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| 1 | The USDA cucumber (Cucumis sativus L.) collection: genetic diversity, population structure, genome-wide association studies, and core collection development显示文摘Germplasm collections are a crucial resource to conserve natural genetic diversity and provide a source of novel traits essential for sustained crop improvement.Optimal collection,preservation and utilization of these materials depends upon knowledge of the genetic variation present within the collection.Here we use the high-throughput genotyping-by-sequencing(GBS)technology to characterize the United States National Plant Germplasm System(NPGS)collection of cucumber(Cucumis sativus L.).The GBS data,derived from 1234 cucumber accessions,provided more than 23 K high-quality single-nucleotide polymorphisms(SNPs)that are well distributed at high density in the genome(~1 SNP/10.6 kb).The SNP markers were used to characterize genetic diversity,population structure,phylogenetic relationships,linkage disequilibrium,and population differentiation of the NPGS cucumber collection.These results,providing detailed genetic analysis of the U.S.cucumber collection,complement NPGS descriptive information regarding geographic origin and phenotypic characterization.We also identified genome regions significantly associated with 13 horticulturally important traits through genome-wide association studies(GWAS).Finally,we developed a molecularly informed,publicly accessible core collection of 395 accessions that represents at least 96%of the genetic variation present in the NPGS.Collectively,the information obtained from the GBS data enabled deep insight into the diversity present and genetic relationships among accessions within the collection,and will provide a valuable resource for genetic analyses,gene discovery,crop improvement,and germplasm preservation. | Xin Wang Kan Bao Umesh K.Reddy Yang Bai Sue A.Hammar Chen Jiao Todd C.Wehner Axel O.Ramírez-Madera Yiqun Weng Rebecca Grumet Zhangjun Fei | 2018 | Horticulture Research2018，5，1： | 7 |
| 2 | Transcriptomic and metabolomic analyses of cucumber fruit peels reveal a developmental increase in terpenoid glycosides associated with age-related resistance to Phytophthora capsici显示文摘The oomycete,Phytophthora capsici,infects cucumber(Cucumis sativus L.)fruit.An age-related resistance(ARR)to this pathogen was previously observed in fruit of cultivar‘Vlaspik’and shown to be associated with the peel.Young fruits are highly susceptible,but develop resistance at~10–12 days post pollination(dpp).Peels from resistant(16 dpp)versus susceptible(8 dpp)age fruit are enriched with genes associated with defense,and methanolic extracts from resistant age peels inhibit pathogen growth.Here we compared developing fruits from‘Vlaspik’with those of‘Gy14’,a line that does not exhibit ARR.Transcriptomic analysis of peels of the two lines at 8 and 16 dpp identified 80 genes that were developmentally upregulated in resistant‘Vlaspik’16 dpp versus 8 dpp,but not in susceptible‘Gy14’at 16 dpp.A large number of these genes are annotated to be associated with defense and/or specialized metabolism,including four putative resistance(R)genes,and numerous genes involved in flavonoid and terpenoid synthesis and decoration.Untargeted metabolomic analysis was performed on extracts from 8 and 16 dpp‘Vlaspik’and‘Gy14’fruit peels using Ultra-Performance Liquid Chromatography and Quadrupole Time-of-Flight Mass Spectrometry.Multivariate analysis of the metabolomes identified 113 ions uniquely abundant in resistant‘Vlaspik’16 dpp peel extracts.The most abundant compounds in this group had relative mass defects consistent with terpenoid glycosides.Two of the three most abundant ions were annotated as glycosylated nor-terpenoid esters.Together,these analyses reveal potential mechanisms by which ARR to P.capsici may be conferred. | Ben N Mansfeld Marivi Colle Yunyan Kang A Daniel Jones Rebecca Grumet | 2017 | Horticulture Research2017，4，1： | 3 |
