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| 1 | Cloning and characterization of the Thcutl gene encoding a cutinase of Trichoderma harzianum T34 显示文摘 | RUBIO M B CARDOZA R E HERMOSA R | 2008 | Curr Genet2008，54，： | 1 |
| 2 | The contribution of Trichoderma to balancing the costs of plant growth and defense显示文摘 | HERMOSA R RUBIO M B CARDOZA R E | 2013 | International Microbiology2013，16，2： | 1 |
| 3 | Development of a strain-specific SCAR marker for the detection of Trichoderma atroviride 11,a biological control agent against soilborne fungal plant pathogens显示文摘 | Grondona I Minguez J M D | 2001 | Current Genetics2001，38，6： | 1 |
| 4 | Molecular characterization and identification of biocontrol isolates of Trichoderma spp显示文摘 | Hermosa M R Grondona I Iturriaga E A t al | 2000 | Applied and environmental microbiology2000，66，5： | 1 |
| 5 | Physiological and biochemical characterization of Trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens显示文摘 | Grondona I Hermosa R Tejada M t al | 1997 | Applied and environmental microbiology1997，68，： | 1 |
| 6 | Overex- pression of a Trichoderma HSP 70 gene increases fungal resist- ance to heat and other abiotic stresses 显示文摘 | MonterO-tMrrientos M Hermosa R Nieold C et aI | 2008 | Fungal Genetics and Biology2008，45，11： | 1 |
| 7 | The contribution of Trichoderma to balancing the costs of plant growth and defense 显示文摘 | HERMOSA R RUBIO M B CARDOZA R E | 2013 | International Microbiology2013，16，2： | 1 |
| 8 | Overexpression of a Trichoderma HSP70 gene increases fungal resistance to heat and other abiotic stresses 显示文摘 | Montero-Barrientos M Hermosa R Nicolas C | 2008 | Fungal Genetics and Biology2008，45，11： | 1 |
| 9 | Development of a strain-specific SCAR marker for the detection of Trichoderma atroviride 11,a biological control agent against soilborne fungal plant pathogens显示文摘 | Hermosa M R Grondona I Diaz-Minguez J M | 2001 | Curr Genet2001，38，： | 1 |
| 10 | Development of a strain- specific SCAR marker for the detection of Trichoderma atroviride 11, a biological control agent against soilborne fungal plant pathogens显示文摘 | HERMOSA M R GRONDONA I MINGUEZ J M D | 2001 | Current Genetics2001，38，6： | 1 |
| 11 | Transgenic expression of the Trichoderma harzianum hspT0 gene increases Arabidopsis resistance to heat and other abiotic stresses显示文摘 | Montero-Barrientos M Hermosa R Cardoza R E | 2010 | Plant Physiol2010，167，： | 1 |
| 12 | Identification and characterization of potato protease inhibitors able to inhibit pathogenicity and growth of botrytis cinerea 显示文摘 | HERMOSA M R TURRà D FOGLIANO V | 2006 | Physiological and Molecular Plant Pathology2006，68，46： | 1 |
| 13 | Identification of loci and functional characterization of trichothecene biosynthesis genes in filamentous fungi of the genus Trichoderma显示文摘 | Cardoza R E Malmierca M G Hermosa M R | 2011 | Applied and Environmental Microbiology2011，77，14： | 1 |
| 14 | Transgenic expression of the Trichoderma harzi- anum hsp70 gene increases Arabidopsis resistance to heat and other ahiotie stresses显示文摘 | MONTERO-BARRIENTOS M HERMOSA R CARDOZA R E | 2010 | Journal of Plant Physiology2010，167，8： | 1 |
| 15 | Thctfl Transcription Factor of Trichoderma harzianum Is Involved in 6-Pentyl-2H-pyran-2-one Production and Antiftmgal Activity显示文摘 | RUBIO M B HERMOSA R REINO J L | 2009 | Fungal Genetics and Biology2009，46，1： | 1 |
| 16 | Physiological and biochemical characterization of Trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens显示文摘 | GRONDONA I HERMOSA R TEJADA M | 1997 | Applied and Environmental Microbiology1997，63，8： | 1 |
| 17 | Cloningand characterization of the thcutl gene encoding a cutinase ofTrichoderma harzianum T34显示文摘 | RUBIO M B CARDOZA R E HERMOSA R | 2008 | Curr Genet2008，54，6： | 1 |
