTRODUCTION Our previous study has proved that Kupffer cellsmay have an inhibitory effect on the process ofhepatocarcinogenesis,however,their inhibitorymechanism needs exploring deeply.We performed acomparative study on the expression of PCNA,Bax,P53 and apoptosis of liver cancer cells usingimmunohistochemical technology and terminaldeoxynucleotidyl transferase (TdT)-mediateddUTP-digoxigenin nick end labeling(TUNEL) | Zhu HZ Ruan YB Wu ZB Zhang CM | 2000 | World Journal of Gastroenterology2000，6，3： | 12 |
| 3 | Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates显示文摘AIM To develop a culture mode providingdurable biomaterials with high yields andactivities used in bioartificial liver.METHODS Hepatocytes were isolated from awhole pig liver by Seglen’s method of orthotopicperfusion with collagenase.In culture onmicrocarriers,primary porcine hepatocyteswere inoculated at a concentration of 5×10~7/mLinto the static culture systems containing 2 g/LCytodex-3,then supplemented with 100 mL/Lfetal calf serum(FCS)or 100 mL/L porcineportal vein serum(PPVS)respectively.Inspheroidal aggregate culture hepatocytes wereinoculated into 100 mL siliconized flasks at aconcentration of 5.0×10~6/mL.RESULTS In culture on microcarriershepatocytes tended to aggregate on Cytodex-3obviously after being inoculated.Typical multi-cellular aggregated spheroids could be found inthe two systems 24 h-48 h after hepatocyteswere cultured.The morphological charact-eristics and synthetic functions were maintainedfor 5 wk in FCS culture system and 8 wk in PPVSculture system.In spheroidal aggregate cultureabout 80%-90% isolated hepatocytes becameaggregated spheroids 24h after cultured insuspension and mean diameter of the spheroidswas 100μm.The relationship among thehepatocytes resembled that in the liver in vivo.Synthetic functions of albumin and urea of the spheroids were twice those of hepatocytescultured on monolayers.CONCLUSION As high-yields and high-activitymodes of culture on microcarriers or inspheroidal aggregate culture with portal veinserum are promising to provide biomaterials forbioartificial liver(BAL)efficiently. | Gao Y Hu HZ Chen K Yang JZ | 2000 | World Journal of Gastroenterology2000，6，3： | 6 |
| 4 | Non-invasive diagnosis of H pylori infection: Evaluation of serological tests with and without current infection marker CIM显示文摘AIM:To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of H pylori infection in adult dyspeptic patients. METHODS:Consecutive non-treated dyspeptic patients undergoing diagnostic endoscopy were tested for H pylori infection by culture, rapid urease test, and histology of gastric biopsy specimens. Serum from 61 H pylori infected and 21 non-infected patients were tested for anti-H pylori IgG antibodies by commercial ELISA (AccuBindTM ELISA, Monobind, USA), ICT (Assure H pylori Rapid Test, Genelabs Diagnostics, Singapore), and immunoblot (Helico Blot 2.1, Genelabs Diagnostics, Singapore) assays. ICT and immunoblot kits cover CIM among other parameters and their performance with and without CIM was evaluated separately. RESULTS:Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of ELISA were 96.7%, 42.8%, 83.1%, 81.8%, and 82.9%, of ICT were 90.1%, 80.9%, 93.2%, 73.9%, and 87.8%, of ICT with CIM were 88.5%, 90.4%, 96.4%, 73.0%, and 89.0%, of immunoblot were 98.3%, 80.9%, 93.7%, 94.4%, and 93.9%, and of immunoblot with CIM were 98.3%, 90.4%, 96.7%, 95.0%, and 96.3%, respectively. CONCLUSION:Immunoblot with CIM had the best performance. ICT with CIM was found to be more specific and accurate than the conventional ELISA and may be useful for non-invasive diagnosis of H pylori infection. | Sufi HZ Rahman M Golam Azam M Anisur Rahman MS Arfin M Mahbub Alam Tareq M Bhuiyan Nasim Ahmed Motiur Rahman Shamsun Nahar MS Hassan | 2008 | World Journal of Gastroenterology2008，14，8： | 4 |
