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| 1 | Stability of randomly amplified polymorphic DNA fingerprinting in genotyping clinical isolates of Helicobacter pylori显示文摘AIM: Hpylorigenomes are highly diversified. This project was designed to genotype Hpyloriisolates by the polymerase chain reaction (PCR)-based randomly amplified polymorphic DNA (RAPD) fingerprinting technique and to verify its stability by Southern blotting and DNA sequencing.METHODS: Clinical isolates of Hpyloriwere cultured from gastric antra and cardia of 73 individuals, and genomic DNA was prepared for each isolate. RAPD was carried out under optimized conditions. 23S rDNA was regarded as an internal control, and a 361 bp rDNA fragment (RDF) was used as a probe to screen the RAPD products by Southern blotting.Ten RDFs from different clinical isolates and the flanking regions (both upstream and downstream) of four RDFs were amplified and sequenced.RESULTS: Hpyloriisolates from different individuals had different RAPD profiles, but the profiles for isolates cultured from different gastric sites of a given individual were identical in all but one case. Isolates from 27 individuals were RDF positive by Southern blotting. Sequences of the RDFs and their flanking regions were almost the same between the RDF positive and negative isolates as determined by Southern blotting. There was no binding site for random PCR primer inside the sequences.CONCLUSION: RAPD is very useful in genotyping H pylori grossly on a large scale. However, it seems unstable in amplification of low yield fragments, especially those that do not appear as visible bands on the agarose gel stained with EB, since the palmer is partially matched to the template. | Feng-Chan Han Han-Chong Ng Bow Ho Department of Microbiology,Faculty of Medicine,National University of Singapore,5 Science Drive 2,Singapore 117595,Republic of Singapore | 2003 | World Journal of Gastroenterology2003，9，9： | 9 |
| 2 | CarbonisotopiccompositionandfluxofparticulateorganicmatterintheChangjingRiver显示文摘CarbonisotopiccompositionandfluxofparticulateorganicmatterintheChangjingRiverCaiDeling,HanYibing(ReceivedJune5,1996;acceptedJ... | Cai Deling Han Yibing(Received June 5, 1996 acceptedJuly 13, 1997) | 1998 | Acta Oceanologica Sinica1998，17，3： | 5 |
| 3 | Identification of H.pyloristrain specific DNA sequences between two clinical isolates from NUD and gastric ulcer by SSH显示文摘AIM: The genomes of Helicobacter pylori(H. pylori) from different individuals are different. This project was to identify the strain specific DNA sequences between two clinical H. pylori isolates by suppression subtractive hybridization (SSH).METHODS: Two clinical H. pylori isolates, one from gastric ulcer (GU, tester) and the other from non-ulcer dyspepsia (NUD, driver), were cultured and the genomic DNA was prepared and submitted to AluⅠdigestion. Then two different adaptors were ligated respectively to the 5′-end of two aliquots of the tester DNA fragments and SSH was made between the tester and driver DNA. The un-hybridized tester DNA sequences were amplified by two sequential PCR and cloned into pGEM-T-Easy Vector. The tester strain specific inserts were screened and disease related DNA sequences were identified by dot blotting.RESULTS: Among the 240 colonies randomly chosen, 50contained the tester strain specific DNA sequences. Twenty three inserts were sequenced and the sizes ranged from 261 bp to 1 036 bp. Fifteen inserts belonged to the H.pylori plasmid pHPO100 that is about 3.5 kb and codes a replication protein A. Other inserts had patches of homologous to the genes of H. pylori in GenBank. Various patterns of dot blots were given and no GU strain unique DNA sequences were found when 4 inserts were used as probes to screen the genomic DNA from 27 clinical isolates, 8 from GU, 12 from duodenum ulcer (DU), 4 from GU-DU, 2 from NUD and 1from gastric cancer (GC). But a 670 bp DNA fragment (GU198)that was a bit homologous to the 