| 18 | Functional genomic approach to the study of biodiversitywithin Trichoderma显示文摘Trichoderma is a fungal genus of great and demonstrable biotechnological value, but its genome is poorly surveyed compared with other model microorganisms. Due to their ubiquity and rapid substrate colonization, Trichoderma species have been widely used as biocontrol organisms for agriculture, and their enzyme systems are widely used in industry. Therefore, there is a clear interest to explore beyond the phenotype to exploit the underlying genetic systems using functional genomics tools. The great diversity of species within the Trichoderma genus, the absence of optimized systems for its exploration, and the great variety of genes expressed under a wide range of ambient conditions are the main challenges to consider when starting a comprehensive functional genomics study. An initial project started by three Spanish groups has been extended into the project TRICHOEST, funded by the EU (FP5, QLRT-2001-02032) to target the transcriptome analysis of selected Trichoderma strains with biocontrol potential, in conditions related to antagonism, nutrient stress and plant interactions. Once specific conditions were defined, cDNA libraries were produced and used for EST sequencing. Nine strains from seven Trichoderma species have been considered in this study and an important amount of gene sequence data has been generated, analyzed and used to compare the gene expression in different strains. In parallel to sequencing, genomic expression studies were carried out by means of macro-arrays to identify genes expressed in specific conditions. In silico analysis of DNA sequencing data together with macro-array expression results have lead to a selection based on the potential use of the gene sequences. The selected clone sequences were completed and cloned in appropriate vectors to initiate functional analysis by means of expression studies in homologous and heterologous systems. | Monte E Hermosa M R González F J Rey M Cardoza R E Gutiérrez S Delgado Jarana J Llobell A | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 1 |
| 19 | Cell wall degrading isoenzyme profiles of Trichoderma biocontrol strains show correlation with rDNA species显示文摘Species of the fungus Trichoderma, a genus of Hyphomycetes, are ubiquitous in the environment, but especially in soil. They have been used in a wide range of commercial applications including the production of hydrolases and in the biological control of plant diseases. A fundamental part of the Trichoderma antifungal system consists of a series of genes coding for a surprising variety of extracellular cell wall degrading enzymes (CWDE). Characterisation and identification of strains at the species level is the first step in utilizing the full potential of fungi in specific applications. One aim when isolating Trichoderma strains is to identify those which can be used in new agricultural and industrial applications. In the past it was not uncommon that biocontrol strains were defined as T. harzianum Rifai, due to the limited classification system of the genus Trichoderma. In recent years, several PCR-based molecular techniques have been used to detect and discriminate among microorganisms. Sequence analysis of the ITS regions of the ribosomal DNA and gene fragments as those corresponding to tef1 gene have been helpful in the neotypification, description and characterization of species in the genus Trichoderma. Another useful method for the identification of Trichoderma strains is the randomly amplified polymorphic DNA (RAPD) technique. Isozyme polymorphisms evaluation of five putative extracellular lytic enzymes loci (β-1,3-glucanase, β-1,6-glucanase, cellulase, chitinase and protease antivities) were carried out using representative strains of defined molecular groups. CWDE groupings obtained from biocontrol strains are discussed in relation to their phylogenetic location and antifungal activities. Compiling morphological, biochemical and sequence information data into a common database would provide a useful resource that could be used to accurately name new haplotypes identified in the future and correctly place them within the genus Trichoderma. | Sanz L Hermosa M R González F J Monte E | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 0 |
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