|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | X-ray measurement of laser fusion targets using least squares fitting显示文摘 | Singleton R M Weinstein B W Hendricks C D | 1979 | Appl Opt1979，18，24： | 2 |
| 3 | A mechanism for zinc toxicity in neuroblastoma cells显示文摘 | Daniels W M U Hendricks J Salie R | 2004 | Metabolic Brain Disease2004，19，12： | 1 |
| 4 | Consistency,microstructure and probiotic survivability of goats’milk yoghurt using polymerized whey protein as a co-thickening agent显示文摘 | WANG W B BAO Y H HENDRICKS G M | 2012 | Int Dairy J2012，24，2： | 1 |
| 5 | Absolute Integrated SAXS Measurements During the Crystallization of Linear Polyethylene 显示文摘 | Lin J S Hendricks R W Schuhz J M | 1982 | J Polym Sci: Polym Phy Ed1982，20，8： | 1 |
| 6 | Feedback Control of Von Karman Vortex Shedding Behind a Circular Cylinder at Low Reynolds Numbers显示文摘 | Park D S Ladd D M Hendricks E W | 1994 | Physics of Fluids1994，6，7： | 1 |
| 7 | Homing of hatchery-reared American shad to the Lehigh River, a tributary to the Delaware River) 显示文摘 | Hendricks M L Hoopes R 1 Arnold D A | 2002 | North American Journal of Fisheries Management2002，22，： | 1 |
| 8 | Effects of transglutaminase treatment on functional properties and probiotic culture survivability of goat mild yogurt显示文摘 | FARNSWORTH J P LI J HENDRICKS G M | 2006 | Small Ruminant Research2006，65，1： | 1 |
| 9 | Clinical tests to di- agnose lumbar segmental instability: a systematic review显示文摘 | Alqarni A M Schneiders A G Hendrick P A | 2011 | J Orthop Sports Phys Ther2011，41，3： | 1 |
| 10 | Protective effect of dietary tomatine against dibenzopyrene (OBP)-induced liver and stomach tumors in rainbow trout 显示文摘 | Friedman M McQuistan T Hendricks J D | 2007 | Molecular Nutrition and Food Research2007，51，12： | 1 |
| 11 | Column Web Compression Strength at End-Plate Connections显示文摘 | A Hendrick & T M Murray | 1984 | Engineer Journal1984，，3： | 1 |
| 12 | 18β-glycyrrhetinic acid inhibits rotavirus replication in culture显示文摘 | Hardy M E Hendricks J M Paulson J M | 2012 | Virology2012，，9： | 1 |
| 13 | Effect of heat stress on production parameters and immune responses of commer- cial laying hens显示文摘 | Mashaly M M Hendricks G L Kalama M A | 2004 | Poultry Science2004，83，： | 1 |
| 14 | Active stabiliza tion of rotating stall in a three-stage axial compressor显示文摘 | Haynes J M Hendricks G J Epstein A H | 1994 | Journal of Turbomachinery1994，116，： | 1 |
| 15 | Antidepressant medications, mood and male fertility 显示文摘 | Hendrick V Giflin M Ahshuler L | 2000 | Psychoneuroendocrinology2000，25，1： | 1 |
| 16 | Electrocardiographic changesassociated with ritodrine induced matemaltachycardia and hypokal-aemia显示文摘 | Hendricks SK Keroes J Katz M | 1986 | Am J Obstet Gynecol1986，154，4： | 1 |
| 17 | Abnormal Fhit expression in malignant and premalignant lesions of the cervix 显示文摘 | Birrer M J Hendricks D Farley J | 1999 | Cancer Res1999，59，20： | 1 |
| 18 | A small perturbation CFD method for calculation of seal rotordynamic coefficients 显示文摘 | Athavale M M Hendricks R C | 1996 | International Journal of Rotating Machinery1996，2，3： | 1 |
| 19 | Active stabilization of rotating stall in a three stage axial compressor 显示文摘 | Haynes J M Hendricks G J Epstein A H | 1994 | Journal of Turbomachinery1994，116，2： | 1 |
| 20 | A Drosophila model for age-associated changes in sleep,wake cycles显示文摘 | Koh K Evans J M Hendricks J C | 2006 | Proe Natl Acad Sci USA2006，103，13： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员