| 5 | Experimental study of gold distribution in the algae-organic matter-fluid system显示文摘THE previous simulating experiments show that many algae can greatly accumulate goldfrom solution under some conditions. Some other experiments further indicate that organ-ic matter and crude oil can also play an important role in gold geochemistry processes such asleaching, migration, reduction and precipitation. However, these biometallogeneses are | Xie, SC Yin, HF Wang, HM Zhou, XG Fu, JM Sheng, GY Zhang, HZ Lu, JL | 1997 | Chinese Science Bulletin1997，42，19： | 3 |
| 6 | Expression and anti-apoptotic mechanism of Spodoptera littoralis Nucleopolyhedrovirus P49 protein显示文摘A novel cloned Spodoptera littoralis Nucleo-polyhedrovirus (SINPV) p49 gene is able to suppress apop-tosis of insect cells Sf9 triggered by virus. The amino acid sequence of P49 expressed in baculovirus expression system is the same as predicted, indicating that the expression of P49 is correct. Metabolic labeling revealed that p49 was able to be expressed both in the early and late phases after the viral infection, and only in the late phase was the expression driven by polyhedra promoter, but the amount of expression was higher than that of wtSlNPV. In summary, the early gene of SINPV p49 as well as p35 of AcMNPV is able to be expressed in the late phase, but its promoter is weaker compared with polyhedra promoter. In vitro, P49 can be cut by Bm caspase and human caspase-3, yielding 10 and 40 ku fragments. Purified P49 blocks the substrate cleavage by Bm caspase and human caspase-3, showing that P49 inhibits downstream caspases in the apoptotic pathway. | Pei, ZF Qi, YP Liu, YL Xiao, HZ | 2002 | Chinese Science Bulletin2002，47，8： | 2 |
| 7 | Cloning and identification of an angiostatic molecule IP-10/crg-2显示文摘AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and sequencing.CONCLUSION Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2. | Liu ZG Yang JH An HZ Wang HY He FT Han ZY Han Y Wu HP Xiao B Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 2 |
| 8 | Relative frequency and survival of primary cutaneous lymphomas: a retrospective analysis of 98 patients显示文摘 | Liu J Liu YH Jin HZ Ma DL Qu T Wang T S un QN Yu X | 2014 | Chinese Medical Journal2014，，4： | 2 |
| 9 | Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine显示文摘 | Tian HZ Yang BC Xu J | 2006 | J Sep Sci2006，29，17： | 1 |
| 10 | Zpalatoplasty (ZPP): a technique for patients without tonsils 显示文摘 | Friedman M lbrahim HZ Vidvasagar R Pomeranz J | 2004 | Otolaryngol Head Neck Surg2004，131，： | 1 |
| 11 | Transnssal endoscopic approach for juvenile nasopharyngeal angiofibroma显示文摘 | Chao WY | 1997 | Am J Otolaryngol1997，18，2： | 1 |
| 12 | Prevalence of vitamin D deficiency rickets in adolescent school girls in Western region,Saudi Arabia显示文摘 | Siddiqui AM Kamfar HZ | 2007 | Saudi Med J2007，28，： | 1 |
| 13 | Diagnostic accuracy of adenosine deaminase in tuberculous pleurisy:a meta-analysis显示文摘 | Liang QL Shi HZ Wang K | 2008 | Respir Med2008，102，5： | 1 |
| 14 | Carotid plaque c- omposition and cerebral infarction: MR imaging study 显示文摘 | Ouhlous M Flach HZ De Weert TT | 2005 | AJNR Am J Neuroradiol2005，26，5： | 1 |
| 15 | Effects and mechanisms of aloe-emodin on cell death in human lung squamous cell carcino -ma显示文摘 | Lee HZ Hsu SL Liu MC | 2001 | Eur J Pharmacology2001，431，3： | 1 |
| 16 | Effect of iron or multiple micronutrient supplements on the prevalence of anaemia among anaemic young children of a malariaendemic area: a randomized double-blind trial显示文摘 | Ouedraogo HZ Dramaix-Wilmet M Zeba AN etal | 2008 | Trop Med Int Health2008，13，10： | 1 |
| 17 | The oxidative stress status in diabetes mellitus and diabetic nephropathy显示文摘 | Pan HZ Zhang L Guo MY | 2010 | Acta Diabetol2010，47，1： | 1 |
| 18 | Metformin reverses fatty liver disease in obese,leptin-deficient mice显示文摘 | Lin HZ Yang SQ Chuckaree C | 2000 | Nat Med2000，6，： | 1 |
| 19 | Prediction of effective thermalconductivities of woven fabric composites using unit cells atmultiple length scales 显示文摘 | Li HZ Li S G Wang Y C | 2011 | Journal of Materials Research2011，26，： | 1 |
| 20 | Effect of mifepristone on the expression of cytokines in the human Fallopian tube 显示文摘 | Li HZ Sun X Stavreus-Evers A | 2004 | Mol Hum Reprod2004，10，： | 1 |
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