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| 1 | Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface显示文摘A new cell membrane preparation, silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the adsorption. The surface characteristics of the silica CCM have been tested by a scanning electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes. The interactions between calcium antagonists and rabbit red and cardiac muscle cell membranes, and the stereoscopic interactions between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system would | Langchong He Guangde Yang Xindu Geng | 1999 | Chinese Science Bulletin1999，44，9： | 58 |
| 2 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 3 | Leaf gas exchange and photosynthesis curves of Elymus nutans and Potentilla anserina under fencing and grazing conditions in the Qilian Mountains, Northwest China显示文摘Potentilla anserina L.and Elymus nutans Griseb.are dominant species in the subalpine meadows of China.Grazing is one of the most important factors that influence community structure and productivity of subalpine meadows.Understanding how grazing changes photosynthetic capability is essential for preservation and restoration of grasslands.However,information about the effects of grazing on photosynthetic capability remains inadequate.Experiments were conducted in fencing and grazing areas in the Qilian Mountains,Northwest China.The leaf gas exchange and photosynthetic curves of P.anserina and E.nutans were measured at different growth stages.Results showed that grazing decreased the values of leaf gas exchange parameters,such as net photosynthetic rate,stomatal conductance,transpiration rate,and intercellular CO2 concentration of P.anserina and E.nutans.In addition,grazing decreased the values of net photosynthetic rate-photosynthetically active radiation(PN-PAR)curve parameters,such as light-saturated net photosynthetic rate,apparent quantum efficiency,light compensation point,light saturation point,and dark respiration rate.Our results demonstrated that grazing was the primary limiting factor for photosynthesis of dominant grassland species in the study area. | LIU Junjie WANG Xiaoping GAO Yunfei RONG Zhanlei ZHANG Guangde WANG Wenbin GE Lijuan MAO Yahua GUO Zhaoxia ZHAO Chuanyan | 2019 | Journal of Arid Land2019，11，3： | 3 |
| 4 | Potential recoverable natural gas resources in China显示文摘在中国的天然气资源是丰富的。在中国的未被发现的可重获的天然气资源被估计是 19.27 敳瀠獯瑩吗? | Liu Chenglin Zhu Jie Che Changbo Liu Guangd | 2008 | Petroleum Science2008，5，1： | 3 |
| 5 | Extinction of the zero-phonon line and the first-order phonon sideband in excitonic luminescence of ZnO at room temperature: the self-absorption effect显示文摘It is firmly demonstrated in experiment that the self-absorption(SA) effect can lead to the extinction of the zero-phonon line and the first-order longitudinal optical phonon sideband of free excitonic luminescence of ZnO at room temperature. Moreover, effectiveness degree of SA effect is found to be dependent on both absorption coefficient and travelling distance of emitted photons, as well as even lattice temperature, which is uniquely reflected by the redshift amount in emission peak in ZnO. It is also unambiguously proved that the SA effect still strictly obeys the Beer-Lambert law of absorption. This work not only uncovers the long-term puzzle of significant redshift of emission peak of ZnO at higher temperatures, but also shows that the SA effect may have to be carefully taken into consideration in the study of spontaneous emission, laser and relevant optoelectronic processes in luminescent materials and optoelectronic devices. | Honggang Ye Zhicheng Su Fei Tang Changcheng Zheng Guangde Chen Jian Wang Shijie Xu | 2017 | Science Bulletin2017，62，22： | 2 |
| 6 | Development of the model of pancreatic β-cell membrane chromatography and its chromatographic characteristics显示文摘A new model of pancreatic β-cell membrane chromatography has been established by using a β-cell membrane stationary phase (β-CMSP) prepared by immobi-lizing the β-cell membrane onto the surface of silica carrier. The protein level and K+, Na+-ATP enzymatic activity of the β-CMSP were detected respectively. The surface characteris-tics of the β-CMSP were tested by using the scanning elec-tron microscope and surface energy spectrometer. In this model, the column (10 mm × 2 mm, I.D.) packed with β-CMSP, 25 mmo1/L ammonium sulfate buffer solution (pH 7.4) as mobile phase with the flow rate of 0.2 mL/min at 37 ± 0.5℃ were used in the following studies. The retention char-acteristics of the sulfonylureas (gliquidone, glibenclamide, gliclazide and glipizide) were investigated under the chro-matographic conditions above. The affinities of the sulfony-lureas on β-cell membrane and receptor will be expressed by using the logk′ values (the logarithm of capacity factor of a solute) in the model. The correlation of the affinity with the pharmacological effect of the sulfonylureas was analyzed also. | YANG Guangde HE Langchong BIAN Xiaoli ZHAO Liang | 2005 | Chinese Science Bulletin2005，50，21： | 2 |
