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| 1 | TNF-α Induces Transient Resistance to Fas-Induced Apoptosis in Eosinophilic Acute Myeloid Leukemia Cells显示文摘Tumor necrosis factor α (TNF-α) has been recognized as an activator of nuclear factor κB (NF-κB), a factor implicated in the protection of many cell types from apoptosis. We and others have presented evidence to suggest that Fas-induced apoptosis may be an important aspect of the resolution of inflammation, and that delayed resolution of inflammation may be directly associated with NF-κB-dependent resistance to Fas. Because TNF-α activates NF-κB in many cell types including inflammatory cells such as eosinophils, we examined effects of TNF-α signaling on the Fas-mediated killing of an eosinophilic cell line AML14. While agonist anti-Fas (CH11) treatment induced apoptosis in AML14 cells, no significant cell death occurred in response to TNF-α alone. Electrophoretic mobility shift assay (EMSA) revealed that TNF-α induced NF-κB transactivation in AML14 cells in a time- and dose-dependent fashion, and subsequent supershift assays indicated that the translocated NF-κB was the heterodimer p65 (RelA)/p50. Pre-treatment of cells with TNF-α dramatically decreased the CH11-induced cell death in a transient fashion, accompanied by suppression of activation of caspase-8 and caspase-3 activation. Inhibition of NF-κB transactivation by inhibitors, BAY 11-7085 and parthenolide, reversed the suppression of Fas-mediated apoptosis by TNF-α. Furthermore, TNF-α up-regulated X-linked inhibitor of apoptosis protein (XIAP) transiently and XIAP levels were correlated with the temporal pattern of TNF-α protection against Fas-mediated apoptosis. This finding suggested that TNF-α may contribute to the prolonged survival of inflammatory cells by suppression of Fas-mediated apoptosis, the process involved with NF-κB transactivation,anti-apoptotic XIAP up-regulation and caspase | Yimin Qin Sogyong Auh Lyubov Blokh Catherine Long Isabelle Gagnon Kimm J. Hamann | 2007 | Cellular & Molecular Immunology2007，4，1： | 19 |
| 2 | 慢性胰岛素刺激对胰岛素受体后不同信号转导途径的下降调节显示文摘目的 研究胰岛素慢性刺激对人肝癌细胞株 (HepG2 )胰岛素受体后不同信号转导途径的影响。方法 HepG2 细胞在无血清条件下与不同浓度的胰岛素 ( 0~ 10 0nmol/L)温育 16h ,然后用 10 0nmol/L胰岛素急性刺激 1min。这些细胞的溶解物中的胰岛素受体 β亚单位 (IRβ) ,胰岛素受体底物 (IRS) 1,IRS 2 ,磷酯酰肌醇 3激酶 (PI3K)的调节亚单位P85 ,有丝分裂原激活蛋白激酶 (MAPK)的蛋白表达水平和MAPK的磷酸化水平通过Western免疫印迹法测定 ,IRβ、IRS 1/ 2的蛋白磷酸化水平以及IRS 1/ 2与P85的结合反应用特异性抗体的免疫沉淀法。结果 胰岛素 1min急性刺激能迅速导致IRβ、IRS 1、IRS 2的酪氨酸磷酸化和MAPK的磷酸化 ,以及IRS 1( 2 )与P85的相互作用而激活PI3K。高浓度胰岛素慢性刺激显著降低了IRβ、IRS 1和IRS 2的酪氨酸磷酸化。细胞用 10 0nmol/L胰岛素预温育 16h后 ,IRβ、IRS 1和IRS 2的磷酸化水平降至最低值 ,分别为对照水平的 2 2 .2 % (P <0 .0 1)、10 .9% (P <0 .0 1)和 2 2 .0 % (P<0 .0 1) ,与IRS的磷酸化变化相平行 ,IRS 1和IRS 2与PI3K的相互作用分别降低至对照水平的 3 4.3 %(P <0 .0 1)和 3 0 .0 % (P <0 .0 1) ,MAPK的磷酸化水平降低至对照水平的 16.4% (P <0 .0 1)。 | 袁莉 Reinhard ZIEGLER Andreas HAMANN | 2003 | 中华内分泌代谢杂志2003，19，4： | 5 |
| 3 | 荨麻疹性皮炎的定义:皮肤过敏反应模式的一种亚型显示文摘Background: Urticarial dermatitis may represent a useful term for a subset of a reaction pattern designated most commonly as dermal hypersensitivity by pathologists. The term is not commonly used, and requires definition to determine whether it is clinically relevant. Objectives: To define urticarial dermatitis and distinguish it from other urticarial reaction patterns and to review the frequency with which dermatologists can recognize clinical settings that match the biopsy findings of urticarial dermatitis. Design: Retrospective analysis of clinical and/or histological diagnosis of urticarial dermatitis, applying strict histological criteria in a center using urticarial dermatitis as a diagnostic term in 190 archived reports. Setting: Tertiary referral dermatopathology service reporting for dermatological practices in Sydney, Australia. Main Outcome Measures: The correlation between clinical and histological diagnoses of urticarial dermatitis and alternate diagnoses was analyzed. The