| 3 | Molecularly tagged genes and quantitative trait loci in cucumber with recommendations for QTL nomenclature显示文摘Cucumber,Cucumis sativus L.(2n=2x=14),is an important vegetable crop worldwide.It was the first specialty crop with a publicly available draft genome.Its relatively small,diploid genome,short life cycle,and selfcompatible mating system offers advantages for genetic studies.In recent years,significant progress has been made in molecular mapping,and identification of genes and QTL responsible for key phenotypic traits,but a systematic review of the work is lacking.Here,we conducted an extensive literature review on mutants,genes and QTL that have been molecularly mapped or characterized in cucumber.We documented 81 simply inherited trait genes or major-effect QTL that have been cloned or fine mapped.For each gene,detailed information was compiled including chromosome locations,allelic variants and associated polymorphisms,predicted functions,and diagnostic markers that could be used for marker-assisted selection in cucumber breeding.We also documented 322 QTL for 42 quantitative traits,including 109 for disease resistances against seven pathogens.By alignment of these QTL on the latest version of cucumber draft genomes,consensus QTL across multiple studies were inferred,which provided insights into heritable correlations among different traits.Through collaborative efforts among public and private cucumber researchers,we identified 130 quantitative traits and developed a set of recommendations for QTL nomenclature in cucumber.This is the first attempt to systematically summarize,analyze and inventory cucumber mutants,cloned or mapped genes and QTL,which should be a useful resource for the cucurbit research community. | Yuhui Wang Kailiang Bo Xingfang Gu Junsong Pan Yuhong Li Jinfeng Chen Changlong Wen Zhonghai Ren Huazhong Ren Xuehao Chen Rebecca Grumet Yiqun Weng | 2020 | Horticulture Research2020，7，1： | 2 |
| 4 | A quick and inexpensive method for removing polysaccharides from plant genomic DNA显示文摘 | Fang G Hammar S Grumet R | 1992 | BioTechniques1992，13，： | 1 |
| 5 | A quick and inexpensive method for removing polysaccharides from plant genemic DNA显示文摘 | Fang G Hammar S Grumet R | 1992 | Biotechniques1992，13，： | 1 |
| 6 | System for eleavable Fc fusion proteins using tobacco etch virus (TEV) protease 显示文摘 | Haspel J Blanco C Jacob J Grumet M | 2001 | Biotechniques2001，30，1： | 1 |
| 7 | Prevention of transfusion-acquired cytomegalovirus infections in newborn infants显示文摘 | Yeager A Grumet FC Hafleigh EB | 1981 | J Pediatr1981，98，2： | 1 |
| 8 | A quick and inexpensive method for removing polysaccharides from plant genomic DNA显示文摘 | FANG G HAMMAR S GRUMET R | 1992 | Biofeedback1992，13，1： | 1 |
| 9 | A quick and inexpensive method for removing polysaccharides from plant genomic DNA显示文摘 | Fang G Hammar S Grumet R | 1992 | Bio Techniques1992，13，： | 1 |
| 10 | Retrospective: Autobiography and the Analysis of Educational Experience 显示文摘 | Grumet M | 1990 | Cambridge Journal of Education1990，，3： | 1 |
| 11 | A quick and inexpensive method of removing polysaccharides from plant genomic DNA显示文摘 | Fang G Hammar S Grumet R | 1992 | Bioeedback1992，13，1： | 1 |
| 12 | A quick and inexpen- sive method for removing polysaccharides from plant genomic DNA显示文摘 | Fang G Hammar S Grumet R | 1992 | Bioteehniques1992，13，： | 1 |
| 13 | A quick and inexpensive method for removing polysaccharides from plant genomic DNA显示文摘 | Fang G Hammar S Grumet R | 1992 | BioTechniques1992，13，1： | 1 |
| 14 | Application of biosafety principles in blood establishments显示文摘 | Grumet F C Mac Pherson J L Hoppe P A | 1988 | Transfusion1988，28，5： | 1 |
| 15 | A Quick and Inexpensive Method for Removing Polysaccharides trom Plant (Se nomic DNA 显示文摘 | FANG G HAMMAR S GRUMET R | 1992 | Biotechniques1992，，13： | 1 |
| 16 | A quick and inexpensive method for removing polysaccharides from plant genomic DNA显示文摘 | FANG G HAMMAR S GRUMET R | 1992 | Bio Techniques1992，13，： | 1 |
| 17 | A quick and inexpensive method for removing polysaccharides from plant genomic DNA 显示文摘 | Fang G Hammar S Grumet R | 1992 | Biofeed- back1992，13，1： | 1 |
| 18 | A quick and inexpensive method for removing polysaccharides from plant genomic DNA显示文摘 | FANG G HAMMAR S GRUMET R | 1992 | BioTechniques1992，13，： | 1 |
| 19 | Arthoroscopic stabilization for first-time versus recurrent shoulder instability显示文摘 | Grumet RC Bach BR Jr Provencher MT | 2010 | Arthroscopy2010，26，2： | 1 |
| 20 | A quick and inexpensive method for removing polysaccharides from plant genomic DNA显示文摘 | Fang G Hammar S Grumet R | 1992 | Biofeedback1992，13，1： | 1 |
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