|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Optimization of Extraction of Total Flavonoids from HERBA BLUMEAE RIPARIAE Using Response Surface Methodology显示文摘[Objectives]This study aimed to optimize the ultrasonic-assisted extraction process of total flavonoids from a Zhuang medicine HERBA BLUMEAE RIPARIAE using response surface methodology.[Methods]Using rutin as a reference substance,a standard curve was drawn at a wavelength of 292 nm.Content determination was performed through absorbance under different conditions.The effects of ultrasonic time,ethanol volume fraction and liquid-to-solid ratio on the yield of total flavonoids from HERBA BLUMEAE RIPARIAE were investigated by single-factor tests.By using Box-Behnken test,the ultrasonic-assisted extraction process of total flavonoids from HERBA BLUMEAE RIPARIAE was optimized.[Results]The optimal extraction process of total flavonoids from HERBA BLUMEAE RIPARIAE was as follows:ultrasonic time of 30 min,ethanol volume fraction of 50%and liquid-to-solid ratio of 40∶1(mL/g).Under the optimal extraction conditions,the average yield of total flavonoids from HERBA BLUMEAE RIPARIAE was 85.47 mg/g.[Conclusions]The optimized extraction process was convenient and feasible.This study will provide a reference for the extraction of total flavonoids from HERBA BLUMEAE RIPARIAE,and provide certain guidance and basis for further development and utilization of the plan resource of Blumea riparia(Bl.)DC. | Hongna SU Yining ZHANG Xuexue LI Xuelian ZHAO Yisong LI Wenbing LI Yuan LIU | 2020 | Medicinal Plant2020，11，2： | 5 |
| 2 | Electromyography and Fos immunostaining study establish a possible functional link between trigeminal proprioception and the oculomotor system in rats显示文摘The objective of this study was to explore whether there was a functional link between trigeminal proprioception and the oculomotor system mediated through jaw muscle afferents.Electromyography(EMG) was undertaken of the levator palpebrae(LP) and superior rectus(SR),and Fos expression was detected in the brainstem following consecutive down-stretching of the lower jaw at 2-4 Hz in rats.Retrograde tracing was undertaken of the interstitial nucleus of Cajal and Darkschewitsch nucleus(INC/DN) pre-oculomotor neurons.FMG-like responses were recorded from the LP/SR during down-stretching of the lower jaw at 2-4 Hz in 3 out of 11 rats.Fos expression was induced by consecutive down-stretching of the lower jaw at 2-4 Hz for 20-30 seconds.Interestingly,Fos expression was distributed mainly in the bilateral INC/DN area.We also examined Fos-like immunoreactivity in central mesencephalic and paramedian pontine reticular formation that harbors premotor neurons controlling horizontal eye movement,but no Fos-like staining was observed therein.By injection of retrograde tracers into the oculomotor nucleus combined with Fos immunostaining,double labeled pre-oculomotor neurons were visualized to distribute in the INC/DN.In conclusions,there may exist a trigeminal proprioceptive-oculomotor system neural circuit through jaw muscle afferents in rats.Judging from Fos distribution pattern,this pathway might be related to vertical and torsional eye movements. | Houcheng Liang Jingdong Zhang Pifu Luo Hongna Zhu Ying Qiao Anle Su Ting Zhang | 2017 | The Journal of Biomedical Research2017，31，3： | 2 |
| 3 | Optimization of Ultrasonic-enzymatic Extraction of Total Flavonoids from Cardamine tangutorum O.E.Schulz by Response Surface Methodology and Content Determination显示文摘[Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of total flavonoids in C.tangutorum O.E.Schulz was optimized by Box-Behnken design from aspects of ethanol volume fraction,extraction time,extraction temperature and enzyme addition,with yield of total flavonoids as the evaluation index.[Results]The optimal extraction process of total flavonoids in C.tangutorum O.E.Schulz was as follows:ethanol volume fraction of 70%,ultrasonic extraction time of 43 min,ultrasonic extraction temperature of 37℃and cellulose addition of 0.07 mg.Under the optimal conditions,the yield of total flavonoids from C.tangutorum O.E.Schulz reached 75.90 mg/g.[Conclusions]The optimized extraction process was reasonable and feasible,and it could provide a reference for the extraction of total flavonoids in C.tangutorum O.E.Schulz.This study provided a foundation for the quality control of food/medicinal materials and the development and research of big health products of C.tangutorum O.E.Schulz. | Xiaoxia CAI Bochao LI Xuexue LI Hongna SU Wenbing LI Yuan LIU | 2020 | Medicinal Plant2020，11，6： | 2 |
