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| 1 | Derivation and characterization of human embryonic stem cell lines from poor quality embryos显示文摘Poor quality embryos discarded from in vitro fertilization (IVF) laboratories are good sources for deriving human embryonic stem cell (hESC) lines. In this study, 166 poor quality embryos donated from IVF centers on day 3 were cultured in a blastocyst medium for 2 days, and 32 early blastocysts were further cultured in a blastocyst optimum culture medium for additional 2 days so that the inner cell masses (ICMs) could be identified and isolated easily. The ICMs of 17 blastocysts were isolated by a mechanical method, while those of the other 15 blastocysts were isolated by immunosurgery. All isolated ICMs were inoculated onto a feeder layer for subcultivation. The rates of ICM at- tachment, primary ICM colony formation and the efficiency of hESC derivation were similar between the ICMs isolated by the two methods (P>0.05). As a result, four new hESC lines were established. Three cell lines had normal karyotypes and one had an unbalanced Robertsonian translocation. All cell lines showed normal hESC characteristics and had the differentiation ability. In conclusion, we established a stable and effective method for hESC isolation and culture, and it was confirmed that the mechanical isolation was an effective method to isolate ICMs from poor embryos. These results further indicate that hESC lines can be derived from poor quality embryos discarded by IVF laboratories. | Weiqiang Liu, Yifei Yin, Xiaolin Long, Yumei Luo, Yonghua Jiang, Wenhong Zhang, Hongzi Du, Shaoying Li, Yuhong Zheng, Qing Li, Xinjie Chen, Baoping Liao, Guohong Xiao, Weihua Wang, Xiaofang Sun Guangzhou Key Laboratory of Reproductive and Genetics Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, China | 2009 | Journal of Genetics and Genomics2009，36，4： | 3 |
| 2 | Impact of traffic influxes:Revealing exponential intercontact time in urban VANETs显示文摘 | ZHU Hongzi Li Minglu FU Luoyi | | 0，，8： | 1 |
| 3 | Efficient meth- ods for choosing quantum channel in teleportation and bidirectional teleportation显示文摘 | HU Yang FU Hongzi TIAN Xiulao | 2013 | Journal of China Uni- versities of Posts and Telecommunications2013，20，2： | 1 |
| 4 | A General Meth- od of Selectiong Quantum Channel for Bidirectional Quantum teleportation 显示文摘 | FU Hongzi Tian Xiulao Hu Yang | 2014 | International Jourmal of Theoretical Physics2014，53，6： | 1 |
| 5 | HERO:online real-time vehicle tracking显示文摘 | Zhu Hongzi Li Minglu Zhu Yanmin | 2009 | IEEE Trans on Parallel and Distributed Systems2009，20，5： | 1 |
| 6 | HERO: On- line real-time vehicle tracking 显示文摘 | Zhu Hongzi Li Minglu Zhu Yanmin | 2009 | IEEE Transactions on Parallel and Distributed Systems2009，20，5： | 1 |
| 7 | HERO: On- line real-time vehicle tracking 显示文摘 | Zhu Hongzi Li Minglu Zhu Yanmin | 2009 | IEEE Transactions on Parallel and Distributed Systems2009，20，5： | 1 |
| 8 | A compressive sensing approach to urban traffic estimation with probe vehicles 显示文摘 | Zhu Yanmin Li Zhi Zhu Hongzi | 2013 | IEEE Transactions on Mobile Computing2013，12，11： | 1 |
| 9 | Impact of traffic influxes: Revealing exponential intercontact time in urban VANETs显示文摘 | Zhu Hongzi Li Minglu Fu Luoyi | 2011 | IEEE Transactions on Parallel and Distribu- ted Systems2011，22，8： | 1 |
| 10 | Application and research pro- gress of liquid-phase microextraction in pharmaceutical analysis[J]显示文摘 | MAWT LEJ HONGZY etal | | 中国药学杂志0，45，6： | 1 |
