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| 1 | Chemical Composition and Antimicrobial Activity of Essential Oil of Asarum caulescene显示文摘Both essential oils of fresh and air-dried Asarum caulescene (Maxim.) were analyzed by GC-MS in three different polarities. The results show that thirty-four components were identified in the oils, and main compounds of both oils were β-pinene (7.63% vs. 20.72%), 2-carene (8.94% vs. 7.99%), β-phellandrene (7.80% vs. 14.39%), 1,8-cineole (8.91% vs. 13.29%) and germacrone (46.64% vs. 6.64% ), however, the content of monoterpene hydrocarbons(30.29% vs. 52.30% ) and oxygenated sesquiterpenes( 47.69% vs. 10.65%) of both oils was different. The antimicrobial activity of the oil was evaluated against 3 fungi and 11 bacteria including 7 clinical isolated strains. Our results showed that both oils presented a broad antimicrobial spectrum, and compared with the essential oil from fresh herb, the extracted oil from dried herb had better antimicrobial activity, its minimum inhibitory concentration(c_ mi ) values of dried herb were 0.16-2.5 g/L and lowest minimum bactericidal concentration(c_ mb ) value was 0.31 g/L. | ZHU Shunying YANG Yang YU Huaidong YING Yue LONG Dong Zou Guolin | 2006 | Wuhan University Journal of Natural Sciences2006，11，3： | 7 |
| 2 | Thermostability of Subtilisin Nattokinase Obtained by Site-Directed Mutagenesis显示文摘To study the thermostability of Nattokinase(subtilisin NAT,NK),three double mutant plasmids(pET-28a-NKG61C/S98C,pET-28a-NKT22C/S87C,pET-28a-NKS24C/S87C)were constructed by site-directed mutagenesis.Target enzymes were detected using SDS-PAGE and disulfide bond formation was detected using Western blotting analysis.Thermostability was tested by rates of inactivation at certain temperature.The results showed that disulfide bond was not formed within two cysteines and the thermostability of three double mutants was not increased compared with the wild-type NK.The thermostability of NK performed in Ca2+was stronger than in ethylenediaminetetraacetic acid(EDTA).But when the temperature reached 62℃,the enzymes rapidly denatured and inactivated even in the presence of Ca2+.Although the thermostability of mutants was not increased,this study shows a tendency of improving thermostability of NK in protein engineering. | WENG Meizhi DENG Xiongwei WU Jieyuan ZOU Guolin | 2014 | Wuhan University Journal of Natural Sciences2014，19，3： | 6 |
| 3 | Purification and Characterization of an Antibacterial Protein from the Cultured Mycelia of Cordyceps sinensis显示文摘抗菌剂蛋白质从 Cordycepssinensi.f 的有教养的 mycelia 被孤立,并且被指定为 Cordyceps sinensis 抗菌剂蛋白质(CSAP ) 。CSAP 锁住 wassingle,与 35 x 10 ~ 的一个明显的分子的团, 3 由 SDS 页和新奇恐水病的 N 终端顺序 N-ALATQHGAP 揭示了。抗菌剂试金证明 CSAP 能对真菌或酵母禁止克积极、克否定的细菌而是没有重要抑制的生长。而且, CSAP 的抗菌剂活动不杀菌却细菌抑制。抗菌剂蛋白质在 Cordyceps 种类被描述的 Itwas 第一次,它可能在主人的化学防卫机制包含。 | HU Zheng YE Maoqing XIA Liqiu TU Wenjuan LI Liang ZOU Guolin | 2006 | Wuhan University Journal of Natural Sciences2006，11，3： | 5 |
| 4 | Four residues of propeptide are essential for precursor folding of nattokinase显示文摘Subtilisin propeptide 作为指导先锋合拢的 intramolecular 女伴工作。Nattokinase, subtilisin 家庭的一个成员,当由信号肽, propeptide,和一个 subtilisin 领域组成的一位先锋,和它的合拢的机制尚待被理解,被综合。在这研究,贡献先锋合拢的 nattokinase propeptide 的必要残余是坚定的。删除分析证明在 propeptide 的保存区域为先锋合拢是重要的。单个地点并且多地点 mutagenesis 研究证实了 Tyr10, Gly13, Gly34,和 Gly35 的角色。在期间上演(i) 并且(ii ) 将先锋合拢, Tyr10 和 Gly13 形成 subtilisin 的接口的部分域。当 Gly34 和 Gly35 与将稳定 propeptide 的结构的一个螺旋连接了时。四倍的翼变化, Y10A/G13A/G34A/G35A,为 propeptide 导致了女伴功能的损失。这个工作为先锋经由第二等的结构和运动参数分析合拢显示出 propeptide 的必要残余。 | Yan Jia Xinhua Cao Yu Deng Wei Bao Changyan Tang Hanjing Ding Zhongliang Zheng Guolin Zou | 2014 | Acta Biochimica et Biophysica Sinica2014，46，11： | 4 |
