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| 1 | Diagnosis of intestinal tuberculosis using a monoclonal antibody to Mycobacterium tuberculosis显示文摘AIM:To investigate the utility of immunohistochemical(IHC) staining with an antibody to Mycobacterium tuberculosis(M.tuberculosis) for the diagnosis of intestinal tuberculosis(TB).METHODS:We retrospectively identified 10 patients(4 males and 6 females;mean age = 65.1 ± 13.6 years) with intestinal TB.Clinical characteristics,including age,gender,underlying disease,and symptoms were obtained.Chest radiograph and laboratory tests,including sputum Ziehl-Neelsen(ZN) staining,M.tuberculosis culture,and sputum polymerase chain reaction(PCR) for tubercle bacilli DNA,as well as Tuberculin skin test(TST) and QuantiFERON-TB gold test(QFT),were examined.Colonoscopic records recorded on the basis of Sato's classification were also reviewed,in addition to data from intestinal biopsies examined for histopathological findings,including hematoxylin and eosin staining,and ZN staining,as well as M.tuberculosis culture,and PCR for tubercle bacilli DNA.For the present study,archived formalin-fixed paraffin-embedded(FFPE) intestinal tissue samples were immunohistochemically stained using a commercially available species-specific monoclonal antibody to the 38-kDa antigen of the M.tuberculosis complex.These sections were also stained with the pan-macrophage marker CD68 antibody.RESULTS:From the clinical data,we found that no patients were immunocompromised,and that the main symptoms were diarrhea and weight loss.Three patients displayed active pulmonary TB,six patients(60%) had a positive TST,and 4 patients(40%) had a positive QFT.Colonoscopic findings revealed that all patients had type 1 findings(linear ulcers in a circumferential arrangement or linear ulcers arranged circumferentially with mucosa showing multiple nodules),all of which were located in the right hemicolon and/or terminal ileum.Seven patients(70%) had concomitant healed lesions in the ileocecal area.No acid-fast bacilli were detected with ZN staining of the intestinal tissue samples,and both M.tuberculosis culture and PCR for tubercle bacilli DNA were negative in all samples.The histopathological data revealed that tuberculous granulomas were present in 4 cases(40%).IHC staining in archived FFPE samples with anti-M.tuberculosis monoclonal antibody revealed positive findings in 4 patients(40%);the same patients in which granulomas were detected by hematoxylin and eosin staining.M.tuberculosis antigens were found to be mostly intracellular,granular in pattern,and primarily located in the CD68 + macrophages of the granulomas.CONCLUSION:IHC staining with a monoclonal antibody to M.tuberculosis may be an efficient and simple diagnostic tool in addition to classic examination methods for the diagnosis of intestinal TB. | Yasushi Ihama Akira Hokama Kenji Hibiya Kazuto Kishimoto Manabu Nakamoto Tetsuo Hirata Nagisa Kinjo Haley L Cash Futoshi Higa Masao Tateyama Fukunori Kinjo Jiro Fujita | 2012 | World Journal of Gastroenterology2012，18，47： | 12 |
| 2 | KIT mutations are common in incidental gastrointestinal stromal tumors one centimeter or less in size显示文摘 | Corless CL McGreevey L Haley A | 2002 | Am J Pathol2002，160，5： | 1 |
| 3 | Multiple marker mapping of quantitative trait loci in a cross between outbred wild boar and large white pigs显示文摘 | Knott S A Marklund L Haley C S | | 0，，： | 1 |
| 4 | Genetics of larval tolerance toreduced salinities in two populations of oysters,Crassostreavirginica显示文摘 | Newkirk G F Waugh D L Haley L E | 1977 | Journal of the Fisheries Research Board of Canada1977，34，3： | 1 |
| 5 | The PiGMaP consor- tium linkage map of the pig (Sus scrofa)显示文摘 | Archibald A L Haley C S Brown I F | 1995 | Mammalian Ge- nome1995，6，3： | 1 |
| 6 | Genetic mapping of quantitative trait loci for growth and fatness in pigs 显示文摘 | Andersson L Haley C S Ellegren H | 1994 | Science1994，263，25： | 1 |
| 7 | Genetics of larvae and spat growth rate in the oyster Crassostrea virginica 显示文摘 | Newkirk G F Haley L E Waugh D L | 1977 | Marine Biology1977，41，： | 1 |
| 8 | Genetics of lavae and spat growth rates in the oyster, Crassostrea virginica显示文摘 | NEWKIRK G F HALEY L E WAUGH D L | 1977 | Mar Biol1977，41，： | 1 |
| 9 | Antioxidant properties of hard winter wheat extracts显示文摘 | Yu L Haley S Perret J | 2002 | Food chemistry2002，78，： | 1 |
| 10 | Genetic mapping of quantitative trait loci for growth and fatness in pigs显示文摘 | Andersson L Haley C S Ellegren H | | 0，，： | 1 |
| 11 | A polymor phism in the 5'-Untranslated region of the porcine cholecys- tokinin type A receptor gene affects feed in take and growth 显示文摘 | Houston R D Haley C S Archibald A L | 2006 | Genetics Sociaety of America2006，10，： | 1 |
| 12 | Selecting oysters for faster growth显示文摘 | Haley L E Newkirk G F | 1977 | Proc World Maricul Soce1977，，8： | 1 |
| 13 | Late fall fertilization of Kentucky bluegrass显示文摘 | Wehner D J Haley J E Martin D L | 1998 | Agron J1998，88，： | 1 |
| 14 | Genetics of larvae and spat growth rate in the oyster Crassostrea virginica 显示文摘 | Newkirk G F Haley L E Waugh D L | 1977 | Marine Biology1977，41，： | 1 |
| 15 | Genetics of larval tolerance to reduced salinities in two populations of oysters, Crassostrea virginica 显示文摘 | Newkirk G F Waugh D L Haley E | 1977 | J Fish Res Board Can1977，34，： | 1 |
| 16 | Later fall fertilization of Kentucky bluegrass显示文摘 | Wehner D J Haley J E Martin D L | 1988 | Agronomy Journal1988，80，： | 1 |
| 17 | Growth rate and survival in pure population matings and crosses of the oyster Crassostrea virginica 显示文摘 | Mallet A L Haley L E | 1983 | Can J Fish Aquat Sci1983，40，： | 1 |
| 18 | Histopathology of in-stent restenosi in patients with peripheral artery disease显示文摘 | Kearney M Pieczek A Haley L | 1997 | Circulation1997，95，8： | 1 |
| 19 | The effect of network formation on the rheological properties of SBR modified asphalt binders显示文摘 | Lee Y J France L M and Haley M C | 1997 | Rubber Chem & Technol1997，70，： | 1 |
| 20 | Genetics of larval tolerance to reduced salinities in two populations of oysters,Crassostrea virginica显示文摘 | Newkirk G F Waugh D L Haley L E | 1977 | Journal of the Fisheries Board of Canada1977，34，3： | 1 |
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