3′-end of the gene of thymidylate kinase was positive in 7 GU strains (7/8), 3 GUDU strains (3/4) and 3 DU strains (3/12). A 384 bp fragment (GU79) of the replication gene A (repA) was positive only in 4 H, pylori isolates, 2 from GU and 2 from GU-DU.CONCLUSION: Differences exist in the genes of different H.pylori isolates. SSH is very effective to screen H. pylori strain specific DNA sequences between two clinical isolates,and some of these sequences may have clinical significance. | Feng-Chan Han Min Gong Han-Chong Ng Bow Ho, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117595, Republic of Singapore | 2003 | World Journal of Gastroenterology2003，9，8： | 5 |
| 4 | High spin states in stable nucleus ^(84)Sr显示文摘High spin states of 84Sr were populated through the reaction 70Zn(18O,4n)84Sr at 75 MeV beam energy.Measurement of excitation function,γ-γ coincidences,directional correlation from oriented state (DCO) ratios and γ-transition intensities were performed using eight anticompton HPGe detectors and one planar HPGe detector.Based on the measured results,a new level scheme of 84Sr was established in which 12 new states and nearly 30 new γ-transitions were identified in the present work.The positive-parity states of the new level scheme were compared with the results from calculations in the framework of the projected shell model (PSM).One negative-parity band was extended to spin Iπ=19-and it can be found that in the high spin states,the γ-transition energies show the nature of signature staggering.The negative-parity band levels are in good agreement with deformed configuration-mixing shell model (DCM) calculations. | KANG XuZhong1,2,SHEN ShuiFa1,2,3,4*,HAN GuangBing5,PAN Feng3,4,GU JianZhong6,DRAAYER J P4,WU XiaoGuang6,ZHU LiHua6 & WEN TingDun7 1 Key Laboratory of Nuclear Resources and Environment (East China Institute of Technology),Ministry of Education,Nanchang 330013,China 2 School of Nuclear Engineering and Technology,East China Institute of Technology,Fuzhou 344000,China 3 Department of Physics,Liaoning Normal University,Dalian 116029,China 4 Department of Physics and Astronomy,Louisiana State University,Baton Rouge,LA 70803-4001,USA 5 School of Physics,Shandong University,Jinan 250100,China 6 China Institute of Atomic Energy,Beijing 102413,China 7 Department of Physics,North University of China,Taiyuan 030051,China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，10： | 4 |
| 5 | Establishment of bovine prion peptide-based monoclonal antibodies for identifying bovine prion显示文摘To obtain high titer monoclonal antibodies(McAbs) which can react with mammalian prion protein(PrP),Balb/C mice were immunized with bovine(Bo) PrP peptide(BoPrP 209-228 aa) coupled to keyhole limpet hemocyanin(KLH).The hybridoma cell lines secreting monoclonal antibodies against the peptide were established by cell fusion and cloning.The obtained McAbs were applied to detect recombinant human,bovine and hamster PrP,cellular prion protein(PrPc) in normal bovine brain and pathogenic scrapie prion protein(PrPSc) accumulated in the medulla oblongata of bovine spongiform encephalopathy(BSE)specimen with Western blot and immunohistochemical detection,respectively.The current procedure might offer a simple,feasible method to raise high titer antibodies for studying biological features of PrP in mammals,as well as detection of transmissible spongiform encephalopathy(TSE) and diagnosis of BSE,in particular. | ZHAO Li1,HOU XinSheng5,JI Rong2,HAN ChunHui2,YU XiuPing3,HONG Tao4,5 1 Department of Laboratory Science of Microbiology,School of Public Health,Shandong University,Jinan 250012,China 2 Institute of Nutrition and Food Safety,Chinese Center of Disease Prevention and Control,Beijing 100021,China 3 Department of Microbiology,School of Medicine,Shandong University,Jinan 250012,China 4 Institute of Virus Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 100052,China 5 Institute of Biological Science and Technology,School of Science,Beijing Jiaotong University,Beijing 100044,China | 2009 | Science China(Life Sciences)2009，52，8： | 3 |