| 5 | Studies on the Preparation and the Stability of SOD Modified by Lauric Acid显示文摘Tkls paper reported that a way or Preparlng superoxlde dlsmutase (SoD) modiried by lauric acid(LA-SoD) and study on stablllty of LA-SoD. The resultS showed that LA-SoD had high speclfic actlvlty,highstablllty and high rate of recovery. The stabiliy or LA-SoD ln temperature, pH and kuman gastrlc juice wasmuch higher than that of natural SOD. Because LA-SOD ls kept for a long tlme at room temperature, it canwiden the appIled range of SoD and brlng out conslderable benirlclaI results both in society and economy. | Gui Xingfeu, Yang Ping, Qiu Yanxia, Liu Jun(Henan Medical College for Health Staff, Zhengzhou 450003,China)Zou Guolin(College of Life Sciences,Wuhan University, Wuhan 430072,China) | 1998 | Wuhan University Journal of Natural Sciences1998，3，2： | 2 |
| 6 | Binding of Puerarin to Human Serum Albumin: A Spectroscopic Analysis and Molecular Docking显示文摘 | Yang He Yiwei Wang Lifei Tang Hui Liu Wei Chen Zhongliang Zheng Guolin Zou | 2008 | Journal of Fluorescence2008，，2： | 1 |
| 7 | Antibacterial activities of nine Chinese medicinal plants显示文摘 | Hou Jie Yin Yan Zou Guolin | 2009 | Journal of Wuhan University2009，55，5： | 1 |
| 8 | Chemical composition and antimicrobial activity of the essential oil of Scutellaria barbata显示文摘 | Jianqing Yu Jiachuan Lei Huaidong Yu Xuan Cai Guolin Zou | 2004 | Phytochemistry2004，，7： | 1 |
| 9 | Application of an Optical Biosensor to Study the Interaction between Porphyrins and Wheat Germ Agglutinin显示文摘有一个搅动的透明小容器的一个光生物传感器被用来带着二只水溶性的猫监视使不能调动的小麦细菌凝集素(WGA ) 的相互作用免费基础卟啉和它的 Zn (II ) 的离子的 por-phyrins.The 协会常数(K_a ) 复杂形式 2。第 66a 27.31 x 10 ~ 在 20 C 的 5 L/mol 分别地。免费基础卟啉的相互作用进一步在在 15 C 和 37 C 之间的温度被调查。热力学参数,在免费精力的变化,热含量和熵,—31.23, 22.92, 54.15 kJ/mol 分别地。热能力变化是—355.53 J 中心点 mol~(-1) 中心点 K~(-1) 。绑定是驾驶 byentropic 贡献,并且显示出强壮的热含量熵赔偿。它首先被恐水病的力量管理。 | QIN Yimin PAN Nina ZOU Guolin | 2006 | Wuhan University Journal of Natural Sciences2006，11，2： | 0 |
| 10 | Chemical composition and antimicrobial activity of the essential oil of Cacalia tangutica (Maxim.) Hand.-Mazz显示文摘Essential oil of the subterranean part of Cacalia tangutica(Maxim.)Hand.-Mazz was analyzed by gas chromatography(GC)-mass spectrum(MS)technique in two different capillary columns of different polarities.Thirty-one components were identified in the oil and the main compounds were a-zingiberene(13.49%),germacrene D(10.76%),α-pinene(8.54%),caryophyllene(Z-)(6.36%),linalool(6.16%),β-myrcene(4.89%),β-ocimene(Z-)(4.40%)and ocimenone(Z-)(3.58%).The antimicrobial activity of the oil was evaluated against 2 fungi and 12 bacteria including 6 clinically isolated strains using the agar disc diffusion and broth microdilution methods.The results show that the oil presented a broad antimicrobial spectrum and had better antimicrobial activity against yeast and gram-positive bacteria.The minimum inhibitory concentration values were 0.16–5.00 g/L and minimum bactericidal concentration values were 0.16–5.00 g/L. | Yang YANG Shunying ZHU Xiaoqiang CAI Lifei TANG Deqing LIU Jinghua HUANG Guolin ZOU | 2008 | Frontiers in Biology2008，3，4： | 0 |
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