frequency of positive immunofluorescence findings for bullous pemphigoid was determined in a subset of patients with urticarial dermatitis in whom this test was ordered to exclude prodromal bullous pemphigoid. Results: Urticarial dermatitis was the histological diagnosis in at least 1 biopsy result in 148 patients, and matched the provisional clinical diagnosis in 49 (33.1% ) patients. Urticarial dermatitis was the only diagnosis provided in 21 patients. The main alternate clinical diagnoses provided were early bullous pemphigoid or dermatitis herpetiformis (47 patients [31.8% ]), dermatitis (39 patients [26.4% ]),drug reaction (35 patients [23.6% ]), urticarial vasculitis (24 patients [16.2% ]), and urticaria (12 patients [8.1% ]). In 91 patients with a clinical diagnosis of urticarial dermatitis, the histological diagnosis in at least 1 biopsy result was matched in 49 patients (53.8% ); other histological diagnoses included dermatitis (21 patients [23.1% ]), papular urticaria (12 patients [13.2% ]), drug reaction (6 patients [6.6% ]), and urticaria (3 patients [3.3% ]). Review of 38 direct immunofluorescent results for prodromal bullous pemphigoid and a biopsy finding of urticarial dermatitis revealed only 3 positive results (7.9% ).Conclusions: Urticarial dermatitis seems to be a useful histological and clinical term for a subset of the dermal hypersensitivityreaction pattern. Although the clinical presentation is not restricted to a specific entity, eczema and drug reactions seem to be the most frequent clinical associations; and in a subset of patients, urticarial dermatitis remains as a recognizable reaction pattern. Urticarial dermatitis without eosinophilic spongiosis is not a reliable indicator for bullous pemphigoid, because the findings of immunofluorescence are often negative. | Kossard S. Hamann I. Wilkinson B. 潘敏 | 2006 | 世界核心医学期刊文摘(皮肤病学分册)2006，2，3： | 3 |
| 4 | Application of the European flaw assessment procedure SINTAP to pipes显示文摘 | Zerbst U Hamann R Wohlschlegel A | 2000 | The International Journal of Pressure Vessels and Piping2000，77，11： | 2 |
| 5 | 二甲双胍对磷酸烯醇丙酮酸羧激酶基因表达调节的研究显示文摘目的 通过观察抗高血糖药物二甲双胍对肝糖异生中的关键限速酶磷酸烯醇丙酮酸羧激酶 (PEPCK)基因表达的调节以及胰岛素信号转导抑制剂的影响 ,探讨二甲双胍在肝细胞中作用的分子机制。方法 培养的小鼠肝癌细胞系 (H4IIE)用 0 1mmol/L二甲双胍温育 16h ,并加用不同的调节因子 ,如胰岛素、环磷酸腺苷 (cAMP)、地塞米松、胰岛素信号转导抑制剂等。PEPCK的mRNA水平通过Northernblot分析。结果 治疗浓度 0 .1mmol/L二甲双胍显著降低了基础PEPCK的mRNA水平约 75 % (P <0 .0 1) ,对cAMP和地塞米松刺激的PEPCKmRNA表达 ,二甲双胍单独作用无显著性效果 ,但通过与胰岛素的联合作用可抑制cAMP和地塞米松刺激的PEPCK的基因表达。当培养液中加入 0 .1nmol/L低浓度胰岛素与二甲双胍共同温育时 ,cAMP和地塞米松引起的PEPCKmRNA表达显著降低了 94 % (P <0 .0 1)。胰岛素信号转导抑制剂如磷脂酰肌醇 3激酶 (PI3K)抑制剂渥蔓青霉素(wortmannin)和有丝分裂原激活蛋白激酶 (MAPK)抑制剂UO12 6对二甲双胍的作用无显著影响 ,但wortmannin显著阻止了胰岛素对PEPCK基因表达的调节作用。结论 二甲双胍可通过独立的非胰岛素信号转导途径或是与胰岛素的联合作用抑制PEPCK的基因表达 。 | 袁莉 Reinhard Ziegler Andreas Hamann | 2002 | 中华内科杂志2002，41，10： | 2 |
| 6 | Marine natural products and their potential applications as anti-infective agents显示文摘 | Marwa Donia Mark T Hamann | 2003 | The Lancet Infectious Diseases2003，，6： | 2 |
| 7 | Plant responses to simulated warming and drought:a comparative study of functional plasticity between congeneric mid and high elevation species显示文摘Aims Effects of climate change,especially changes in temperatures and precipitation patterns,are particularly pronounced in alpine regions.In response,plants may exhibit phenotypic plasticity in key functional traits allowing short-term adjustment to novel conditions.However,little is known about the degree of pheno-typic plasticity of high elevation species relative to mid elevation congeners.Methods We transplanted 14 herbaceous perennial species from high eleva-tion into two common gardens(1050 and 2000 m.a.s.l.)in the Swiss Alps,and we examined plastic responses in key functional traits to changes in temperature and soil water availability.This design was replicated with 14 congeneric species from mid elevation to assess if the degree of phenotypic plasticity