|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 日本血吸虫中国大陆株TPI DNA疫苗诱导BALB/c小鼠保护性免疫力的研究显示文摘目的 研究日本血吸虫中国大陆株磷酸丙糖异构酶 (Sj CTPI) DNA疫苗诱导 BAL B/ c小鼠的保护性免疫作用。方法 将 39只 BAL B/ c小鼠随机分成 3组 :A组 (对照组 ) ,肌注 pc DNA3.1质粒 DNA10 0 μg/鼠 ;B组 (实验组 ) ,每鼠肌注 pc DNA3.1- Sj CTPI10 0 μg;C组 (加强组 ) ,每鼠肌注 pc DNA3.1- Sj CTPI10 0 μg及 pc DNA3.1- P35和 pc DNA3.1-P40的混合物 10 0 μg。每两周免疫 1次 ,共计 3次。末次免疫 4周后 ,每鼠用 45条尾蚴进行攻击 ,45 d后剖杀小鼠 ,计数成虫以及每鼠肝组织内虫卵数 ;于攻击前每组各取两只小鼠采用 51 Cr释放法测定 Sj CTPI介导的特异性细胞毒作用 ;EL ISA检测攻击前后的抗 TPI的抗体水平。 结果 用 EL ISA检测抗 TPI抗体的结果表明 ,攻击前 A组的 10份血清均为阴性 ,B组 5 / 10份血清出现弱阳性 ,C组也有 6 / 10份血清出现弱阳性反应。51 Cr释放法测定细胞毒活性结果表明 ,A、B、C组细胞毒活性分别为 9.1%、2 7.6 %和 5 4.4%。与对照组相比 ,实验组的减虫率、减卵率分别为 30 .2 %、5 2 .9% ;加强组的减虫率、减卵率分别为 32 .7%、47.0 %。 结论 进一步证实了 Sj CTPI DNA疫苗作为抗血吸虫病核酸疫苗的潜力。 | 司进 朱荫昌 Harn DA 殷旭仁 余传信 何伟 任建功 华万全 | 2001 | 中国寄生虫病防治杂志2001，14，3： | 20 |
| 2 | 日本血吸虫SjCTPI-Hsp70 DNA疫苗与白细胞介素12对水牛的联合免疫保护作用研究显示文摘目的探讨中国大陆株日本血吸虫磷酸丙糖异构酶-热激蛋白(SjCTPI-Hsp70)DNA疫苗联合佐剂白细胞介素-12(IL-12)质粒DNA对水牛的免疫保护作用。方法实验采用双盲法,所用疫苗及制剂均在实验结束后解码。将购自非血吸虫病流行区45头8~10月龄健康水牛随机分为A组(SjCTPI-Hsp70+IL-12,300μg)、B组(SjCTPI+IL-12,300μg)和C组(空质粒pVAX+IL-12,300μg)等3组(每组15头),每头牛分别经肩部肌肉注射免疫3次,每次间隔28d。末次免疫后28d,每头牛经大腿内侧皮肤感染日本血吸虫尾蚴1000条。解剖前2d及当天分别收集粪便1次,用定量法计数虫卵和毛蚴。攻击感染后56d解剖,用生理盐水经胸主动脉灌冲法收集、计数成虫,检测每克肝组织虫卵数。结果A、B组减虫率分别为51.2%和41.5%(χ2=1.89,P>0.05),减雌虫率分别为48.9%和44.7%(χ2=0.35,P>0.05),减粪卵率分别为52.1%和38.3%(χ2=3.84,P<0.05),减毛蚴率为52.1%和33.2%(χ2=7.30,P<0.01)及减肝卵率为61.5%和42.0%(χ2=7.61,P<0.01)。结论用SjCTPI-Hsp70+IL-12免疫水牛可获得一定的免疫保护性作用。 | 喻鑫玲 何永康 熊铁 赵雅琴 石孟芝 周杰 刘宗传 罗新松 付晓 贺宏斌 DA HARN 李岳生 | 2006 | 中国寄生虫学与寄生虫病杂志2006，24，6： | 3 |
| 3 | T and B cell epitope mapping of SM23,an integral membrane protein of Schistosoma mansoni显示文摘 | Reynolds SR Shoemaker CB Harn DA | 1992 | J Immunol1992，149，12： | 1 |
| 4 | Evidence that a protective membrane epitope is involved in early but not late phase immunity in Schistosoma mansoni显示文摘 | Harn DA Quinn JJ Cianci CM | 1985 | J Immunol1985，135，3： | 1 |
| 5 | Suppression of cathepsin B expression in Schistosomamansoni by RNA interference显示文摘 | Skelly PJ Da'dara A Harn DA | 2003 | Int J Parasitol2003，33，4： | 1 |
| 6 | Comparison of Irradiated-cercaria Schistosome Vaccine Models That Use 15-and 50-kilorad Doses:the 15-kilorad Dose Gives Greater Protection,Smaller Liver Sizes,and Higher Gamma Interferon Levels after Challenge显示文摘 | Reynolds SR Harn DA | 1992 | Infect Immun1992，60，： | 1 |
| 7 | Protective immunity induced with 23000 membrane protein DNA vaccine of Schistosoma japonicum Chinese strain in infected C57BL/6 mice显示文摘 | Zhu Y Ren J Harn DA | 2003 | J Trop Med Pub Health2003，34，4： | 1 |
| 8 | Isotype responses to candidate vaccine antigens in protective sera obtained from mice vaccinated with irradiated cercariae of Schistosoma mansoni显示文摘 | Rithter D Incani RN Harn DA | 1993 | Infect Immun1993，61，： | 1 |
