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| 1 | Effects of Flower Buds Extract of Tussilago farfara on Focal Cerebral Ischemia in Rats and Inflammatory Response in BV2 Microglia显示文摘Objective: To investigate the effects of the flower buds extract of Tussilago farfara Linné(Farfarae Flos; FF) on focal cerebral ischemia through regulation of inflammatory responses in activated microglia. Methods: Brain ischemia was induced in Sprague-Dawley rats by a transient middle cerebral artery occlusion(t MCAO) for 90 min and reperfusion for 24 h. Twenty rats were randomly divided into 4 groups(n=5 per group): normal, t MCAO-induced ischemic control, t MCAO plus FF extract 300 mg/kg-treated, and t MCAO plus MK-801 1 mg/kg-treated as reference drug. FF extract(300 mg/kg, p.o.) or MK-801(1 mg/kg, i.p.) was administered after reperfusion. Brain infarction was measured by 2,3,5,-triphenyltetrazolium chloride staining. Neuronal damage was observed by haematoxylin eosin, Nissl staining and immunohistochemistry using anti-neuronal nuclei(Neu N), anti-glial fibril ary acidic protein(GFAP), and antiCD11 b/c(OX42) antibodies in ischemic brain. The expressions of inducible nitric oxide synthase(i NOS), tumor necrosis factor(TNF-α), and hypoxia-inducible factor-1 a(HIF-1α) were determined by Western blot. BV2 microglial cel s were treated with FF extract or its main bioactive compound, tussilagone with or without lipopolysaccharide(LPS). Nitric oxide(NO) production was measured in culture medium by Griess assay. The expressions of i NOS, COX-2 and pro-inflammatory cytokines mRNA were analyzed by reverse transcription-polymerase chain reaction. The expression of i NOS, and COX-2 proteins, the phosphorylation of ERK1/2, JNK, and p38 MAPK and the nuclear expression of NF-κB p65 in BV2 cells were determined by Western blot. Results: FF extract significantly decreased brain infarctions in ischemic rats(P<0.01). The neuronal death and the microglia/astrocytes activation in ischemic brains were inhibited by FF extract. FF extract also suppressed i NOS, TNF-α, and HIF-1α expression in ischemic brains. FF extract(0.2 and 0.5 mg/m L, P<0.01) and tussilagone 20 and 50 μmol/L, P<0.01) significantly decreased LPS-induced NO production in BV2 microglia through downregulation of i NOS m RNA and protein expression. FF extract and tussilagone significantly inhibited LPS-induced expression of TNF-α, IL-1β, and IL-6 m RNA, and also suppressed the phosphorylation of ERK1/2, JNK and p38 MAPK and the nuclear expression of NF-κB in a dosedependent manner. Conclusion: FF extract has a neuroprotective effect in ischemic stroke by the decrease of brain infarction, and the inhibition of neuronal death and microglial activation-mediated inflammatory responses. | Ji Hye Hwang Vinoth Kumar R Seok Yong Kang Hyo Won Jung Yong-Ki Park | 2018 | Chinese Journal of Integrative Medicine2018，24，11： | 13 |
| 2 | Effect of blood cyclosporine concentration on the outcome of hematopoietic stem cell transplantation from an HLA-matched sibling donor显示文摘 | Kanda Y Hyo R Yamashita T | 2006 | Am J Hematol2006，81，11： | 1 |
| 3 | Current status and per- spectives of biopharmaceutical drugs 显示文摘 | JAE K R HYO S K DOO H N | 2012 | Biotechnology and Bioprocess Engineering2012，17，： | 1 |
