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| 1 | 日本血吸虫中国大陆株TPI DNA疫苗诱导BALB/c小鼠保护性免疫力的研究显示文摘目的 研究日本血吸虫中国大陆株磷酸丙糖异构酶 (Sj CTPI) DNA疫苗诱导 BAL B/ c小鼠的保护性免疫作用。方法 将 39只 BAL B/ c小鼠随机分成 3组 :A组 (对照组 ) ,肌注 pc DNA3.1质粒 DNA10 0 μg/鼠 ;B组 (实验组 ) ,每鼠肌注 pc DNA3.1- Sj CTPI10 0 μg;C组 (加强组 ) ,每鼠肌注 pc DNA3.1- Sj CTPI10 0 μg及 pc DNA3.1- P35和 pc DNA3.1-P40的混合物 10 0 μg。每两周免疫 1次 ,共计 3次。末次免疫 4周后 ,每鼠用 45条尾蚴进行攻击 ,45 d后剖杀小鼠 ,计数成虫以及每鼠肝组织内虫卵数 ;于攻击前每组各取两只小鼠采用 51 Cr释放法测定 Sj CTPI介导的特异性细胞毒作用 ;EL ISA检测攻击前后的抗 TPI的抗体水平。 结果 用 EL ISA检测抗 TPI抗体的结果表明 ,攻击前 A组的 10份血清均为阴性 ,B组 5 / 10份血清出现弱阳性 ,C组也有 6 / 10份血清出现弱阳性反应。51 Cr释放法测定细胞毒活性结果表明 ,A、B、C组细胞毒活性分别为 9.1%、2 7.6 %和 5 4.4%。与对照组相比 ,实验组的减虫率、减卵率分别为 30 .2 %、5 2 .9% ;加强组的减虫率、减卵率分别为 32 .7%、47.0 %。 结论 进一步证实了 Sj CTPI DNA疫苗作为抗血吸虫病核酸疫苗的潜力。 | 司进 朱荫昌 Harn DA 殷旭仁 余传信 何伟 任建功 华万全 | 2001 | 中国寄生虫病防治杂志2001，14，3： | 20 |
| 2 | Mechanisms inactivating the gene for E-cadherin in sporadic gastric carcinomas显示文摘瞄准:为了学习 CDH1/E-cadherin (电子恶棍) 的角色,基因改变介绍在胃的癌(GC ) 在激活在 CDH1 的机制包括变化,杂合现象(LOH ) 的损失,倡导者多型性和 hypermethylation。方法:标本与 GC 从 70 个病人通过手术被收集。Allelotyping PCR 和定序的 LOH,使中毒的高压力液相色谱法和 DNA 的察觉,限制碎片长度多型性分析,甲基化 specific PCR,和免疫组织化学的染色被使用。结果:倡导者多型性不是在激活的电子恶棍的主要机制。仅仅一截断变化在一个弥漫的类型肿瘤(3%) 被发现。LOH 和倡导者 hypermethylation 是在激活的电子恶棍的主要机制,但是有趣地,在 CDH1 的肿瘤和 hypermethylation 在突变而产生之遗传的损失(LOH ) 的部分之间有一个否定协会。因此 LOH 和 hypermethylation 是涉及 GC 的二条不同致瘤的小径。结论:给体细胞突变是极其低的,在 LOH 和 hypermethylation 之间的关系是反的调查结果,这三个因素的任何二联合不能在激活完成 CDH1 的古典二点击的假设。因此,在 transcriptional 水平或在 translational 以后水平操作的另外的机制可能被要求在激活导致 E-cadherin。 | Yao-Chi Liu Chen-Yang Shen Hurng-Sheng Wu Tsai-Yuan Hsieh De-Chuan Chan Cheng-Jueng Chen Jyh-Cherng Yu Cheng-Ping Yu Horng-Jyh Harn Peng-Jen Chen Chung-Bao Hsieh Teng-Wei Chen Huan-Mieng Hsu | 2006 | World Journal of Gastroenterology2006，12，14： | 16 |
| 3 | Helicobacter pylori infection in relation to E-cadherin gene promoter polymorphism and hypermethylation in sporadic gastric carcinomas显示文摘AIM: To study Helicobacter pylori (H pylori) infection in relation to E-cadherin (E-cad) promoter polymorphism and hypermethylation in GCs.METHODS: Specimens were taken from representative cancerous lesions and adjacent non-cancerous epithelia of 67 resected GCs. Hpyloriwas detected by real-time PCR of the cagA gene from non-neoplastic epithelium.E-cad promoter polymorphism and hypermethylation were determined by restriction fragment length polymorphism analysis and methylation-specific PCR, respectively. Expression of E-cad protein was determined by immunohistochemistry.RESULTS: Hpyloriwas found in 57% of patients with GC.H pylori infection was more frequently found in tumors with the -160C/C genotype than those with the -160C/A and -160A/A genotypes (74% vs47%, P = 0.02). Hpylori infection was associated with E-cad methylation in nonneoplastic epithelium; however, no significant difference in H pylori was observed between methylated and unmethylated cancerous