| 7 | Research of the effect of the application of reducing egg-producing chicken's feed use everyday by adding enzyme preparation 显示文摘 | Jian He Guangde feng | 2001 | Food and Feed industry2001，，9： | 1 |
| 8 | Redesign of gravity support system for ITER construction 显示文摘 | Li Pengyuan Hou Binglin Jian Guangde | 2010 | Fusion Engineering and Design2010，85，： | 1 |
| 9 | Modified non-rectangular hyperbola equation with plant height for photosynthetic light-response curves of Potentilla anserina and Elymus nutans at various growth phases in the Heihe River Basin, Northwest China显示文摘The non-rectangular hyperbola(NRH)equation is the most popular method that plots the photosynthetic light-response(PLR)curve and helps to identify plant photosynthetic capability.However,the PLR curve can't be plotted well by the NRH equation at different plant growth phases due to the variations of plant development.Recently,plant physiological parameters have been considered into the NRH equation to establish the modified NRH equation,but plant height(H),an important parameter in plant growth phases,is not taken into account.In this study,H was incorporated into the NRH equation to establish the modified NRH equation,which could be used to estimate photosynthetic capability of herbage at different growth phases.To explore photosynthetic capability of herbage,we selected the dominant herbage species Potentilla anserina L.and Elymus nutans Griseb.in the Heihe River Basin,Northwest China as the research materials.Totally,twenty-four PLR curves and H at different growth phases were measured during the growing season in 2016.Results showed that the maximum net photosynthetic rate and the initial slope of PLR curve linearly increased with H.The modified NRH equation,which is established by introducing H and an H-based adjustment factor into the NRH equation,described better the PLR curves of P.anserina and E.nutans than the original ones.The results may provide an effective method to estimate the net primary productivity of grasslands in the study area. | LIU Junjie WANG Xiaoping RONG Zhanlei GAO Yunfei ZHANG Guangde WANGWenbin GE Lijuan MAO Yahua GUO Zhaoxia WANG Qingtao ZHAO Chuanyan | 2019 | Journal of Arid Land2019，11，5： | 1 |
| 10 | 显示文摘 | Chen Guangde Mao Xuefeng | 2008 | Fine Chemicals2008，25，9： | 1 |
| 11 | Time-resolved photoluminescence studies of indium-rich InGaN alloy 显示文摘 | Chen Guangde Zhu Youzhang Yan Guojun Yuan Jinshe Kim K H Lin J Y Jiang H X | 2005 | Chin Phys Lett2005，22，2： | 1 |
| 12 | The geology and hy- drocarbon potential of petroliferous basins in China 显示文摘 | Li Desheng Liu Guangding Fu Jiamo | 1995 | Epi- sodes1995，18，12： | 1 |
| 13 | Study on Wear-preventing of Needle Valve in Fuel Injec- tion System Mounted on DME Engine显示文摘 | WEN Xinrong ZHANG Guangde WANG Weihua | 2011 | Advanced Mate- rials Research2011，，： | 1 |
| 14 | Geophysical and geological exploration and hydrocarbon prospects of the East China Sea显示文摘 | Liu Guangding | 1989 | China Earth Sciences1989，1，1： | 1 |
| 15 | An Intelligent Instrument for Ultrasonic Nondestructive Examination Haw Inspection of Rails显示文摘 | Guangde Song Xianglin Zhan Hui Zhang | 2004 | Proceedings of the 5''th World Congress on Intelligent Control and Automation2004，，6： | 1 |
| 16 | 显示文摘 | Fan Jinshe Chen Guangde Zhang Xianbin | 2003 | J Semiconductor Optoelectronics2003，24，： | 1 |
| 17 | The geology and hydrocarbon potential of petroliferous basins in China显示文摘 | Li Desheng Liu Guangding Fu Jiamo | 1995 | Episodes1995，18，12： | 1 |
| 18 | Study of atmospheric pressure microwave plasma spectrometry显示文摘 | Jin Qinhan Yang Guangde Yu Aimin | 1985 | Acta Scientiarum Naturalium Universitatis Jilinensis1985，，1： | 1 |
| 19 | 显示文摘 | Huimin L Guangde C Honggang Y | 2007 | J Appl Phys2007，101，5： | 1 |
| 20 | Land-Use Classification Using Taxi GPS Traces显示文摘 | Pan Gang Qi Guangde | 2013 | IEEE Transactions on Intelligent Transportation System2013，14，1： | 1 |
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