| 4 | Particle deposition of therapeutic aerosols in the nose and paranasal sinuses after transnasal sinus surgery:A cast model study显示文摘 | SAIJO R MAJIMA Y HYO N | 2004 | Am J Rhinol2004，18，： | 1 |
| 5 | Diffuse large B cell lymphoma without immunoglobulin light chain restriction by flow cytometry显示文摘 | Tomita N Takeuchi K Hyo R | 2009 | Acta Haematol2009，121，4： | 1 |
| 6 | Particle deposition of therapeutic aerosols in the nose and paranasal sinuses after transnasal sinus surgery : a cast model study 显示文摘 | Saijo R Majima Y Hyo N | 2004 | Am J Rhinol2004，18，1： | 1 |
| 7 | Four-color flow cytometric analysis of myeloma plasma cells显示文摘 | Kobayashi S Hyo R Amitani Y | | 0，，06： | 1 |
| 8 | Four-color flow cytometric analysis of myeloma plasma cells显示文摘 | Kobayashi S Hyo R Amitani Y | 2006 | Am J Clin Pathol2006，126，： | 1 |
| 9 | Diffuse large B cell lymphoma without immunoglobulin light chain restriction by flow cytometry显示文摘 | Tomita N Takeuchi K Hyo R | | 0，，04： | 1 |
| 10 | Four-color flow cytometricanalysis of myeloma plasma cells 显示文摘 | Kobayashi S Hyo R Amitani Y | 2006 | Am J Clin Pathol2006，126，6： | 1 |
| 11 | Mechanical behavior and failure process during compressive and shear deformation of honeycomb composite at elevated temperatures 显示文摘 | Hyo S Lee Soon H Hong Jae R Lee | 2002 | J Mater Sci2002，37，： | 1 |
| 12 | Comparison of autologous and allogeneic hematopoietic stem cell transplantation for extranodal NK/T cell lymphoma,nasal-type:analysis of The Japan Society for Hematopoietic Cell Transplantation (JSHCT) Lymphoma Working Group显示文摘 | Suzuki R Kako S Hyo R | 2011 | Blood2011，118，50： | 1 |
| 13 | Diffuse large B cell lymphoma without immunoglobulin light chain restriction by flow cytometry显示文摘 | Tomita N Takeuchi K Hyo R | 2009 | Acta Haematol2009，121，4： | 1 |
| 14 | Physical Modeling of Gas/Liquid Mass Transfer in a Gas Stirred Ladle显示文摘 | Seon Hyo Kim Fruehan R J | 1987 | Metallurgical Transactions B1987，18，12： | 1 |
| 15 | The Therapeutic Effect of Rituximab on CDS-positive and CDS-negative Diffuse Large B- cell Lymphoma显示文摘 | Hyo R Tomita N Takeuchi K | 2009 | Hematol Oncol2009，，： | 1 |
| 16 | Improved prognosis of NK-cell lymphoma/leukemia,but not for T-cell lymphomas:a nationwide survey of NK-and associated T-cell neoplasms by the NK-cell Tumor Study Group (NKTSG)显示文摘 | Hyo R Ishida F Isobe Y | 2011 | Ann Oncol2011，22，4： | 1 |
| 17 | Four color flow cytometric analysis of myeloma plasma cells显示文摘 | KOBAYASHI S HYO R AMITANI Y | 2006 | Am J Clin Pathol2006，126，： | 1 |
| 18 | The effect off V2O5 on the sintsbility and physical properties of BiO3-NiO-Nb2O5 and BiO3-ZnO-Nb2O5 temperaturestable dielectrics显示文摘 | KYEONG A Y KYOUNG R H HYO T K | 1999 | Journal off Materials Science1999，34，： | 1 |
| 19 | Simultaneous expression of cyclooxygenase-2 and microsomal prostaglandin E synthase in squamous cell carcinoma of the larynx显示文摘 | Kawata R Hyo S Maeda T | 2006 | Acta OtoLaryngol2006，126，6： | 1 |
| 20 | Thetherapeutic effect of rituximab on CD5-positive andCD5-negative diffuse large B-cell lymphoma显示文摘 | HYO R TOMITA N TAKEUCHI K | 2010 | He-matol Oncol2010，28，： | 1 |
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