| 6 | A systematic investigation of high harmonic generation using mid-infrared driving laser pulses显示文摘We report on a systematic investigation of the influences of gas pressure,focal position and focusing geometry on high harmonic generation by use of mid-infrared femtosecond laser pulses. We also discuss the spatial characteristics of harmonics under different focusing conditions. By optimizing the parameters,we experimentally observed the generation of 1 kHz,low divergence coherent X-ray beams in the water-window region. | YAO JinPing 1,2 ,XIONG Hui 1 ,XU Han 1 ,FU YuXi 1,2 ,ZENG Bin 1,2 ,CHU Wei 1,2 ,CHENG Ya 1 ,XU ZhiZhan 1 ,LIU XiaoJun 3 & CHEN Jing 4,5 1 State Key Laboratory of High Field Laser Physics,Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Shanghai 201800,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China 3 State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences,Wuhan 430071,China 4 Institute of Applied Physics and Computational Mathematics,Beijing 100088,China 5 Center for Applied Physics and Technology,Peking University,Beijing 100084,China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，6： | 3 |
| 7 | Functional links between clustered microRNAs : suppression of cell-cycle inhibitors by microRNA clusters in gastric cancer显示文摘 | Kim Y K Yu J Han T 5 et ai | 2009 | Nucleic Acids Res2009，37，5： | 1 |
| 8 | Electrophysiological characteristics of the Marshall bundle in humans显示文摘 | Han 5 Joung B Scanavacca M ? | 2010 | Heart Rhylhm2010，7，6： | 1 |
| 9 | 查看详情显示文摘 | Zhao Y H Huang Z W Li A H Mu 5( Q Yang W M Hou H Han P D Zhang S Y | | 0，，： | 1 |
| 10 | Effect of Carbon Content on Cracking Phenomenon Occurring during Cold Rolling of Three Light-Welght Steel Plates 显示文摘 | Han 5 Shin 5 Lee 5 | 2011 | Metallurgical and Materials Transactions : A2011，42，1： | 1 |
| 11 | The genetic load for hereditary hearing impairment in Chinese population and its clinical implication显示文摘Objective To understand the genetic load in the Chinese population for improvement in diagnosis, prevention and rehabilitation of deafness. Methods DNA samples, immortalized cell lines as well as detailed clinical and audiometric data were collected through a national genetic resources collecting network. Two conventional genetic approaches were used in the studies. Linkage analysis in X chromosome and autosomes with microsatellite markers were performed in large families for gene mapping and positional cloning of novel genes. Candidate gene approach was used for screening the mtDNA 12SrRNA, GJB2 and SLC26A4 mutations in population -based samples. Results A total of 2,572 Chinese hearing loss families or sporadic cases were characterized in the reported studies, including seven X-linked, one Y-linked, 28 large and multiplex autosomal dominant hearing loss families, 607 simplex autosomal recessive hereditary hearing loss families, 100 mitochondrial inheritance families, 147 GJB2 induced hearing loss cases, 230 cases with enlarged vestibular aqueduct (EVA) syndrome, 169 sporadic cases with auditory neuropathy, and 1,283 sporadic sensorineural hearing loss cases. Through linkage analysis or sequence analysis, two X-linked families were found transmitting two novel mutations in the POU3F4 gene, while another X -linked family was mapped onto a novel locus, nominated as AUNX1 (auditory neuropathy, X-linked locus 1). The only Y-linked family was mapped onto the DFNY1 locus(Y-linked locus 1, DFNY1). Eight of the 28 autosomal dominant families were linked to various autosomal loci. In population genetics studies, 2,567 familial cases and sporadic patients were subjected to mutation screening for three common hearing loss genes: mtDNA 12S rRNA 1555G, GJB2 and SLC26A4. The auditory neuropathy cases in our samples were screened for OTOF gene mutations. Conclusions These data show that the Chinese population has a genetic load on