| 9 | Comparison of irradiated-cercaria schistosome vaccine models that use 15-and 50-kilorad doses:the 15-kilorad dose gives greater protection,smaller liver sizes,and higher gamma interferon levels after challenge显示文摘 | Reynolds SR Harn DA | 1992 | Infect Immun1992，60，： | 1 |
| 10 | T and B cell epitope determination and analysis of multiple antigenic peptides for the Schistosoma mansoni experimental vaccine trios-phosphate isomerase显示文摘 | Reynolds SR Dhal CE Harn DA | 1994 | J Immunol1994，152，1： | 1 |
| 11 | Isotype responses to candidate vaccine antigens in protective sera obtained from mice vaccinated with irradiated cercariae of Schistosoma mansoni显示文摘 | Richter D Incani RN Harn DA | 1993 | Infect Immun1993，61，： | 1 |
| 12 | Schistosom a mansoni: genetic restriction and cytokine profile of the CD4^+ T helper cell response to dominant epitope peptide of major egg antigen Sm-p40显示文摘 | Hernandez H J Edson CM Harn DA | 1998 | Exp Parasitol1998，90，1： | 1 |
| 13 | Protective immunity induced with 23 kDa membrane protein DNA vaccine of Schistosoma japonicum Chinese strain in infected C57 BL/6 mice 显示文摘 | Ren J Harn DA | 2003 | Southeast Asian J Trop Med Public Health2003，34，4： | 1 |
| 14 | Oligosaccharide specific induction of interleukin to production by B 220 c211s from schistosoma-infected mice: a mechanism for regulation of CD4 + cell subsets 显示文摘 | Vellupilai P and Harn DA | 1994 | Proc Natl Acad Sci USA1994，91，： | 1 |
| 15 | Schistosoma mansoni: detection by monoclonal antibody of a 22, 000 -dalton surface membrane antigen which may be blocked by host molecules on lung stage parasites显示文摘 | Harn DA Mitsuyama M Huguenel ED | 1985 | J Immunol1985，135，： | 1 |
| 16 | T and B cell epitope mapping of SM23, an integral membrane protein of Schistosoma mansoni显示文摘 | Reynolds SR Shoemaker CB Harn DA | 1992 | J Immunol1992，149，： | 1 |
| 17 | T and B cell epitope mapping of SM23,an integral membrane protein of Schistosoma mansoni显示文摘 | Reynolds SR Shoemaker CB Harn DA | 1992 | J Immunol1992，149，： | 1 |
| 18 | Canndidate vaccine antigens identified by antibodies from mice vaccinated with 15- or 50- kilarad-irradiated cercariae of Schistosoma mansoni 显示文摘 | Rich D Harn DA | 1993 | Infect Immunl1993，61，： | 1 |
| 19 | T and B epitope determination and analysis of multiple antigenic peptides for the Schistosoma mansoni experimental vaccine triose- phosphate isomerase显示文摘 | Dahl CE Harn DA | 1994 | J Immunol1994，152，1： | 1 |
| 20 | T and B cell epitope determination and analysis of multiple antigenic peptides for the Schistosoma mansoni experimental vaccine trios-phosphate isomerase显示文摘 | Reynolds SR Dhal CE Harn DA | 1994 | J Immunol1994，152，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员