| 4 | Study on the Forming Process of Yi Medicine Tongfeng Granules显示文摘[Objectives]To optimize the forming process of Yi medicine Tongfeng Granules.[Methods]The forming process of Yi medicine Tongfeng Granules was optimized,with paste density,ethanol volume fraction,and type and proportion of excipient as influencing factors,and granule formability,solubility,moisture absorption,and angle of repose as evaluation indicators.Critical relative humidity(CRH)was investigated to select optimal storage conditions.[Results]Maltodextrin was selected as the excipient,and the best process parameters was the ratio of drug to excipient at 1∶2(g/g),under which the forming rate,solubility,moisture absorption rate,and angle of repose were 81.38%,98.90%,8.81%,and 27.5°,respectively.The critical relative humidity was 72%.[Conclusions]The forming process adopted is reasonable and feasible,and can provide a reference for large-scale production of Yi medicine Tongfeng Granules. | Xuelian ZHAO Yisong LI Ling TAN Hongna SU Junzhang QUBIE Wenbing LI Yuan LIU | 2021 | Medicinal Plant2021，12，6： | 0 |
| 5 | Chemical profiling and rapid discrimination of Blumea riparia and Blumea megacephala by UPLC-Q-Exactive-MS/MS and HPLC显示文摘Objective:To rapidly identify the two morphologies and chemical properties of similar herbal medicines,Blumea riparia and B.megacephala as the basis for chemical constituent analysis.Methods:UPLC-Q-Exactive-MS/MS was utilized for profiling and identification of the constituents in B.riparia and B.megacephala.Chemical pattern recognition(CPR)was further used to compare and distinguish the two herbs and to identify their potential characteristic markers.Then,an HPLC method was established for quality evaluation.Results:A total of 93 constituents are identified,including 54 phenolic acids,35 flavonoids,two saccharides,one phenolic acid glycoside,and one other constituent,of which 67 were identified in B.riparia and B.megacephala for the first time.CPR indicates that B.riparia and B.megacephala samples can be distinguished from each other based on the LC–MS data.The isochlorogenic acid A to cryptochlorogenic acid peak area ratio calculated from the HPLC chromatograms was proposed as a differentiation index for distinguishing and quality control of B.riparia and B.megacephala.Conclusion:This study demonstrates significant differences between B.riparia and B.megacephala in terms of chemical composition.The results provide a rapid and simple strategy for the comparison and evaluation of the quality of B.riparia and B.megacephala. | Hongna Su Xuexue Li Ying Li Yuanlin Kong Jianlong Lan Yanfei Huang Yuan Liu | 2023 | Chinese Herbal Medicines2023，15，2： | 0 |
| 6 | Quality Standard of Zijinbiao显示文摘[Objectives]To establish the quality standard for Zijinbiao.[Methods]Microscopic identification,physical and chemical identification,and thin-layer chromatography(TLC)were used to qualitatively identify Zijinbiao.The moisture,total ash,acid-insoluble ash,and alcohol-soluble extract content were determined.The content of Plumbagin was determined by high performance liquid chromatography(HPLC).[Results]Microscopic identification,physical and chemical identification and thin layer identification features were remarkable.The moisture,total ash,acid-insoluble ash,and extract content of the 15 batches of samples were 7.49%-11.84%,2.43%-5.81%,0.59%-3.18%and 13.80%-20.45%,respectively.The linear equation of Plumbagin was Y=38094X,R^(2)=0.9996.Plumbagin had a good linear relationship in the range of 0.01-0.53 mg/mL.[Conclusions]This method is specific and reproducible,and can be used for quality control of Zijinbiao. | Jianlong LAN Yuebu HAILAI Cijia DIJIU Hongna SU Li LI Zhengming YANG Yuan LIU | 2022 | Medicinal Plant2022，13，5： | 0 |
| 7 | Identification of Tibetan Medical Material Dracocephalum tanguticum Maxim.显示文摘[Objectives]To explore morphological identification,macroscopical identification,microscopic identification,thin layer chromatography(TLC)identification of Tibetan medical material Dracocephalum tanguticum Maxim.,and provide experimental data for its identification and application.[Methods]The Tibetan medical material was identified by means of original plant,characters,powder,paraffin section and thin layer chromatography(TLC).[Results]Tibetan medical material D.tanguticum Maxim.was obviously distinguished in character identification and microscopic identification,and the TLC method was simple and feasible.[Conclusions]The results will provide the source work foundation for the formulation of the quality standard of Sichuan Province(draft)for Tibetan medicinal material'D.tanguticum Maxim.'and the development of pharmaceutical preparations for medical institutions. | Ling TAN Pei QUN Hongna SU Xuelian ZHAO Yisong LI Yanfei HUANG Yuan LIU | 2021 | Medicinal Plant2021，12，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员