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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | 多种形式促动医患协作 营造自下而上的患者安全文化显示文摘通过对国内外医患关系和患者安全文化的现状进行梳理,明晰推广患者安全文化的必要性和紧迫性,着重介绍澳大利亚新南威尔士州为促进患者安全发起的患者合作、临床领导力改善等重点项目,以期为我国创建患者安全文化拓宽思路,提供借鉴和启示。Promote patient partnering and the bottom-to-top patient safety culture in various forms/SUN Dongyue,SUN Niuyun,Clifford F Hughes,et al//Chinese Hospitals.-2012,16(10) | 孙冬悦 孙纽云 Clifford F Hughes Peter Kennedy | 2012 | 中国医院2012，16，10： | 3 |
| 3 | 逐步推动政府主导、多方协作的患者安全管理保障体系建设显示文摘在系统介绍澳大利亚新南威尔士州卫生行政部门机构沿革和职能转变的基础上,着重从法律法规建立、法定卫生组织支撑、支付筹资手段改革、信息系统设计、专项措施和评估手段、安全文化建设等6个方面分析了其患者安全与质量管理体系的构建过程,为我国患者安全和质量管理体系建立和完善提供参考和建议。 | 孙纽云 房珊杉 Clifford F Hughes Peter Kennedy | 2012 | 中国医院2012，16，10： | 2 |
| 4 | On drilling degrees of freedom显示文摘 | Hughes T J R Brezzi F | 1989 | Computer Methods in Applied Mechanics and Engineering1989，72，： | 2 |
| 5 | ST2可能并非心血管事件、心力衰竭和死亡有用的预测因子显示文摘研究人员假设可溶性ST2(soluble ST2,sST2)水平可识别出心血管疾病(cardiovascular disease,CVD)风险较高的人群,应将其纳入风险预测计算指标。ST2是炎症性细胞因子白细胞介素33的受体。sST2水平增加与急性心肌梗死患者和一般人群的心力衰竭及死亡相关。方法:研究人员测定FINRISK97前瞻性研究人群队列中的8444名男性和女性(25-74岁)的高敏sST2水平,应用Cox比例风险模型评估在15年的随访中,sST2对致命性或非致命性心力衰竭、CVD(冠状动脉性心脏病和脑卒中)、糖尿病和死亡情况的预测能力。研究者获取10年绝对风险识别和重新分类的统计数据, | 赵狄 叶鹏 Hughes MF Appelbaum S Havulinna AS Jagodzinski A Zeller T2 Kee F Blankenberg S Salomaa V | 2014 | 中华高血压杂志2014，22，8： | 2 |
| 6 | Computational of tailing-edge noise due to turbulent flow over an airfoil 显示文摘 | OBERAI A RONALDKIN F HUGHS T | 2002 | AIAA Journal2002，40，5： | 1 |
| 7 | C/EBPα and HNF6 protein complex formation stimulates HNF6-dependent transcription by CBP coactivator recruitment in HepG2 cells显示文摘 | HUGHES D E RAUSA F M | 2006 | Hepatology2006，43，2： | 1 |
| 8 | Elastic tripping analysis of asymmetrical stiffeners 显示文摘 | Ma M | 1996 | Computers & Structures1996，60，3： | 1 |
| 9 | Climate in Medieval time显示文摘 | Bradley R S Hughes M K Diaz H F | 2003 | Science2003，302，： | 1 |
| 10 | Large-scale prediction of Saccharomyces cerevisiae gene function using overlapping transcriptional clusters显示文摘 | Wu L F Hughes T R Davierwala A P | 2002 | Nat Genet2002，31，7： | 1 |
| 11 | Turbulent two-phase flow in faceshaft seals 显示文摘 | Beatty P A Hughes W F | 1986 | ASLE Transactions1986，109，1： | 1 |
| 12 | A new method for the nonlinear transformation of means and covariances in filters and estimators 显示文摘 | SIMON JULIER JEFFREY UHLMANN HUGH F DURRANT-WHYTE | 2000 | IEEE Transactions on Automatic Control2000，45，3： | 1 |
| 13 | The hepatitis B virus core and e antigens elicit different Th cell subsets: antigen structure can affect Th cell phenotype 显示文摘 | Milich DR SchodeI F Hughes JL | 1997 | J Virol1997，71，21： | 1 |
| 14 | Adverse events and near miss reporting in the NHS显示文摘 | Shaw R Drever F Hughes H | 2005 | Qual Saf Health Care2005，14，： | 1 |
| 15 | Photochemical decomposition of hydrazobenzene, studied by the use of nitrogen isotopes 显示文摘 | Holt P F Hughes B P | 1955 | J Chem Soc1955，，： | 1 |
| 16 | Flame Extin- guishment Properties of Dry Chemicals: Extinction Concen- trations for Small Diffusion Pan Fires 显示文摘 | EWING C T FAITH F R HUGHES J T | 1989 | Fire Technology1989，，5： | 1 |
| 17 | Biochemical identification of apoptosis(programmed cell death) in granulosa cells: evidence for a potential mechanism underlying follicular atresia显示文摘 | HUGHES F M J R GOROSPE W C | 1991 | Endocrinology1991，129，5： | 1 |
| 18 | Inelastic analysis of panel collapse by stiffener buckling 显示文摘 | Ma M | 1996 | Computers & Structures1996，61，1： | 1 |
| 19 | A prokaryotic phytochrome显示文摘 | Hughes J Lamparter T Mittmann F | 1997 | Nature1997，386，： | 1 |
| 20 | In situ three-dimensionalcharacterization of membrane fouling by protein suspensions usingmultiphoton microscopy显示文摘 | Hughes D J Cui Z F Field R W | 2006 | Langmuir2006，22，14： | 1 |
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