| 1 | DC dielectrophoresis separation of marine algae and particles in a microfluidic chip显示文摘This paper reports a microfluidic method of continuous separation of marine algae and particles by DC dielectrophoresis. The locally non-uniform electric field is generated by an insulating PDMS triangle hurdle fabricated within a PDMS microchannel. Both the particles and algae are subject to negative DEP forces at the hurdle where the gradient of local electric-field strength is the strongest. The DEP force acting on the particle or the algae depends on particles' or algae's volume, shape and dielectric properties. Thus the moving particles and algae will be repelled to different streamlines when passing the hurdle. In this way, combined with the electroosmotic flow, continuous separation of algae of two different sizes, and continuous separation of polystyrene particles and algae with similar volume but different shape were achieved. This first demonstration of DC DEP separation of polystyrene particles and algae with similar sizes illustrates the great influence of dielectric properties on particle separation and potentials for sample pretreatment. | SONG YongXin1, YANG JianDong1, SHI XiaoFei4, JIANG Hai3, WU YanBin1, PENG Ran1, WANG Qi5, GONG Ning2, PAN XinXiang1, SUN YeQing2 & LI DongQing1,31Department of Marine Engineering, Dalian Maritime University, Dalian 116026, China 2Institute of Environmental Systems Biology, Dalian Maritime University, Dalian 116026, China 3Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, ON, N2L 3G1, Canada 4College of Information Science and Technology, Dalian Maritime University, Dalian 116026, China 5Department of Respiratory Medicine, the Second Hospital Affiliated to Dalian Medical University, Dalian 116026, China | 2012 | Science China Chemistry2012，55，4： | 3 |
| 3 | Molecular Cloning of a Cytosolic G6PDH Gene from Populus Suaveolens and Its Expression to Improve the Cold Resistance of Tobacco Plants显示文摘Glucose-6-phosphate dehydrogenase (G6PDH,EC 1.1.1.49) is the first and main regulated enzyme of oxidative pentose phosphate pathway (OPPP),catalyzing the conversion of glucose-6-phosphate to 6-phospho-gluconolactone and playing important roles in the growth and development of plants. It is preciously reported that the enhancement of freezing resistance of Populus suaveolenscuttings is clear related to the distinct increase in cytosolic G6PDH activity. Here,a 1697 bp cDNA fragment (PsG6PDH) is amplified by RT-PCR from cold-induced total RNA of the freezing-tolerant P. suaveolens. A sequence analysis showed that PsG6PDH coding region had 1 530 bp and encoded 510 predicted amino acid residues. Genomic Southern analysis revealed that the isoform is encoded by a few copies of the gene in the poplar genome. The cloned gene PsG6PDHis cloned into binary vector pBI121 and used to transform tobacco. PCR and Southern blotting results verified integration of this gene into the genome of tobacco. Moreover,cold treatment experiments and membrane defense enzymeactivity analysis confirmed that overexpression of the PsG6PDHgene could enhance the tolerance to cold or frigid stresses in transgenic plants. | LIN Yuan-zhen1,2,GUO Hai3,ZHANG Zhi-yi2,LIN Shan-zhi2 (1. College of Forestry,South China Agricultural University,Guangzhou Guangdong 510642,China 2. Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,MOE,Beijing Forestry University,Beijing 100083,China 3. Plant Materials Center for Soil and Water Conservation,Ministry of Water Resources,Beijing100038,China) | 2009 | 冰川冻土2009，31，6： | 0 |
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