hereditary hearing loss. Establishing personalized surveillance and prevention models for hearing loss based on genetic research will provide the opportunity to decrease the prevalence of deafness in the Chinese population. | WANG Qiu-ju 1,2, RAO Shao-qi 1, 3, GUO Yu-fen 4, LI Qing-zhong 5, ZHAO Hui 1, ZHAO Li-dong 1, YUAN Hu 1, ZONG Liang 1, LIU Qiong 1, ZHAO Ya-li 6, WANG Da-yong 1, HAN Ming-kun 1, JI Yu-bin 1, LI Jian-qiang 1, LAN Lan 1, YANG Wei-yan 1, SHEN Yan 2,6, HAN Dong-yi 1 1 Department of Otorhinolaryngology-Head and Neck Surgery, and Institute of Otolaryngology, Chinese People’s Liberation Army General Hospital, Beijing, 100853 China 2 Chinese National Human Genome Center, Beijing, 100176 China 3 Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, China 4 Department of Otorhinolaryngology, Head and Neck Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, China 5 Department of Otolaryngology, EYE & ENT hospital of Fudan University, Shanghai, 200031,China 6 Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005 China | 2009 | Journal of Otology2009，4，2： | 1 |
| 12 | 5mad7 acts as a negative regula- tor of the epidermal growth factor (EGF) signaling pathway in breast cancer cells显示文摘 | Kim 5 Han J7 Lee 5K | 2012 | Cancer letters2012，314，2： | 1 |
| 13 | Expression of synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B suhunit of Escherichia coli heat-labile enterotoxin in tobacco plants显示文摘 | Kang TJ Han 5 C Yang M 5 | 2006 | Protein Expression and Purification2006，46，1： | 1 |
| 14 | A Chemical Route to Gra-phene for Device Applications显示文摘 | Gilje 5 Han S Wang M | 2007 | Nano Lett2007，7，11： | 1 |
| 15 | DISTRIBUTION OF Ce AND S IN Fe-C-Si ALLOY AND THEIR INFLUENCE ON MORPHOLOGY OF GRAPHITE显示文摘Electrolytic extraction-radiometry and microradioautography techniques were used to studythe distribution of nodulizing element Ce and anti-nodulizing element S in phases of highpurity Fe-C-Si alloy.The results show that,besides forming inclusions,most of Ce atomsconcentrate in graphite firstly.As the Ce content increases,the concentration of Ce ingraphite tends to saturate,relatively large amount of Ce dissolves in the alloy.Usually,thesulphur also concentrates in graphite.However,it may exist in the form of FeS at theeutectic colony boundaries,if the concentration of S is at a quite high level.Very littleamount of S dissolves in the alloy.The morphology of graphite depends upon the degree ofsaturation of Ce.It appears as nodular when Ce was saturated and,as vermicular while itwas semi-saturated. | DU Weixi HAN Qiyong JIANG Qing ZHANG Zhongzhi ZHONG Weizhen LIU Shaoshou Jilin University of Technology,Changchun,China in revised form 3 April 19871 Beijing University of Science and Technology | 1989 | Acta Metallurgica Sinica(English Letters)1989，2，8： | 0 |
| 16 | Nd isotope as the tracer of seawater evolution of early Miocene in the eastern Pacific Ocean显示文摘Fifty-six samples of nannofossil ooze were collected from Core PC5794 in the northern equatorial Pacific at 5 cm intervals. With the methods of mass spectrometer (VG354) and ICP, the Nd isotopic compositions (εNd(t)), Mn contents and Mg/Sr ratios of carbonate phase have been analyzed. CaCO3 contents of bulk sediments were obtained by dissolution of 0.5 mol/L HCl. Based on these data, the high-resolution εNd(t) profile of seawater in early Miocene with core depth(or time) have been established. The values of εNd(t) range from -6.2 to -2.97 and 4 fluctuation cycles existed during 24.06-22.02 Ma. 4 low εNd(t) values (about -6.4) correspond to high CaCO3 contents, which implicates that there were 4 cold epochs or 4 times of Antarctic Bottom Water activity. They occurred at the time of 24.06 Ma, 23.85 Ma,22.88 Ma and 22.26 Ma, respectively. High εNd(t) values correspond to the high Mn contents and high values of Mg/Sr ratio, which indicates the existence of 4 intense hydrothermal activity periods during 24.06-22 Ma, the durations of them are 4.05-23.98 Ma, 