differs between mid and high elevation species.Survival was assessed across two growing seasons,while aboveground biomass and specific leaf area(SLA)were measured after the first growing season,and biomass alloca-tion to belowground and reproductive structures after the second.Moreover,a phenotypic plasticity index was calculated for the func-tional traits to compare the degree of plasticity between mid and high elevation species.Important Findings Aboveground biomass was higher in mid elevation species relative to high elevation congeners in all treatments,yet decreased for both with elevation and drought.Similarly,SLA decreased with elevation and drought.Root mass fraction(RMF)was generally higher in high ele-vation species,and decreased with drought at the lower site.Drought increased the allocation to reproductive structures,especially when plants were grown at their elevation of origin.Interestingly,no differ-ence was found in the degree of phenotypic plasticity averaged across mid and high elevation species for any of the studied functional traits.These results indicate that phenotypic plasticity in the focal traits did not depend on the elevation of origin of the species.Plasticity was not related to environmental heterogeneity,nor constrained by selective pressures at high elevation.However,both species groups showed a remarkable capacity for short-term acclimation to a prospective cli-mate through rapid adjustments in key functional traits. | Elena Hamann Halil Kesselring Jürg Stöcklin | 2018 | Journal of Plant Ecology2018，11，3： | 2 |
| 8 | Marine pharmacology in 1999 : compounds with antibacterial, anticoagulant, anti fungal, anthelmintic, anti-inflammatory, antiplatelet, anti protozoal and antiviral activities affecting the cardiovascular, endocrine, immune and nervors systems, and other miscella- neous mechanisms of action 显示文摘 | Alejandro M Mayer S Hamann M T | 2002 | Cornp Biochem Physiol Part C2002，132，： | 1 |
| 9 | Candidate gene markers for litter size in different German pig lines显示文摘 | Drogemuller C Hamann H and Distl O | 2001 | Animal Science2001，79，： | 1 |
| 10 | Microvascular basa lamina injury after experimental focal cerebral ischemia and reperfusion in the rat 显示文摘 | HAMANN G F LIEBETRAU M MARTEN H | 2002 | Cereb Blood Flow Metab2002，22，5： | 1 |
| 11 | Integrin αvβ3 is expressed in selected microvessels after focal cerebral ischemia显示文摘 | Okada Y Copeland BR Hamann GF | 1996 | Am J Pathol1996，149，： | 1 |
| 12 | Norm-Conserving Pseudopotentials 显示文摘 | Hamann D R Schlüter M Chiang C | 1979 | Phys Rev Lett1979，43，： | 1 |
| 13 | Phenotypic and functional separation of memory and effector human CD8+ T cells显示文摘 | Hamann D Baars PA Rep MHG | 1997 | J Exp Med1997，186，： | 1 |
| 14 | Erbium: YAG laser-assisted cataract surgery 显示文摘 | Stevens GJr Long B Hamann JM | 1998 | Ophthalmic Surg Lasers1998，29，： | 1 |
| 15 | Leptin stimulates proliferation and TGF β expression in renal glomerular endothelial cells: Potential role in glomeruloselerosis 显示文摘 | Wolf G Hamann A Han D C | 1999 | Kidney Int1999，56，： | 1 |
| 16 | Phenotypic and functional separation of memory and effector human CD8+ T cells显示文摘 | Hamann D Baars P A Rep M H | 1997 | J Exp Med1997，186，9： | 1 |
| 17 | Dynamic CT perfusion imaging of a-cute stroke显示文摘 | Mayer TE Hamann GF Baranczyk J | 2003 | AJNR Am J Neuroradiol2003，21，： | 1 |
| 18 | Distributed, physics-based control of swarms of vehicles 显示文摘 | SPEARS W M SPEARS D F HAMANN J C | 2004 | Autonomous Robots2004，17，2： | 1 |
| 19 | Relationship between instrumental and sensory parameters of cooked sweet potato texture显示文摘 | TRUONG V D WALTER W M HAMANN D D | | 0，，02： | 1 |
| 20 | Development of scale-free climate data for western Canada for use in resource management显示文摘 | Wang T Hamann A Spittlehouse D L | 2006 | International Journal of Climatology2006，26，3： | 1 |
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