lesions.CONCLUSION: Patients with the -160C/C genotype might require Hpyloriinfection to promote the inactivation of CDH1, suggesting that Hpylori infection might affect GC in an initial stage because polymorphism is germ line.Mechanism of hypermethylation of CDH1 promoter in GC is complex, and Hpyloriinfection might affect it in an initial stage. | Yao-Chi Liu Chen-Yang Shen Hurng-Sheng Wu De-Chuan Chan Cheng-Jueng Chen Jyh-Cherng Yu Cheng-Ping Yu Horng-Jyh Harn Rong-Yaun Shyu Yu-Lueng Shih Chung-Bao Hsieh Huan-Mieng Hsu | 2005 | World Journal of Gastroenterology2005，11，33： | 8 |
| 4 | Real-time PCR diagnosis of Schistosoma japonicum in low transmission areas of China显示文摘Background:Schistosomiasis in the People’s Republic of China(PRC)can be traced back to antiquity.In the past 60 years,the Chinese government has made great efforts to control this persistent disease with elimination slated by 2020 through the implementation of a comprehensive control strategy.This strategy aims to reduce the role of bovines and humans as sources of infection as a pre-requisite for elimination through transmission interruption.The goal of elimination will be achievable only by the implementation of a sustainable surveillance and control system,with sensitive diagnosis a key feature so that the true disease burden is not underestimated.Currently used diagnostics lack the necessary sensitivity to accurately determine the prevalence of Schistosoma japonicum infection in areas with low infection intensities.It is of critical importance to find and treat people and to identify animals with low-level infections if the National Control Programme for China is to achieve schistosomiasis elimination.Methods:We evaluated a real-time polymerase chain reaction(qPCR)assay using 633 human stool samples collected from five villages in Hunan,Anhui,Hubei,and Jiangxi provinces,and 182 bovine(70 cattle and 112 buffalo)stool samples obtained from four villages in Hunan,Anhui,and Jiangxi provinces in the PRC.All stool samples were subjected to the miracidium hatching test(MHT,a diagnostic procedure used in the National Schistosomiasis Control Programme)and the qPCR assay.Samples positive by MHT were subjected to either the Kato-Katz technique for humans,or the formalin-ethyl acetate sedimentation-digestion(FEA-SD)procedure for bovines,to determine infection intensities.Results:The qPCR assay exhibited a high level of sensitivity in the detection of S.japonicum infections.With both the human and bovine samples,a significantly higher prevalence was determined using the qPCR assay(11.06%humans,24.73%bovines)than with the MHT(0.93%humans,7.69%bovines).The animal contamination index(calculated using data obtained with the qPCR technique)for all positive bovines was 27618000 eggs per day,indicating a considerable amount of environmental egg contamination that