23.69-23.15 Ma, 22.74-22.37 Ma and 22.06-22.02 Ma, respectively. | LIU Jihua~(1,2,3), CHEN Lirong~1, WANG Yingxi~4 & HAN Jianxiu~5 1. Institute of Oceanology, Chinese Acadcmy of ences, Qingdao 266071. China 2. Key Lab of Marine Sedimentology and Environmental Geology, First Institute of Oceanograpgy, State Oceanic Administration, Qingdao 266061. China 3. Graduate School, Chinese Academy of Sciences, Beijing 100039. China 4. Modern Analysis Center, Nanjing University. Nanjing 210093. China 5. Guangzhou Marine Investigation Bureau, Guangzhou 510760. China | 2003 | Chinese Science Bulletin2003，48，B06： | 0 |
| 17 | Asynchronous Nested Optimization Algorithms and Their Parallel Implementation显示文摘Large scale optimization problems can only be solved in an efficient way, if their special structure is taken as the basis of algorithm design. In this paper we consider a very broad class of large-scale problems with special structure, namely tree structured problems. We show how the exploitation of the structure leads to efficient decomposition algorithms and how it may be implemented in a parallel environment. | Hans W. Moritsch, G.Ch. Pflug, M. Siomak Department of Statistics and Decision Support Systems,University of Vienna, Universitaetsstrasse 5 A\|1090 Vienna, Austria | 2001 | Wuhan University Journal of Natural Sciences2001，6，Z1： | 0 |
| 18 | Active control of interior noise within an irregular enclosure under the cooperation of point force and Incident wave显示文摘A new modeling method is developed for the active control of interior noise within an irregular three-dimensional cavity under the cooperation of point force and incident wave. The validity of this method is verified by a regular cuboid enclosure. With global and local performance functions, good results are obtained in the active control of noise within the irregular enclosure according to numerical investigations. | GENG Houcai RAO Zhushi HAN Zushun ZHANG Hualiang(1 Shanghai Jiao Tong University Shanghai 200030)(2 Jiangnan Shipgard (Group) Co. Ltd. Shanghai 200030)Received Jan. 3, 2001Revised Apr. 5, 2001 | 2002 | Chinese Journal of Acoustics2002，21，4： | 0 |
| 19 | Screening of GJB2 mutations in Chinese population显示文摘The GJB2 gene(connexin 26) has been shown to be responsible for DFNB1 and DFNA3. We screened the GJB2 gene in 488 patients with prelingual deafness(Group 1), 124 with postlingual deafness(Group 2), and 117 normal hearing subjects(Group 3). We found that, in Group 1, 65 patients(13.32%) were homozygotes or compound heterozygotes and 51 patients (10.45%) carried a single pathogenic mutation. The 235delC mutation was the most frequent mutation, accounting for 73.22% of the known pathogenic alleles in Group 1. No homozygotes or compound heterozygotes were detected in Group 2 or Group 3. Some postlingual deaf patients (2.42%) and normal hearing subjects(4.27%) were 235delC carriers. Our preliminary data indicate that 235delC, the most frequent mutation identified in this study, is a major cause for prelingual deafness. | HAN Ming-kun1, HAN Dong-yi1, GUO Yu-fen2, LI Qing-zhong3, ZHAO Ya-li1, 4, RAO Shao-qi1, 5, 6, YUAN Hu1, ZONG Liang1, GUAN Jing2, XU Bai-cheng2, WANG Da-yong1, LAN lan1, WANG Qiu-ju1, 7 1 Department of Otorhinolaryngology/ Head and Neck Surgery, and Institute of Otolaryngology, China People Liberation Army General Hospital, Beijing, China 2 Department of Otorhinolaryngology/ Head and Neck Surgery, the Second Affiliated Hospital of LanZhou University, Lanzhou, China 3 Deparment of Otlolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, China 4 Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China 5 Departments of Cardiovascular Medicine and Molecular Cardiology, the Cleveland Clinic Foundation, Cleveland, Ohio, USA 6 Department of Bioinformatics, Harbin Medical University, Harbin, China 7 China National Human Genome Center, Beijing, China | 2007 | Journal of Otology2007，2，1： | 0 |
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