| 11 | Positive quasi-periodic solutions to Lotka-Volterra system显示文摘In this paper,we prove the existence of positive quasi-periodic solutions for a class of Lotka-Volterra system with quasi-periodic coefficients by KAM technique. | Cong HongZi Mi LuFang Yuan XiaoPing | 2010 | Science China Mathematics2010，53，5： | 1 |
| 12 | Effects of baffles on separation of aqueous ethanol solution with hollow fibers显示文摘The effects of baffles on the operation and mass-transfer characteristics in novel hollowfiber membrane contactor used in distillation were investi-gated.Hollowfiber membranes,coated with a 7μm polydimethyl-siloxane,were operated as structural packing in the separation of ethanol-water solutions.The parallelflow mode was chosen for separation due to the stronger driving force of the concentration difference,in which liquidflows through the lumens of thefibers and vaporflows countercurrent-wise outside thefibers.Two baffles were installed on the shell side of the membrane contactors to enhance separation,which had a round shape with a semi-lunar hole.The results show that both baffled and unbaffled membrane contactors gave better,more produc-tive separations than traditional packing in distillation,such as the excellent Sulzer Gauze BX structured packing.The baffled membrane contactors performed better than unbaffled ones,especially at high vapor velocities.The minimal HTU of membrane contactor with baffles could reach as low as 4.5 cm,and almost all the contactors could work well above the limit whereflooding normally occurs in conventional cases.Theoretical analysis predicted that baffles helped membrane module to obtain a higher mass-transfer coefficient and a smaller mass-transfer resistance.Finally,theoretical mass-transfer coefficient and experi-mental value were compared as well as the contribution of each individual mass-transfer coefficients among liquid,gas and membrane. | Zhihong YANG Guoliang ZHANG Lan LIN Danping REN Qin MENG Hongzi ZHANG | 2009 | Frontiers of Chemical Science and Engineering2009，3，1： | 0 |
| 13 | Study of stereoselective pharmacokinetics of anisodamine enantiomers in rabbits by capillary electrophoresis显示文摘The purpose of this study was to determine the pharmacokinetics of anisodamine enantiomers in plasma after oral and intravenous administration of racemic anisodamine in rabbits. A capillary electrophoresis method for the simultaneous separation of two pairs ofenantiomers in plasma has been firstly developed and validated. Usinga 75 mM phosphate buffer containing 25 mM carhoxymethylated-gamma cyclodextrin at pH 2.5, | FanGR HongZY LinM YinXP WuYT | 2005 | 第二军医大学学报2005，26，3： | 0 |
| 14 | Vehicular Communications,Networks,and Applications显示文摘A vehicular ad hoc network(VANET)is a packet-switched network,consisting of mobile communication nodes mounted on vehicles,with very limited or no infrastructure support[1].It supports communications among nearby vehicles,and between vehicles and nearby infrastructure/users,including vehicle-to-vehicle(V2V)。 | ZHUANG Weihua ZHU Hongzi | 2016 | ZTE Communications2016，14，3： | 0 |
| 15 | Real-time urban traffic information estimation with a limited number of surveillance cameras显示文摘经常的交通拥挤消费精力的庞大的数量并且引起极大地增加的旅行时间。因此,因为如此的信息对更有效的交通控制并且旅行计划无价,即时交通信息对公众很重要。然而,在象上海一样的宗主国获得如此的信息由于内在的道路网络的非常地大的规模和复杂性是很挑战性的。在这份报纸,我们建议利用弥漫地在城市里部署的监视照相机的一个新奇交通评价计划。与仅仅有照相机的一小部分道路,我们采用推断交通的评价方法没有照相机在那些道路上调节的一个 measurementbased 交通矩阵(TM ) 。广泛地踪迹驱动的模拟以及地学习结果证明我们的计划能与大小的一个很有限的数字完成高精确性。我们的基于测量的算法的精确性在多达 50% 超过传统的基于速度、基于模型的途径。 | Guangtao XUE Ke ZHANG Qi HE Hongzi ZHU | 2012 | Frontiers of Computer Science2012，6，5： | 0 |
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