would be underestimated using less sensitive diagnostic procedures.Conclusions:The qPCR assay we have evaluated will be applicable as a future field diagnostic and surveillance tool in low-transmission zones where schistosomiasis elimination is targeted and for monitoring post-intervention areas to verify that elimination has been maintained. | Pei He Catherine A.Gordon Gail M.Williams Yuesheng Li Yuanyuan Wang Junjian Hu Darren J.Gray Allen G.Ross Donald Harn Donald P.McManus | 2018 | Infectious Diseases of Poverty2018，7，1： | 7 |
| 5 | n-Butylidenephthalide induced apoptosis in the A549 human lung adenocarcinoma cell line by coupled down-regulation of AP-2α and telomerase activity显示文摘 | Chyou-wei WEI Chai-ching LIN Yung-luen YU Chai-yi LIN Po-cheng LIN Min-tze WU Cheng-jueng CHEN Wen-liang CHANG Shinn-zong LIN Yi-lin Sophia CHEN Horng-jyh HARN | 2009 | Acta Pharmacologica Sinica2009，30，9： | 5 |
| 6 | Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti- aging effects in D-galactose aging mouse model显示文摘 | Sung-liang YU Shih-bin LIN Yung-luen Yu Min-hui CHIEN Kuo-jung SU Ching-ju LIN Tzong-der WAY Giou-teng YIANG Chai-ching LIN De-chuan CHAN Horng-jyh HARN Yi-lin Sophia CHEN | 2010 | Acta Pharmacologica Sinica2010，31，12： | 3 |
| 7 | 一个与辣椒细胞核雄性不育性状连锁的高分辨率熔解曲线(HRM)标记的开发显示文摘雄性不育性状被用在植物育种中,以降低种子生产成本。雄性不育分为细胞核雄性不育(GMS)和细胞质雄性不育(CMS)2种类型,在辣椒商业育种中都有应用。因为缺少恢复源,细胞质雄性不育系统在诸如甜椒等一些辣椒品种上并不适用。因此,细胞核雄性不育对于甜椒的种子生产而言就非常重要。本研究构建了一个F2分离群体,利用集群分离分析法(BSA)和扩增片段长度多态性(AFLP)技术获得了一个与甜椒细胞核雄性不育连锁的标记。我们分析了1024对AFLP引物,发现Eco RI ACG/MseIGTT引物扩出了多态性。对多态性AFLP标记所扩增的395bp片段重新设计引物,进行高分辨率熔解曲线分析(HRM)。在92个F2单株中,有87个单株的性状和HRM标记表型相一致。这个HRM标记被定位在AC99连锁图的第5号染色体上。 | Heung-Ryul Lee Hye Jung An Dong Cheol Yang Soon Ho Choi Hyoun-Joung Kim Han Gheol Rhee Chee Hark Harn 孔秋生 | 2012 | 辣椒杂志2012，，4： | 3 |
| 8 | 日本血吸虫SjCTPI-Hsp70 DNA疫苗与白细胞介素12对水牛的联合免疫保护作用研究显示文摘目的探讨中国大陆株日本血吸虫磷酸丙糖异构酶-热激蛋白(SjCTPI-Hsp70)DNA疫苗联合佐剂白细胞介素-12(IL-12)质粒DNA对水牛的免疫保护作用。方法实验采用双盲法,所用疫苗及制剂均在实验结束后解码。将购自非血吸虫病流行区45头8~10月龄健康水牛随机分为A组(SjCTPI-Hsp70+IL-12,300μg)、B组(SjCTPI+IL-12,300μg)和C组(空质粒pVAX+IL-12,300μg)等3组(每组15头),每头牛分别经肩部肌肉注射免疫3次,每次间隔28d。末次免疫后28d,每头牛经大腿内侧皮肤感染日本血吸虫尾蚴1000条。解剖前2d及当天分别收集粪便1次,用定量法计数虫卵和毛蚴。攻击感染后56d解剖,用生理盐水经胸主动脉灌冲法收集、计数成虫,检测每克肝组织虫卵数。结果A、B组减虫率分别为51.2%和41.5%(χ2=1.89,P>0.05),减雌虫率分别为48.9%和44.7%(χ2=0.35,P>0.05),减粪卵率分别为52.1%和38.3%(χ2=3.84,P<0.05),减毛蚴率为52.1%和33.2%(χ2=7.30,P<0.01)及减肝卵率为61.5%和42.0%(χ2=7.61,P<0.01)。结论用SjCTPI-Hsp70+IL-12免疫水牛可获得一定的免疫保护性作用。 | 喻鑫玲 何永康 熊铁 赵雅琴 石孟芝 周杰 刘宗传 罗新松 付晓 贺宏斌 DA HARN 李岳生 | 2006 | 中国寄生虫学与寄生虫病杂志2006，24，6： | 3 |
| 9 | 强抗泄漏的无条件安全动态秘密共享方案显示文摘(t,n)门限秘密共享中,分发者将秘密S分成n个秘密份额,使得任意不少于t个秘密份额可以成功重构秘密S,而小于门限t的秘密份额无法获得有关秘密的任何信息.为提高份额的机密性,减少验证份额过程所需的计算开销,增强秘密共享方案的防欺骗性、安全性、实用性以及抗泄漏性,本文提出了一种强抗泄漏的无条件安全动态秘密共享方案:(1)方案引入信息传递环和插值因子等技术作为重构通信方式和重构信息,有效提高份额的机密性;(2)方案分别为每个参与者分配一对验证钥以验证重构秘密的一致性,避免验证秘密份额的复杂过程,方案与可验证秘密共享方案具有相同的抗欺骗能力,可有效防止n-1参与者联合欺骗,较一般防欺骗方案(达到n/2或n/3防欺骗能力)有更强的抗欺骗能力;(3)方案安全性与敌手的计算能力无关,达到无条件安全性;(4)方案无需要更改秘密份额即可实现分享秘密的更新,达到动态性;(5)本文首次提出秘密共享中的最大泄漏率概念,用以描述秘密共享中最大可泄漏的秘密份额个数与总秘密份额个数的关系,可作为评价秘密共享机制中抗泄漏能力强弱的标准参数之一. | 张明武 陈泌文 李发根 蒋亚军 HARN Lein | 2016 | 密码学报2016，3，4： | 2 |
