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您的检索式：作者名="H.M.Liebich"
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| 1 | 细胞中鸟苷二磷酸糖的毛细管电泳研究显示文摘葡萄糖是细胞生长和代谢的一种重要的调节剂 .鸟苷二磷酸糖 (UDP Sug ars)作为其代谢的中间产物 ,在碳水化合物、糖类酯等的合成中起着重要作用 .研究其在细胞中的代谢在临床生物化学中具有重要意义 .建立了一个毛细管电泳方法 ,采用 70cm× 5 0 μm的未涂毛细管 ,2 0mmol/L硼酸盐 (pH9)作缓冲液 ,在 2 2kV下 ,可在15min左右全分离 4个关键UDP Sugars,精度和检测限满足细胞分析要求 .经实际分析淋巴细胞、成纤维细胞和肾小球细胞 ,表明此方法不仅比HPLC具有明显优点 。 | 许国旺 R.Lehmann 路鑫 H.M.Liebich 张玉奎 卢佩章 | 1999 | 中国科学(B辑)1999，29，2： | 2 |
| 2 | Capillary electrophoretic study on UDP-sugars in cells显示文摘Glucose is an important regulator of cell growth and metabolism. Uridine diphosphate sugars (UDP-sugars), as the intermediate products of metabolism, play pivotal roles as precursors in the synthesis of complex carbohydrates and glycolipids as well as lectose. It is very important to study their metabolism in cells in clinical biochemistry. A capillary electrophoretic method has been developed for the analysis of UDP-sugars and nucleotides. By using an uncoated capillary (70cm×50μm) and 20 mmol/L borax buffer (pH 9), 4 important UDP-sugars can be analyzed in 15 min at 22 kV with satisfactory precision and sensitivity. The developed method has been applied to analyze UDP-sugars concentrations in lymphocytes, fibroblasts and mesangial cells, and the results show it not only is much better than HPLC method, but also can be used to measure the energy charge of cells. | 许国旺 R.Lehmann 路鑫 H.M.Liebich 张玉奎 卢佩章 | 2000 | Science China Chemistry2000，43，1： | 0 |
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