| 10 | Mesenchymal stem cells facilitate recovery from chemically induced liver damage and decrease liver fibrosis显示文摘 | Yao-Jen Chang Jen-Wea Liu Po-Cheng Lin Li-Yi Sun Chih-Wen Peng Geng-Hong Luo Tse-Min Chen Ru-Ping Lee Shinn-Zong Lin Horng-Jyh Harn Tzyy-Wen Chiou | 2009 | Life Sciences2009，，13： | 2 |
| 11 | 抗日本血吸虫卵单克隆抗体的制备和免疫化学的鉴定显示文摘用可溶性日本血吸虫卵抗原(SEA)免疫小鼠制备单克隆抗体,获得5株特异的抗日本血吸虫卵单抗。免疫球蛋白亚类鉴定均为IgG_1。免疫印迹法结果表明其所识别的日本血吸虫卵抗原的分子量分别为>200kDa、116kDa和22kDa。经两维免疫印迹法(2-Dimensional Western Blot),显示被单抗IE1识别的22kDa SEA等电点pI4.6—6之间至少有3个以上同晶型体(isomorphs)。放射免疫沉淀试验表明IE1可识别30kDa抗原。 | 许阿莲 周晓音 王炳夫 Donald A Harn | 1992 | 中国寄生虫学与寄生虫病杂志1992，10，1： | 2 |
| 12 | Russell body duodenitis with immunoglobulin kappa light chain restriction显示文摘Russell bodies are eosinophilic intracytoplasmic globules which are likely the result of disturbed secretion of immunoglobulins that accumulate within the plasma cell. Russell body collections have been identified within the stomach, known as Russell body gastritis. Similar lesions within the duodenum are referred to as Russell body duodenitis, which is rare. Several Russell body gastritis case reports are associated with Helicobacter pylori. However, the etiology of Russell body duodenitis remains unclear. Here we report the first case of Russell body duodenitis with immunoglobulin light chain restriction in a background of peptic duodenitis. | William R Munday Lucy Harn Kapur Mina Xu Xuchen Zhang | 2015 | World Journal of Gastrointestinal Endoscopy2015，7，1： | 2 |
| 13 | A review of the recent research on vibration energy harvesting via bistable systems显示文摘 | R L Harne K W Wang | 2013 | Smart Materials and Structures2013，，2： | 2 |
| 14 | Design of generalized EIGamal type digital signature schemes based on discrete logarithm显示文摘 | L Harn Y Xu | 1994 | Electronics Letters1994，30，24： | 1 |
| 15 | New scheme for digital multisignatures显示文摘 | Harn L Kiesler T | 1989 | Electronics Letters1989，25，15： | 1 |
| 16 | New digital signature based on discrete logarithm显示文摘 | Harn L Kiesler T | 1994 | Electronics Letters1994，30，5： | 1 |
| 17 | Fair reconstruction of a secret显示文摘 | LIN Hung-yu HARN L | 1995 | Information Processing Letters1995，55，： | 1 |
| 18 | Efficient Sharing (Broadcasting) of Multiple Secret 显示文摘 | Harn L | 1995 | IEE Proceedings-Computers and Digital Techniques1995，142，3： | 1 |
| 19 | Relation between the incidence and level of pigment cell antibodies and disease activity in vitiligo显示文摘 | Harning R Cui J Bystryn JC | 1991 | J Invest Dermatol1991，97，： | 1 |
| 20 | Efficient sharing (broadcasting) of multiple secrets显示文摘 | | 1995 | IEE Computers and Digital Techniques1995，142，3： | 1 |
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